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Citations for Takara Bio :
301 - 350 of 1168 citations for Mouse ADAMTS16 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... or mouse α-GFP antibody (632381, Takara) were diluted 1:2000 in Odyssey Blocking Buffer (PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... mouse anti-STEM121 (Takara, Y40410, 1:250), goat anti-ChAT (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... mouse anti-mCherry (632543, Clontech; 1:500), mouse anti-GFP (ab1218 ...
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bioRxiv - Biochemistry 2024Quote: ... GFP (mouse, Clontech cat# 632380; 1:2,000); FLAG (mouse ...
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bioRxiv - Microbiology 2020Quote: ... The ligated plasmid was then transformed to Escherichia coli HST08 (Takara Bio) and selected on chloramphenicol plates ...
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bioRxiv - Microbiology 2021Quote: ... and then was cloned in the plasmid pMD19-T simple (TAKARA, Japan). The DNA fragment was digested with BamHI/XhoI (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... pMD2.G and the CAR-expression plasmid by calcium phosphate transfection (Clontech). At 16 hrs post-transfection cells were washed with PBS and incubated in complete DMEM media containing 6 mM sodium butyrate (Sigma Aldrich) ...
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bioRxiv - Synthetic Biology 2022Quote: ... A middle-copy (∼10-30 copies) episomal plasmid pGADT7 (Takara Bio Inc.) was used to increase the concentration of proteins to buffer for the effects of intrinsic molecular noise and selected using different auxotrophic selection markers (Leucine ...
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bioRxiv - Cell Biology 2022Quote: All plasmids used were generated using the In-Fusion cloning system (Takara) with primers containing 15 bp 5’ flanking regions complementary to the free ends of the cloning vector ...
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bioRxiv - Genomics 2020Quote: ... After both ends of the plasmids were blunted using Klenow fragments (Takara), they were ligated by DNA ligase (Takara).
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bioRxiv - Cell Biology 2019Quote: Sources of plasmids encoding the following proteins were: mCherry-C1 (Clontech #632524); mCherry-N1 (Clontech #632523) ...
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bioRxiv - Immunology 2021Quote: ... and NF-kB reporter plasmids using X-fect Transfection reagent (Takara, Japan) following manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... The transgene plasmid was generated using the InFusion HD Kit (Clontech, 638910) and the NucleoBond Xtra Midi EF Kit (Macherey-Nagel ...
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bioRxiv - Biochemistry 2020Quote: ... The resultant plasmids were transformed into Stellar chemically competent cells (Clontech Laboratories), selected with carbenicillin ...
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bioRxiv - Neuroscience 2021Quote: Cloned plasmids were packaged in Lenti-X 293T cells (Takara Biosciences #632180), transfected using Lipofectamine 2000 (Thermo #11668027) ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting plasmid was then used for In-Fusion reaction (Takara Clontech) for inserting PCR-amplified GFP fragment at the C-terminus of AtSMOS1 ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting plasmid was then used for In-Fusion reaction (Takara Clontech) to insert PCR-amplified GFP fragments at the C-terminus of the SCL28 coding sequence ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting plasmid was then used for In-Fusion reaction (Takara Clontech) for inserting PCR-amplified GFP fragment at the C-terminus of AtSMOS1 ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting plasmid was then used for In-Fusion reaction (Takara Clontech) to insert PCR-amplified GFP fragments at the C-terminus of the SCL28 coding sequence ...
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bioRxiv - Neuroscience 2019Quote: ... and subcloned into the Cry2.mCh mammalian expression plasmids (mCherry-N1 (Clontech)) via restriction digest and ligation ...
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bioRxiv - Microbiology 2021Quote: ... were co-transformed with the plasmids pET-28b_atsJ and pG-KJE8 (TAKARA). A 200 ml culture in TB medium (50 μg/ml kanamycin ...
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bioRxiv - Microbiology 2020Quote: ... Transformation with plasmids and cultivation were performed using standard techniques (Clontech manual). In addition to negative and positive controls included in the MatchMaker III system ...
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bioRxiv - Cell Biology 2020Quote: The following plasmids were used in this study: pCDNA (control vector) (Clontech), pCDNA3-EGFP (Addgene Plasmid #13031) ...
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bioRxiv - Microbiology 2022Quote: ... and the PBSU101 plasmid was digested by BamHI and XbaI (Japanese Takara). Then PBSU101-pykF ...
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bioRxiv - Cancer Biology 2022Quote: The retroviral plasmid pRetroX-TetOne puro (634307, Clontech, Mountain View, CA, USA) was amplified using NEB Stable competent Escherichia coli (high efficiency ...
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bioRxiv - Immunology 2022Quote: ... Ligated plasmids were then transformed into Stellar competent cells (Takara Clontech, 636763) and single clones were picked on the next day ...
