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Citations for Takara Bio :
151 - 200 of 1098 citations for Anti Flag Affinity Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Clarified supernatants were purified using a 5 mL Cobalt or Nickel affinity column (Takara). Purified protein was concentrated ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was applied to 4-mL Talon Metal Affinity Resin (Clontech, cat# 635503). After washing with 30 mL wash buffer containing 20 mM HEPES at pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... TsFpn was purified from the supernatant using a cobalt-based affinity resin (Talon, Clontech) and the His6-tag was cleaved by TEV protease at room temperature for 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: ... the mixture was incubated with pre-equilibrated of TALON Metal Affinity Resin (Takara, 635502) at 4°C for 1 h ...
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bioRxiv - Biochemistry 2022Quote: ... The dialyzed fraction was mixed for 3 h with Talon Metal Affinity Resin (Clontech) that had been equilibrated with Buffer T ...
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bioRxiv - Microbiology 2021Quote: ... The protein was purified from the supernatant by TALON metal affinity resin (Clontech, #635606) and eluted with 150 mM imidazole ...
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bioRxiv - Cell Biology 2020Quote: ... target proteins were purified from cell lysate by Ni-iminodiacetic acid affinity chromatography (Clontech). However ...
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bioRxiv - Biophysics 2021Quote: ... The resultant supernatant was mixed with 4 mg of Talon Metal Affinity Resin (Takara) equilibrated with wash buffer [50 mM Na-Pi pH 8.0 ...
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bioRxiv - Biophysics 2022Quote: ... and ACE2 was purified by nickel affinity chromatography using His60 Ni Superflow Resin (Takara) followed by size exclusion chromatography on a Superdex 200 column (Cytiva ...
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bioRxiv - Cell Biology 2022Quote: ... and the supernatant was incubated with 2 mL TALON Metal Affinity Resin (Takara Bio) for 1 h at 4 °C with rotation ...
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bioRxiv - Molecular Biology 2022Quote: ... Supernatant was incubated with equilibrated TALON Metal Affinity Resin (Takara Bio USA, Inc., CA) for 20 min on ice ...
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bioRxiv - Immunology 2023Quote: ... and supernatants were purified via immobilized metal affinity chromatography using Cobalt-TALON resin (Takara). Eluted proteins were further purified by size-exclusion chromatography using a Superdex 200 Increase 10/300 GL column (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was applied to 250 µL Talon metal affinity resin (Cat# 635652, TAKARA) previously washed twice with Equilibration buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... Centrifuged lysate was purified by gravity-flow chromatography on TALON Metal Affinity Resin (Clontech) following the manufacturer’s protocol with 0.05% Triton X-100 added to all buffers ...
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bioRxiv - Biochemistry 2023Quote: ... The cleared lysate was then incubated with 1ml of TALON metal affinity (Takara #Z5504N) resin according to manufacturer instruction ...
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bioRxiv - Molecular Biology 2024Quote: ... The expressed protein was then purified using TALON Metal Affinity Resin (Takara Cat. # 635502) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product was run on a 1% agarose gel at 85 V for 35 min and gel extracted using the Nucleospin Gel Extraction Kit (Takara). We then used PCR with KOD Polymerase 2x Mastermix (Millipore Sigma ...
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bioRxiv - Microbiology 2022Quote: ... products were gel-purified using Nucleospin gel and PCR Clean-up (Takara), and the sequences were determined by EuroFins Scientific.
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bioRxiv - Cancer Biology 2021Quote: ... After purification of the His6-SUMO-binder fusion proteins using TALON metal affinity resin (Clontech), they were digested with SUMO protease 1 ...
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bioRxiv - Microbiology 2020Quote: ... The Spike ectodomain was purified by immobilised metal affinity chromatography using Talon resin (Takara Bio) charged with cobalt followed by size exclusion chromatography using HiLoad 16/60 Superdex 200 column in 150 mM NaCl ...
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bioRxiv - Biophysics 2020Quote: ... and the supernatant was loaded onto a cobalt metal affinity column (Takara Bio, Kusatsu, Japan) equilibrated in CoWB ...
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bioRxiv - Microbiology 2022Quote: ... The purification was performed using TALON® Metal Affinity Resin from (Clontech Laboratories, Inc, 635501) with HisTALON™ Buffer Set (Clontech Laboratories ...
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bioRxiv - Biochemistry 2020Quote: ... and the supernatant was mixed with 1 mL of TALON® metal affinity beads (Clontech) previously equilibrated with equilibration buffer (PBS pH 8 ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant proteins of interest were bound to TALON metal affinity beads (Clontech, Mountain View, CA) and eluted with imidazole (gradient 10–200 mM ...
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bioRxiv - Biophysics 2021Quote: ... The solubilized protein was incubated with 2 mL TALON cobalt metal-affinity resin (Takara Bio) for 1 h ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant (soluble fraction) was applied to cobalt-loaded TALON Superflow metal affinity resin (Clontech) preconditioned in Sol A (20 mM NaPi ...
