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Citations for Takara Bio :
351 - 400 of 1168 citations for Adenovirus Type 5 Particles CMV GFP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... Lentiviral particles were concentrated from supernatant by mixing 3 parts supernatant with 1 part Lenti-X concentrator solution (ClonTech 631231), incubating overnight at 4 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... Retrovirus supernatant or medium containing virus particles was harvested at day 2 post transfection and concentrated by Retro-Concentrator (Clontech) solution ...
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bioRxiv - Cell Biology 2022Quote: ... VSV-G pseudotyped retroviral particles encoding constructs of choice were prepared using standard protocols using GP2-293 packaging cells (Clontech). Viral supernatants were collected and used to infect target cells by centrifugal inoculation (spinoculation) ...
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bioRxiv - Cancer Biology 2020Quote: ... Human melanoma cell lines and Hema-LP were overlaid with viral particles diluted in DMEM/1x Glutamax with 10% TET System Approved FBS (Clontech) supplemented with 5 μg/ml polybrene (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2019Quote: ... and lentiviral Tre3G-Cas9 and Tet3G particles were produced in HEK293T cells using the Lenti-X HT packaging system (Clontech). Transduced cells were selected with 10 μg/mL puromycin and 400 μg/mL G418 ...
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bioRxiv - Cancer Biology 2022Quote: Viral particles were generated by transfecting the lentiviral construct into HEK-293T cells using Lenti-X Packaging Single Shots (Clontech). 48 hours after transfection ...
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bioRxiv - Immunology 2022Quote: ... Concentrated particles were resuspended in 100% WRN conditioned media and titers were checked using Lenti-XTM GoStixTM Plus (Takara 631280). Only high titer lenti-particles were used to transduce duodenoids ...
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bioRxiv - Molecular Biology 2023Quote: ... the supernatants of infected cells were collected and infectious virus particles were measured using the Adeno X Rapid Titration Kit (Takara Bio Europe ...
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bioRxiv - Molecular Biology 2023Quote: ... and HEK293-FT cells were used to prepare lentiviral particles according to the instructions in the Lenti-XTM Lentiviral Expression System Manual (Clontech). Doxycycline-inducible ZDHHC20 expressing HEK293T cells were prepared by transduction of 2.5 X 105 low-passage cells with lentivirus ...
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bioRxiv - Genomics 2024Quote: ... The cells were infected with prepackaged lentiviral particles (constitutive reporter vector expressing tdTomato fluorescent protein gene driven by EF1a promoter (Takara) at MOI of 20 (Stock ...
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bioRxiv - Cancer Biology 2024Quote: ... with psPAX2 and pMD2.G viral particles harvested during the 16-72 h window post-transfection were concentrated with Lenti-X (Clontech) and stored at -80°C ...
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bioRxiv - Neuroscience 2020Quote: ... 341F (5’-CCTACGGGNGGCWGCAG-3’) and 805R (5’-GACTACHVGGGTATCTAATCC-3’) or (2) 341F (5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG-3’) and 806R (5’-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGGACTACHVGGGTWTCTAAT-3’) (TaKaRa Bio, Shiga, Japan). The second PCR was performed to add the index sequences for Illumina sequencing with barcode sequences ...
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bioRxiv - Microbiology 2019Quote: ... purchased antisera used were: mouse monoclonal anti-GFP (clone JL8, Clontech), rat monoclonal anti-ß tubulin (clone YL1/2 ...
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bioRxiv - Neuroscience 2020Quote: ... The following primary antibodies were used: GFP (JL-8, Clontech, #632381), Tubulin (Covance ...
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bioRxiv - Cancer Biology 2021Quote: ... the GFP-expressing bicistronic reporter construct pIRES2-EGFP (BD Biosciences Clontech) was used ...
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bioRxiv - Cell Biology 2021Quote: ... JL-8 anti-GFP monoclonal antibody (TaKaRa, catalog # 632381, lot # A8034133) at 1:2,000 dilution ...
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bioRxiv - Plant Biology 2020Quote: ... anti-GFP (Living Colors® A.v. Monoclonal Antibody, JL-8; Clontech), anti-CHS (sc-12620 ...
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bioRxiv - Plant Biology 2021Quote: ... membranes were incubated in anti-GFP (Takara Bio, 1:5000 dilution) antibodies followed by a 2 h incubation in secondary goat anti-mouse-HRP (1:10,000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... Monoclonal mouse anti-GFP antibodies (JL-8) were from Clontech (632381). Monoclonal mouse anti-Flag (M2 ...
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bioRxiv - Cancer Biology 2022Quote: The following plasmids were used: GFP and mCherry (Clontech/Takara Bio); GFP-fascin WT/S39A/S39D (Zanet et al. ...
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bioRxiv - Cancer Biology 2022Quote: The following plasmids were used: GFP and mCherry (Clontech/Takara Bio); GFP-fascin WT/S39A/S39D (Zanet et al. ...
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bioRxiv - Immunology 2022Quote: ... mouse anti-GFP clone JL-8 (Takara Bio Cat# 632380, RRID:AB_10013427), rabbit anti-FLAG clone D6W5B (Cell Signaling Technology Cat# 14793 ...
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bioRxiv - Plant Biology 2021Quote: ... immunoblot with an anti-GFP antibody (1:5000 dilution, Takara Bio) followed by and detection were performed as described (Basu ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated with primary antibodies: mouse anti-GFP (1:2000; Clontech), mouse anti-FLAG (1:1000 ...
