Labshake search
Citations for Takara Bio :
351 - 400 of 1168 citations for Mouse ADAMTS16 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2021Quote: ... Plasmid construction was performed using Infusion cloning enzymes (Takara Bio, Mountain View, CA).
-
bioRxiv - Neuroscience 2022Quote: ... for QF2 lines and NucleoBond Xtra Midi Endotoxin-Free plasmid kit (Clontech 740420.50) for Split-QF2 lines and eluted in ultrapure water prior to injection ...
-
bioRxiv - Neuroscience 2021Quote: ... The backbone plasmid used to transfect cell lines was pIRES2-EGFP2 (Clontech, UK). Coverslips were fixed with 4% paraformaldehyde (PFA ...
-
bioRxiv - Molecular Biology 2020Quote: ... Sequence was then inserted in the plasmid pEGFP-C1 (Clontech, Mountain View, CA) at Sma1 site ...
-
bioRxiv - Cell Biology 2022Quote: ... Digestion and ligation to BamHI and SalI-linearized pColdI plasmid (Takara, Shiga, Japan) were performed ...
-
bioRxiv - Molecular Biology 2021Quote: ... Mutations were introduced by whole-plasmid PCR using PrimeSTAR MAX DNA polymerase (Takara) according to the manufacturer’s protocol ...
-
bioRxiv - Genetics 2019Quote: ... Pairs of plasmids were transformed into strain Y187 (Clontech, Mountain View, CA, USA) and liquid β-galactosidase assays were performed using an OMEGASTAR plate reader (BMG Labtech ...
-
bioRxiv - Biophysics 2019Quote: ... and the mCherry gene was obtained from the pmCherry-N1 plasmid (Takara Bio). Reaction products were transformed ...
-
bioRxiv - Biochemistry 2020Quote: ... ADAR1 and PCBP2 cDNA were cloned from human thymus plasmid cDNA library (Clontech) using standard PCR techniques and then subcloned into indicated vectors ...
-
bioRxiv - Microbiology 2021Quote: ... fragments were PCR amplified from the plasmids using PrimeSTAR GXL Polymerase (Takara, Japan) and Nhel_IDT_F and EcoRI_ITD_R primers (Supplementary table 7) ...
-
bioRxiv - Cell Biology 2022Quote: ... GFP-tagged PDE5A isoforms plasmids [19] were co-transfected with pDsRed-mito (Clontech) into HEK293 cells using Lipofectamine 3000 (Life Technologies ...
-
bioRxiv - Plant Biology 2021Quote: ... The recombinant plasmid was transformed into Stellar™ Competent Cells (Clontech, Catalog # 636763) according to the manufacturer’s protocol and positive colonies were selected on LB plates containing kanamycin (50 μg/mL).
-
bioRxiv - Cell Biology 2021Quote: ... Plasmid DNA was transfected into A549 cells by Xfect transfection reagent (Takara Bio), while transfection of ON-Targetplus siRNAs (Dharmacon ...
-
bioRxiv - Genetics 2019Quote: ... HEK293T cells were transfected with wildtype or mutant SCN5A pIRES2-EGFP plasmids (Clontech) using FuGENE 6 (Promega ...
-
bioRxiv - Neuroscience 2019Quote: ... Concentrated lentiviruses for these two plasmids were made using Lenti-X concentrator (Takara) as has been done previously (Sun et al. ...
-
bioRxiv - Immunology 2020Quote: ... AP-1-Luc or NF-IL6-Luc plasmid (Clontech, Pal Alto, CA, USA) using Lipofectamine 2000 for 24 h (Stratagene ...
-
bioRxiv - Biochemistry 2019Quote: ... Any additional plasmids were constructed using backbone or insert PCR and inFusion (Clontech). Stellar competent cells were transformed by all the plasmids for amplification and DNA storage.
-
bioRxiv - Cell Biology 2021Quote: ... or cloned (all others) by standard mutagenesis techniques in the plasmid pQCXIP (Clontech). WT human PRRT2 (gene ID ...
-
bioRxiv - Neuroscience 2020Quote: Plasmids for lentivirus packaging were amplified by transformation into stellar competent cells (ClonTech) and extracted with endotoxin-free maxi-prep DNA extraction kit (Qiagen) ...
-
bioRxiv - Physiology 2019Quote: ... The purified DNA fragments were ligated into the pET-32a (+) plasmid (TaKaRa, Japan) and transformed into competent Escherichia coli DH5α cells ...
-
bioRxiv - Developmental Biology 2021Quote: ... These plasmids were transfected into Foxd3 cKO cells using Xfect transfection reagent (Clontech). The cells were kept under puromycin selection to obtain polyclonal cell lines ...
-
bioRxiv - Neuroscience 2021Quote: ... The sequence-verified plasmid was transfected into Lenti-X 293T cells (Takara Bio) together with the packaging plasmid psPAX2 (Addgene ...
-
bioRxiv - Cell Biology 2021Quote: ... we generate the plasmid pUAS-FMR1-ADARcd-V5-T2A-EGFP by Infusion (TaKaRa) cloning of the three PCR fragments ...
-
bioRxiv - Genetics 2022Quote: Plasmid constructs were generated using the In-Fusion HD Cloning Kit (Takara-bio). To construct pkd-2prom: ...
