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Citations for Takara Bio :
4551 - 4600 of 5557 citations for Human E3 Ubiquitin Protein Ligase SMURF2 SMURF2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... The resulting double-stranded cDNA’s were further processed to DNA sequencing libraries using ThruPLEX DNA-seq 12S Kit (R400428, Clontech Laboratories). Libraries were size-selected by gel purification for an average size of 350bp ...
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bioRxiv - Genetics 2023Quote: ... Reverse transcription was performed using the oligo (dT) primer and AMV reverse transcriptase contained in the TaKaRa RNA PCR kit (TaKaRa). The RT-PCR was performed using KOD One PCR Master Mix (TOYOBO ...
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bioRxiv - Microbiology 2023Quote: ... The resulting amplicons were cloned into pKTS786 (a pcDNA 3.1/V5-His based vector) that had been linearized with BstX1 with an In-Fusion HD cloning kit (Takara 638909) (76) ...
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bioRxiv - Molecular Biology 2023Quote: ... These bulk kidney tissues were process from an OCT block using the SMARTer Stranded Total RNA-Seq Kit v2 (Takara). Sequencing was performed in a 2×75 bp paired-end configuration using a NovaSeq platform (Illumina).
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bioRxiv - Molecular Biology 2023Quote: ... generated cDNA was subjected to real-time PCR analysis using SYBR® Premix Ex Taq™ II kit (TAKARA BIO) with the sets of specific primers (Table 1) ...
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bioRxiv - Cancer Biology 2023Quote: ... The number of virus copies/ml was determined using the Lenti-X™ qRT-PCR Titration Kit (Takara Bioscience, 631235) according to the manual ...
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bioRxiv - Genomics 2023Quote: ... double-stranded DNA library libraries from the size fractionated samples were constructed using the ThruPLEX DNA-seq Kit (Takara Bio) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: The full-length SKP1-FBXO22 fusion protein with a 3xGGGS-linker was cloned into a pNic-Bio2 vector that encodes for an N-terminal His-tag as well as a C-terminal Avi-tag (GenBank: JF912191) using an In-Fusion HD Cloning System kit (Takara), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... above 20% were used for downstream library generation using the SMARTer Stranded Total RNA – Pico RNA-seq kit v2 (Takara). Libraries were sequenced using 100 nucleotide paired-end sequencing on a NovaSeq 6000 (Illumina).
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bioRxiv - Immunology 2023Quote: ... A cDNA library for TCR was prepared from RNA using a SMARTer Mouse TCR a/b Profiling Kit (Takara Bio) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... reverse transcription and cDNA pre-amplification were performed using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech/Takara). cDNA was harvested and quantified with the Bioanalyzer DNA High-Sensitivity kit (Agilent Technologies) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Reverse transcription (RT) reactions were performed with 1 μg total RNA using the PrimeScript RT kit with gDNA Eraser (Takara). The resulting cDNA was amplified in triplicate on the 480 Real-Time PCR system (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... and were reverse transcribed to first-strand cDNAs with random primers using PrimeScript First Strand cDNA Synthesis Kit (Takara, Japan). Quantitative PCR (qPCR ...
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bioRxiv - Immunology 2023Quote: ... Each gene fragment was PCR-amplified and cloned into a pcDNA 3.1 vector by using the In-Fusion HD cloning kit (Takara Bio). Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... The viral RNA copy number was standardized using a SARS-CoV-2 direct detection RT-qPCR kit (Takara, Cat# RC300A). Fluorescent signals from resulting PCR products were acquired using a Thermal Cycler Dice Real Time System III (Takara).
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bioRxiv - Cell Biology 2023Quote: ... The DNA fragment coding GFP was inserted at the 5’ side of SYP32 by In-Fusion HD Cloning Kit (Takara), and the whole sequence was recombined into pGWB1 by LR Clonase II ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA-seq libraries from input and IP samples were prepared using the SMARTer Pico Input Mammalian v2 RNA-seq kit (Takara) and sequenced as SE50 runs on an Illumina HiSeq4000.
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bioRxiv - Immunology 2023Quote: ... cells were resuspended in Trizol reagent and frozen at -20°C until RNA was extracted using the Macherey-Nagel Nucleospin RNA XS Kit (CAS: 740902.50, Takara Bio) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: Full-length HSF1 and its sequences were obtained by the rapid amplification of cDNA ends using a SMART cDNA amplification kit (Clontech). Gene-specific primers (GSPs ...
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bioRxiv - Cell Biology 2023Quote: Each single-cell RNA-seq library was generated from a single FGO or a single MII oocyte using SMART-Seq HT Kit (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: RNA was prepared and cDNA was synthesized with the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara) and ran the Agilent Tapestation to assess cDNA product ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product was run on a 1% agarose gel at 85 V for 35 min and gel extracted using the Nucleospin Gel Extraction Kit (Takara). We then used PCR with KOD Polymerase 2x Mastermix (Millipore Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 μg of total RNA was reverse transcribed into complementary DNA (cDNA) using PrimeScript™ RT Reagent Kit (Takara, RR047B). Amplification of cDNA product was performed using specific primers with the TB Green® Premix Ex Taq™ II (Takara ...
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bioRxiv - Microbiology 2023Quote: ... This (DNA-free) RNA was reverse transcribed into cDNA by using PrimeScript RT reagent Kit with gDNA Eraser (Takara, Japan). Quantitative PCR was performed using a LightCycler 480 (Roche ...