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bioRxiv - Microbiology 2020Quote: ... The Spike ectodomain was purified by immobilized metal affinity chromatography using Talon resin (Takara Bio) charged with cobalt followed by size exclusion chromatography using HiLoad 16/60 Superdex 200 column in 150 mM NaCl ...
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bioRxiv - Microbiology 2022Quote: ... and the supernatant was loaded into 0.5 ml of a Talon metal affinity resin (Clontech) equilibrated with binding buffer ...
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bioRxiv - Immunology 2022Quote: ... Cells were lysed by sonication and then purified via a TALON cobalt affinity resin (Clontech). The resin was washed with 40 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... the cleared lysates were incubated with 250 μL of TALON metal affinity resin (Clontech, 635502) for 1hr at 4°C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The trimeric HAs were purified from the supernatant using TALON metal affinity resin (Takara, #635653), washing with PBS ...
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bioRxiv - Microbiology 2022Quote: ... the supernatants were mixed with 100 μl of the Talon metal affinity resin (Clontech, USA) in a 1.5-ml tube ...
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bioRxiv - Neuroscience 2023Quote: ... Tau was then purified batchwise from the supernatant using TALON Metal Affinity Resin (TaKaRa, 635502) according to the vendor’s instructions ...
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bioRxiv - Physiology 2023Quote: ... Harvested conditioned media were incubated with Talon Metal Affinity resin (Clontech, Silicon Valley, CA, USA) at 4 °C overnight ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins were purified from Escherichia coli BL21 (DE3) with TALON Metal Affinity Resin (Takara), Pierce Glutathione Agarose (Thermo scientific) ...
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bioRxiv - Biophysics 2024Quote: ... The protein was purified to homogeneity using a combination of TALON Metal Affinity Resin (Takara) and gel filtration chromatography ...
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bioRxiv - Molecular Biology 2023Quote: ... The proteins were then purified using TALON metal-affinity resin (Clontech, Mountain View, California, USA) previously added and washed in PBS according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... and gel extracted using the NucleoSpin Gel and PCR Clean-up kit (Takara Bio USA Inc ...
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bioRxiv - Immunology 2021Quote: Flag-RNH1 was further sub-cloned into retroviral vector pMSCVpuro (Clontech). Retroviral vector pMSCVpuro-Flag–RNH1 was co-transfected with the helper plasmids VSV-G and Hit60 into HEK293T cells using PEI transfection reagent ...
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bioRxiv - Molecular Biology 2023Quote: ... C-FLAG-D20 was subcloned into the pLVX-TetOne-Puro (Clontech) and in-house attb vectors ...
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Ribonuclease Inhibitor and Angiogenin collaboratively regulate cell-type-specific global translationbioRxiv - Biochemistry 2024Quote: Flag-RNH1 was sub-cloned into the retroviral vector pMSCVpuro (Clontech) as described previously 21 ...
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bioRxiv - Cancer Biology 2021Quote: ... and then gel purified using NucleoSpin® Gel & PCR Clean-up Midi (Takara # 740986.20) for deep sequencing using the CRISPR-seq platform117 ...
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bioRxiv - Biochemistry 2019Quote: ... Clear supernatant was loaded onto a Ni-NTA affinity column containing 4ml Ni-NTA resin (Clontech) pre-equilibrated with lysis buffer at an approximate flow rate of 1ml/min ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was incubated with pre-equilibrated specific TALON® metal affinity resin (ClonTech, Scientifix, 635503) for 1 h with continuous stirring at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... and purified under denaturing condition (8 M urea) using the TALON metal affinity resin from Clontech. Purified fusion proteins were used to produce antibodies in guinea pigs from Covance Inc ...
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bioRxiv - Plant Biology 2020Quote: ... The E.coli-expressed tNPR1-His protein was extracted and purified using TALON Metal Affinity Resin (Clontech). For rabbit immunization ...
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bioRxiv - Bioengineering 2022Quote: ... The supernatant was mixed with equilibrated TALON Metal Affinity Resin (200 μL of slurry, Takara Bio) for 1 h at 4 °C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The recombinant Irg1-like protein was enriched using cobalt TALON Metal Affinity Resin (Takara Bio #635501) with gentle shaking for 1 hour at 4 ℃ and washed twice by centrifugation for 3 minutes at 3500 rpm at 4 ℃ and resuspension in the washing buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2019Quote: ... then 50,000g for 30 minutes and incubated with Talon Metal Affinity Resin (Clontech, Mountain View, CA) for 1 hour at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... in Terrific Broth and purified by metal ion affinity chromatography (IMAC) on TALON/Cobalt beads (Clontech) using 1M NaCl containing buffers to prevent the potential co-purification of E ...