-
bioRxiv - Neuroscience 2022Quote: ... were amplified and purified by Nucleospin plasmid TF grade (Takara bio, Shiga, Japan). All plasmid sequences were verified using Sanger sequencing (3100 Genetic Analyzer ...
-
bioRxiv - Neuroscience 2023Quote: ... Reactions involving non-AAV plasmids were transformed into Stellar competent cells (Takara Inc.) and those involving AAV plasmids were transformed into NEB Stable competent cells (New England Biolabs).
-
bioRxiv - Plant Biology 2022Quote: The pGBKT7 and pGADT7 plasmids were co-transformed into yeast strain AH109 (Clontech) using the LiCl-PEG (polyethylene glycol ...
-
bioRxiv - Molecular Biology 2023Quote: ... All pHLP plasmids were created using the In-Fusion cloning system (Takara Biosciences) and were confirmed by Wide-seq analysis ...
-
bioRxiv - Developmental Biology 2023Quote: ... Plasmids were assembled from linear fragments using In-Fusion HD (Takara Bio, 638910) and amplified in Stellar chemically competent E ...
-
bioRxiv - Cell Biology 2023Quote: ... All plasmid ligation step was done using In-fusion seamless cloning (Takara Bio). Appropriate mutagenesis was done using QuikChange Lightning Multi Site-Directed mutagenesis kit (Agilent) ...
-
bioRxiv - Neuroscience 2023Quote: ... for plasmid library purification with NucleoBond® Xtra Midi EF kit (740420.10, Takara).
-
bioRxiv - Neuroscience 2023Quote: ... triple transfection of HEK-293T cells was performed using pHelper plasmid (TaKaRa/Clontech), the trans-plasmid providing viral capsid PHP.eB (a generous gift from Viviana Gradinaru ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Plasmid constructions were performed using In-fusion® HD-cloning kit (Takara©) in highly competent Stellar E ...
-
bioRxiv - Neuroscience 2023Quote: ... triple transfection of HEK-293T cells was performed using pHelper plasmid (TaKaRa/Clontech), the trans-plasmid providing viral capsid PHP.eB (a generous gift from Viviana Gradinaru ...
-
bioRxiv - Cell Biology 2024Quote: ... O’Sullivan and introduced into target plasmids through in-fusion cloning (#638948, Takara Bio). All the target plasmids in this study are derived from a plasmid that contains a CAG promoter for constitutive expression ...
-
bioRxiv - Microbiology 2024Quote: ... The coding sequence of YFP was amplified from the plasmid pEYFP-N1 (Clontech) using an upstream primer (YFP_F ...
-
bioRxiv - Immunology 2021Quote: ... Following bulk BCR and TCR are prepared using SMARTer Mouse BCR IgG H/K/L Profiling Kit and SMARTer Mouse TCR a/b profiling kit separately (Takara). Based on the extracted mRNA amount of each sample ...
-
bioRxiv - Molecular Biology 2020Quote: AQ-seq (bias-minimized sRNA-seq) libraries were constructed using total RNAs from fifteen mouse tissues (Mouse Total RNA Master Panel; Takara) as described in our previous study (Kim et al. ...
-
bioRxiv - Genetics 2021Quote: 1:500 mouse anti-GFP (Takara, JL-8)
-
bioRxiv - Cell Biology 2021Quote: ... mouse anti-GFP monoclonal JL8 (1:3000, Clontech), and mouse anti-α-tubulin monoclonal DM1A (1:3000 ...
-
bioRxiv - Microbiology 2021Quote: ... mouse anti-GFP (JL-8 clone, Takara Bio) or mouse anti-alpha-tubulin (Sigma) ...
-
bioRxiv - Neuroscience 2022Quote: ... and mouse anti-mCherry antibodies (Clontech; 1:2000) in PBST-azide with 3% NDS ...
-
bioRxiv - Molecular Biology 2019Quote: ... or a mouse α-GFP antibody (632381, Takara) to a dilution of 1:2000 in Odyssey Blocking Buffer (PBS ...
-
bioRxiv - Molecular Biology 2020Quote: ... RNAs from Mouse Total RNA Master Panel (Takara) were reverse-transcribed using the RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific ...
-
bioRxiv - Neuroscience 2020Quote: ... we used the normalized mouse brain library (Clontech Takara Bio ...
-
bioRxiv - Biochemistry 2021Quote: ... mouse GFP (1:4,000, Living Colors, 632380, Clontech) and rabbit β-Actin (1:3,000 ...
-
bioRxiv - Neuroscience 2021Quote: ... and mouse anti-mCherry antibodies (Clontech; 1:2000) in PBST-azide with 3% normal donkey serum ...
-
bioRxiv - Developmental Biology 2021Quote: ... and mouse anti-GFP (1:200; PA; Clontech). Alexa Fluor 488- ...
-
bioRxiv - Cell Biology 2021Quote: ... Mouse Anti-GFP (1:1000; JL-8, Clontech). Blots were washed three times with PBST and probed with secondary antibodies diluted in PBS with 1% milk and 1% BSA for one hour at 4°C ...
-
bioRxiv - Cancer Biology 2023Quote: ... and mouse anti-SPARC/Osteonectin (ON1-1, Takara) for 1 h at RT ...