Labshake search
Citations for Takara Bio :
4301 - 4350 of 5321 citations for Human Lysine K Specific Demethylase 1A KDM1A ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: ... A total of six libraries were generated using the ThruPLEX DNA-seq 6S (12) kit (Takara Bio Europe, TOWN, France). The concentration of each sample pool was measured with Quant-iT PicoGreen dsDNA assay kit (Invitrogen Life Technologies ...
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bioRxiv - Genetics 2023Quote: ... and subjected to quantitative real-time polymerase chain reaction with a TB Green Premix Ex Taq II kit (Takara, RR820A). The PCR program consisted of 95°C for 1 minute ...
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bioRxiv - Immunology 2023Quote: ... RNA-seq libraries were generated from 5 ng of RNA with the SMART-Seq v4 Ultra Low Input RNA Kit from Takara Bio (#634889 ...
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bioRxiv - Immunology 2023Quote: ... and sequencing libraries prepared from 10ng RNA using the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The RNA was quantified and 1.5 μg of RNA was used to prepare cDNA using PrimeScript 1st strand cDNA Synthesis Kit (Takara Bio) and random hexamer primer ...
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bioRxiv - Cancer Biology 2023Quote: ... The H2afx coding sequence from Mus musculus was ordered from Eurofins and cloned into the pOZ-N-FH backbone adding the 1xHA tag at the N terminus using the in-fusion HD cloning kit (#12141, Takara). Full length wild type H2afx coding sequence was then mutagenized to obtain the desired S139A point mutation.
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bioRxiv - Microbiology 2023Quote: ... The sequencing library was prepared using the SMART-Seq v4 Ultra Low Input RNA Kit with nonstranded and polyA+ selection (Clontech, Takara Bio USA ...
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bioRxiv - Plant Biology 2023Quote: ... and cDNA was synthesized with 2 μg RNA using PrimeScript™ 1st strand cDNA synthesis kit (TaKaRa, Kusatsu, Shiga, Japan) in a final reaction volume of 20 μl following the manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... and cloning it into the EcoRI and EcoRV sites of pcDNA-EGFP-P2A in frame with the tag sequence by using an In-Fusion® HD Cloning Kit according to the manufacturer’s instructions (Takara). pcDNA-hAPOBEC1 was constructed by cloning the XhoI-HindIII fragments of pUC57-APOBEC1 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Reverse transcription (RT) reactions were performed with 1 μg total RNA using the PrimeScript RT kit with gDNA Eraser (Takara). The resulting cDNA was amplified in triplicate on the 480 Real-Time PCR system (Roche ...
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bioRxiv - Biochemistry 2023Quote: ... Unique clones were sub-cloned into the sAB expression vector pRH2.2 (kind gift of S. Sidhu) using the In-Fusion Cloning Kit (Takara Bio). The best candidates (based on highest ELISA signals ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA encoding HsPINK1 was inserted into the BamHI site of pcDNA5/FRT/TO CMVd3 vector (pcDNA5d3, see (17)) using the In-Fusion HD Cloning Kit (Takara). For stable expression ...
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bioRxiv - Microbiology 2023Quote: ... This (DNA-free) RNA was reverse transcribed into cDNA by using PrimeScript RT reagent Kit with gDNA Eraser (Takara, Japan). Quantitative PCR was performed using a LightCycler 480 (Roche ...
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bioRxiv - Microbiology 2023Quote: ... These three amplified fragments were then seamlessly integrated into the Sac I site of YEP352-GAPII (Oka et al. 2007) using the In-Fusion HD Cloning Kit (Takara). Similarly ...
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bioRxiv - Biochemistry 2023Quote: The full-length SKP1-FBXO22 fusion protein with a 3xGGGS-linker was cloned into a pNic-Bio2 vector that encodes for an N-terminal His-tag as well as a C-terminal Avi-tag (GenBank: JF912191) using an In-Fusion HD Cloning System kit (Takara), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... double-stranded DNA library libraries from the size fractionated samples were constructed using the ThruPLEX DNA-seq Kit (Takara Bio) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... A cDNA library for TCR was prepared from RNA using a SMARTer Mouse TCR a/b Profiling Kit (Takara Bio) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... above 20% were used for downstream library generation using the SMARTer Stranded Total RNA – Pico RNA-seq kit v2 (Takara). Libraries were sequenced using 100 nucleotide paired-end sequencing on a NovaSeq 6000 (Illumina).
