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Citations for Takara Bio :
4201 - 4250 of 6263 citations for Triiodothyronine T3 ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The recombinant adenovirus was prepared in HEK293 cells and purified with an Adeno-X Virus Purification kit (Takara Bio). The purified virus titer was determined using an Adeno-X Rapid Titer kit (Takara Bio) ...
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bioRxiv - Neuroscience 2022Quote: ... and a subset (approximately 75,000 cells) was pelleted prior to RNA extraction using the NucleoSpin RNA Plus XS kit (Takara) following manufacturer protocol ...
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bioRxiv - Microbiology 2022Quote: ... cDNAs synthesized from the extracted total RNAs using a PrimeScript™ RT reagent Kit with gDNA Eraser (TAKARA, Japan) were used as templates to perform qPCR assays for the checking of the transcriptions of VEGFA ...
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bioRxiv - Biochemistry 2024Quote: ... This was followed by ligation of the guide RNA to the ligation oligo using the DNA Ligation Kit (Takara). Subsequently ...
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bioRxiv - Bioengineering 2024Quote: ... and reverse-transcribed into cDNA using PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (Takara Bio). The resulting cDNA was utilized for analysis using TB Green® Premix Ex Taq™ II (Tli RNaseH Plus ...
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bioRxiv - Cancer Biology 2024Quote: ... The cDNA synthesis and gDNA erase protocol were performed using the PrimeScript RT reagent Kit with gDNA Eraser (Takara). Quantitative real-time RT-PCR (qRT-PCR ...
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bioRxiv - Cancer Biology 2024Quote: ... and the remaining RNA isolation was done using the NucleoSpin RNA II Kit (Clontech Laboratories, Palo Alto, CA; 740955.50). 1 μg RNA was transferred into the cDNA Ecodry Premix Kit prior to the quantitative PCR program being run on ABI QuantStudio 3 with iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Genetics 2024Quote: ... cells were harvested and vectors were purified using the AAVpro purification kit (cat.: 6666; Takara Bio, Kusatsu, Shiga, Japan) as per manufacturer’s instructions and stored at -80 °C until further use ...
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bioRxiv - Bioengineering 2024Quote: ... [50] The ssDNA scaffold was initially prepared using Guide-it Long ssDNA Production System v2 kit (Takara Bio, 632666) following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Barcode sequence of each clone was PCR amplified directly from the cells using Terra PCR Direct Polymerase kit (Takara) for Sanger sequencing using previously described primers (5).
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bioRxiv - Cell Biology 2024Quote: ... Reverse transcription of RNA into cDNA was accomplished with the PrimeScript RT reagent Kit with gDNA Eraser (RR047A, Takara), followed by quantitative RT-PCR (qRT–qPCR ...
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bioRxiv - Cancer Biology 2024Quote: ... Reverse-transcription-quantitative PCR (RT-qPCR) was conducted using the Mir-X miRNA qRT-PCR TB Green Kit (TAKARA), the reverse-transcription samples ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... then cDNA was synthesized according to the PrimeScript TM II 1st Strand cDNA Synthesis Kit (Takara Bio, Dalian, China). Real-time PCR analysis was performed with the LightCycler 480 SYBR Green I Master (Roche ...
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MALAT1 mediates miR-206/CDC42/PAK1/Paxillin signaling axis to alleviate erectile dysfunction in ratsbioRxiv - Molecular Biology 2024Quote: ... and the extracted total RNA was used to remove DNA using the DNA Eraser Buffer kit (TaKaRa, Beijing, China). And the obtained RNA was used for reverse transcription using the PrimeScript RT Enzyme Mix I kit (TaKaRa) ...
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bioRxiv - Neuroscience 2024Quote: ... AAV was purified 3–5 days after transfection using AAVpro Purification Kit Midi or Maxi (Takara Bio, Shiga, Japan). The viral concentration was measured by qRT-PCR.
