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Citations for Takara Bio :
401 - 450 of 606 citations for Aminomethylphosphonic Acid 13C 99%;15N 98%;Methylene D2 98% 100 Ug Ml H2O since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... PCR was performed in 96-well plates and each well containing up to 10 µg of gDNA in a total of 100 µL reaction mixture consisting of Titanium Taq DNA polymerase buffer and enzyme (Takara, #639209), deoxynucleoside triphosphate ...
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bioRxiv - Neuroscience 2022Quote: ... EGFP-positive cells from control and Yap1-5SA groups were directly collected into ice-cold lysis buffer (0.2 %Triton X-100, containing RNAse inhibitors, Clontech, cat# 635013) in 96-well plates (Eppendorf ...
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Targeted attenuation of elevated histone marks at SNCA alleviates α-synuclein in Parkinson’s diseasebioRxiv - Neuroscience 2020Quote: ... the collected nuclei were pelleted down immediately and lysed in 100 μL RA1 buffer from the Nucleo Spin RNA XS Kit (Takara, 740902.50). This kit is optimized for isolating RNA from as little as one cell ...
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bioRxiv - Microbiology 2020Quote: ... 1 μl sense and 1 μl anti-sense primers of 100 μM each were mixed with 2 μl 10X Taq polymerase PCR buffer (Takara, Japan) and 16 μl ultra-pure water to a final volume of 20 μl ...
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bioRxiv - Genomics 2021Quote: ... The four PCR assays were run on a thermal cycler using 100 ng DNA with PrimeStar GXL PCR mastermix (Takara Bio) according to the manufacturer’s instruction using 58 °C annealing temperature ...
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bioRxiv - Cell Biology 2022Quote: hCEC were transduced with pHAGE-DD-KNL1Mut-dCas9 and a sgRNA vectors and DD-KNL1Mut-dCas9 was stabilized with 100 nM Shield-1 (Takara, #632189) for 9 days ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli were grown in 24 well plates with a 5 mm glass bead for aeration and BG11co was added to a total volume of 1500 µL at 500 µM IPTG and 100 nM of anhydrotetracycline final concentrations (aTc, Takara Bio). After 24 hours ...
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bioRxiv - Evolutionary Biology 2022Quote: ... anywhere between 20 to 100 ng of template per sample was used with PrimeSTAR GXL DNA Polymerase from TAKARA (Cat: R050B). The parameters for this 1st reaction were as follows ...
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bioRxiv - Immunology 2023Quote: ... A total of 100 ng isolated RNA was used for cDNA synthesis using PrimeScript High Fidelity RT-PCR Kit (Takara, R022B). RT-qPCR was performed using TB Green® Premix Ex Taq™ (Tli RNase H Plus ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cDNA was synthesized from 100 µg of total RNA by Prime ScriptTM RT reagent kit with gDNA Eraser (Takara, Tokyo, Japan). Each cDNA sample was amplified for the gene of interest and β-actin in a 15 µL reaction volume TB GreenTM Premix Ex Taq™ II (Takara ...
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bioRxiv - Developmental Biology 2023Quote: ... The following antibodies were used: rabbit or rat anti-dsRed (1:200, Clontech 632496 or 5F8 1:400, Chromotek 5f8-100); chicken anti-GFP (1:800 ...
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bioRxiv - Neuroscience 2020Quote: ... TetO/NGN2 gene expression was induced by Doxycycline (2 μg/mL; Clontech, Madison, WI) on day 0 ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant stirred with 10 mL of TALON metal affinity resin (Takara Bio US) equilibrated in Buffer A (480 mM NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 μL of Yeastmaker Carrier DNA (10 mg/mL, #630440, Takara Bio, Kusatsu, Japan), 1 μL of genome-editing plasmid (200–600 ng) ...
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bioRxiv - Biochemistry 2020Quote: ... Clarified supernatants were purified using a 5 mL Cobalt or Nickel affinity column (Takara). Purified protein was concentrated ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was applied to 4-mL Talon Metal Affinity Resin (Clontech, cat# 635503). After washing with 30 mL wash buffer containing 20 mM HEPES at pH 7.5 ...
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bioRxiv - Neuroscience 2021Quote: ... TDP-43-HA expression was induced by 1μg/ml of Doxycycline (DOX; Clontech #631311) when needed.
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bioRxiv - Microbiology 2022Quote: ... The resulting supernatant was supplemented with 0.75 mL pre-equilibrated TALON resin (Takara Clontech) and 1 mM imidazole and stirred for 30 min at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... The resulting supernatant was supplemented with 0.75 mL pre-equilibrated TALON resin (Takara Clontech) and 1 mM imidazole and stirred for 30 min at 4 °C ...
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... The p24 equivalent (ng/ml) was measured using Lenti-X GoStix Plus (Takara Bio) as per manufacturer instructions.
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bioRxiv - Microbiology 2021Quote: ... Dried membranes were pre-hybridized in 8 ml of pre-warmed ExpressHyb buffer (Clontech) for 1 h at 55°C before adding filtered 32P-labeled probes and incubating at 38.5°C overnight (Biometra OV5 ...
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bioRxiv - Immunology 2019Quote: ... non-tissue cultured treated 24 well plates were coated with 50μg/mL Retronectin (Takara) overnight and washed once with 1mL/well PBS ...
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bioRxiv - Biochemistry 2021Quote: ... wt and mutants was induced by treatment with 2 μg/ml Doxycycline (631311, Clontech) for 24-48 hours ...
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bioRxiv - Bioengineering 2022Quote: ... PDGF-aa (20 ng/ml; R&D) is also supplemented to RHB-A (Takara). This medium composition will be referred to as RHB-A complete ...
