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Citations for Takara Bio :
501 - 550 of 606 citations for Aminomethylphosphonic Acid 13C 99%;15N 98%;Methylene D2 98% 100 Ug Ml H2O since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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X-ray mediated scintillation increases synaptic activity via Cerium-doped LSO and Channelrhodopsin-2bioRxiv - Neuroscience 2020Quote: ... showing a viral titer of >106 IFU/ml as measured by Lenti-X GoStix (Takara, Mountain View, CA). To develop stably expressing OptoXR-EYFP cells ...
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bioRxiv - Biochemistry 2022Quote: ... the cleavage mixture was applied to a gravity flow column packed with 8 mL TALON cobalt resin (Takara) equilibrated in AEX buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Matings between the Y2HGold and Y187 strains carrying the appropriate constructs were performed in 0.5 mL of 2X YPDA in the presence of the corresponding antibiotics following manufacturer’s instructions (Clontech’s Matchmaker Gold Yeast Two-Hybrid System ...
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bioRxiv - Genetics 2022Quote: ... 0.1-0.2*106 mESCs were seeded per well in a 24-12-well plate in conventional ESC medium and transduced the next day with 0.25-0.5 ml of 10:1 concentrated (lenti-X, Clontech) supernatant with 8 ng/μl polybrene (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: Nuclei were isolated from 1.5 × 109 DT40 cells and digested with 60 units/ml MNase (Takara Bio Inc.) in buffer A (15 mM Hepes-KOH ...
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bioRxiv - Cell Biology 2023Quote: ... cultures were split into two 2-ml cultures of which one was supplemented with 250 nM rapalog (Clontech). Mislocalisation of the target protein was verified by live-cell microscopy.
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bioRxiv - Immunology 2023Quote: Non-tissue culture treated 12-well plates were coated overnight at 4°C with 1 ml Retronectin (Takara) at 25 μg/ml in PBS ...
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bioRxiv - Genetics 2023Quote: ... and selection was performed on SD-Trp-Leu plates containing 200 ng/ml of Aureobasidin A (Clontech, 630466) according to the manufacturer’s instructions (Clontech) ...
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bioRxiv - Cancer Biology 2024Quote: ... 500 cells were collected in the center of a 1.5 ml centrifuge tube containing 4.75 μl SMART-seq reaction buffer (Takara) using a BD FACSymphony S6 (BD Bioscience) ...
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bioRxiv - Microbiology 2024Quote: ... The supernatants from the samples were added to 0.5 ml of a Talon metal affinity resin (Clontech, USA) equilibrated with binding buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were infected with concentrated lentivirus for 24 h in wells pre-coated with Retronectin (0.2 mg/ml; Takara) in expansion medium supplemented with protamine sulfate (10 μg/ml) ...
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bioRxiv - Developmental Biology 2019Quote: ... 200 ng of expression-ready Donor clone and acceptor Vector were recombined in a final volume of 10 µl for 15 minutes at 25 °C in a thermal cycler in the presence of Cre recombinase (0.2 units) and recombinase buffer supplemented with BSA (0.1 mg/ml) (Clontech #631614) according to Clontech manual PT3460-1 (now part of Takara Bio) ...
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bioRxiv - Genomics 2019Quote: ... We spun down the aliquots and re-suspended the pellets in 1 ml DroNc-seq resuspension buffer (1X PBS, 0.01% BSA, 0.16 U/μl RNase Inhibitor (Clontech/TaKaRa, #2313A)) for Tube A and 1 ml 10X Nuclei resuspension buffer (1X PBS ...
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bioRxiv - Immunology 2021Quote: ... Concentrated vectors were added at the indicated MOI (multiplicity of infection) in presence of retronectin at 10ug/ml (Takara). The plates were then centrifuged at 1,000 g for 1 h and incubated at 37°C during 3hrs ...
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bioRxiv - Biochemistry 2020Quote: ... The final supernatant was supplemented with NaCl to 150 mM and incubated with 3 mL His60 resin (Takara Biosciences) for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... cells were cultivated for three days in growth medium supplemented with 20 μg/ml of puromycin (Takara Bio Inc.) for PVR1 KO or 40 μg/ml for PVR2 KO ...
