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Citations for Takara Bio :
4551 - 4600 of 5541 citations for Galactose Colorimetric Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: 150-200 tuft cells were sorted directly into lysis buffer from the SMART-Seq v4 Ultra Low Input RNA Kit (Takara) and cDNA generated following manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: Total RNA extracted from freshly harvested spikes at single ridge end-stage with the RNAprep pure Plant Kit (Biofit Biotechnologies co. Ltd, Chengdu, China) was digested with RNase-free DNase (Takara) to remove residual genomic DNA ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription (RT) reactions were performed with 1 μg total RNA using the PrimeScript RT kit with gDNA Eraser (Takara). The resulting cDNA was amplified in triplicate on the 480 Real-Time PCR system (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... plus any amplicon smaller than the 9246 bp WT band for the large indel screen were gel purified with the NucleoSpin Gel clean up kit (Takara Bio ...
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bioRxiv - Developmental Biology 2023Quote: ... and RT-PCR was performed to detect sorcs2 mRNA in wt embryos using the Titanium One-Step RT-PCR kit (Clontech) with primers 5’-TTTTGCGCACCTGTACCCAGCTG-3’ and 5’-TAACGCGCTCCTGAAGCAGAGTC-3’ for detection of mRNA after injection of different amounts of sMO and 5’-TTTTGCGCACCTGTACCCAGCTGTGTT-3’ and 5’ TAACGCGCTCCTGAAGCAGAGTCCATT-3’ for the different developmental stages ...
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bioRxiv - Microbiology 2023Quote: ... genomic DNA using the indicated primers (Table S1).Fluorescent reporters were ordered as gBlocks and cloned using the In-fusion HD EcoDry Cloning kit (Takara). Promoter reporter constructs were created as previously described (11 ...
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bioRxiv - Cancer Biology 2023Quote: ... Where possible equivalent amounts of total RNA (100pg) from all samples were used for first strand synthesis with the SmartERv3 kit (Takara Clontech) followed by 15-18 cycles of amplification (according to manufacturers’ instruction) ...
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bioRxiv - Physiology 2023Quote: ... 1 ng of total RNA was used to amplify the cDNA using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech-Takara) following manufactureŕs instructions ...
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bioRxiv - Immunology 2023Quote: ... Total RNA was concentrated by evaporation using an Eppendorf Vacufuge system and was then added to lysis buffer from the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara). Reverse transcription was performed followed by 20 cycles of PCR amplification to generate full length amplified cDNA ...
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bioRxiv - Cancer Biology 2023Quote: ... The RNA-seq libraries were prepared using a SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (Takara Bio) and sequenced on an Illumina NovaSeq 6000 using a NovaSeq S4 flow cell with 100 bp paired end reads by MedGenome ...
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bioRxiv - Evolutionary Biology 2023Quote: ... A total of six libraries were generated using the ThruPLEX DNA-seq 6S (12) kit (Takara Bio Europe, TOWN, France). The concentration of each sample pool was measured with Quant-iT PicoGreen dsDNA assay kit (Invitrogen Life Technologies ...
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bioRxiv - Genetics 2023Quote: ... and subjected to quantitative real-time polymerase chain reaction with a TB Green Premix Ex Taq II kit (Takara, RR820A). The PCR program consisted of 95°C for 1 minute ...
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bioRxiv - Immunology 2023Quote: ... RNA-seq libraries were generated from 5 ng of RNA with the SMART-Seq v4 Ultra Low Input RNA Kit from Takara Bio (#634889 ...
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bioRxiv - Immunology 2023Quote: ... Opposing strand-specific RNA-seq libraries were generated using the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (Takara), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and sequencing libraries prepared from 10ng RNA using the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The RNA was quantified and 1.5 μg of RNA was used to prepare cDNA using PrimeScript 1st strand cDNA Synthesis Kit (Takara Bio) and random hexamer primer ...
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bioRxiv - Cancer Biology 2023Quote: ... The H2afx coding sequence from Mus musculus was ordered from Eurofins and cloned into the pOZ-N-FH backbone adding the 1xHA tag at the N terminus using the in-fusion HD cloning kit (#12141, Takara). Full length wild type H2afx coding sequence was then mutagenized to obtain the desired S139A point mutation.
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bioRxiv - Microbiology 2023Quote: ... The sequencing library was prepared using the SMART-Seq v4 Ultra Low Input RNA Kit with nonstranded and polyA+ selection (Clontech, Takara Bio USA ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA was synthesized with the PrimeScript 1st Strand kit with an additional primer mix containing random DTs (#RR047A and #6110A, Takara). cDNAs were amplified using specific Taqman probes ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA encoding ZP3 was restored from the mouse ovarian tissue by RT-PCR using PrimeScript RT Reagent Kit (Takara, RR037). Two PCR amplicons including the 5′ region of the TECTA-ZP and the transmembrane domain (TMD ...
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bioRxiv - Developmental Biology 2024Quote: ... The resulting double-stranded cDNA’s were further processed to DNA sequencing libraries using ThruPLEX DNA-seq 12S Kit (R400428, Clontech Laboratories). Libraries were size-selected by gel purification for an average size of 350bp ...
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bioRxiv - Genetics 2023Quote: ... Reverse transcription was performed using the oligo (dT) primer and AMV reverse transcriptase contained in the TaKaRa RNA PCR kit (TaKaRa). The RT-PCR was performed using KOD One PCR Master Mix (TOYOBO ...
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bioRxiv - Microbiology 2023Quote: ... The resulting amplicons were cloned into pKTS786 (a pcDNA 3.1/V5-His based vector) that had been linearized with BstX1 with an In-Fusion HD cloning kit (Takara 638909) (76) ...