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bioRxiv - Cell Biology 2022Quote: ... and the supernatant was incubated with 2 mL TALON Metal Affinity Resin (Takara Bio) for 1 h at 4 °C with rotation ...
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bioRxiv - Developmental Biology 2024Quote: ... For beta-catenin overexpression the CHIR99021 was substituted with 1 µg/ml doxycycline (Clontech).
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bioRxiv - Developmental Biology 2019Quote: ... we washed the slides with PBT (this buffer was also used in subsequent washing steps) and incubated them in primary antibody (rabbit anti-DsRed, 1:100, Clontech, no. 632496) at 4 °C overnight ...
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bioRxiv - Microbiology 2022Quote: ... The resulting probe reaction mixtures were electrophoresed on a 0.8% agarose gel for 30 min at 100 V and then gel purified with a NucleoSpin Gel and PCR Clean-up kit (Takara Bio USA). The EMSA binding reaction ...
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bioRxiv - Physiology 2023Quote: ... cDNA was made from 100-400 ng of DNase-treated total RNA using a Takara PrimeScript RT Reagent Kit with gDNA Eraser (Takara bio RR047B). Quantitative PCR was performed using TB Green™ Premix Ex Taq™ (Tli RNaseH Plus ...
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bioRxiv - Plant Biology 2023Quote: ... an aliquot (100 µL) of transformed cells was then plated onto SD media containing the appropriate dropout supplement (Takara Bio USA, Inc.), and grown at 30°C for up to 7 days.
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bioRxiv - Microbiology 2023Quote: ... The protocol was as follows: PCR reaction mixture (100 nL) was prepared using SmartChip TB Green Gene Expression Master Mix (TaKara Bio, Japan), nuclease-free PCR-grade water ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Spec.100) and TB Green TM Premix Ex TaqTM II (Tli RNaseH Plus) (RR820A, Spec. 200) were supplied by Takara (Beijing, China). IP cell lysate (P0013 ...
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bioRxiv - Systems Biology 2023Quote: ... cDNA libraries for each sample were constructed from 100 ng of RNA using the PrimeScript RT master mix (Takara Bio, Kutatsu, Japan) under manufacturer specifications ...
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bioRxiv - Immunology 2019Quote: ... non-tissue culture treated 24-well plates were coated with 50 μg/mL Retronectin (Takara) overnight at 4 °C and washed once with PBS ...
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bioRxiv - Biophysics 2019Quote: ... The supernatant was incubated with 2 mL TALON resin (Takara, Saint-Germain-en-Laye, France) in a tube using a rotation shaker for 30-60 minutes ...
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bioRxiv - Genomics 2021Quote: ... MYC expression was induced in U2OS cells by the addition of 1µg/ml doxycycline (Clontech) for 30h.
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bioRxiv - Biophysics 2020Quote: ... and allowed to bind to cobalt resin (Talon, Clontech; 1 mL bed volume/L culture). The column was washed sequentially with cobalt wash buffer (CoWB ...
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bioRxiv - Biophysics 2020Quote: ... the lysate was pumped through a column containing 15 mL of Talon Cobalt resin (TAKARA). The resin was washed without using imidazole using a wash cycle consisting of low salt (20 mmol/L Tris Base ...
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bioRxiv - Biophysics 2022Quote: ... This was then loaded onto a column with 5 ml His60 Ni-Superflow Resin (Clontech) previously equilibrated in the lysis buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 ml of virus containing media were concentrated with Lenti-X concentrator (Takara Bio, 631231) to a titer sufficient to decrease pStat1 levels by 25% (Mean pStat1+ CD11b+ cells ...
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bioRxiv - Bioengineering 2022Quote: ... The resin was then packed in a TALON 2 mL Disposable Gravity Column (Takara Bio), and the column was washed three times with 3 mL of washing buffer (50 mM sodium phosphate ...
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bioRxiv - Cell Biology 2021Quote: ... CHO-K1 cells were co-treated using 1μg/mL doxycycline (Takara Bio Europe, Clontech Inc.) along with the small molecules for experiments as described in main text.
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bioRxiv - Biochemistry 2020Quote: ... and the supernatant was mixed with 1 mL of TALON® metal affinity beads (Clontech) previously equilibrated with equilibration buffer (PBS pH 8 ...
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bioRxiv - Biochemistry 2019Quote: ... Clear lysate was immediately passed through a 5 mL TALON™ Superflow cartridge (Takara Bio). After extensive washing with buffer A (50 mM HEPES pH 8.0 ...
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bioRxiv - Genomics 2020Quote: ... half of the cells were treated with 100ng/ml Doxycycline and 1μM Shield1 (Clontech Takara) for 15h ...
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bioRxiv - Genomics 2020Quote: ... half of the cells were treated with 100ng/ml Doxycycline and 1μM Shield1 (Clontech Takara) for 15h ...
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bioRxiv - Cancer Biology 2020Quote: ... wells were first coated with 100μl of RetroNectin solution (32μg/ml in PBS, Takara Bio), incubated for 2 hours at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... The solubilized protein was incubated with 2 mL TALON cobalt metal-affinity resin (Takara Bio) for 1 h ...
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bioRxiv - Biochemistry 2021Quote: ... Permeabilized cells were then mock treated or treated with 1 mg/ml RNase A (Takara) diluted in PBS for 20 minutes at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... CHO-K1 cells were co-treated using 1μg/mL doxycycline (Takara Bio Europe, Clontech Inc.) along with the small molecules for experiments as described in main text.