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Citations for Takara Bio :
401 - 450 of 4968 citations for Rat Myristoylated Alanine Rich C Kinase Substrate MARCKS ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... The first strand of cDNA was synthesized by reverse transcription of the mRNA via a 1hr incubation at 42°C by adding the following to the reaction mixture above: 1µl SMARTScribe™ RT (Takara, #639538), 1µl dNTPs 10mM (NEB ...
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bioRxiv - Biochemistry 2020Quote: ... HeLa cells stably expressing mKeima-P2A-FRB-Fis1 and FKBP-GFP-ATG13 and mutants were treated with A/C heterodimerizer (for simplicity, it is called “rapalog” in the figure) (Clontech#635056) for 24 h and then subjected to FACS analysis as previously described (Vargas et al. ...
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bioRxiv - Cell Biology 2022Quote: ... Cell lysates were centrifuged (1h, 4°C, 14000 xg) and the soluble fraction was passed through 1mL of Talon Metal Affinity Resin (Clontech #635509). The resin was washed for four times with 4mL Wash Buffer (50mM Tris pH7.5 ...
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bioRxiv - Neuroscience 2022Quote: ... protein lysate was immunoprecipitated for 2 h at 4°C with the following antibodies: mouse anti-GFP (1:1000; Takara Bio), mouse anti-FLAG (1:1000 ...
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bioRxiv - Immunology 2020Quote: ... for 24 h and transduced with lentiviral particles by spinfection (1000 x g for 90 min at 32°C) in the presence of Polybrene (5 μg/ml) on the plates coated with Retronectin (50 μg/ml) (Takara/Clontech) and anti-CD3 (1–2 μg/ml) ...
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bioRxiv - Neuroscience 2019Quote: ... and then in primary antibodies in PBST at 4°C for 48 hours using rabbit anti-DsRed (mCherry tag; 1:500; Clontech; 632496), and mouse anti-Th (1:1,000 ...
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bioRxiv - Cell Biology 2020Quote: ... was added to the StrePD beads and incubated at 37 °C for 10 minutes followed by an incubation with Proteinase K (Takara, #9034) at 60 °C overnight ...
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bioRxiv - Biophysics 2020Quote: ... Transformed yeast cells were cultured at 30°C in uracil dropout synthetic defined medium (-Ura dropout supplement was purchased from Takara Bio). To induce expression of genes under the control of the GAL1 promoter ...
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bioRxiv - Developmental Biology 2022Quote: ... Co-IPs were carried out by incubating the samples with 30 μL of protein A agarose bead slurry for 4h at 4°C in a rotating wheel and with anti-mCherry (Takara 632496) of 1:1000 dilution ...
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bioRxiv - Molecular Biology 2022Quote: ... the transfection medium was replaced with 2 ml of reduced serum culture medium (5% FCS) supplemented with 300 μM A/C heterodimerization agent (formerly AP21967, Takara BioInc), 20 mM HEPES and 10 μM cholesterol (balanced with methyl-β-cyclodextrin ...
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bioRxiv - Genomics 2022Quote: The expression of each erbb3b gene and the two housekeeping genes (rpl7 and rpl32) was obtained by RT-qPCR at 65 °C (PrimeScript transcriptase, Takara, SYBRgreen) and normalized with the Pfaffle equation (Ståhlberg et al. ...
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bioRxiv - Genomics 2022Quote: ... The ligation reactions were heat-inactivated at 65°C for 10 minutes and cleaned up with NucleoMag NGS Clean-up and Size Select beads (Takara Bio) before electroporation into Endura Electrocompetent E ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were centrifuged at 16,000 RCF for 5 min at 4°C and the supernatant was reserved for DNA clean-up using NucleoSpin® Gel and PCR Clean-Up (740609, Takara) based on manufacture instructions or ethanol precipitation.
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bioRxiv - Neuroscience 2023Quote: ... The non-solubilized fraction was separated by centrifugation at 47850 x g for 1 h at 4 °C and 4 mL of cobalt-charged TALON resin (Clontech, Takara) was added to the solubilized fraction for batch binding (3 h at 4 °C) ...
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bioRxiv - Bioengineering 2023Quote: ... Cerulean and Myo7b blots were stained for 1 h at room temperature (RT) or overnight at 4 °C using the mouse anti-GFP (detects cerulean, 1:2000, JL-8, Clontech, Takara) and the mouse anti-β-tubulin antibody (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... in PBS and then incubated overnight with gentle agitation at 4 °C in blocking solution plus 1:1000 dilution primary antibody (chicken anti-GFP polyclonal, Abcam; rabbit anti-dsRed polyclonal, Takara Bio). Sections were then incubated covered with gentle agitation for 2 hours at room temperature in blocking solution plus 1:500 dilution secondary antibody (goat anti-rabbit IgG Alexafluor 594 conjugate ...
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bioRxiv - Immunology 2023Quote: ... Retroviral supernatants were then collected and spun at 2,000g for 2 h at 32 °C onto 24-well non-tissue-culture-treated plates coated overnight in Retronectin (Takara Bio) or spinfected for 90 minutes at 32°C in 1 µcg mL-1 polybrene ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was then diluted 1:5 to improve binding efficiency and incubated overnight on the rotisserie at 4°C with TALON Cobalt Resin (Takara Bio). The next day the resin was washed with 10 column volumes of Membrane Buffer 1 ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was then diluted 1:4 to improve binding efficiency and incubated overnight on the rotisserie at 4°C with TALON Cobalt Resin (Takara Bio). The next day the resin was washed with 10 column volumes of Membrane Buffer 2 and 10 column volumes of Membrane Buffer 2 with 20 mM imidazole before elution with Membrane Buffer 2 with 300 mM imidazole ...