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bioRxiv - Microbiology 2023Quote: ... The viral RNA copy number was standardized with a SARS-CoV-2 direct detection RT-qPCR kit (Takara, Cat# RC300A). Fluorescent signals were acquired using a QuantStudio 1 Real-Time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2023Quote: ... SEAP activity in supernatant (25 μL) was measured using the Great EscAPe SEAP Chemiluminescence Assay kit 2.0 (Clontech, Fremonet, CA). The GLuc activity in supernatant (20 μL ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA was synthesized with the PrimeScript 1st Strand kit with an additional primer mix containing random DTs (#RR047A and #6110A, Takara). cDNAs were amplified using specific Taqman probes ...
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bioRxiv - Immunology 2023Quote: ... reverse transcription and cDNA pre-amplification were performed using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech/Takara). cDNA was harvested and quantified with the Bioanalyzer DNA High-Sensitivity kit (Agilent Technologies) ...
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bioRxiv - Genetics 2023Quote: ChIP-seq libraries were prepared starting with ∼1000 picograms of ChIP or input DNA samples using the ThruPlex DNA-seq kit (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... into 96-well plates containing 12.5μl CDS sorting buffer as described in the SMART-Seq® HT Kit protocol (Takara Bio). Full-length cDNA amplification of single cells was performed per the kit protocol (Takara Bio) ...
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bioRxiv - Microbiology 2023Quote: ... The resulting amplicons were cloned into pKTS786 (a pcDNA 3.1/V5-His based vector) that had been linearized with BstX1 with an In-Fusion HD cloning kit (Takara 638909) (76) ...
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bioRxiv - Molecular Biology 2023Quote: ... generated cDNA was subjected to real-time PCR analysis using SYBR® Premix Ex Taq™ II kit (TAKARA BIO) with the sets of specific primers (Table 1) ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were collected and mixed with Laemmli buffer after measuring the protein concentration using a TaKaRa BCA Protein Assay Kit (TaKaRa). Samples were subjected to SDS-polyacrylamide gel electrophoresis and electrotransferred onto polyvinylidene difluoride membranes ...
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bioRxiv - Genetics 2023Quote: ... First-strand cDNA was synthesized using 1.5 µg RNA and Prime Script™ RT reagent Kit with gDNA eraser (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... the transfer plasmid (pBABEpuro-based or pLZRS-IRES-ΔNGFR/GFP-based) and pCMV-VSV-G were co-transfected into GP2-293 packaging cells using CalPhos mammalian transfection kit (Takara/Clontech) according to manufacturer’s protocol ...
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Self-organised pattern formation in the developing neural tube by a temporal relay of BMP signallingbioRxiv - Developmental Biology 2023Quote: ... was digested with NheI and KpnI and the Lmx1a CDS and IRES-GFP fragments were inserted using the In-Fusion HD Cloning kit (Takara). Molecular biology kits used are listed in Table T4.
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bioRxiv - Cell Biology 2023Quote: ... and were reverse transcribed to first-strand cDNAs with random primers using PrimeScript First Strand cDNA Synthesis Kit (Takara, Japan). Quantitative PCR (qPCR ...
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bioRxiv - Cancer Biology 2023Quote: ... The number of virus copies/ml was determined using the Lenti-X™ qRT-PCR Titration Kit (Takara Bioscience, 631235) according to the manual ...
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bioRxiv - Genetics 2023Quote: ... Reverse transcription was performed using the oligo (dT) primer and AMV reverse transcriptase contained in the TaKaRa RNA PCR kit (TaKaRa). The RT-PCR was performed using KOD One PCR Master Mix (TOYOBO ...
