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Citations for Takara Bio :
4851 - 4900 of 6263 citations for Triiodothyronine T3 ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... The cells were grown for 72 hours post-transfection and the virus was concentrated ∼40-fold from the supernatant using the Lenti-X concentrator kit (Takara) per the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... esrp1 and esrp2 were each cloned into a pCS2+8 plasmid backbone using the In-Fusion HD Cloning Kit (Clontech). The resulting pCS2+8-esrp2 plasmid was mutagenized with synonymous mutations surrounding the translational start-site using the GeneArt site-directed mutagenesis (SDM ...
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bioRxiv - Bioengineering 2024Quote: ... The purified PCR product was cloned with a C-terminus 6-His tag into pET22b vector via NdeI/XhoI using the In-Fusion HD Cloning kit following the manufacturer’s protocol (Takara Bio). The construct was transformed into chemically competent E ...
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bioRxiv - Molecular Biology 2024Quote: ... and the insert fragments fused to the linearized plasmids using the In-Fusion Cloning Kit (Takara Bio Inc., Shiga, Japan), according to the protocol provided by the manufacturer with the Escherichia coli JM109 strain ...
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bioRxiv - Developmental Biology 2024Quote: ... Sample tubes were reverse transcribed and amplified using the SMART-Seq V4 Ultra Low Input RNA Kit (Takara Bio 634891) in 10x reduced volume reactions using a Mantis liquid handling system (Lee et al ...
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bioRxiv - Plant Biology 2024Quote: ... First-strand cDNA was synthesized from RNA (approximately 2 μg) with oligo(dT) primers according to the instructions of the PrimeScript First Strand cDNA Synthesis Kit (Takara).
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bioRxiv - Microbiology 2024Quote: ... The quantified 400 ng of RNA used for cDNA preparation using the PrimeScript 1st strand cDNA Synthesis Kit (Takara Bio). The forward and reverse primers used for amplification are used as previously described (32) ...
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bioRxiv - Microbiology 2024Quote: ... Then the total RNA was extracted and reversed transcribed into cDNA for quantitative PCR by using the PrimeScript RT Reagent Kit (TaKaRa). Quantitative real-time reverse transcriptase PCR (qRT-PCR ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA synthesis and library preparations were performed by the Genomics Core using the SMARTv4 for ultra-low RNA input and Nextera XT kits (Takara). Sequencing was also performed on an Illumina NextSeq 2000 sequencing system with paired-end ...
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bioRxiv - Immunology 2024Quote: ... total RNA (0.5 ng) was added to reaction buffer from the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara), and reverse transcription was performed followed by PCR amplification to generate full length amplified cDNA ...
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bioRxiv - Genomics 2024Quote: ... 10 ng of total RNA was amplified and converted into cDNA using SMART-Seq v4 Ultra Low Input RNA kit (Clontech). About 17 fmol of cDNA was used for library preparation using the PCR Barcoding kit (SQK-PBK004 kit ...
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bioRxiv - Systems Biology 2024Quote: ... in conjunction with the Mir-X™ miRNA qRT-PCR TB Green® Kit from TAKARA (San Jose, CA, USA). After confirming miRNA inhibition in tick tissues ...
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bioRxiv - Plant Biology 2020Quote: Total RNA was extracted from plant samples with Purelink (Nacalai Tesque, Japan) and reverse transcribed with PrimeScript Reagent Kit Perfect Real Time (Takara, Japan) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... The yeast one-hybrid (Y1H) assay was conducted using the Matchmaker™ Gold Yeast One-Hybrid Library Screening System kit (Cat. no. 630491, Clontech).
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bioRxiv - Plant Biology 2019Quote: ... One microgram of DNA-free RNA was used to synthesize cDNA by using PrimeScriptTM RT Reagent Kit (TAKARA, Kusatsu, Shiga, Japan).
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bioRxiv - Plant Biology 2019Quote: ... 1 μg DNase I-treated RNA was used for synthesizing the first strand cDNA according to the manufacturer’s instructions (PrimeScript II 1st Strand cDNA Synthesis Kit; Takara, Dalian, China). The primers of EcaICE1 and EcaSIZ1 (listed as table 1 ...