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bioRxiv - Molecular Biology 2024Quote: ... the cDNA was synthesized by reverse transcription using the PrimeScript RT Reagent Kit with gDNA Eraser (Takara, Dalian, China) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... a genomic DNA walk was carried out using the Universal Genome Walker 2.0 Kit (Takara Bio Inc. Company, USA). Genomic DNA was isolated using DNeasy ® Plant Mini Kit (Qiagen) ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA synthesis and amplification were performed using a SMART-Seq v4 Ultra Low Input RNA Kit for sequencing (Clontech). cDNA was purified using an Agencourt AMPure purification kit (Beckman Coulter) ...
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bioRxiv - Immunology 2024Quote: ... The isolated RNA was RNase III fragmented and adaptor-ligated using PrepX mRNA Library kit (Takara, Mountain View CA), then converted into cDNA using Superscript III reverse transcriptase (Lifetech ...
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bioRxiv - Genetics 2024Quote: ... Approximately 0.3 mg of total RNA was converted into cDNA with the PrimeScript RT Reagent Kit (Takara, Japan, RR037A) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... Library was prepared following the protocols provided by the manufacturer in SMART-Seq mRNA LP kit (Takara Bio #634768) and Unique Dual Index Kit (U001-U024 ...
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bioRxiv - Cell Biology 2023Quote: ... double-stranded cDNA was synthesized and amplified using the SMARTer Universal Low Input RNA Kit protocol (Clontech, No. 634938), a product optimized for archived biosamples.29 The cDNA was sheared by Covaris ...
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bioRxiv - Genomics 2023Quote: Five µg of each Quartet RNA sample was reverse transcribed using the PrimeScript™ RT reagent Kit (RR037A, TaKaRa) in a 50 µl reaction ...
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bioRxiv - Immunology 2024Quote: ... All ChIP-seq and input DNA libraries were made using ThruPLEX DNA-Seq kit (cat #R400674, Takara Bio, USA) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA was isolated from cells or tumors using a Mini BEST Universal RNA Extraction Kit (Takara, Shiga, Japan). cDNA was prepared from total RNA by reverse transcription using oligo-dT primers (Takara) ...
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bioRxiv - Biophysics 2022Quote: ... Unique clones were sub-cloned in pRH2.2 (a gift of S. Sidhu) using the In-Fusion Cloning kit (Takara) and sequence-verified ...
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bioRxiv - Molecular Biology 2023Quote: ... or into pHTN-HaloTag vector for GID4-HaloTag N-terminal fusion using the In-Fusion HD Cloning kit (Takara). Pro/N-degron coding sequence was cloned into pNLF1-C for MPGLWKS-NanoLuc C-terminal fusion ...
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bioRxiv - Molecular Biology 2023Quote: ... ChIP-seq libraries were prepared with sheared DNA (0.5 ng of ChIP DNA or 5.0 ng of input DNA) using ThruPLEX® DNA-seq Kit (Clontech). These obtained libraries were size-selected at 300-700 bp using ×1.0 AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Microbiology 2022Quote: ... using the primers listed in S-Table 1B into pcDNA3.1-P2A-tagRFP by the In-Fusion HD Cloning Kit (Takara), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... RNA amplification was performed using the SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (TaKaRa) and cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina) ...
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bioRxiv - Cell Biology 2022Quote: ... Reverse transcription was performed with 10 ng RNA using the SMART-Seq v4 Ultra Low Input RNA Kit (Takara) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Samples were then processed using the SMART-seq Stranded kit (Cat. 634444, protocol version 051018; Takara Bio USA, Inc.) according to the manufacturer’s protocol with minor modifications ...
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bioRxiv - Plant Biology 2023Quote: ... The gRNA was then ligated into BsaI linearized pKSE401 using DNA ligation Kit v.2.0 (TaKaRa, Kusatsu, Shiga, Japan).
