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Citations for Takara Bio :
351 - 400 of 2435 citations for Human Chemokine C X C Motif Receptor 5 CXCR5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... using SARS-CoV-2 S as template and subcloned into pcDNA3.3 vector with a C-terminal hexahistidine tag using In-Fusion cloning technology (Takara Bio USA, Inc.). RBD of SARS-CoV S was cloned into pcDNA3.3 vector from pcDNA3.1-SARS-Spike (Addgene plasmid#145031) ...
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bioRxiv - Molecular Biology 2022Quote: ... was purchased from Eurofins Genomics and inserted in the C-termini of GFP with the In-Fusion HD cloning kit (Takara Bio USA). All primers used for the plasmid construction are listed in Table 3.
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bioRxiv - Molecular Biology 2023Quote: ... three individual transformants for each bait-prey combination were grown at 30°C in liquid Synthetic Dropout (SD; Takara Bio USA, www.takarabio.com) media lacking amino acids Trp (W ...
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bioRxiv - Cell Biology 2023Quote: ... clustering was performed by heterodimerization between LAMP1-mCherryFKBP (lysomes) and BicD-FRB (dynein adaptor) by the use of Rapalog (A/C Heterodimerizer ref. n°635057, from Takara Bio Inc.). Cells were cultured in glass bottom dishes and imaged at different time points (24 ...
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bioRxiv - Genomics 2024Quote: ... Membranes were then incubated overnight on a shaker at 4°C in primary antibodies (ß-actin CST3700S; MLH1 Invitrogen MA5-32041; Cas9 Takara 632607) diluted 1:1000 in 1x TBST with 3% BSA ...
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bioRxiv - Microbiology 2020Quote: For Bioluminescence Resonance Energy Transfer (BRET) assay we used the pEYFP-C1/N1 plasmids encompassing EYFP tag in the N- or C-terminal positions (Clontech, Mountain View, CA). To obtain pNluc-C1/N1 plasmids ...
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bioRxiv - Cell Biology 2019Quote: ... for 30 min at 37°C in a moisture chamber followed by an incubation with anti-GFP (1:100 in PBSA) antibody at 4°C for 72 h (Takara 632381/JL-8)) ...
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bioRxiv - Biophysics 2019Quote: ... The pCD86-EGFP had been created by inserting CD86 with a 21 amino acid linker coding region on its C-terminus immediately before EGFP in pEGFP-N1 (Clontech/Takara, Mountain View, CA). The CD86-21aa was amplified from pCD86-mEos2 (Mike Heilemann via Addgene ...
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bioRxiv - Immunology 2021Quote: ... or RARα403 (RARα-dAF2) 41 with a C-terminal Halo-tag were generated using the In- Fusion Cloning Kit (TaKaRa Bio Inc, Shiga, Japan). Lentiviruses were generated using CSII-EF- MCS-IRES2-Venus (for overexpression) ...
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bioRxiv - Neuroscience 2021Quote: ... All opsins were fused to mRuby2 at their C-terminus and sub-cloned into the pCAGGS expression vector by In-Fusion cloning (Clontech, Mountain View, CA). In order to target the opsins to the soma and proximal dendrites of neurons ...
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bioRxiv - Cancer Biology 2021Quote: All tissues from mice were quick-frozen in liquid nitrogen and stored at −80°C until they were dissolved with Trizol (TaKaRa, Guangzhou, China, A161050A). RNA extraction was performed according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2020Quote: ... for 1 h at 4 °C with rotation and then transferred to gravity columns (Talon® 2 ml Disposable Gravity Column, Clontech, #635606-CLI). The lysate was drained and the beads were washed five times with cold wash buffer (50 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... Synthesized gene fragments went through cloning process after overnight digestion with restriction enzymes (Nde I, Xho I) at 37 °C and ligation (T4 DNA Ligase, Takara Bio, Shiga, Japan) into pET21 vector (Novagen ...
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bioRxiv - Biochemistry 2021Quote: ... After sonication and centrifugation (20 000 g, 1 h, 4 °C) the supernatant was mixed with Talon Metal Affinity Resin (Takara Bio USA, Inc.). After 1 hour incubation at 4 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... Each sample was then incubated at 37°C for 30 min with 20 U of mRNA-Interferase™ MazF enzyme (Takara, Cat# 2415A) in a 10 μL volume reaction containing 1X MazF reaction buffer ...
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bioRxiv - Microbiology 2023Quote: ... or the BamHI/MluI site of pWPI-ACE2-zeo (for ACE2 expression plasmids)43 with 3×FLAG-tag at the C-terminus using In-Fusion® HD Cloning Kit (Takara, Cat# Z9650N). Nucleotide sequences were determined by DNA sequencing services (Eurofins) ...
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bioRxiv - Cell Biology 2023Quote: We used an hESC line that transiently expresses the C-terminal region (catalytic domain) of histone demethylase JMJD3 in a doxycycline (DOX, Takara Bio Inc, Japan) inducible manner (12 ...
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bioRxiv - Microbiology 2023Quote: ... and was then incubated with 2 mL TBS pH 8.0 pre-equilibrated Talon® Metal Affinity resin during 2 h at 4°C with permanent mixing (Takara Bio, Kusatsu, Japan). The resin was washed with 10 volumes of TBS pH 8.0 buffer containing 0.1% Triton X-100 and 10 mM imidazol (both from Sigma) ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5 μg of total RNAs were reverse-transcribed into cDNA at 42°C for 1 h in 20 μl reaction mixture containing mouse Moloney leukemia virus reverse transcriptase (PrimeScript, TAKARA BIO, Shiga, Japan) with random 6 primers (TAKARA BIO) ...