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bioRxiv - Biophysics 2021Quote: ... RNA was synthetized using the T7-Scribe transcription kit (Cellscript) and purified with Chroma Spin DEPC-H2O columns (Clontech, CA, USA). The ncAA naphthalene was ligated to the tRNA with T4 DNA ligase (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... HSP70-F2/R2 primers (Table S1) and ligated into pBS-SK+ using the In-fusion HD Cloning kit (Clontech, Beijing, China), respectively ...
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bioRxiv - Cancer Biology 2021Quote: ... and quantitative real-time PCR was performed using the SYBR® Premix Ex Taq II Reagent Kit (Takara, Cat. Cat. #RR820A) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... PCR amplified fragments and linearized vector by restriction enzyme digestion were assembled using In-Fusion HD Cloning Kit (Takara Bio Inc.). pEGFPN3-ARL4C was constructed as previously described(Matsumoto et al ...
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bioRxiv - Cell Biology 2020Quote: ... 10ng of each RNA isolate was then prepared using the Takara SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian (Takara, 634412). Transcripts were fragmented for 3.5 minutes and amplified for 12 cycles ...
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bioRxiv - Immunology 2021Quote: ... DNase-treated total RNA was used to prepare cDNA libraries with the SMARTer smRNA-Seq Kit (Takara Bio, Mountain View, CA). Libraries were sequenced as 50 bp single-end reads on an Illumina HiSeq sequencer ...
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bioRxiv - Genetics 2021Quote: Adult ovary total RNA was used to amplify the 5’ and 3’ UTR of Pxmeiw68 and PxnanosP using the SMARTer RACE 5’/3’ Kit (Takara, Japan). 5’ and 3’ regulatory sequences were then cloned from 4th instar larval gDNA ...
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bioRxiv - Developmental Biology 2021Quote: ... three gene-specific reverse primers were designed and a nested PCR program was used according to the protocol of a genome walking kit (Takara, Japan). The sequences of the primers used for amplification are shown in Table S1 ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR donor amplicons were purified using the Nucleospin Gel and PCR Clean-up kit (Machery-Nagel, distributed by Takara Bio USA). PCR products were not gel purified ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and was converted into cDNA prior to sequencing using the SMARTer technology (SMARTer Pico PCR cDNA Synthesis Kit, Takara, cat. # 634928). Sequencing libraries were prepared using the Illumina TruSeq protocol and sequenced on an Illumina MiSeq using 250 bp paired end reads ...
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Three Distinct Transcriptional Profiles of Monocytes Associate with Disease Activity in SSc PatientsbioRxiv - Genomics 2022Quote: ... Full-length RNA-seq libraries from CM and skin macrophages were prepared from ∼3 ng of total RNA using SMART-Seq v4 Ultra Low Input RNA Kit (Clontech Laboratories) followed by Nextera XT protocol (Illumina) ...
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bioRxiv - Molecular Biology 2022Quote: ... The amplified fragments were purified using a gel extraction kit (Vazyme, China) and cloned into a pMD-18T vector (Takara, Japan).
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bioRxiv - Cell Biology 2022Quote: ... The gene encoding TERT and mVenus was inserted into pSBbi vector using the In-Fusion cloning kit (Takara Bio, Shiga, Japan). The R3E/R6E mutation was introduced using the KOD mutagenesis kit (Toyobo ...
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bioRxiv - Molecular Biology 2020Quote: ... the genome of Pv11 cells and the clonal cell lines were extracted with a NucleoSpin Tissue kit (Takara Bio, Shiga, Japan) and subjected to PCR using the following primer set ...
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bioRxiv - Molecular Biology 2020Quote: ... Single cell polyA[+] RNA-seq on the C1 was performed using the SMART-Seq v4 Ultra Low Input RNA Kit for the Fluidigm C1 System (Takara, 635026) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: cDNA synthesis and amplification was performed using a modified protocol for the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Bio). Briefly ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and each RNA concentration was measured by quantitative PCR after reverse transcription using PrimeScript One Step RT-PCR Kit (TaKaRa, Japan) with specific primers (Table S2) ...
