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Citations for Takara Bio :
651 - 700 of 4968 citations for Rat Myristoylated Alanine Rich C Kinase Substrate MARCKS ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... In-Fusion HD Cloning Kit (Takara Bio USA) was used to insert the AVR-Mgk1 fragment (Arg25-Trp85 ...
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bioRxiv - Molecular Biology 2022Quote: ... TB Green Premix Ex TaqTM Ⅱ Kit (TaKaRa) was applied in determining the abundance of mRNA according to the manufacturer’s directions ...
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bioRxiv - Microbiology 2023Quote: ... and Mighty Mix DNA ligation kit (Takara Bio) were used for PCR and DNA ligation reactions ...
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bioRxiv - Neuroscience 2022Quote: ... A SMART-Seq® HT Kit (TAKARA Bio) was used to prepare the amplified cDNA templates following the manufacturer’s instructions (Uchigashima ...
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bioRxiv - Neuroscience 2023Quote: ... reverse transcribed by PrimeScript RT reagent Kit (Takara) and quantified using SYBR Green Real-Time PCR Kit (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... Infusion Cloning HD Kit was procured from Takara Bio USA Inc ...
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bioRxiv - Microbiology 2022Quote: ... The SYBR Premix Ex Taq II kit (TaKaRa) was used for qRT-PCR ...
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bioRxiv - Immunology 2023Quote: ... using the In-Fusion HD cloning kit (Clontech). To make SPPL3 and B3GNT2 double knockout cells ...
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bioRxiv - Immunology 2023Quote: ... and reverse transcribed (PrimeScript RT reagent kit, TaKaRa). Real-time qPCR was performed with the QuantStudio™ 6 Pro Real-Time PCR System (ThermoFisher ...
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bioRxiv - Developmental Biology 2023Quote: ... or SMARTer™ RACE cDNA Amplification Kit (Clontech). A cDNA library was used as a template for PCR (Nanos1 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... PrimeScript RT-PCR Kit (Takara Bio, Shiga, Japan)
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bioRxiv - Developmental Biology 2023Quote: ... the TaKaRa DNA Ligation Kit LONG (TaKaRa Bio) was used along with NEB Stable Competent E ...
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bioRxiv - Genomics 2023Quote: ... coli using the Nucleobond XtraBAC kit (Takara 740436) and preps were stored at 4 °C.
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bioRxiv - Biophysics 2023Quote: ... or In-Fusion HD cloning kit (Takara Bio). The following restriction enzymes were used for cloning ...
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bioRxiv - Cell Biology 2023Quote: ... using the In-Fusion HD cloning kit (Clontech), according to manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... Then One-step PrimeScript RT-PCR kit (Takara) was used to generate cDNA ...
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bioRxiv - Microbiology 2023Quote: ... and ligated with a DNA ligation kit (Takara). The constructs expressing GFP-BDI-bK6A and single amino acid substitutions in the SLiM sequence were generated by PCR-based site-directed mutagenesis using oligonucleotides and templates listed in Table S1 and inserted into the SalI site of pHE-C or phsp-GFP-Halo as described above ...
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bioRxiv - Bioengineering 2023Quote: ... Using a BCA protein kit (TaKaRa, Shiga, Japan), a working solution (BCA reagent A ...
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bioRxiv - Biochemistry 2023Quote: ... using the In-Fusion HD Cloning kit (Takara) to generate NanoLuc fusion on the N-terminus of NSD2 ...
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Bni5 tethers myosin-II to septins to enhance retrograde actin flow and the robustness of cytokinesisbioRxiv - Cell Biology 2023Quote: ... using in-fusion cloning kit (Takara, Kusatsu, Japan). To generate pFA6a-link-GBP-CaURA3 ...
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bioRxiv - Genetics 2023Quote: ... and DNA Unique Dual Index Kit (Takara, R400666). Paired-end sequencing of ChIP-seq libraries was performed (150 bp ...
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bioRxiv - Microbiology 2023Quote: ... Protoplasts were stained following ApoAlert kit indications (TaKaRa) and the ratio of apoptotic cells was measured by flow cytometry ...
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bioRxiv - Cancer Biology 2023Quote: ... employing the CalPhos mammalian transfection kit (Takara Bio). Supernatants were harvested at 48 and 72 h post-transfection and subsequently stored at -80°C ...
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bioRxiv - Genomics 2024Quote: ... we repurposed the SMARTer smRNA-seq kit (Takara). First ...
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bioRxiv - Immunology 2023Quote: ... Kit Advantage 2 PCR Enzyme System (Clontech, 639206), QIAquick Gel Extraction ...
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bioRxiv - Molecular Biology 2024Quote: ... using a random primer DNA labeling kit (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... using the In-Fusion HD Cloning Kit (Clontech) kit ...
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bioRxiv - Immunology 2023Quote: ... using the In-Fusion HD cloning kit (Takara Bio ...
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bioRxiv - Microbiology 2023Quote: ... using the In-Fusion HD Cloning Kit (Takara), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... using the SMARTer smRNA kit for Illumina (Takara), with few modifications ...
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bioRxiv - Microbiology 2024Quote: ... using the In-Fusion HD cloning kit (Clontech). Primers used were OCN334 and OCN430 (ComM) ...