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bioRxiv - Molecular Biology 2023Quote: ... These bulk kidney tissues were process from an OCT block using the SMARTer Stranded Total RNA-Seq Kit v2 (Takara). Sequencing was performed in a 2×75 bp paired-end configuration using a NovaSeq platform (Illumina).
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bioRxiv - Microbiology 2023Quote: ... The viral RNA copy number was standardized using a SARS-CoV-2 direct detection RT-qPCR kit (Takara, Cat# RC300A). Fluorescent signals from resulting PCR products were acquired using a Thermal Cycler Dice Real Time System III (Takara).
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bioRxiv - Cell Biology 2023Quote: ... The DNA fragment coding GFP was inserted at the 5’ side of SYP32 by In-Fusion HD Cloning Kit (Takara), and the whole sequence was recombined into pGWB1 by LR Clonase II ...
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bioRxiv - Biochemistry 2023Quote: Full-length HSF1 and its sequences were obtained by the rapid amplification of cDNA ends using a SMART cDNA amplification kit (Clontech). Gene-specific primers (GSPs ...
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bioRxiv - Immunology 2023Quote: ... cells were resuspended in Trizol reagent and frozen at -20°C until RNA was extracted using the Macherey-Nagel Nucleospin RNA XS Kit (CAS: 740902.50, Takara Bio) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Each single-cell RNA-seq library was generated from a single FGO or a single MII oocyte using SMART-Seq HT Kit (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... esrp1 and esrp2 were each cloned into a pCS2+8 plasmid backbone using the In-Fusion HD Cloning Kit (Clontech). The resulting pCS2+8-esrp2 plasmid was mutagenized with synonymous mutations surrounding the translational start-site using the GeneArt site-directed mutagenesis (SDM ...
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bioRxiv - Synthetic Biology 2024Quote: ... Ribo-seq RNA libraries were prepared for sequencing using the SMARTer® smRNA-Seq Kit for Illumina (#635030) from Takara Bio USA ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Libraries were harvested by scraping colonies from transformation plates and directly purifying plasmids (with no outgrowth that could introduce amplification bias) using Nucleobond Xtra midiprep kits (Takara).
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bioRxiv - Genomics 2024Quote: ... Alu6B and Aluh1.1 were synthesized as gene blocks (IDT) and cloned into BamHI-AatII sites in the pYa5-neo vector using Infusion cloning kit (Takara) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... ∼0.37 pg) per tube—was subjected to amplification using a PicoPLEX® Gold Single Cell DNA-Seq Kit (Takara Bio) and indexing primers (DNA HT Dual Index kit ...
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bioRxiv - Plant Biology 2024Quote: ... respectively) were inserted into the KpnI and XbaI sites of the vector using the In-Fusion HD Cloning Kit (TaKaRa).
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bioRxiv - Immunology 2024Quote: ... 100ng of total RNA was used for cDNA synthesis by RNA to cDNA EcoDry™ Premix (Double Primed) kit (Takara), and qPCR was performed by Taqman primers with TaqMan™ Fast Advanced Master Mix (Applied Biosystems™ ...
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bioRxiv - Bioengineering 2024Quote: ... The rituximab and cetuximab antibody variable sequences were generated by IDT and cloned into the variable regions of the VRC01 antibody plasmid vector (a generous gift from the Kim lab at Stanford) by using the In-Fusion cloning kit (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA synthesis and library preparations were performed by the Genomics Core using the SMARTv4 for ultra-low RNA input and Nextera XT kits (Takara). Sequencing was also performed on an Illumina NextSeq 2000 sequencing system with paired-end ...
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bioRxiv - Molecular Biology 2024Quote: ... AAV titer was determined by qPCR method using AAVpro Titration Kit (for Real Time PCR) Ver.2 (Takara-bio, #6233) following the manufacturer’s protocol.
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bioRxiv - Cell Biology 2024Quote: ... was inserted into pcDNA5-FRT/TO to generate pcDNA/TO/Dyn1-K44A-GFP using the In-Fusion cloning kit (Takara), and the following primers ...