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bioRxiv - Microbiology 2023Quote: ... These three amplified fragments were then seamlessly integrated into the Sac I site of YEP352-GAPII (Oka et al. 2007) using the In-Fusion HD Cloning Kit (Takara). Similarly ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were collected and AAV6 capsids were isolated using the AAVPro Purification Kit (All Serotypes, Takara Bio, San Jose, USA), as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... 4F2hc and mZIP13 or mZIP14 were fused by PCR and In-Fusion® HD Cloning Kit (Takara Bio, Shiga, Japan), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... amounts ranging from 10 picograms to 10 nanograms of total RNA were employed using the SMART-seq v4 Ultra Low Input RNA Kit from Takara Bio ...
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bioRxiv - Genetics 2024Quote: ... Esrp1 cDNA was cloned into the pcDNA3.1 backbone containing a CMV promoter and SV40 polyA tailing sequence for expression in mammalian cells using the In-Fusion HD Cloning Kit (Clontech) to generate the pcDNA3.1-esrp1-mCherry plasmid ...
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bioRxiv - Developmental Biology 2024Quote: ... quantitative real-time PCR (RT-qPCR) was performed using the cDNA libraries as the template and a TB Green Premix Ex Taq kit (Takara) in a CFX96 real-time PCR machine (BioRad) ...
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CTCF regulates global chromatin accessibility and transcription during rod photoreceptor developmentbioRxiv - Genomics 2024Quote: ... and 1 ng total RNA was used to generate RNA-seq libraries with SMARTer Stranded Total RNA-Seq Kit v3 (Takara). All samples were sequenced with NovaSeq 6000 (Illumina ...
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bioRxiv - Developmental Biology 2024Quote: ... then we reversed transcription of these RNA to create cDNA by a PrimeScript 1st strand cDNA synthesis kit (Takara, Japan). FMNL2-EGFP vector was generated by Wuhan GeneCreate Biological Engineering Co ...
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bioRxiv - Cell Biology 2024Quote: ... the RNA was reverse transcribed into complementary DNA by PrimeScript RT (reverse transcriptase) using a primeScript RT-PCR kit (Takara), and qPCR was performed with SYBR Green-Master Mix (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... gene-specific primers adding a His-tag sequence were used with the In-Fusion® HD EcoDry™ kit (Takara). The complementation construct containing A ...
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bioRxiv - Synthetic Biology 2024Quote: ... Ribo-seq RNA libraries were prepared for sequencing using the SMARTer® smRNA-Seq Kit for Illumina (#635030) from Takara Bio USA ...
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bioRxiv - Genomics 2024Quote: ... Alu6B and Aluh1.1 were synthesized as gene blocks (IDT) and cloned into BamHI-AatII sites in the pYa5-neo vector using Infusion cloning kit (Takara) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... was inserted into pcDNA5-FRT/TO to generate pcDNA/TO/Dyn1-K44A-GFP using the In-Fusion cloning kit (Takara), and the following primers ...
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bioRxiv - Genetics 2024Quote: ... ∼0.37 pg) per tube—was subjected to amplification using a PicoPLEX® Gold Single Cell DNA-Seq Kit (Takara Bio) and indexing primers (DNA HT Dual Index kit ...
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bioRxiv - Immunology 2024Quote: ... 100ng of total RNA was used for cDNA synthesis by RNA to cDNA EcoDry™ Premix (Double Primed) kit (Takara), and qPCR was performed by Taqman primers with TaqMan™ Fast Advanced Master Mix (Applied Biosystems™ ...
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bioRxiv - Plant Biology 2024Quote: ... respectively) were inserted into the KpnI and XbaI sites of the vector using the In-Fusion HD Cloning Kit (TaKaRa).
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bioRxiv - Bioengineering 2024Quote: ... The rituximab and cetuximab antibody variable sequences were generated by IDT and cloned into the variable regions of the VRC01 antibody plasmid vector (a generous gift from the Kim lab at Stanford) by using the In-Fusion cloning kit (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... AAV titer was determined by qPCR method using AAVpro Titration Kit (for Real Time PCR) Ver.2 (Takara-bio, #6233) following the manufacturer’s protocol.
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bioRxiv - Cell Biology 2024Quote: cfDNA in the conditioned cell culture medium was extracted using NucleoSpin Gel and PCR Clean-up kit (Takara, Duren, Germany), and quantified with Nanodrop (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were grown for 72 hours post-transfection and the virus was concentrated ∼40-fold from the supernatant using the Lenti-X concentrator kit (Takara) per the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... esrp1 and esrp2 were each cloned into a pCS2+8 plasmid backbone using the In-Fusion HD Cloning Kit (Clontech). The resulting pCS2+8-esrp2 plasmid was mutagenized with synonymous mutations surrounding the translational start-site using the GeneArt site-directed mutagenesis (SDM ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Libraries were harvested by scraping colonies from transformation plates and directly purifying plasmids (with no outgrowth that could introduce amplification bias) using Nucleobond Xtra midiprep kits (Takara).
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bioRxiv - Bioengineering 2024Quote: ... The purified PCR product was cloned with a C-terminus 6-His tag into pET22b vector via NdeI/XhoI using the In-Fusion HD Cloning kit following the manufacturer’s protocol (Takara Bio). The construct was transformed into chemically competent E ...
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bioRxiv - Molecular Biology 2024Quote: ... and the insert fragments fused to the linearized plasmids using the In-Fusion Cloning Kit (Takara Bio Inc., Shiga, Japan), according to the protocol provided by the manufacturer with the Escherichia coli JM109 strain ...
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bioRxiv - Developmental Biology 2024Quote: ... Sample tubes were reverse transcribed and amplified using the SMART-Seq V4 Ultra Low Input RNA Kit (Takara Bio 634891) in 10x reduced volume reactions using a Mantis liquid handling system (Lee et al ...
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bioRxiv - Plant Biology 2024Quote: ... First-strand cDNA was synthesized from RNA (approximately 2 μg) with oligo(dT) primers according to the instructions of the PrimeScript First Strand cDNA Synthesis Kit (Takara).