Labshake search
Citations for Takara Bio :
1 - 50 of 776 citations for Adenovirus Type 5 Particles CMV β galatosidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: ... The recombinant adenovirus was produced using an Adenovirus Dual Expression Kit (Takara Bio) in accordance with the manufacturer’s protocol ...
-
bioRxiv - Physiology 2021Quote: ... 0.26 μg of pCMX-PPARγ vector and 0.1 μg of β-galactosidase expression plasmid (CMV-β-Gal) (Clontech, now Takara Bio USA. Inc) vector for normalization.
-
bioRxiv - Physiology 2021Quote: ... 0.26 μg of pCMX-PPARγ vector and 0.1 μg of β-galactosidase expression plasmid (CMV-β-Gal) (Clontech, now Takara Bio USA. Inc) vector for normalization.
-
bioRxiv - Molecular Biology 2023Quote: ... in which the CMV promoter of pAAV-CMV vector (Takara Bio) was replaced by gfaABC1D ...
-
bioRxiv - Microbiology 2020Quote: ... CSII-CMV-MCS-IRES2-Bsd (RIKEN BRC) and pLVSIN-CMV Pur (Takara Bio), respectively ...
-
bioRxiv - Cell Biology 2020Quote: ... Adenovirus expressing human SPARC was constructed using Adeno-X expression system (Clontech) as described before 30,31 ...
-
bioRxiv - Developmental Biology 2020Quote: ... The CMV-MitoDsRed2 (pDsRed2-Mito) and CMV-MitoGFP (pAcGFP1-Mito) plasmids are from Clontech. Atoh7-MitoDsRed2 was designed using UGene software (Unipro) ...
-
bioRxiv - Immunology 2020Quote: ... 1.3 μg CMV 8.91 (Clontech), and 1.5 μg of target vector were transfected using TransIT-293T transfection reagent (Mirus Bio) ...
-
Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... and CCR6high and transduced with lentivirus particles at an MOI of 5 using retronectin (TaKaRa) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... The CMV-GFP construct was pEGFPC1 (Clontech). HIV-1 bearing a Ba-L envelope gene has been described (Rasaiyaah et al. ...
-
bioRxiv - Synthetic Biology 2020Quote: ... The deletion of the lacZ gene was verified using blue-white selection on an LB agar plate containing 0.1 mM isopropyl-β-D-thiogalactopyranoside (Nacalai Tesque) and 4.0 × 10−3 % 5-bromo-4-chloro-3-indolyl-β-D-galactoside (Takara Bio).
-
bioRxiv - Molecular Biology 2020Quote: Adenovirus vectors were generated with the Adeno-X Adenoviral System 3 following manufacturer’s instructions (Takara, #632267). Briefly ...
-
bioRxiv - Molecular Biology 2022Quote: ... The titer of adenovirus was examined using an Adeno-XTM Rapid Titer Kit (Takara Bio USA) and determined to be 3.4 × 1011 ifu/ml ...
-
bioRxiv - Molecular Biology 2022Quote: ... These coding gene sequences were all cloned into CMV-based FLAG-tagging and Clover-tagging vectors (CMV-FLAG-J1 and CMV-Clover-J1; derived from commercially available vectors pEGFP-C1 and pEGFP-N1; Clontech) for ectopic expression in human cells.
-
bioRxiv - Neuroscience 2022Quote: ... Mover-IRES-palmGFP (promoter: CMV; backbone: Clontech N1); mGFP-Mover (promoter ...
-
bioRxiv - Neuroscience 2020Quote: ... the CMV-EGFP plasmid (pEGFP; Clontech, CA, USA) was used as a positive control ...
-
bioRxiv - Synthetic Biology 2023Quote: ... VH and VL segments were ordered as gene blocks from Integrated DNA Technologies and were cloned into linearized CMV/R backbones with 5× In-Fusion HD Enzyme Premix (Takara Bio).
-
bioRxiv - Physiology 2021Quote: ... Adenovirus was generated in HEK293A cells and titered using the Adeno-X Rapid Titer Kit (Takara Bio). For differentiation ...
-
bioRxiv - Cell Biology 2022Quote: ... the CMV promoter from pEGFP-C1 (Takara Bio Inc) was removed and replaced with the SEPTIN2 promoter using AseI and AgeI ...
-
bioRxiv - Cancer Biology 2024Quote: ... pHTN HaloTag® CMV-neo and pHTC HaloTag® CMV-neo were digested with restriction enzymes and subsequently ligated (Takara, cat n°6023). Primers and corresponding restriction digests can be found in Table S4 ...
-
bioRxiv - Immunology 2022Quote: ... Recombinant adenovirus vaccines were produced using the Adeno-X™ Adenoviral System 3 according to the manufacturer’s manual (Takara Korea Biomedical Inc. ...
-
bioRxiv - Microbiology 2022Quote: ... The recombinant adenovirus was prepared in HEK293 cells and purified with an Adeno-X Virus Purification kit (Takara Bio). The purified virus titer was determined using an Adeno-X Rapid Titer kit (Takara Bio) ...
