Labshake search
Citations for Takara Bio :
4501 - 4550 of 5557 citations for Human E3 Ubiquitin Protein Ligase SMURF2 SMURF2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... Total RNA was then used for library preparation using the SMARTer small RNA (smRNA)-seq kit for Illumina (Takara Bio) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... and cloning it into the EcoRI and EcoRV sites of pcDNA-EGFP-P2A in frame with the tag sequence by using an In-Fusion® HD Cloning Kit according to the manufacturer’s instructions (Takara). pcDNA-hAPOBEC1 was constructed by cloning the XhoI-HindIII fragments of pUC57-APOBEC1 ...
-
bioRxiv - Molecular Biology 2023Quote: ... DNA encoding HsPINK1 was inserted into the BamHI site of pcDNA5/FRT/TO CMVd3 vector (pcDNA5d3, see (17)) using the In-Fusion HD Cloning Kit (Takara). For stable expression ...
-
bioRxiv - Molecular Biology 2023Quote: ... the 3’ UTR of the virus were determined using the SMARTer®RACE 5’/3’ Kit (Takara Bio, Kusatsu, Japan). 2.4 µg of RGMoV gRNA was polyadenylated using Poly(A)polymerase (600 u/µl ...
-
bioRxiv - Cell Biology 2023Quote: ... The cDNAs were cloned into the pmCherryC1 vector using an In-Fusion HD cloning kit (Clontech, Mountain View, CA, USA).
-
bioRxiv - Cancer Biology 2023Quote: ... the agarose gel pieces were cut out and digested using NucleoSpin® Gel and PCR Clean-Up kit (Takara, 740609), according to the manufacturer’s protocols ...
-
bioRxiv - Biochemistry 2023Quote: TssM193-490 coding region was obtained by gene synthesis (IDT) and cloned into pOPIN-K vector45 using the In-Fusion HD Cloning Kit (Takara Clontech). Point mutations were introduced using the QuikChange Lightning kit (Agilent Technologies).
-
bioRxiv - Molecular Biology 2023Quote: ... the supernatants of infected cells were collected and infectious virus particles were measured using the Adeno X Rapid Titration Kit (Takara Bio Europe ...
-
bioRxiv - Molecular Biology 2023Quote: ... the RNA was subjected to reverse transcription to generate RNA cDNA using the widely recognized PrimeScriptRT kit (Takara, Kyoto, Japan) provided by the esteemed Bio-Rad ...
-
bioRxiv - Molecular Biology 2023Quote: ... The PCR products were purified and ligated into the pMD-20 vector or the pANT vector using the Mighty TA- cloning Kit (Takara) or TA-Enhancer Cloning Kit (Nippon Gene ...
-
bioRxiv - Neuroscience 2023Quote: ... cDNA was synthesized from 10 ng of total RNA using SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio), according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... was inserted in between the KpnI and HindIII sites of the pOPINK vector (42) using the In-Fusion HD Cloning Kit (Takara). The pOPINK vector incorporates an N-terminal GST tag and a 3C protease cleavage site into the PhPINK1 construct ...
-
bioRxiv - Developmental Biology 2023Quote: ... The yeast two-hybrid library was generated from the poly(A)+ mRNA isolated from mixed-stage N2 nematodes using the Matchmaker kit (Clontech) as per the manufacturer’s protocol and transformed into Y1HGold strain (MATα ...
-
bioRxiv - Immunology 2023Quote: ... covering SIV env and nef were amplified from plasma viral RNA by nested RT-PCR using Prime-Script one-step RT-PCR kit v2 (TaKaRa) and KOD-Plus v2 (Toyobo) ...
-
bioRxiv - Cancer Biology 2023Quote: ... forward-Trail and reverse-Trail were generated in PZH vector amplified and extracted using In-Fusion HD Cloning kit (Clontech). Plasmids were sent for Sanger sequencing with reverse and forward primer to control any mutation point ...
-
bioRxiv - Immunology 2023Quote: ... Complementary DNA was prepared using a SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech Laboratories, CA, USA). Libraries were prepared using a Nextera XT DNA Library Prep Kit (Illumine ...