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bioRxiv - Immunology 2022Quote: ... Ligated plasmids were then transformed into Stellar competent cells (Takara Clontech, 636763) and single clones were picked on the next day ...
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bioRxiv - Neuroscience 2019Quote: ... The cloned plasmids were transformed into Stellar competent cells (Takara Bio, Japan), and 200 clones per rabies virus sample were isolated and purified for sequencing ...
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bioRxiv - Neuroscience 2019Quote: ... Plasmids used in this study were: pEGFP-N1 (Clontech, Mountain View, California); Flag-CNTNAP2 (generated by subcloning human CNTNAP2 cDNA from a plasmid kindly provided by Dr ...
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bioRxiv - Cell Biology 2019Quote: pEGFP-N1 (EGFP) and mCherry-C1 (mCherry) plasmids were purchased from Clontech Laboratories ...
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bioRxiv - Biochemistry 2019Quote: Recombinant DmNobo was expressed with the pCold-III plasmid vector (TaKaRa Bio) in Escherichia coli strain BL21(DE3 ...
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bioRxiv - Cell Biology 2020Quote: ... The sequence was ligated into the pLVX Puro lentiviral plasmid (Clontech, CA) using the same restriction sites and sequenced to confirm correct orientation ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmids were constructed using In-Fusion HD EcoDry Cloning Kits (Takara 121416), NEB HiFi assembly (New England Biolabs E5520S) ...
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bioRxiv - Immunology 2020Quote: The pLVX-IRES-ZsGreen1 plasmid (Takara Bio USA, Inc., Mountain View, CA) was used to generate construct encoding BiTE cDNA ...
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bioRxiv - Microbiology 2021Quote: ... and then was cloned in the plasmid pMD19-T simple (TAKARA, Japan). The DNA fragment was digested with EcoRI/XhoI and BamHI/EcoRI (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... The assembled plasmids were amplified by transformation of Stellar Competent Cells (Takara) and purified from overnight cultures in LB medium containing 100 μg/ml Ampicillin grown at 37°C by using the NucleoSpin Plasmid isolation kit (Macherey-Nagel) ...
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bioRxiv - Systems Biology 2022Quote: ... with pMI-NLS-Cre-ires-Tomato plasmid using the CalPhos kit (Clontech). Retroviral supernatants were harvested after 48hrs ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant plasmids were co-transformed into yeast Y2H Gold strain (Takara). The transformed yeast strains were grown on DDO (SD/-Leu/-Trp ...
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bioRxiv - Microbiology 2023Quote: ... and pMD2.G plasmids using TransIT-293 Transfection Reagent (TaKaRa, Cat# V2700) in Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: TurboID plasmids were made using In-Fusion Recombination (Takara Bio USA, Inc.). 3xHA-TurboID was amplified from 3xHA-TurboID-NLS pcDNA3 (Addgene plasmid #107171 ...
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bioRxiv - Cell Biology 2023Quote: ... and inserted into the pGEMHE plasmid backbone (In-fusion kit; Takara; 638948). Each CENP-O construct was tagged with GFP at the C-terminus ...
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bioRxiv - Cell Biology 2023Quote: ... plasmids were co-transfected into Lenti-XTM 293T cells (Clontech Laboratories, 632180) using Lipofectamine 3000 (Fisher ...
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bioRxiv - Developmental Biology 2023Quote: ... Addgene plasmid #17398) digested with EcoRI using In-Fusion HD system (Clontech). The fragments of interest were PCR amplified using high-fidelity Pfu DNA polymerase (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... the plasmid was constructed using the In-Fusion HD Cloning Kit (Takara); 1–2 kb sequences of the 5’ and 3’ ends of the genes of interest flanked the fragment that consisted of an in-frame linker ...
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bioRxiv - Plant Biology 2023Quote: ... the plasmid was constructed using the In-Fusion HD Cloning Kit (Takara); 1–2 kb sequences of the 5’ and 3’ ends of the genes of interest flanked the fragment that consisted of an in-frame linker ...
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bioRxiv - Microbiology 2023Quote: ... and pMD2.G plasmid with TransIT-293 Transfection Reagent (TaKaRa, Cat# V2700) in Opti-MEM (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids were transfected in HEK by using Xfect Transfection Reagent (631318, Takara) by following the user manual.
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bioRxiv - Microbiology 2024Quote: Plasmids were introduced into HEK293T cells using the TransIT®-293 (Takara) reagent in six-well plates ...
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bioRxiv - Plant Biology 2020Quote: ... The recombinant plasmids were transformed into Stellar™ Competent Cells (Clontech, Catalog # 636763), and positive colonies were selected on LB plates containing spectinomycin (100ug/mL ...
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bioRxiv - Biophysics 2021Quote: ... Five plasmids designed to express the following “chaperone teams” were purchased from TAKARA Bio Inc ...