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bioRxiv - Neuroscience 2023Quote: ... Insoluble material was removed by centrifugation and the supernatant containing the receptor was rotated with Talon resin (Takara) overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... concentrated using Lenti X concentrator (Clontech), and added to H9 hESCs growing on Matrigel along with 4 µg/ml polybrene ...
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bioRxiv - Genetics 2021Quote: ... Lenti-X™ 293T Cells (Takara) were seeded on 10 cm dishes (6 × 106 cells per dish) ...
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bioRxiv - Molecular Biology 2020Quote: Lenti-X 293T cells (Clontech, 632180) were cultured for at least 24 hours in DMEM supplemented with 10% FBS and lacking antibiotics ...
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bioRxiv - Immunology 2021Quote: ... Lenti-X 293T cells from Takara Bio (632180 ...
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bioRxiv - Cancer Biology 2019Quote: HEK293 Lenti-X (Clontech, Cat. # 632180) cells were cultured in DMEM with 10% FBS ...
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bioRxiv - Developmental Biology 2020Quote: ... Lenti-X 293T cells (Clontech, 632180) were transfected with appropriate amounts of lenti-viral constructs ...
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bioRxiv - Biochemistry 2021Quote: Lenti-X 293T cells (Takara bio) grown in a 100 mm plate format were co-transfected with 4.5 μg psPAX2 (Didier Trono lab ...
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bioRxiv - Neuroscience 2021Quote: ... in Lenti-X 293T cells (Takara). Viral supernatant was collected at 48 and 72 hours post transfection and concentrated using Lenti-X Concentrator (Takara) ...
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bioRxiv - Microbiology 2020Quote: ... concentrated with Lenti-X (Takara Bio) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Lenti-X 293T cells (Takara #632180) were used for lentivirus packaging ...
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bioRxiv - Cell Biology 2020Quote: ... Lenti-X concentrator (PT4421-2, Clontech) was mixed at the ratio of 1:3 and incubated at 4 °C for a short time ...
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bioRxiv - Cell Biology 2022Quote: Lenti-X Expression System (Takara Bio) was utilized to generate lentivirus as per manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Lenti-X 293T cells (Takara Bio) were transfected with pHR SIN including cloned transgene and packaging vectors pMD2.G and pCMVdR8.91 using TransIT-Lenti Transfection Reagent (Mirus #MIR6603) ...
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bioRxiv - Genomics 2019Quote: Lenti-X 293T cells (Takara Biosciences) were used for lentivirus production ...
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bioRxiv - Developmental Biology 2022Quote: ... Clontech Lenti-X Concentration (Takara 631232) solution was used to concentration the LV batches by adding 5.0 ml of Lenti-X Concentrator to 15.0 ml of supernatant (1:3 ratio) ...
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bioRxiv - Cell Biology 2022Quote: ... incubated with Lenti-X concentrator (Clontech Laboratories ...
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bioRxiv - Cell Biology 2019Quote: ... into Lenti-X 293T cells (Clontech). Lenti-X 293T cells were seeded at 70% confluence to one 10 cm-dish 8 hours before transfection to reach about 90% confluence before transfection ...
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bioRxiv - Neuroscience 2020Quote: ... Lenti-X HEK 293T cells (ClonTech) were used for producing lentiviral particles ...
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bioRxiv - Immunology 2021Quote: ... Lenti-X 293T cells (Takara, 632180) were seeded in 10-cm2 dishes at a density of 5e6 cells per dish c and the following day transfected with 10 μg of spike expression plasmid with TransIT-Lenti (Mirus ...
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bioRxiv - Developmental Biology 2021Quote: ... Lenti-X 293T cells (Clontech, 632180) were cultured in DMEM (containing 1% penicillin/streptomycin ...
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bioRxiv - Microbiology 2021Quote: ... Lenti-X 293T cells (Takara, 632180) were seeded in 10-cm dishes at a density of 1e5 cells/cm2 and the following day transfected with 5 μg of spike expression plasmid with TransIT-Lenti (Mirus ...
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bioRxiv - Microbiology 2021Quote: ... Lenti-X 293T cells (Takara, 632180) were seeded in 10-cm2 dishes at a density of 5e6 cells per dish and the following day transfected with 10 μg of WT or B.1.617.2 spike expression plasmid with TransIT-Lenti (Mirus ...
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bioRxiv - Cancer Biology 2021Quote: ... concentrated using Lenti-X concentrator (Takara), and used immediately to transduce HCC827GR ...
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bioRxiv - Cancer Biology 2021Quote: ... concentrated using Retro-X concentrator (Takara), and used immediately to transduce human NSCLC cell lines ...
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bioRxiv - Microbiology 2020Quote: ... Lenti-X 293T cells (Takara Bio) were co-transfected with pLenti plasmids and the packaging plasmids psPAX2 and pMD2G/VSV-G (Addgene Plasmids #12259-60 ...
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bioRxiv - Immunology 2020Quote: Lenti-X™ 293T cells (Takara Bio ...
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bioRxiv - Immunology 2021Quote: ... Lenti-X-Concentrator (Takara Bio 631232) was added to the collected supernatant and lentivirus was concentrated following the manufacturer’s instructions and resuspended in OPTI-MEM in 1/100th volume of the original culture volume without supplements ...
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bioRxiv - Cell Biology 2021Quote: ... Lenti-X Concentrator (Takara, Cat. #631231) was used following the manufacturer’s protocol and resuspended in HBSS ...
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bioRxiv - Biophysics 2021Quote: ... and Lenti-X Concentrator (Clontech, 631232) was utilized to concentrate viral particles according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: ... concentrated overnight with Lenti-X (Takara), and frozen at −80°C ...