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Citations for Takara Bio :
451 - 500 of 798 citations for 7 S 17 S dihydroxy 8 E 10 Z 13 Z 15 E 19 Z Docosapentaenoic Acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: 8 DIV (days in vitro) neurons were transfected with 1.5 µg of pEGFP-N1 vector (Clontech Laboratories, Takara Bio Inc., Mountain View, CA, USA) or pEGFP-N1-TG2 vector (Furini et al. ...
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bioRxiv - Bioengineering 2024Quote: ... To synthesize cDNA, total RNA (8 µL, ca. 500 ng) was mixed with 2 µL of PrimeScript™ RT Master Mix (Takara Bio, Kusatsu, Japan) and incubated at 37°C for 15 min ...
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bioRxiv - Microbiology 2021Quote: ... 10 U ml−1 of HRV- 3C protease (Takara) were added to the Ni-NTA eluted protein to remove the oligohistidine- GST-tag during dialysis against 3 L of dialysis buffer I (25 mM Tris-HCl ...
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bioRxiv - Synthetic Biology 2019Quote: ... 10 nM of the chemical dimerizer AP20187 (ClonTech Laboratories) or left unstimulated ...
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bioRxiv - Microbiology 2019Quote: ... containing 10% Tetracycline-free fetal bovine serum complex (Clontech) and 1% penicillin-streptomycin (100 U penicillin/mL ...
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bioRxiv - Plant Biology 2022Quote: ... 10 μL of SYBR Premix Ex TaqTM II (Takara), and 0.4μL of reference dye ...
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bioRxiv - Neuroscience 2020Quote: ... and 10% FBS (Clontech Laboratories, Mountain View, CA, USA). All cell lines (LN229 (Accession Nr ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 10% certified Tet-system approved FBS (Clontech, 631101). Transfections were performed using the TransIT-X2 system (Mirus ...
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bioRxiv - Genomics 2021Quote: ... 5 μL of 10× ExTaq buffer (Takara Bio Inc.), 5 μL of 2.5 mM dNTPs (Takara Bio Inc.) ...
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bioRxiv - Zoology 2021Quote: ... 10 μL of 2×SYBR Green Premix (Takara, Japan), and 6.8 μL ddH2O ...
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bioRxiv - Microbiology 2021Quote: ... and 10 μL (1 mU) of Pfu PGAP (TaKaRa) was added ...
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bioRxiv - Genomics 2019Quote: ... 0.4-mM dNTP Mix (50X/10 mM, Clontech, 639206), 0.48-μM IS PCR Primer (12 μM ...
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bioRxiv - Microbiology 2020Quote: ... and 10 U μl−1 PrimeScript Reverse Transcriptase (Takara) at 48 °C for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with 10% tetracycline-free FBS (Takara Bio, 631106), 1% Penicillin/Streptomycin and 2μg/ml blasticidin ...
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bioRxiv - Plant Biology 2022Quote: ... 10 μl SYBR Premix Ex Taq II (Takara, Japan), and nuclease-free water ...
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bioRxiv - Molecular Biology 2022Quote: ... and concentrated 10-fold using Lenti-X concentrator (Takara). Lentivirus aliquots were stored at -80 °C until functional titration and use ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 0.8 µL of 10 mM dNTPs (Takara #4030), with the following thermal cycle condition ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 0.8 µL of 10 mM dNTPs (Takara #4030), with the following thermal cycle condition ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 0.8 µL of 10 mM dNTPs (Takara #4030), with the following thermal cycle condition ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 0.64 µL of 10 mM dNTPs (Takara #4030), with the following thermal cycle condition ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 0.8 µL of 10 mM dNTPs (Takara #4030), with the following thermal cycle condition ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 0.8 µL of 10 mM dNTPs (Takara #4030), with the following thermal cycle condition ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 0.4 µL of 10 mM dNTPs (Takara #4030), with the following thermal cycle condition ...
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bioRxiv - Synthetic Biology 2023Quote: ... with 10% Tet Approved FBS (Clontech Laboratories or Avantor) and 1X Penicillin/Streptomycin (Life Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... and concentrated 10-fold using Lenti-X concentrator (Takara). Lentivirus aliquots were stored at -80 °C until functional titration and use.
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bioRxiv - Genetics 2022Quote: ... on RetroNectin-coated plates (10 μg/cm2, Takara Bio) for at least 2 h ...
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bioRxiv - Genetics 2022Quote: ... on RetroNectin-coated plates (10 μg/cm2, Takara Bio). Cells were then transduced in the same medium ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% Tet system-approved FBS (Takara #631368), 50 µg/mL hygromycin B ...