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TIPRL1 and its ATM-dependent phosphorylation promote radiotherapy resistance in head and neck cancerbioRxiv - Cancer Biology 2023Quote: ... Wild-type (WT) TIPRL1 cDNA was cloned into 3xFLAG pLenti6.4 using In-Fusion® HD Cloning Kit (Takara, #639650). Phosphomimic and non-phospho mutants (S265D and S265A ...
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bioRxiv - Genomics 2023Quote: ... The digested and purified reporter pool was then ligated into 80 ng of the linearised entry vector using Takara ligation kit v1.0 (#6021; Takara) at a 1:3 (vector:insert ...
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bioRxiv - Microbiology 2023Quote: ... ssRNA was converted into cDNA using SMARTer Universal Low Input RNA Kit according to the manufacturer’s protocol (Takara Bio). After PCR amplification ...
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bioRxiv - Microbiology 2023Quote: ... The p24 amount in the medium was quantified by Lenti-X p24 Rapid Titer Kit (632200, Takara Bio Inc.) with the p24 control samples ...
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bioRxiv - Developmental Biology 2023Quote: ... RT-qPCR detection was performed on each group of samples by using a reverse transcription kit (Takara Bio, Japan) and fluorescence quantitative kit (Takara Bio ...
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bioRxiv - Immunology 2023Quote: cDNA was amplified from the collected cells using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech-TakaraBio). 1ng of amplified cDNA was used to generate barcoded libraries with the Nextera XT DNA library preparation kit (Illumina) ...
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bioRxiv - Cell Biology 2023Quote: ... Full length cDNAs were generated from 350 to 1,000 pg of total RNA using Clontech SMART-Seq v4 Ultra Low Input RNA kit for Sequencing (Takara Bio Europe ...
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bioRxiv - Neuroscience 2023Quote: ... mRNA was transcribed into full-length complementary DNA using a SMART-Seq v4 Ultra Low Input RNA kit (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were then lifted using TrypLE and total RNA was extracted using the Takara RNA plus kit following manufacturer’s instructions (Takara). 5 µL of total mRNA was converted to total cDNA using random hexamers following manufacturer’s instructions (Verso ...
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bioRxiv - Neuroscience 2023Quote: TUNEL assays were performed to detect apoptotic cell death using Clonetech ApoAlert DNA Fragmentation kit (Takara, Kusatsu, Shiga, Japan) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... Genomic DNA of the cell pellets was extracted and purified using a Nucleospin Blood XL kit (Takara Bio, #740950.10). Guides were amplified and barcoded by PCR using NEB Next Ultra ii Q5 MM (M0544L ...
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bioRxiv - Immunology 2023Quote: ... Samples were rRNA depleted and prepared for sequencing using SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Takara). In brief ...
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bioRxiv - Bioengineering 2023Quote: ... following which these sequences were inserted into the parental plasmid using In-Fusion® cloning kit (Takara Bio Inc.).
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bioRxiv - Cancer Biology 2023Quote: ... and used to produce barcoded cDNA sequencing libraries using the SMARTer® Universal Low Input RNA Kit (Takara, 634938). Libraries were sequenced on Illumina NovaSeq S4 (paired-end 2×150 ...
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bioRxiv - Cancer Biology 2023Quote: ... and used to produce barcoded cDNA sequencing libraries using the SMARTer® Universal Low Input RNA Kit (Takara, 634938). Validation of TT-TL procedure was performed by qPCR (TableS7 for primer sequences) ...
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bioRxiv - Bioengineering 2023Quote: ... The linearized pET28a vector and mfl fragment were ligated using the In-Fusion HD Cloning Kit (Clontech, Takara, Japan), and the ligated product was then transformed into E ...
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bioRxiv - Bioengineering 2023Quote: ... The linearized pET28a vector and mfl fragment were ligated using the In-Fusion HD Cloning Kit (Clontech, Takara, Japan), and the ligated product was then transformed into E ...