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Citations for Takara Bio :
51 - 100 of 776 citations for Adenovirus Type 5 Particles CMV β galatosidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... The viral particles were concentrated using Lenti-X Concentrator (Takara Bio) and resuspended in TSM base and stored at −80°C for future use ...
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bioRxiv - Molecular Biology 2021Quote: ... Lentiviral particles were concentrated using Lenti-X concentrator (631232 Takara Bio) to a final volume 200 μl ...
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bioRxiv - Genetics 2022Quote: ... Lentiviral shRNA particles were prepared with Lenti-X 293T cells (Takara) and MISSION pLKO.1 plasmids (Sigma-Aldrich ...
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bioRxiv - Genomics 2019Quote: ... Lentiviral particles were concentrated ∼100 times using Lenti-X Concentrator (Takara). LUHMES cells were seeded in a 12-well-plate format and immediately transduced with 30 ul of virus concentrate ...
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bioRxiv - Neuroscience 2022Quote: ... Viral particles were concentrated using the Lenti-X Concentrator (Clontech, 631232) and the viral pellets were resuspended in PBS for astrocyte infection.
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bioRxiv - Cell Biology 2022Quote: ... Viral particles were generated using Lenti-X 293T cells (Takara; 632180) cultured in DMEM with 10% heat inactivated fetal bovine serum (MilliporeSigma ...
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bioRxiv - Cell Biology 2022Quote: ... and viral particles were precipitated using Lenti-X concentrator (Takara Bio) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Lentiviral particles were further concentrated using Lenti-X Concentrator (Takara, 631231). After that ...
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bioRxiv - Cancer Biology 2023Quote: ... Viral particles were concentrated using Lenti-X Concentrator (Takara Bio, #631232), and cells were transduced at a high multiplicity of infection with different polybrene concentrations (8ug/mL-30ug/mL).
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bioRxiv - Cell Biology 2024Quote: ... and lentiviral particles were concentrated using the Lenti-X Concentrator (Takara) as per the instructions ...
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bioRxiv - Biophysics 2021Quote: ... Protein constructs were expressed under control of a CMV promoter using pEGFP-C1/N1 (Clontech) (enhanced GFP ...
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bioRxiv - Developmental Biology 2019Quote: ... Supernatants with lentiviral particles were concentrated using lenti-X-concentrator (Takara, 631231) in 1:100 ratios ...
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bioRxiv - Biophysics 2020Quote: ... and the viral particles were precipitated using Lenti-X concentrator (Takara Bio) following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2020Quote: ... Lentiviral particles were produced in Gesicle Producer 293T cells (TAKARA ref 632617) transiently transfected by pGY577 (40% of total DNA) ...
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bioRxiv - Genetics 2021Quote: ... and viral particles were concentrated using the Lenti-X Concentrator (Takara Bio) and resuspended with McCoy’s 5A (Wisent ...
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bioRxiv - Genomics 2021Quote: ... Viral particles were generated using the Lenti-X Single Shot System (Clontech). 72 hours later ...
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bioRxiv - Cancer Biology 2023Quote: ... Virus particles were precipitated with Lenti-X Concentrator (#631232, Takara Bio, Japan), suspended in phosphate buffer saline (PBS ...
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bioRxiv - Neuroscience 2024Quote: ... AAV2/9 particles were purified using the AAV Purification kit (Takara, Japan). The AAV solution was concentrated to the optimal volume by centrifugation using an Amicon Ultra-4 100k centrifugal filter unit (Millipore) ...
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bioRxiv - Immunology 2023Quote: ... Lentiviral particles were generated by transfection of Lenti-X 293T cells (Takara) with 15 ug LeGO expression vector ...
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bioRxiv - Synthetic Biology 2023Quote: ... lentiviral particles were harvested and concentrated using Lenti-X concentrator (Takara Bio) and stored at -80°C ...
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bioRxiv - Immunology 2022Quote: ... and then the expression construction of CMV-tdTomato was amplified from pCMV-tdTomato vector (Clontech, 632534) to inserted downstream of HA-R by GeneArt Gibson Assembly (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: ... lentiviral particles were concentrated 10-fold using Lenti-X Concentrator (Takara Biosystems 631231).
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bioRxiv - Developmental Biology 2021Quote: ... titration of AAV6 particles was performed using the AAVpro titration kit (TAKARA BIO).
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bioRxiv - Neuroscience 2022Quote: ... Collected lentiviral particles were purified with lenti-X concentrator (Clontech, Mountain View, CA).
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bioRxiv - Molecular Biology 2019Quote: ... Lentivirus particles or VLPs were isolated using a Lenti-X Concentrator (321231, Clontech).
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bioRxiv - Biochemistry 2021Quote: ... viral particles were first generated in HEK-293FT cells using PVLX-puro (Clontech) alongside psPAX2 and pMD2.G obtained from Addgene ...
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bioRxiv - Cell Biology 2021Quote: ... Lentiviral particles were harvested and concentrated using a Lenti-X Concentrator (Clontech; 631231). Concentrated lentiviral particles were supplemented with polybrene (SIGMA ...