-
bioRxiv - Genomics 2023Quote: ... 1 μl of 5’ linker (10 μM) was ligated with 10 μl of Mighty mix (DNA Ligation Kit
, catalog num. 6023, Takara) to 4 μl of the sample coming from the previous step ... -
bioRxiv - Pharmacology and Toxicology 2023Quote: ... PrimeScript RT reagent kit with gDNA Eraser and TB Green® Premix Ex TaqTM were purchased from Takara (Otsu, Japan). Molecular Probes™ Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry ...
-
bioRxiv - Immunology 2023Quote: The SMARTSeq HT Ultra Low Input Kit was used for full-length cDNA synthesis and amplification (Clontech, Mountain View, CA), and Illumina Nextera XT library was used for sequencing library preparation ...
-
bioRxiv - Microbiology 2023Quote: ... The viral RNA copy number was standardized with a SARS-CoV-2 direct detection RT-qPCR kit (Takara, Cat# RC300A). Fluorescent signals were acquired using a QuantStudio 1 Real-Time PCR system (Thermo Fisher Scientific) ...
-
bioRxiv - Neuroscience 2023Quote: ... the RNA concentration was determined via Nanodrop and up to 100 ng was used as an input material for library preparation using the Clontech Ultra Low kit (Clontech).
-
bioRxiv - Genomics 2023Quote: ... 10 ng of total RNA were amplified and converted to cDNA using SMART-Seq v4 Ultra Low Input RNA kit (Clontech). Afterwards an average of 10 fmol of amplified cDNA was used to prepare library following SQK-PBK004 kit (PCR Barcoding kit ...
-
bioRxiv - Immunology 2023Quote: ... RNA sequencing libraries were prepared by Edinburgh Genomics using a SMART-Seq® v4 PLUS RNA Kit (Takara Bio USA) with 9 cycles of amplification ...
-
bioRxiv - Biochemistry 2023Quote: ... Unique clones were sub-cloned into the sAB expression vector pRH2.2 (kind gift of S. Sidhu) using the In-Fusion Cloning Kit (Takara Bio). The best candidates (based on highest ELISA signals ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... A total of 500 ng of sheared DNAs was input for methyl-CpG binding domain (MBD) enrichment using the EpiXplore Methylated DNA Enrichment Kit (Clontech) according to the manufacturer’s instruction ...
-
bioRxiv - Systems Biology 2023Quote: ... RNA-seq cDNA libraries were prepared using the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (Takara, #634485), including rRNA depletion during library construction ...
-
bioRxiv - Microbiology 2022Quote: ... was used as a template to generate the pCMV-myc-SFPQ ΔRRM1-2 by mutagenesis using the InFusion HD kit from Clontech (catalog number 639649 ...
-
bioRxiv - Genomics 2022Quote: RNA sequencing were prepared using the SMARTer® Stranded Total RNA-Seq Kit v3 -Pico Input Mammalian (Takara cat# 634485) with 5 µl RNA from either the total RNA or the ElementZero eluate as starting material according to the manufacturer’s protocoll ...
-
bioRxiv - Immunology 2023Quote: 150-200 tuft cells were sorted directly into lysis buffer from the SMART-Seq v4 Ultra Low Input RNA Kit (Takara) and cDNA generated following manufacturer’s instructions ...
-
bioRxiv - Genetics 2023Quote: Total RNA extracted from freshly harvested spikes at single ridge end-stage with the RNAprep pure Plant Kit (Biofit Biotechnologies co. Ltd, Chengdu, China) was digested with RNase-free DNase (Takara) to remove residual genomic DNA ...
-
bioRxiv - Genetics 2023Quote: ... cDNA was generated from 2 ng of RNA using the Smart-Sq V4 Ultra Low Input RNA Kit (Takara, 634894). Next ...
-
bioRxiv - Cell Biology 2023Quote: ... Reverse transcription (RT) reactions were performed with 1 μg total RNA using the PrimeScript RT kit with gDNA Eraser (Takara). The resulting cDNA was amplified in triplicate on the 480 Real-Time PCR system (Roche ...
-
bioRxiv - Cell Biology 2023Quote: ... plus any amplicon smaller than the 9246 bp WT band for the large indel screen were gel purified with the NucleoSpin Gel clean up kit (Takara Bio ...