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bioRxiv - Plant Biology 2024Quote: ... 10 µL SYBR Premix Ex Taq II (Takara, China), 1 µL (200 nM final concentration ...
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bioRxiv - Immunology 2024Quote: ... and 10 μl Premix Ex Taq (TaKaRa, Shiga, Japan). Amplification conditions were as follows ...
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bioRxiv - Molecular Biology 2023Quote: ... medium supplemented with 10% Fetal bovine serum (FBS, Clontech). One well from a 6-well-plate at 70% confluency was used for the transfection ...
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bioRxiv - Cancer Biology 2019Quote: ... 3 μg of total RNA was reverse transcribed to cDNA with Oligo(dT)15 primers using PrimeScript™ Reverse Transcriptase (Takara Bio, Shiga, Japan). The resultant cDNA was subjected to real-time PCR by using SYBR® Premix Ex Taq™ II (Takara Bio ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Each cDNA sample was amplified for the gene of interest and β-actin in a 15 µL reaction volume TB GreenTM Premix Ex Taq™ II (Takara, Tokyo, Japan). The PCR conditions were 95 °C for 30 s followed by 40 cycles of 95 °C for 5 s and 60 °C for 60 s ...
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bioRxiv - Cell Biology 2023Quote: ... Expression level of Venus and Venus-tagged arrestin-3 proteins was determined with anti-GFP JL-8 antibody (#632381, Takara Bio USA, San Jose, CA). The endogenous β-actin (loading control ...
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bioRxiv - Biophysics 2019Quote: ... The pCD86-EGFP had been created by inserting CD86 with a 21 amino acid linker coding region on its C-terminus immediately before EGFP in pEGFP-N1 (Clontech/Takara, Mountain View, CA). The CD86-21aa was amplified from pCD86-mEos2 (Mike Heilemann via Addgene ...
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bioRxiv - Cell Biology 2019Quote: Amino acid substitutions in RNF167 were made using site-directed mutagenesis by Prime Star GXL-DNA polymerase (R050A, Takara Bio Inc., Otsu, Japan) according to the manufacturer’s protocol with the following primers:
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bioRxiv - Microbiology 2021Quote: ... and BGH PCR products had 15 bp of overlapping sequence with adjacent inserts and backbone and were assembled with In-Fusion (Takara Bio, Mountain View, CA, USA) to create the final construct ...
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bioRxiv - Cell Biology 2020Quote: ... Protein samples were run on a 4-12% Bis-Tris gel (Novex) and Western blots were performed with the following antibodies: α-GFP (Clontech Living Colors 632381 (JL-8); RRID:AB_2313808 ...
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bioRxiv - Biochemistry 2020Quote: ... supplemented with 10% (v/v) Tet-system approved FBS (Clontech), 100 U/mL penicillin ...
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bioRxiv - Neuroscience 2020Quote: ... supplemented with 10 % Tet system approved FBS (Takara Bio USA) and 1% antibiotic ...
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bioRxiv - Microbiology 2020Quote: ... the GFP1-10 ORF was amplified from pEGFP-C1 (Clontech) and Gibson-assembled into NsiI and PacI digested pGRA-HA-HPT (a gift from Moritz Treeck ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10% Tet system approved FBS (Takara Bio USA), 1mM sodium pyruvate ...
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bioRxiv - Cell Biology 2019Quote: ... 10 mM imidazole) and 40 µL of TALON beads (Clontech) was added ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were then resuspended in Neurobasal with 10 % FBS (Clontech), mEGF (100 ng/mL ...
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bioRxiv - Biochemistry 2021Quote: ... After loading onto a 5ml or 10 ml Talon (Clontech) or Ni-NTA (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... 10 μL of 2× One Step RT-PCR buffer (TaKaRa), and 5 μL ddH2O ...
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bioRxiv - Cell Biology 2022Quote: ... with 10% fetal bovine serum (tetracycline-free FBS, Takara Bio) and 10 U mL-1 penicillin and 10 µg mL-1 streptomycin (Pen-Strep ...
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bioRxiv - Neuroscience 2023Quote: ... A 10 μl In-Fusion Cloning reaction (Takara-bio Inc.) was prepared by adding 2 μl of 5X In-Fusion HD Enzyme Premix ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR mixture containing 10 µL Emerald Master mix (2X) (TaKaRa), 2 µL total genomic DNA (10-15 ng/µL) ...
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bioRxiv - Molecular Biology 2023Quote: ... which was supplemented with 10% Tet System Approved FBS (TaKaRa), penicillin/streptomycin ...