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bioRxiv - Genomics 2020Quote: ... RNA-seq library preparation was performed using the SMARTer Stranded Total RNA-Seq Kit v2 - Pico (Takara Bio, Mountain View, CA) prior to 100 bp paired-end sequencing ...
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bioRxiv - Genomics 2020Quote: ... Full-length cDNAs were generated on the Fluidigm C1 system using the SMART-Seq v4 Ultra Low Input RNA kit (Clontech, 635026) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... Transcripts rising from the identified TSS were determined using the SMARTer Rapid amplification of cDNA ends (RACE) 5’/3’ kit (Takara Bio) in accordance with the manufacturer’s instructions for 3’ RACE ...
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bioRxiv - Genomics 2020Quote: ... and the resulting DNase I-treated RNA (~2 ng) was processed for sequencing by using a SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio) according to the manufacturer’s protocol with 12 cycles of PCR followed by two rounds of clean up with 1.3X Agencourt AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Molecular Biology 2021Quote: ... Gene expression in the cDNA samples was measured by one-step qRT-PCR using a One Step PrimeScript RT-PCR Kit (Perfect Real Time) according to the manufacturer’s specifications (Takara, Dalian, China). Quantitative real-time PCR was performed on the CFX Connect Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Pathology 2019Quote: ... The first-strand cDNA was synthesized from 1μg total RNA using an oligo dT primer according to the protocol of PrimeScript™RT reagent Kit with gDNA Eraser (TAKARA). The expression level of PDS of cucumber ...
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bioRxiv - Biochemistry 2019Quote: ... RT was performed using Oligo(dT) random 6-mer primers from a Prime Script RT Master Mix kit (TaKaRa, Dalian, China) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... Total RNA was then extracted from macrophages using the TRIzol method and converted into cDNA by PrimeScript™ RT-PCR Kit (Takara). The following genes were amplified ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: RNA was extracted from the granulation tissue of the rats using a NucleoSpin® RNA II kit (Takara Bio, Otsu, Japan). Each sample was harvested from the right quarter of a wound with approximately 5-mm margins without the cranial bone ...
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bioRxiv - Biophysics 2020Quote: ... coli MG1655 genome by colony PCR and cloned into pR6K-PAmCherry-lox71-cm-lox66 using the In-Fusion® HD cloning kit (Clontech). Mutations were introduced using the QuikChange II Site-Directed Mutagenesis Kit (Agilent) ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR products were inserted into pLEX-FLAG lentiviral vector between the NotI and XhoI sites using the InFusion HD cloning kit (Takara Bio) to generate constructs with N-terminal FLAG tags ...
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bioRxiv - Cell Biology 2019Quote: ... LW2A and YKLW4A were cloned from plasmid pECerulean (37) to pHH21 with In-Fusion® HD Cloning Kit (Takara Bio, Japan). Recombinant Influenza viruses were produced with the twelve plasmids system (38 ...
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bioRxiv - Cell Biology 2019Quote: ... and PrimeScript RT reagent kit with gDNA Eraser and SYBR Premix Ex Taq II (T1i RNase H Plus) (TaKaRa, Otsu, Japan) were utilized to carry out quantitative RT-PCR (qRT-PCR ...
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bioRxiv - Developmental Biology 2019Quote: ... 5’ and 3’ RACE was carried out according to the manufacturer’s protocol of SMART RACE cDNA Amplification kit (Clontech, Takara, Japan). The sequences of primers for RACE are as follows ...
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bioRxiv - Cell Biology 2019Quote: ... was extracted and 5’ and 3’ rapid amplification of cDNA ends (RACE) was performed using the Smarter RACE kit (Clontech, 634858). cDNA was synthesized as described in the kit using 5′ and 3′ RACE CDS primers and SMARTer IIA oligo for template switching for 5′ RACE ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR products were gel purified (Machery Nagel) and used to generate α-32P dCTP-labeled probes using the Random Prime Labeling Kit (Takara/Clontech). Probes were purified over nucleotide purification columns (Zymo Research ...
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bioRxiv - Immunology 2019Quote: ... was added to each sample and first strand full length cDNA was generated with a modified protocol of the SMARTseq v4 Ultra Low Input RNA Kit (Takara Clontech) using poly dT primers and a template switching oligo ...