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bioRxiv - Molecular Biology 2023Quote: ... These bulk kidney tissues were process from an OCT block using the SMARTer Stranded Total RNA-Seq Kit v2 (Takara). Sequencing was performed in a 2×75 bp paired-end configuration using a NovaSeq platform (Illumina).
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bioRxiv - Molecular Biology 2023Quote: ... generated cDNA was subjected to real-time PCR analysis using SYBR® Premix Ex Taq™ II kit (TAKARA BIO) with the sets of specific primers (Table 1) ...
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bioRxiv - Cancer Biology 2023Quote: ... The number of virus copies/ml was determined using the Lenti-X™ qRT-PCR Titration Kit (Takara Bioscience, 631235) according to the manual ...
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bioRxiv - Genomics 2023Quote: ... double-stranded DNA library libraries from the size fractionated samples were constructed using the ThruPLEX DNA-seq Kit (Takara Bio) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: The full-length SKP1-FBXO22 fusion protein with a 3xGGGS-linker was cloned into a pNic-Bio2 vector that encodes for an N-terminal His-tag as well as a C-terminal Avi-tag (GenBank: JF912191) using an In-Fusion HD Cloning System kit (Takara), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... above 20% were used for downstream library generation using the SMARTer Stranded Total RNA – Pico RNA-seq kit v2 (Takara). Libraries were sequenced using 100 nucleotide paired-end sequencing on a NovaSeq 6000 (Illumina).
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bioRxiv - Immunology 2023Quote: ... A cDNA library for TCR was prepared from RNA using a SMARTer Mouse TCR a/b Profiling Kit (Takara Bio) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... reverse transcription and cDNA pre-amplification were performed using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech/Takara). cDNA was harvested and quantified with the Bioanalyzer DNA High-Sensitivity kit (Agilent Technologies) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Reverse transcription (RT) reactions were performed with 1 μg total RNA using the PrimeScript RT kit with gDNA Eraser (Takara). The resulting cDNA was amplified in triplicate on the 480 Real-Time PCR system (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... and were reverse transcribed to first-strand cDNAs with random primers using PrimeScript First Strand cDNA Synthesis Kit (Takara, Japan). Quantitative PCR (qPCR ...
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bioRxiv - Immunology 2023Quote: ... cDNA libraries were generated using SMARTer Human TCR a/b Profiling Kit v2 (Takara Bio USA, San Jose, California, USA). Briefly ...
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bioRxiv - Immunology 2023Quote: ... Each gene fragment was PCR-amplified and cloned into a pcDNA 3.1 vector by using the In-Fusion HD cloning kit (Takara Bio). Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... The DNA fragment coding GFP was inserted at the 5’ side of SYP32 by In-Fusion HD Cloning Kit (Takara), and the whole sequence was recombined into pGWB1 by LR Clonase II ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA-seq libraries from input and IP samples were prepared using the SMARTer Pico Input Mammalian v2 RNA-seq kit (Takara) and sequenced as SE50 runs on an Illumina HiSeq4000.
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bioRxiv - Immunology 2023Quote: ... cells were resuspended in Trizol reagent and frozen at -20°C until RNA was extracted using the Macherey-Nagel Nucleospin RNA XS Kit (CAS: 740902.50, Takara Bio) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: Full-length HSF1 and its sequences were obtained by the rapid amplification of cDNA ends using a SMART cDNA amplification kit (Clontech). Gene-specific primers (GSPs ...
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bioRxiv - Cell Biology 2023Quote: Each single-cell RNA-seq library was generated from a single FGO or a single MII oocyte using SMART-Seq HT Kit (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: RNA was prepared and cDNA was synthesized with the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara) and ran the Agilent Tapestation to assess cDNA product ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product was run on a 1% agarose gel at 85 V for 35 min and gel extracted using the Nucleospin Gel Extraction Kit (Takara). We then used PCR with KOD Polymerase 2x Mastermix (Millipore Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 μg of total RNA was reverse transcribed into complementary DNA (cDNA) using PrimeScript™ RT Reagent Kit (Takara, RR047B). Amplification of cDNA product was performed using specific primers with the TB Green® Premix Ex Taq™ II (Takara ...
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bioRxiv - Microbiology 2023Quote: ... This (DNA-free) RNA was reverse transcribed into cDNA by using PrimeScript RT reagent Kit with gDNA Eraser (Takara, Japan). Quantitative PCR was performed using a LightCycler 480 (Roche ...
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bioRxiv - Microbiology 2023Quote: ... These three amplified fragments were then seamlessly integrated into the Sac I site of YEP352-GAPII (Oka et al. 2007) using the In-Fusion HD Cloning Kit (Takara). Similarly ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were collected and AAV6 capsids were isolated using the AAVPro Purification Kit (All Serotypes, Takara Bio, San Jose, USA), as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... 4F2hc and mZIP13 or mZIP14 were fused by PCR and In-Fusion® HD Cloning Kit (Takara Bio, Shiga, Japan), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... amounts ranging from 10 picograms to 10 nanograms of total RNA were employed using the SMART-seq v4 Ultra Low Input RNA Kit from Takara Bio ...
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bioRxiv - Genetics 2024Quote: ... Esrp1 cDNA was cloned into the pcDNA3.1 backbone containing a CMV promoter and SV40 polyA tailing sequence for expression in mammalian cells using the In-Fusion HD Cloning Kit (Clontech) to generate the pcDNA3.1-esrp1-mCherry plasmid ...
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bioRxiv - Developmental Biology 2024Quote: ... quantitative real-time PCR (RT-qPCR) was performed using the cDNA libraries as the template and a TB Green Premix Ex Taq kit (Takara) in a CFX96 real-time PCR machine (BioRad) ...