Labshake search
Citations for Takara Bio :
4101 - 4150 of 5356 citations for Pig Glucagon Like Peptide 2 GLP 2 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... which was further reverse transcribed into cDNA using a PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa, Tokyo, Japan), following the manufacturer’s instructions ...
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bioRxiv - Pathology 2020Quote: ... 3’-RACE-PCR was performed as per the instructions of SMARTer™ RACE-cDNA Amplification Kit User Manual (Clontech, 2012) using primers given in Table 1 ...
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bioRxiv - Immunology 2019Quote: ... Purified RNA was submitted to the Duke Sequencing and Genomic Technologies core facility for library preparation using the Clontech SMARTer v4 Ultra-Low kit (Takara). RNA-seq libraries were sequenced on either an Illumina HiSeq4000 flow cell as 50 bp single-end reads (for Foxp3YFP-cre ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA was extracted from E8.5 trunk tissue (two wild-type trunks and two Aldh1a2-/- trunks) and DNA sequencing libraries were prepared using the SMARTer Stranded Total RNA-Seq Kit v2 Pico Input Mammalian (Takara). Sequencing was performed on Illumina NextSeq 500 ...
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bioRxiv - Cell Biology 2019Quote: ... We then generated by PCR a Linker+ GFP/mCherry which we inserted into the dyn2 vector with the In-Fusion HD kit (Clontech). Dyn2-GFP-ΔCter and Dyn2-GFP-ΔPRD were generated introducing a stop codon at positions 807 (after motif A ...
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bioRxiv - Cancer Biology 2019Quote: ... The cDNA was then ligated into pCDH-CMV-MCS-EF1-GFP using Takara ligation Kit (Clontech, Mountain View, California, USA) and was confirmed in the expression vector by DNA sequencing at The Centre for Applied Genomics (SickKids ...
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bioRxiv - Plant Biology 2020Quote: ... Total amount of 1 μg RNA was used as template for first-strand cDNA synthesis with the oligo(dT) primer and the PrimeScriptTMRT reagent Kit with gDNA Eraser (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... 20 cells were sorted directly in cDNA synthesis buffer from the SMARTer Ultra Low RNA Kit for Illumina sequencing (Clontech) and used directly for cDNA synthesis ...
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bioRxiv - Molecular Biology 2019Quote: ... which were then cloned in the appropriate order into a pUC19 plasmid using an In Fusion HD Cloning Kit (Takara). The substrates were amplified using Phusion Polymerase PCR (NEB) ...
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bioRxiv - Cancer Biology 2019Quote: ... was excised and inserted in frame into a pcDNA3 vector containing fireflluciferase cDNA followed by a T2A sequence using the Infusion HD-eco dry kit (Clontech), as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... and then was used for generating the first strand complementary DNA (cDNA) as described in the protocol of the Takara PrimeScript RT reagent Kit with gDNA Eraser (Takara). Briefly ...
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bioRxiv - Plant Biology 2020Quote: ... Around 1μg RNA was taken for cDNA preparation by employing PrimeScript 1st strand cDNA Synthesis Kit (Clonetech Takara Bio, USA) (Chowrasia et al. ...
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bioRxiv - Immunology 2019Quote: Total RNA was added directly to lysis buffer from the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara), and reverse transcription was performed followed by PCR amplification to generate full length amplified cDNA ...
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bioRxiv - Immunology 2019Quote: ... Following the generation of cDNA using 5’RACE (rapid amplification of cDNA-ends; SMARTer RACE 5’/3’ Kit; Takara/Clontech), PCR amplification was performed using a Q5 polymerase (NEB ...
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bioRxiv - Immunology 2019Quote: ... Following the generation of cDNA using 5’RACE (rapid amplification of cDNA-ends; SMARTer RACE 5’/3’ Kit; Takara/Clontech), PCR amplification was performed using a Q5 polymerase (NEB ...
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bioRxiv - Developmental Biology 2020Quote: ... 700pg of total RNA were used for cDNA amplification with the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech-Takara). 1 ng of amplified cDNA was used to generate barcoded libraries using the Nextera XT DNA library preparation kit (Illumina) ...
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bioRxiv - Immunology 2019Quote: ... We used 0.2 μg of total RNA for reverse-transcription reactions using Advantage RT-for-PCR kit (TakaRa Bio USA). cDNA was used for RT-PCR ...