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bioRxiv - Molecular Biology 2023Quote: ... the PrimeScriptTM RT Reagent Kit (Takara Bio Europe) was used ...
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bioRxiv - Cancer Biology 2024Quote: ... the RNA Isolation Kit (RNAiso Plus, Takara, Japan) was employed ...
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bioRxiv - Genomics 2024Quote: SMART-Seq Single Cell PLUS kit (Takara Bio) was used for cDNA library construction ...
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bioRxiv - Microbiology 2020Quote: For Bioluminescence Resonance Energy Transfer (BRET) assay we used the pEYFP-C1/N1 plasmids encompassing EYFP tag in the N- or C-terminal positions (Clontech, Mountain View, CA). To obtain pNluc-C1/N1 plasmids ...
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bioRxiv - Cell Biology 2019Quote: ... for 30 min at 37°C in a moisture chamber followed by an incubation with anti-GFP (1:100 in PBSA) antibody at 4°C for 72 h (Takara 632381/JL-8)) ...
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bioRxiv - Biophysics 2019Quote: ... The pCD86-EGFP had been created by inserting CD86 with a 21 amino acid linker coding region on its C-terminus immediately before EGFP in pEGFP-N1 (Clontech/Takara, Mountain View, CA). The CD86-21aa was amplified from pCD86-mEos2 (Mike Heilemann via Addgene ...
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bioRxiv - Neuroscience 2021Quote: ... All opsins were fused to mRuby2 at their C-terminus and sub-cloned into the pCAGGS expression vector by In-Fusion cloning (Clontech, Mountain View, CA). In order to target the opsins to the soma and proximal dendrites of neurons ...
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bioRxiv - Cancer Biology 2021Quote: All tissues from mice were quick-frozen in liquid nitrogen and stored at −80°C until they were dissolved with Trizol (TaKaRa, Guangzhou, China, A161050A). RNA extraction was performed according to the manufacturer’s instruction ...
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bioRxiv - Physiology 2019Quote: ... Membranes were incubated at 4°C in primary antibodies diluted 1:1000 in 5% bovine serum albumin: anti-GFP (ClonTech Living Colours #ab632375), anti-HSP60 (Department of Biology ...
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bioRxiv - Cell Biology 2020Quote: ... for 1 h at 4 °C with rotation and then transferred to gravity columns (Talon® 2 ml Disposable Gravity Column, Clontech, #635606-CLI). The lysate was drained and the beads were washed five times with cold wash buffer (50 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... Synthesized gene fragments went through cloning process after overnight digestion with restriction enzymes (Nde I, Xho I) at 37 °C and ligation (T4 DNA Ligase, Takara Bio, Shiga, Japan) into pET21 vector (Novagen ...
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bioRxiv - Biochemistry 2021Quote: ... After sonication and centrifugation (20 000 g, 1 h, 4 °C) the supernatant was mixed with Talon Metal Affinity Resin (Takara Bio USA, Inc.). After 1 hour incubation at 4 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... Each sample was then incubated at 37°C for 30 min with 20 U of mRNA-Interferase™ MazF enzyme (Takara, Cat# 2415A) in a 10 μL volume reaction containing 1X MazF reaction buffer ...
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bioRxiv - Cell Biology 2023Quote: We used an hESC line that transiently expresses the C-terminal region (catalytic domain) of histone demethylase JMJD3 in a doxycycline (DOX, Takara Bio Inc, Japan) inducible manner (12 ...
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bioRxiv - Microbiology 2023Quote: ... and was then incubated with 2 mL TBS pH 8.0 pre-equilibrated Talon® Metal Affinity resin during 2 h at 4°C with permanent mixing (Takara Bio, Kusatsu, Japan). The resin was washed with 10 volumes of TBS pH 8.0 buffer containing 0.1% Triton X-100 and 10 mM imidazol (both from Sigma) ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5 μg of total RNAs were reverse-transcribed into cDNA at 42°C for 1 h in 20 μl reaction mixture containing mouse Moloney leukemia virus reverse transcriptase (PrimeScript, TAKARA BIO, Shiga, Japan) with random 6 primers (TAKARA BIO) ...
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bioRxiv - Cell Biology 2024Quote: ... A bicistronic construct expressing human EPAC1b with a C-terminal His10 tag and SUMO3(Q89K) was constructed using the pIRES2-EGFP vector (Clontech Catalog no. 632435). The EPAC1-His10-IRES-SUMO3(Q89K ...
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bioRxiv - Plant Biology 2020Quote: The total RNA of the buds collected for the transcriptome research was extracted by the EASYspin Plus Plant RNA Kit RN37 (Aidlab Biotechnology) and cDNA synthesis by the M-MLV RTase cDNA Synthesis Kit (TaKaRa Company). cDNA amplified fragment length polymorphism (cDNA-AFLP ...
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bioRxiv - Neuroscience 2021Quote: Total RNA from DRG was extracted using RNeasy micro kit and reverse-transcribed using PrimeScript RT Reagent Kit (Cat# RR037A, TaKaRa, Japan). Real-time PCR was performed using the SYBR Premix Ex Taq™ (Cat# DRR041A ...