-
bioRxiv - Developmental Biology 2023Quote: ... and RT-PCR was performed to detect sorcs2 mRNA in wt embryos using the Titanium One-Step RT-PCR kit (Clontech) with primers 5’-TTTTGCGCACCTGTACCCAGCTG-3’ and 5’-TAACGCGCTCCTGAAGCAGAGTC-3’ for detection of mRNA after injection of different amounts of sMO and 5’-TTTTGCGCACCTGTACCCAGCTGTGTT-3’ and 5’ TAACGCGCTCCTGAAGCAGAGTCCATT-3’ for the different developmental stages ...
-
bioRxiv - Microbiology 2023Quote: ... genomic DNA using the indicated primers (Table S1).Fluorescent reporters were ordered as gBlocks and cloned using the In-fusion HD EcoDry Cloning kit (Takara). Promoter reporter constructs were created as previously described (11 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Where possible equivalent amounts of total RNA (100pg) from all samples were used for first strand synthesis with the SmartERv3 kit (Takara Clontech) followed by 15-18 cycles of amplification (according to manufacturers’ instruction) ...
-
bioRxiv - Physiology 2023Quote: ... 1 ng of total RNA was used to amplify the cDNA using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech-Takara) following manufactureŕs instructions ...
-
bioRxiv - Immunology 2023Quote: ... Total RNA was concentrated by evaporation using an Eppendorf Vacufuge system and was then added to lysis buffer from the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara). Reverse transcription was performed followed by 20 cycles of PCR amplification to generate full length amplified cDNA ...
-
bioRxiv - Cancer Biology 2023Quote: ... The RNA-seq libraries were prepared using a SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (Takara Bio) and sequenced on an Illumina NovaSeq 6000 using a NovaSeq S4 flow cell with 100 bp paired end reads by MedGenome ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... A total of six libraries were generated using the ThruPLEX DNA-seq 6S (12) kit (Takara Bio Europe, TOWN, France). The concentration of each sample pool was measured with Quant-iT PicoGreen dsDNA assay kit (Invitrogen Life Technologies ...
-
bioRxiv - Genetics 2023Quote: ... and subjected to quantitative real-time polymerase chain reaction with a TB Green Premix Ex Taq II kit (Takara, RR820A). The PCR program consisted of 95°C for 1 minute ...
-
bioRxiv - Immunology 2023Quote: ... RNA-seq libraries were generated from 5 ng of RNA with the SMART-Seq v4 Ultra Low Input RNA Kit from Takara Bio (#634889 ...
-
bioRxiv - Immunology 2023Quote: ... Opposing strand-specific RNA-seq libraries were generated using the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (Takara), according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... and sequencing libraries prepared from 10ng RNA using the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... The RNA was quantified and 1.5 μg of RNA was used to prepare cDNA using PrimeScript 1st strand cDNA Synthesis Kit (Takara Bio) and random hexamer primer ...
-
bioRxiv - Cancer Biology 2023Quote: ... The H2afx coding sequence from Mus musculus was ordered from Eurofins and cloned into the pOZ-N-FH backbone adding the 1xHA tag at the N terminus using the in-fusion HD cloning kit (#12141, Takara). Full length wild type H2afx coding sequence was then mutagenized to obtain the desired S139A point mutation.
-
bioRxiv - Microbiology 2023Quote: ... The sequencing library was prepared using the SMART-Seq v4 Ultra Low Input RNA Kit with nonstranded and polyA+ selection (Clontech, Takara Bio USA ...
-
bioRxiv - Plant Biology 2023Quote: ... and cDNA was synthesized with 2 μg RNA using PrimeScript™ 1st strand cDNA synthesis kit (TaKaRa, Kusatsu, Shiga, Japan) in a final reaction volume of 20 μl following the manufacturer’s protocol.
-
bioRxiv - Neuroscience 2023Quote: ... cDNA was synthesized with the PrimeScript 1st Strand kit with an additional primer mix containing random DTs (#RR047A and #6110A, Takara). cDNAs were amplified using specific Taqman probes ...
-
bioRxiv - Developmental Biology 2024Quote: ... cDNA encoding ZP3 was restored from the mouse ovarian tissue by RT-PCR using PrimeScript RT Reagent Kit (Takara, RR037). Two PCR amplicons including the 5′ region of the TECTA-ZP and the transmembrane domain (TMD ...