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bioRxiv - Bioengineering 2020Quote: ... Ligation products with the correct size were purified by DNA agarose gel extraction using NucleoSpin Gel and PCR Clean-Up Kit (Takara, 740609.250 ...
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bioRxiv - Plant Biology 2019Quote: ... EPFL2 promoter DNA was amplified by PCR from Col genomic DNA and inserted at the HindIII and SmaI sites of pPZP211/35S using the InFusion kit (Clontech) yielding the intermediate vector pPZP211/pEPFL2 ...
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bioRxiv - Microbiology 2019Quote: ... 5’-RACE cDNA was obtained from bulk-sorted splenic B cells of each mouse with SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (TaKaRa). The immunoglobulin PCRs were set up with Platinum Taq High-Fidelity DNA Polymerase (Life Technologies ...
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bioRxiv - Pathology 2020Quote: ... and then 8μL of each was reverse transcribed into cDNA by using the commercial company's reverse transcription kit (TAKARA No. 6110A). The full-length spike gene (3882 nt ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: 5’ and 3’ cDNA ends were subsequently amplified by RACE PCR using the SMARTer RACE cDNA Amplification Kit and Advantage HF2 DNA polymerase (Clontech) with gene-specific primers derived from the initial RT-PCR product (Supplemental Information ...
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bioRxiv - Genetics 2021Quote: ... This mutated fragment was replaced by the original homologous sequence of the pJet-ebony_mut-mCherry vector by fusing the mutated fragment and the PCR product amplified from the pJet-ebony-mCherry vector using the In-Fusion HD Cloning Kit (TaKaRa) with the following primers ...
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bioRxiv - Genetics 2021Quote: ... Reverse transcription was then performed using the oligo (dT) primer and Avian myeloblastosis virus reverse transcriptase contained in the TaKaRa RNA PCR kit (TaKaRa). We conducted RT-PCR using KOD One (TOYOBO ...
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bioRxiv - Genetics 2021Quote: ... fust-1 genomic fragment containing sequences from 2181bp upstream ATG to just before stop codon was cloned into the Sma I site of pHK-mRFP vector in frame with mRFP by In-Fusion HD Cloning Kit (Takara). This plasmid was further used to generate backbone structure containing fust-1 promoter and mRFP ...
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bioRxiv - Cell Biology 2019Quote: ... First-strand cDNA was synthesized from l μg of RNA using the PrimeScript RT Reagent Kit with gDNA Eraser (catalogue No. RR047A, TaKaRa). qPCR was performed in triplicate in 20-μl reactions containing SYBR Premix Ex Taq II (catalogue No ...
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bioRxiv - Microbiology 2021Quote: ... extracted RNA was reverse-transcribed into cDNA using the PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa, Dalian, China). The gene quantitative PCR was performed using TB green Premix EX Taq (Takara ...
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bioRxiv - Cell Biology 2020Quote: ... we first inserted the mAID sequence after 3xFlag in pCRISPaint-3xFlag-puro (Schmid-Burgk et al., 2016) using the In-Fusion HD Cloning kit (Takara) to generate pCRISPaint-3xFlag-mAID-puro ...
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bioRxiv - Immunology 2019Quote: ... cDNA libraries for alveolar macrophages were constructed and amplified using the SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian (Clontech) per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... was added to mitigate DNA contamination prior to first-strand cDNA synthesis with PrimeScript™ RT reagent kit (Takara Bio) from approximately 1 g total RNA ...
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bioRxiv - Neuroscience 2020Quote: ... Ten nanograms of total RNA were amplified and converted into cDNA using a SMART-Seq v4 Ultra Low Input RNA kit (Clontech). Next ...
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bioRxiv - Molecular Biology 2020Quote: ... pnYK vector was created from pnYC vector (58) by homologous recombination between the chloramphenicol and kanamycin genes resistance gene using the In-Fusion kit (Clontech) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2021Quote: ... Two-point five microliters of the polyadenylated sample was used for cDNA synthesis by the PrimeScript RT reagent kit with gDNA eraser (TaKaRa), with the y625 primer in Table S1 ...
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bioRxiv - Molecular Biology 2021Quote: ... Homology arms complementary to the pUC19 cloning vector were then added to the PCR product with another PCR in order to clone the RACE products with the In-Fusion HD Cloning kit (Takara). Individual clones were selected and Sanger sequenced.
