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Citations for Takara Bio :
1 - 50 of 1168 citations for Adenovirus Type 5 Particles CMV GFP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The CMV-GFP construct was pEGFPC1 (Clontech). HIV-1 bearing a Ba-L envelope gene has been described (Rasaiyaah et al. ...
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bioRxiv - Microbiology 2022Quote: ... The recombinant adenovirus was produced using an Adenovirus Dual Expression Kit (Takara Bio) in accordance with the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... The untagged version was made by cloning the RBP2 cDNA from this plasmid and replacing RIBEYE-GFP in the pAAV-CMV-HBA-RIBEYE-GFP-WPRE plasmid by in-fusion cloning (Takara Bio USA, Inc.).
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bioRxiv - Molecular Biology 2023Quote: ... in which the CMV promoter of pAAV-CMV vector (Takara Bio) was replaced by gfaABC1D ...
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bioRxiv - Microbiology 2020Quote: ... CSII-CMV-MCS-IRES2-Bsd (RIKEN BRC) and pLVSIN-CMV Pur (Takara Bio), respectively ...
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bioRxiv - Cell Biology 2020Quote: ... Adenovirus expressing human SPARC was constructed using Adeno-X expression system (Clontech) as described before 30,31 ...
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bioRxiv - Developmental Biology 2020Quote: ... It was subsequently cloned into BamHI site in the pLenti CMV-IRES-GFP by In-Fusion cloning kit (Clontech, 639649) to generate lentiviral packaging plasmids(pLentiCMV-Y437H-IRES-GFP) ...
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bioRxiv - Cancer Biology 2019Quote: ... The cDNA was then ligated into pCDH-CMV-MCS-EF1-GFP using Takara ligation Kit (Clontech, Mountain View, California, USA) and was confirmed in the expression vector by DNA sequencing at The Centre for Applied Genomics (SickKids ...
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bioRxiv - Cell Biology 2022Quote: ... All above sequences along with MBNL1 and MBNL2 isoforms imaged in primary neurons were cloned downstream of GFP in a pEGFP-C1 plasmid backbone under control of a CMV promoter (Takara). Full-length kinesin sequences were amplified from mouse cDNA and inserted upstream of an mScarlet-containing plasmid in a pcDNA3.1 backbone under the control of CMV promoter.
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bioRxiv - Developmental Biology 2020Quote: ... The CMV-MitoDsRed2 (pDsRed2-Mito) and CMV-MitoGFP (pAcGFP1-Mito) plasmids are from Clontech. Atoh7-MitoDsRed2 was designed using UGene software (Unipro) ...
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bioRxiv - Immunology 2020Quote: ... 1.3 μg CMV 8.91 (Clontech), and 1.5 μg of target vector were transfected using TransIT-293T transfection reagent (Mirus Bio) ...
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bioRxiv - Developmental Biology 2019Quote: ... The SOX2 PCR product was purified and inserted into CMV-t2a-GFP using the In-Fusion HD cloning kit (Takara Bio USA) and transformed into Stellar Competent Cells (Takara Bio USA) ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... and CCR6high and transduced with lentivirus particles at an MOI of 5 using retronectin (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: The lentivirus-based expression plasmids were generated with a pLOV-CMV-GFP vector (Neuron Biotech, China) using In-Fusion HD Cloning kits (Clontech Laboratories, Inc., USA), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: Adenovirus vectors were generated with the Adeno-X Adenoviral System 3 following manufacturer’s instructions (Takara, #632267). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: ... The titer of adenovirus was examined using an Adeno-XTM Rapid Titer Kit (Takara Bio USA) and determined to be 3.4 × 1011 ifu/ml ...
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bioRxiv - Molecular Biology 2022Quote: ... These coding gene sequences were all cloned into CMV-based FLAG-tagging and Clover-tagging vectors (CMV-FLAG-J1 and CMV-Clover-J1; derived from commercially available vectors pEGFP-C1 and pEGFP-N1; Clontech) for ectopic expression in human cells.
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bioRxiv - Neuroscience 2022Quote: ... Mover-IRES-palmGFP (promoter: CMV; backbone: Clontech N1); mGFP-Mover (promoter ...
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bioRxiv - Neuroscience 2020Quote: ... the CMV-EGFP plasmid (pEGFP; Clontech, CA, USA) was used as a positive control ...
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bioRxiv - Neuroscience 2023Quote: ... anti-GFP (Clontech, JL-8, 1:10,000 in 5% non-fat milk) and anti-GAPDH (Millipore ...
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bioRxiv - Synthetic Biology 2023Quote: ... VH and VL segments were ordered as gene blocks from Integrated DNA Technologies and were cloned into linearized CMV/R backbones with 5× In-Fusion HD Enzyme Premix (Takara Bio).
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bioRxiv - Physiology 2021Quote: ... Adenovirus was generated in HEK293A cells and titered using the Adeno-X Rapid Titer Kit (Takara Bio). For differentiation ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were transiently transfected either with GluK3EM or co-tansfected with Wild type/mutant receptors along with GFP expressing plasmid (2 µg/dish) using Xfect reagent (Clontech). Currents were recorded from medium sized cells expressing a moderate level of fluorescence from either the fused EGFP in case of GluK3EM or co-expressed EGFP and having a capacitance of ∼5-6 pF at 48-60 hours post transfection ...