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bioRxiv - Neuroscience 2021Quote: ... 10 μM ROCK inhibitor (Y-27632; Selleckchem, Cat. No. S1049) and 2 μg/mL doxycycline (Clontech, Cat. No. 631311). Media was changed daily during this stage.
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Panacea: a hyperpromiscuous antitoxin protein domain for the neutralisation of diverse toxin domainsbioRxiv - Microbiology 2021Quote: ... Filtered lysate was incubated with 1 mL of previously buffer equilibrated Ni-beads (His60 Ni Superflow Resin, TaKaRa, Japan) for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... The culture was split into two 2-mL cultures of which one was supplemented with 250 nM rapalog (Clontech). Parasite growth was determined via flow cytometry over five days as described above ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was clarified by centrifugation (292,055 g, 60 min, 4°C) and bound to 2 ml of TALON IMAC resin (Clontech) overnight with 10 rpm rotation in the presence of 20 mM imidazole and NaCl added up to 800 mM ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were washed twice with 20 mL of D-PBS (Nacalai Tesque) and resuspended in CELLBANKER 1 (Takara Bio) at 1 × 107 cells/mL ...
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bioRxiv - Molecular Biology 2022Quote: ... The BAC containing Idh was extracted from 200 ml bacterial cultures using the NucleoBond Xtra Midi kit (Takara Bio). The fluorescence in situ hybridization (FISH ...
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bioRxiv - Biochemistry 2021Quote: ... prepared as described above was mixed with imidazole (5 mM) and 0.5 mL of Talon superflow metal affinity resin (Clontech) that had been equilibrated with buffer (as above for magnetic agarose) ...
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bioRxiv - Molecular Biology 2021Quote: ... The insoluble material was removed by centrifugation at 150,000 ×g and the supernatant was added to 1 ml pure TALON resin (Clontech) and 20 mM imidazole and rock slowly overnight at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... His-tagged NTD domain constructs were purified from clarified supernatants using 2 ml of cobalt resin (Takara Bio TALON), washing with 50 column volumes of 20 mM HEPES-HCl pH 8.0 and 150 mM NaCl and eluted with 600 mM imidazole ...
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bioRxiv - Immunology 2021Quote: ... The supernatants were collected and applied to a column filled with 5 ml of TALON Metal Affinity resin (Takara) previously equilibrated with ice-cold PBS ...
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bioRxiv - Immunology 2021Quote: ... and transduced to NK-92MI cells in a 24-well plate coated with 0.5 µg/ml of RetroNectin diluted in PBS (Clontech). Two days later ...
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bioRxiv - Immunology 2020Quote: ... non-tissue culture treated 96-well plates were coated overnight at 4 °C with 1 ml of retronectin (Takara) at 25μg/ml in PBS ...
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bioRxiv - Immunology 2020Quote: ... CR3022-CAR retrovirus was harvested after 48-72 hours and transduced to NK92MI cells in a 24-well plate coated with 0.5 μg/ml of RetroNectin diluted in PBS (Clontech). Two days later ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant with the soluble His6-UppH was loaded on to a 3 ml TALON Metal Affinity Resin (Clontech) equilibrated in binding buffer (50 mM Na2HPO4 ...
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bioRxiv - Immunology 2021Quote: ... Retroviral supernatants were loaded by centrifugation (2,000xg for 2 hours at 30°C) onto non-tissue culture treated 6-well plates pre-coated with RetroNectin (20μg/mL; Takara). Activated T cells were added and spin-transduced for 30 minutes at 1,000xg ...
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bioRxiv - Pathology 2023Quote: ... Cells were harvested by centrifugation at 4,000 rpm for 20 min at 4℃ and resuspended in 20 ml xTractor buffer (Clontech, TaKaRa Biomedical Technology [Beijing] Co. ...
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bioRxiv - Pathology 2023Quote: ... Cells were harvested by centrifugation at 4,000 rpm for 20 min at 4℃ and resuspended in 20 ml xTractor buffer (Clontech, TaKaRa Biomedical Technology [Beijing] Co. ...
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bioRxiv - Pathology 2023Quote: ... The bacteria were then collected by centrifugation at 4,000 rpm for 5 min and 2 ml xTractor Buffer (Clontech, TaKaRa Biomedical Technology [Beijing] Co. ...