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bioRxiv - Neuroscience 2023Quote: ... lysates were centrifuged at 31,000 x g for 45 min at 4°C and the supernatant was mixed with nickel-nitrilotriacetic acid (Ni-NTA) beads (Takara, 635653) at 4°C for 2 h ...
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bioRxiv - Genomics 2022Quote: ... Paris) were prepared following the kit manufacturer’s recommendations (SMART-Seq v4 Ultra Low Input RNA Kit from Takara). Samples were sequenced on a NextSeq 500 Illumina device with MidOutPut cartridge to generate a total of 2 × 87 M reads of 75 bp ...
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bioRxiv - Genetics 2021Quote: ... The qPCR procedure was carried out according to the kit instructions (TB Green Premix Ex Taq kit, TakaRa). Primers used were as follows:
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bioRxiv - Immunology 2020Quote: ... and reverse transcribed using a first-strand cDNA synthesis kit (PrimeScript™ 1st strand cDNA Synthesis Kit, Takara) according to the manufacturer’s protocol ...
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... Protein concentrations were assessed with the BCA protein assay kit (Takara BCA Protein Assay Kit, Takara, Shiga, Japan). SDS-PAGE and Western blotting were performed as previously described (Shintani et al. ...
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bioRxiv - Bioengineering 2022Quote: ... prepared with a SMARTer Stranded Total RNA-Seq Kit v3 – Pico Input Mammalian kit (Takara Bio Inc., Japan) with 0.75 ng and bulk-sequenced using 100 bp paired-end reads at a depth of 50 million reads ...
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bioRxiv - Genomics 2022Quote: ... Libraries were prepared using the SMARTer® Stranded Total RNA-seq Kit v2-Pico Input Mammalian kit (Takara). Two technical replicates were generated for each experiment ...
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bioRxiv - Microbiology 2022Quote: ... Complementary DNA (cDNA) was synthesized using the PrimeScript RT reagent kit (Perfect Real Time Kit, Takara Biochemicals, China). According to the vendor’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Libraries were constructed with the SMARTer® Stranded Total RNA-Seq Kit – Pico Input Mammalian kit (Clontech Laboratories), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: Sample libraries were prepared with SMART-Seq v4 Ultra Low Input RNA Kit (Takara Clontech Kit Cat# 63488). Libraries were sequenced on a NovaSeq 6000 (Illumina ...
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bioRxiv - Developmental Biology 2023Quote: Sample libraries were prepared with SMART-Seq v4 Ultra Low Input RNA Kit (Takara Clontech Kit Cat# 63488). Libraries were sequenced on a NovaSeq 6000 (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA-seq libraries were prepared using the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian kit (Takara) due to retirement of the ScriptSeq v2 kit ...
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bioRxiv - Cell Biology 2024Quote: ... Complementary DNA (cDNA) was synthesized using the PrimeScript™ RT reagent Kit with gDNA Eraser Kit (TaKaRa, Japan) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... The SMARTer Pico RNA V2 Kit (Clontech) was used for library preparation ...
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bioRxiv - Biochemistry 2021Quote: ... Reverse transcription kit was purchased from TaKaRa, Japan.SYBR Green PCR kit was purchased from American Applied Biosystems ...
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bioRxiv - Immunology 2021Quote: ... using the Reverse Transcriptase Kit (Takara, Japan). Quantitative PCR reactions were carried out in duplicate ...
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bioRxiv - Neuroscience 2022Quote: ... Using the In Fusion Cloning Kit (Clontech) the 5,000 bp amplicon carrying the mutation was inserted into the full-length Reelin-coding plasmid digested with SnaBI and AgeI.
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bioRxiv - Plant Biology 2020Quote: ... using a commercial kit (TaKaRa, Shiga, Japan). Before applying to the root segments ...
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bioRxiv - Cancer Biology 2019Quote: ... purified with NucleoBond Xtra Midi kit (Clontech), and sequenced (Genewiz).
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bioRxiv - Immunology 2019Quote: LDH Cytotoxicity Detection kit (MK401, Takara Bio) was used as per manufacturer instructions to detect cell killing.
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bioRxiv - Molecular Biology 2020Quote: ... Premix Taq version 2.0 kit (TaKaRa, Japan) was used for PCR reaction ...
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bioRxiv - Developmental Biology 2019Quote: ... The Advantage GC 2 PCR Kit (Takara) was used for gene cloning ...
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bioRxiv - Physiology 2019Quote: ... the NucleoSpin RNA XS kit (Takara Bio) was used ...
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bioRxiv - Bioengineering 2019Quote: ... using the Advantage 2 Polymerase kit (Clontech). Complementary 60 bp-ends to the target sequence on M ...
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bioRxiv - Bioengineering 2019Quote: ... using the Advantage 2 Polymerase kit (Clontech) and specific primers located on either side of the target locus ...
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bioRxiv - Genomics 2021Quote: ... SMART-Seq RNA Kit (v4) (Takara, 634891), TruSeq Library Prep (Illumina ...
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bioRxiv - Genomics 2021Quote: ... SMART-Seq Single Cell Kit (Takara Bio) libraries were prepared following the manufacturer instructions ...
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bioRxiv - Microbiology 2021Quote: ... The PrimeScript cDNA synthesis kits (Takara Bio) were used to convert 1 μg of total RNAs into cDNA according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... using the In-Fusion HD kit (Takara), followed by transformations into MegaX DH10B T1R electrocompetent cells (ThermoFisher Scientific) ...
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bioRxiv - Plant Biology 2020Quote: ... Takara Prime Script™ RT kits (Takara Bio Inc. ...
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bioRxiv - Biophysics 2021Quote: ... using In-Fusion HD Cloning Kit (Clontech). BP84 is a chimeric protein of 1-83 amino acid sequence of Bacillus ...