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bioRxiv - Cell Biology 2022Quote: ... the CMV promoter from pEGFP-C1 (Takara Bio Inc) was removed and replaced with the SEPTIN2 promoter using AseI and AgeI ...
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bioRxiv - Cancer Biology 2024Quote: ... pHTN HaloTag® CMV-neo and pHTC HaloTag® CMV-neo were digested with restriction enzymes and subsequently ligated (Takara, cat n°6023). Primers and corresponding restriction digests can be found in Table S4 ...
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bioRxiv - Plant Biology 2022Quote: ... milk 5%) and incubated with a 2000-fold dilution of anti-GFP (JL8; Clontech), anti-RGA (Agrisera) ...
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bioRxiv - Immunology 2022Quote: ... Recombinant adenovirus vaccines were produced using the Adeno-X™ Adenoviral System 3 according to the manufacturer’s manual (Takara Korea Biomedical Inc. ...
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bioRxiv - Microbiology 2022Quote: ... The recombinant adenovirus was prepared in HEK293 cells and purified with an Adeno-X Virus Purification kit (Takara Bio). The purified virus titer was determined using an Adeno-X Rapid Titer kit (Takara Bio) ...
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bioRxiv - Cell Biology 2021Quote: ... pEGFP and mCherry vectors were from Clontech (with CMV promoter) with either C1 or N1 cloning sites to obtain the tag either at the N-terminus or C-terminus of Arc ...
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bioRxiv - Immunology 2021Quote: ... wild-type SARS-CoV-2 RBD was purified using a 5 mL HisTALON superflow cartridge (Takara Bio) followed by size exclusion chromatography using a Superdex 200 10/300 GL column pre-equilibrated in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Physiology 2023Quote: ... The adenovirus titer in plaque-forming units (PFU) was determined using a Adeno-X Rapid Titer kit (Clontech, Palo Alto, CA).
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bioRxiv - Genomics 2023Quote: ... 5 µm-thick sections were cut and immunostained with anti-GFP antibody (living colors, Clontech, 632592) and secondary anti-rabbit IgG with Alexa fluor 488 (Thermo Fisher) ...
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bioRxiv - Microbiology 2020Quote: ... GFP (Clontech), GAPDH (Cell Signaling Technology) ...
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bioRxiv - Microbiology 2022Quote: ... GFP (Clontech), PKR ...
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bioRxiv - Cell Biology 2022Quote: ... GFP (Clontech), and β actin (Sigma ...
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bioRxiv - Plant Biology 2020Quote: ... GFP (Takara), SUMO3 (Agrisera) ...
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bioRxiv - Microbiology 2022Quote: ... GFP (Clontech), PABPC1 and ICP4 (Santa Cruz) ...
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bioRxiv - Microbiology 2024Quote: ... GFP (Clontech), PABPC1 (Santa Cruz ...
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bioRxiv - Biophysics 2021Quote: ... viral particles produced by transfecting LentiX-293T (Takara Bio) cells with the sgRNA lentiviral vector were added to the dual stable cell lines ...
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bioRxiv - Cell Biology 2020Quote: ... Viral particles were precipitated using Retro-X Concentrator (Clontech) and resuspended in STEMdiff Neural Progenitor Medium containing 8 μg/ml Polybrene (EMD Millipore) ...
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bioRxiv - Cell Biology 2022Quote: ... AAV particles were isolated using AAVPro extraction solution (Clontech). After transduction with both CRISPR/Cas9 gesicles and AAV homology donor ...
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bioRxiv - Genomics 2021Quote: ... Lentiviral particles were produced in 293T-Lenti-X (Clontech) cells cultured in DMEM ...
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bioRxiv - Cell Biology 2023Quote: Lentiviral particles were generated using the pLVX system (Clontech) with packaging vectors psPAX2 and pMD2.G (Addgene plasmids #12260 and #12259) ...
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bioRxiv - Immunology 2022Quote: ... Lentiviral particles were produced in Lenti-X cells (Clontech) per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentiviral particles were concentrated using the LentiX Concentrator (Clontech). Organoids were trypsinized into small cell clusters and incubated with the concentrated virus particles supplemented with 4 μg/mL Polybrene (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... Cousin lab was dropped into an pEGFP-N1 CMV mammalian expression vector (Clontech) by In-fusion cloning ...
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bioRxiv - Neuroscience 2024Quote: The mammalian expression vector pBApo-CMV-Pur (cat. #3421) was purchased from Takara. We used Genescript to insert the 144 amino acid sequence for WT synuclein into the backbone and create 6 mutated strains ...
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bioRxiv - Genomics 2023Quote: ... Primary antibodies were diluted in 5% milk in PBS-T + 0.01% sodium azide (1:2,000 for mouse anti-GFP (Clontech) and mouse anti-3V5 (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... procollagen type I (Takara #MK101), fibronectin (Takara #MK115) ...
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bioRxiv - Cell Biology 2022Quote: ... Lentiviral particles were further concentrated using Lenti-X concentrator (Clontech) following the manufacturer’s instructions.