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bioRxiv - Microbiology 2020Quote: ... Approximately 1,000 ng of RNA was reverse transcribed using the PrimeScript RT Reagent Kit with gDNA Eraser (TaKaRa, Dalian, China) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... For analysis of the NAD metabolome HEK293T cells were transfected with 1µg of pEGFP-C1 Empty Vector or pEGFP-C1-CMV-PARP10 using CalPhos Mammalian Transfection Kit (Takara Bio). 6 hours later the cells were treated with chemical treatments in DMEM + 10% FBS at 37°C 5% CO2 for 18h.
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bioRxiv - Microbiology 2020Quote: ... according to the manufacturer’s instructions and detected by RT-qPCR assays with a One-Step PrimeScript RT-PCR kit (Takara, Japan) using SARS-CoV-2-specific primers on an Applied Biosystems 7500 Real-time PCR System.
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bioRxiv - Synthetic Biology 2020Quote: ... The amplified fragments were gel-purified and fused using an In-Fusion® HD Cloning Kit (Takara Bio, Shiga, Japan). Full sequences of the plasmids used in this study are provided in Supplementary Information I ...
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bioRxiv - Bioengineering 2020Quote: ... and recovery of ribosome footprints were performed as described previously.[55] Footprints were dephosphorylated and cDNA libraries were prepared using the SMARTer smRNA-Seq Kit (Clontech), with an additional rRNA depletion step prior to PCR amplification ...
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bioRxiv - Pathology 2020Quote: ... Single stranded cDNA was synthesized with the oligo(dT) primer using PrimeScript™ RT reagent Kit with gDNA Eraser (Takara), the obtained cDNAs were analyzed by real-time PCR ...
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bioRxiv - Cell Biology 2020Quote: ... according to the manufacturer’s protocol.Forty ng cDNA was used as templates for RT-PCR using SYBR® Premix Ex Taq kit (TaKaRa,#RR820A) using LightCyclerW 480 (Roche ...
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bioRxiv - Cell Biology 2020Quote: Four hundred ng total RNA was employed for cDNA synthesis using ProtoScript II First Strand cDNA Synthesis kit(TaKaRa,#RR036A) according to the manufacturer’s protocol.Forty ng cDNA was used as templates for RT-PCR using SYBR® Premix Ex Taq kit (TaKaRa,#RR820A ...
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bioRxiv - Molecular Biology 2020Quote: ... The amplified DNAs were subcloned into the pCK-FLAG vector (CMV promoter-driven vector) at the BamHI and XhoI sites using In-Fusion HD Cloning Kit (Clontech). For dsRBD-deleted DICER ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The purification of the digested DNA and the ligation to the GenomeWalker adaptors were carried out according to the manufacturer’s instructions of the GenomeWalker Universal Kit (Clontech Laboratories). PCR reactions contained in a total volume of 50 µl 1x Long PCR buffer with MgCl2 ...
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bioRxiv - Plant Biology 2019Quote: ... and oligodT primer were used for cDNA synthesis with PrimeScript RT Reagent Kit (Perfect Real Time) following the manufacturer’s instructions (Takara Bio). An amount of 5 μl of 25X dilute cDNA was used as a template for ddPCR reaction (total 20 μl ...
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bioRxiv - Developmental Biology 2019Quote: ... 10 ng total RNA was reverse transcribed and full-length cDNA was specifically amplified by 8 PCR cycles using SMART-Seq v4 Ultra Low Input RNA Kit (Clontech) (31) ...
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bioRxiv - Plant Biology 2019Quote: Rapid amplification of 5’ cDNA ends (5’RACE) of CREF3 transcripts was performed using SMARTer RACE 5’/3’ Kit (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... Total RNA was used to synthesize cDNA with a PrimeScript II 1st Strand cDNA Synthesis Kit (D6210A; TaKaRa, Dalian, China) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... The cDNA was generated through a 3’-RACE-Ready cDNA synthesis reaction using the SMARTer RACE cDNA amplification kit (Clontech), following the user manual ...
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bioRxiv - Developmental Biology 2020Quote: ... and then further into the ClaI site of the avian replication-competent retrovirus vector RCASBP(B) (Li, Monckton, & Godbout, 2014) with the In-Fusion HD cloning kit (Clontech). The mutant construct that converts glutamine (Q ...