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bioRxiv - Microbiology 2022Quote: ... lentiviral particles were prepared using the Lenti-X™ Packaging system (Takara Bio). For the pLV-CMV-IRES-PuroR-T2A-mAmetrine vectors ...
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bioRxiv - Cell Biology 2022Quote: ... Viral particles were precipitated using Lenti-X maxi-purification kit (Takara, Cat# 631233) based on manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Lentiviral particles were concentrated from clarified supernatants using Lenti-X Concentrator (Takara Bio). RAW 264.7 cells were transduced with the lentiviral particles and then selected with 4 μg/mL puromycin (Gibco) ...
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bioRxiv - Molecular Biology 2023Quote: The viral particles were concentrated by precipitation using the Lenti-X concentrator (Clontech) and resuspended in cell culture medium ...
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bioRxiv - Neuroscience 2023Quote: ... AAV2/9 particles were purified using the AAV Purification kit (Takara, Siga, Japan). The AAV solution was concentrated to the optimal volume by centrifugation using an Amicon Ultra-4 100k centrifugal filter unit (Millipore) ...
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bioRxiv - Molecular Biology 2023Quote: ... we enriched the lentiviral particles with Lent-X Concentrator (Cat# 631231, Clontech, Japan) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... we harvested and concentrated the lentiviral particles using the LentiX concentrator (Takara #631232) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... coding regions were cloned downstream of the CMV promoter in derivatives of the pEGFP-N1 plasmid (Clontech) with an NLS and linker sequences to EGFP or mCherry (Supplemental Table 2) ...
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bioRxiv - Plant Biology 2020Quote: For β-galactosidase assay constructs were processed according to the instructions of the β-galactosidase assay kit provided by Clontech. For each construct ...
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bioRxiv - Molecular Biology 2020Quote: ... The empty coding vector pcDNA3.1/MYC-His was obtained from Invitrogen and pCMV-β-Galactosidase was obtained from Clontech/Takara Bio.
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bioRxiv - Cancer Biology 2021Quote: ... The retroviral particles were prepared as mentioned above and concentrated using reterconcentrator (Takara Biosciences). Organoid fragments were prepared following the protocol by (15 ...
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bioRxiv - Biochemistry 2021Quote: ... The presence of lentiviral particles was confirmed using Lenti-X GoStix Plus (631280, Clontech) according to the manufacturer’s instructions.
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... Lentiviral particles were produced by transfecting HEK293T cells (#632180; Clontech, Westburg, Leusden, The Netherlands) according to the manufacturer’s protocol (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: ... Medium containing lentiviral particles was concentrated using a Lenti-X concentrator (Takara Bio, Japan) and resuspended in Neurobasal (ThermoFisher) ...
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bioRxiv - Immunology 2023Quote: ... The viral particles in the supernatant were then concentrated using LentiX Concentrator (Takara Bio), in accordance with the manufacturer’s instructions and resuspended at 10X final concentration in PBS.
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bioRxiv - Molecular Biology 2024Quote: ... viral particles were collected and concentrated using a Lenti-X concentrator (631231; Takara Bio). Lentiviral titers were measured using Lenti-X GoStix (PT5185-2 ...
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bioRxiv - Cell Biology 2022Quote: ... then into pShuttle-CMV with EcoRV using the In-Fusion HD Cloning Plus System kit (Takara Bio USA). These were linearized with Pme1 and cotransformed with pAdEasy-1 into BJ5183 cells ...
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bioRxiv - Molecular Biology 2021Quote: ... a plasmid expressing mCherry under the CMV promoter (pmCherry) was cloned by removing EGFP from pEGFP-C1 (Clontech) with AgeI and BglII and assembling the vector with a synthesized mCherry gene fragment (IDT ...
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bioRxiv - Cell Biology 2022Quote: ... We used IF cloning for the pBI dual expression vector containing a minimal CMV promoter (Takara Bio USA) (Fig ...
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bioRxiv - Biochemistry 2022Quote: ... Normalization was made by measuring the β-Gal activity after 1-hour incubation of 7.5 µl of cellular extract with 50 µl of β-Gal substrate (β-Gal detection kit II, Clontech).
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bioRxiv - Cell Biology 2023Quote: ... The β-galactosidase activity was determined by ONPG (o-nitrophenyl β-D-galactopyranoside) liquid assay (quantitative) as mentioned elsewhere (Clontech Laboratories, 2008). β-galactosidase activity from each sample was calculated from at least three biological replicates and the concentration of cells (O.D.600 ...
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bioRxiv - Molecular Biology 2021Quote: ... which was cleaved with Xba1 and Cla1 and ~2.6 kbp CMV-IRES-puror Xba1+Cla1 fragment from pQCXIP (Clontech) was inserted to construct pHIV-CMV-IRES-puro ...
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bioRxiv - Molecular Biology 2019Quote: ... The fragment was excised from the pMD18-T vector and ligated into the pLVSIN CMV Puro destination vector (TaKaRa) resulting in the pLVSIN-miR-378/486 vector ...