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bioRxiv - Pathology 2023Quote: ... The bacteria were then collected by centrifugation at 4,000 rpm for 5 min and 2 ml xTractor Buffer (Clontech, TaKaRa Biomedical Technology [Beijing] Co. ...
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bioRxiv - Cell Biology 2022Quote: ... Mav203 was transformed with four plasmids and spread onto Sc-LeuTrpUra plates containing 0.5 μg/ml Aureobasidin A (TAKARA).
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bioRxiv - Cancer Biology 2023Quote: ... His-TEV tagged protein was captured with Co-TALON resin (Clonetech, Takara Bio USA, 2 mL slurry/liter culture) at 4 ºC for 1 h with constant end-to-end mixing ...
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bioRxiv - Microbiology 2023Quote: ... The protein-bead mixtures were then loaded onto a gravity column (TALON 2 mL Disposable Gravity Column, Takara #635606). Lysate was loaded into the column 5 times with gravity flow and then washed in a 1M NaCl buffer 3 times ...
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bioRxiv - Neuroscience 2024Quote: ... in BrainPhys neuronal media (composed according to Bardy et al. 2015)74 and 2 µg/ml doxycycline (Takara 631311). Three days after plating and ...
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bioRxiv - Microbiology 2024Quote: ... the dish was split into two 1-ml Petri dishes and one was treated with rapalog (250 nM, Clontech) for 4 hours to induce the knock-sideway while the other served as control.
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bioRxiv - Cell Biology 2020Quote: ... to a final concentration of 108-109 IFU/ml using the Lenti-x qRT-PCR Titration Kit (Takara, cat.631235). Lentivirus with MOI of 10 was used for transduction of MOVAS cells ...
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bioRxiv - Cell Biology 2020Quote: Flp-In™ T-REx™ 293 cell lines stably transfected with Flag-BirA*-TopBP1 WT or W1145R grown to 75% confluence were incubated with 1µg/ml of doxycycline (Clontech, 631311) for 16h and with 50µM biotin for 3 or 16 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... The cell lines of each genotype were tested for CARD14 expression in the presence of 500 ng/ml Dox (Clontech), and clones with the highest fold induction were selected for further analyses.
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bioRxiv - Genomics 2019Quote: ... We spun down the aliquots and re-suspended the pellets in 1 ml DroNc-seq resuspension buffer (1X PBS, 0.01% BSA, 0.16 U/μl RNase Inhibitor (Clontech/TaKaRa, #2313A)) for Tube A and 1 ml 10X Nuclei resuspension buffer (1X PBS ...
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bioRxiv - Neuroscience 2021Quote: ... 0.3 grams or more of brain tissue was thawed on ice for 5 minutes with 5 ml of nuclei buffer (NB): 1% BSA containing 0.2 U μl−1 RNase inhibitor (Takara, 2313A) and EDTA-free Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... Expression of shRNA targeting and knocking-down Eklf was induced by the addition of 2 µg/ml of doxycycline (Clontech) for 96 hr ...
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bioRxiv - Biochemistry 2022Quote: ... The precipitated material was removed by centrifugation and the soluble fraction was loaded onto a column (5 mL) containing Talon affinity resin (Clontech) equilibrated in buffer A ...
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bioRxiv - Neuroscience 2021Quote: ... Then the nuclei were centrifuged at 500 x g for 5 minutes at 4°C and washed in 4 ml Nuclei Suspension Buffer (NBS; consisting of 1× PBS, 0.04% BSA and 0.1% RNase inhibitor (Clontech, Cat #2313A)) ...
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bioRxiv - Plant Biology 2021Quote: ... 0.1g of leaves or fruits was mixed with 1 mL RNA iso plus according to manufacturer’s instruction (Takara, Kusatsu, Japan), then RNA was reverse-transcribed into cDNA using PrimeScript RT reagent with gDNA Eraser (Takara ...
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bioRxiv - Systems Biology 2020Quote: ... 24 hours before UV-crosslinking expression of the FLAG-RBM4 transgene was induced by addition of 200 ng/mL doxycycline (Takara) and 16 hours (or 1 hours ...