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bioRxiv - Synthetic Biology 2019Quote: ... and GFP-expressing vector (pEGFP-C1, Clontech, Mountain View, CA, USA) were from lab storage ...
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Receptor-like role for PQLC2 amino acid transporter in the lysosomal sensing of cationic amino acidsbioRxiv - Cell Biology 2020Quote: ... GFP-WDR41 fusions were constructed in the pEGFP-N1 plasmid (Clontech) and the GST-WDR41 fusion was generated in pGEX-6T-1 (GE Life Sciences) ...
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bioRxiv - Biochemistry 2019Quote: ... Anti-GFP (#632381) used for western blot was purchased from Clontech. Anti-GFP used for IP was purchased from Invitrogen (#G10362) ...
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bioRxiv - Cell Biology 2019Quote: ... followed by western blot using an anti-GFP antibody (632381, Clontech).
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bioRxiv - Cell Biology 2020Quote: All GFP plasmids used were cloned into pEGFP-N1 plasmid (Clontech): pEGFP-N1 ...
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bioRxiv - Genetics 2021Quote: ... probed with anti-GFP (Takara Bio Clontech, #632380, mouse, 1:5000) and anti-Tubulin (Sigma ...
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bioRxiv - Genetics 2021Quote: ... probed with anti-GFP (Takara Bio Clontech, #632380, mouse, 1:5000) and anti-Tubulin (Sigma ...
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bioRxiv - Developmental Biology 2020Quote: ... Westerns were probed with mouse monoclonal anti-GFP JL8 (#632381, Clontech) or mouse monoclonal anti-Myc 9E10 (DSHB).
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bioRxiv - Cell Biology 2020Quote: ... Living colors mouse anti-GFP monoclonal antibody was purchased from Clontech, and goat anti-mouse antibody HRP-conjugated antibody was purchased from Jackson ImmunoResearch.
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bioRxiv - Plant Biology 2021Quote: ... anti-GFP (Living Colors® A.v. Monoclonal Antibody, JL-8; Clontech), anti-HY5 (Oravecz et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were then incubated with anti-GFP antibody (JL-8; Clontech) at a dilution of 1:5,000 ...
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bioRxiv - Plant Biology 2023Quote: ... Anti-GFP (monoclonal antibody raised in mouse, Takara, USA, catalog # 632381) and anti-SEC12 (Bar-Peled and Raikhel ...
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bioRxiv - Cell Biology 2023Quote: ... GFP (Living Colors, Takara Biotech, San Jose CA, 632375; 1:2000), Pfn1 (Abcam ...
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bioRxiv - Plant Biology 2024Quote: ... We then ran a western blot using the GFP antibody (Takara living colors EGFP monoclonal antibody JL-8 ...
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bioRxiv - Plant Biology 2024Quote: ... equilibrated with 2 μg of anti-GFP antibody (Takara Bio, 632381). Beads were washed for 2 x 10 mins in low salt wash buffer ...
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bioRxiv - Immunology 2022Quote: Genetically modified NK cells were generated as follows: Supernatants containing retroviral particles encoding CAR constructs were spun at 2000g in retronectin (T100B, Takara Bio) coated non-tissue culture 24-well plates for 90 minutes ...
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bioRxiv - Immunology 2021Quote: ... activated CD8+ T cells were transduced with MSCV-ASNS-OE or MSCV-EV viral particles on RetroNectin-coated plates (Takara Bio). 24 hours later ...
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bioRxiv - Immunology 2022Quote: ... Retroviral vectors were packaged into VSV G–pseudotyped murine leukemia virus (MLV) particles by co-transfecting GP2-293 cells (Clontech Laboratories) with pQCXIP constructs and pVSV-G (Clontech Laboratories) ...
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bioRxiv - Molecular Biology 2023Quote: ... The viral particles were purified from transfected cells and titrated using the AAVpro Purification Kit Maxi and AAVpro Titration Kit (Takara Bio) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... The virus particles containing media were collected 24 h and 48 h after transfection and were concentrated using Lenti-X concentrator (Takara, 631232). The virus containing media was resuspended in Neurobasal media and tittered by puromycin selection using HEK 293T cell line ...
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bioRxiv - Neuroscience 2024Quote: ... was purchased from Clontech (Clontech; #P3070-5). Plasmids were prepared using the ZymoPURE Plasmid MaxiPrep Kit (Zymo Research ...
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bioRxiv - Neuroscience 2019Quote: ... replication-defective adenoviral vectors expressing dominant wild type FoxO3a were designed and purchased from TaKaRa Biotechnology (Dalian ...
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bioRxiv - Cancer Biology 2021Quote: ... The wilde-type (WT) hTERT-immortalized retinal pigment epithelium (hTERT-RPE1, Clontech, Palo Alto, CA) and RPE1–MYCN-ER were cultured in DMEM/F-12 HEPES ...
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bioRxiv - Cell Biology 2022Quote: Full length wild type human RORβ (NM_006914.3) was subcloned into the pLPCX retroviral vector (Clontech) using XhoI and NotI restriction enzymes (NEB ...
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bioRxiv - Biochemistry 2024Quote: The Upf1-HD-His6 wild-type protein was first purified on a TALONTM resin (Clontech) equilibrated with buffer A200 ...
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bioRxiv - Physiology 2022Quote: ... 5’-RACE was performed by SMARTer RACE 5’/3’ kit (Clontech) by manufacture protocol ...