-
bioRxiv - Cell Biology 2021Quote: ... pEGFP and mCherry vectors were from Clontech (with CMV promoter) with either C1 or N1 cloning sites to obtain the tag either at the N-terminus or C-terminus of Arc ...
-
bioRxiv - Immunology 2021Quote: ... wild-type SARS-CoV-2 RBD was purified using a 5 mL HisTALON superflow cartridge (Takara Bio) followed by size exclusion chromatography using a Superdex 200 10/300 GL column pre-equilibrated in 20 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Physiology 2023Quote: ... The adenovirus titer in plaque-forming units (PFU) was determined using a Adeno-X Rapid Titer kit (Clontech, Palo Alto, CA).
-
bioRxiv - Biochemistry 2021Quote: ... The β-galactosidase expression vector pCMV-β (CLONTECH, Palo Alto, CA, USA) was used as an internal control ...
-
bioRxiv - Biophysics 2021Quote: ... viral particles produced by transfecting LentiX-293T (Takara Bio) cells with the sgRNA lentiviral vector were added to the dual stable cell lines ...
-
bioRxiv - Cell Biology 2020Quote: ... Viral particles were precipitated using Retro-X Concentrator (Clontech) and resuspended in STEMdiff Neural Progenitor Medium containing 8 μg/ml Polybrene (EMD Millipore) ...
-
bioRxiv - Cell Biology 2022Quote: ... AAV particles were isolated using AAVPro extraction solution (Clontech). After transduction with both CRISPR/Cas9 gesicles and AAV homology donor ...
-
bioRxiv - Genomics 2021Quote: ... Lentiviral particles were produced in 293T-Lenti-X (Clontech) cells cultured in DMEM ...
-
bioRxiv - Cell Biology 2023Quote: Lentiviral particles were generated using the pLVX system (Clontech) with packaging vectors psPAX2 and pMD2.G (Addgene plasmids #12260 and #12259) ...
-
bioRxiv - Immunology 2022Quote: ... Lentiviral particles were produced in Lenti-X cells (Clontech) per manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... Lentiviral particles were concentrated using the LentiX Concentrator (Clontech). Organoids were trypsinized into small cell clusters and incubated with the concentrated virus particles supplemented with 4 μg/mL Polybrene (Sigma Aldrich) ...
-
bioRxiv - Neuroscience 2023Quote: ... Cousin lab was dropped into an pEGFP-N1 CMV mammalian expression vector (Clontech) by In-fusion cloning ...
-
bioRxiv - Neuroscience 2024Quote: The mammalian expression vector pBApo-CMV-Pur (cat. #3421) was purchased from Takara. We used Genescript to insert the 144 amino acid sequence for WT synuclein into the backbone and create 6 mutated strains ...
-
bioRxiv - Cell Biology 2023Quote: ... procollagen type I (Takara #MK101), fibronectin (Takara #MK115) ...
-
bioRxiv - Cell Biology 2022Quote: ... Lentiviral particles were further concentrated using Lenti-X concentrator (Clontech) following the manufacturer’s instructions.
-
bioRxiv - Biophysics 2019Quote: ... and particles were concentrated using Lenti-X Concentrator reagent (Clontech) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2020Quote: ... Viral particles were packaged in Lenti-X 293T cells (Clontech) with two package plasmids ...
-
bioRxiv - Immunology 2020Quote: ... Lentiviral particles were concentrated using Lenti-X Concentrator (Takara Bio).
-
bioRxiv - Immunology 2022Quote: ... as well as with retroviral particles with RetroNectin® (Takara) following manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... Lentivirus particles were concentrated with Lenti-XTM Concentrator (Takara 631232) with the manufacturers protocol ...
-
bioRxiv - Biochemistry 2023Quote: ... Lentiviral particles were packaged using Lenti-X 293T cells (Clontech) following the instructions ...
-
bioRxiv - Biochemistry 2022Quote: ... Lentivirus particles were concentrated with Lenti-XTM Concentrator (Takara Bio). SH-SY5Y was transduced by lentivirus before differentiation ...
-
bioRxiv - Molecular Biology 2023Quote: ... Lentivirus particles were further concentrated using Lenti-X Concentrator (Clontech) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: Lentiviral particles were produced in Lenti-X 293T cells (Takara). Approximately 10x106 cells were transfected with a mixture of pVSV-G (1.7 µg) ...
-
bioRxiv - Immunology 2020Quote: ... Tcrb amplicons were prepared using a 5’RACE-based protocol with the SMARTer Mouse TCR α/β Profiling Kit (Takara #634402) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2019Quote: ... was first produced by modifying a CMV-driven eGFP cassette from pEGFP-N1 (Clontech), such that the CMV immediate early promoter was partially duplicated with an iscei::kan cassette inserted between the duplicated sequences ...
-
bioRxiv - Microbiology 2023Quote: ... and cynomolgus monkey were cloned into a pLVSIN-CMV Hyg vector (TaKaRa, Cat# 6182) predigested with NotI-HF (NEB ...
-
bioRxiv - Microbiology 2020Quote: ... HIV viral particles were purified using the Lenti-X concentrator (Takara) and were resuspended in fresh DMEM medium supplemented with 5% of FBS ...