Labshake search
Citations for Takara Bio :
3851 - 3900 of 5356 citations for Pig Glucagon Like Peptide 2 GLP 2 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... The digested and purified reporter pool was then ligated into 80 ng of the linearised entry vector using Takara ligation kit v1.0 (#6021; Takara) at a 1:3 (vector:insert ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were then lifted using TrypLE and total RNA was extracted using the Takara RNA plus kit following manufacturer’s instructions (Takara). 5 µL of total mRNA was converted to total cDNA using random hexamers following manufacturer’s instructions (Verso ...
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bioRxiv - Bioengineering 2023Quote: ... following which these sequences were inserted into the parental plasmid using In-Fusion® cloning kit (Takara Bio Inc.).
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bioRxiv - Cancer Biology 2023Quote: ... and used to produce barcoded cDNA sequencing libraries using the SMARTer® Universal Low Input RNA Kit (Takara, 634938). Libraries were sequenced on Illumina NovaSeq S4 (paired-end 2×150 ...
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bioRxiv - Cancer Biology 2023Quote: ... and used to produce barcoded cDNA sequencing libraries using the SMARTer® Universal Low Input RNA Kit (Takara, 634938). Validation of TT-TL procedure was performed by qPCR (TableS7 for primer sequences) ...
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bioRxiv - Bioengineering 2023Quote: ... The linearized pET28a vector and mfl fragment were ligated using the In-Fusion HD Cloning Kit (Clontech, Takara, Japan), and the ligated product was then transformed into E ...
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bioRxiv - Bioengineering 2023Quote: ... The linearized pET28a vector and mfl fragment were ligated using the In-Fusion HD Cloning Kit (Clontech, Takara, Japan), and the ligated product was then transformed into E ...
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bioRxiv - Microbiology 2023Quote: ... and an aliquot of 100 ng was reverse transcribed with the PrimeScript RT Reagent Kit with gDNA Eraser (Takara) and the included primer mix ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNAs from the H1 and H2 hybridomas were purified using the NucleoSpin RNA purification kit (TAKARA, Tokyo, Japan) as described in the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Full-length cDNAs were generated directly from ~10,000 cells per sample using oligo(dT) priming and the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech), and 150 pg of cDNA was used to prepare strand-specific paired-end sequencing libraries (Nextera XT ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The product from this PCR was purified and cleaned with NucleoSpin Gel and PCR clean-up kit from TAKARA. A 2nd PCR was performed with high-fidelity PrimeSTAR GXL DNA Polymerase (Takara Cat ...
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bioRxiv - Biochemistry 2022Quote: ... total RNA (1.9 mg) was used to synthesize first-strand cDNA using the Mir-X miRNA First-Strand Synthesis Kit (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... was generated from a Katnal1 conditional KO targeting construct using the Guide-it Long ssDNA Strandase Kit (Takara, 632645). The following oligonucleotides were used to generate a PCR product from which the ssDNA was generated ...
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bioRxiv - Microbiology 2022Quote: ... Isolated RNA or serial ten-fold dilutions of RNA standards for the ORF1ab amplicon (ranging from 2.25×10^6 to 250 copies/rxn) were reverse transcribed using the Takara Prime Script RT kit (Takara) using poly(A ...
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bioRxiv - Developmental Biology 2023Quote: ... Start codon mutant constructs were generated by mutation PCR using the PrimeSTAR Mutagenesis Basal Kit (Takara Bio, Kusatsu, Japan) by replacing AUG with AAG at Met1 ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA amplification was performed using the SMART-Seq® v4 Ultra® low input RNA kit (Takara, cat. # 634891) with sixteen cycles of PCR ...
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bioRxiv - Developmental Biology 2023Quote: ... and the viral packing vectors (pCMV-ΔR8.9 and pCMV-VSV-G) using the CalPhos mammalian transfection kit (631312, Clontech). pCW57.1-Luciferase and pCW57.1-mDux-CA were a gift from Stephen Tapscott (Addgene plasmids #99283 and #99284) ...
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bioRxiv - Cancer Biology 2023Quote: ... Synthesis of cDNA and library preparation were performed using the SMARTer Universal Low Input RNA Kit for Sequencing (Clontech) and the Ion Plus Fragment Library Kit (ThermoFisher ...
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bioRxiv - Cancer Biology 2023Quote: ... 1ng of total RNA was pre-amplified with the SMARTer Ultra Low Input kit v4 (Clontech, Mountain View, CA) per manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR amplified and subcloned into a pCG-strep expression construct using the In-Fusion® HD Cloning Kit (Takara) according to the manufacture’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... total RNA was collected using a CellAmp Direct RNA Prep Kit for RT-PCR (Real Time) (TaKaRa, Cat# 3732) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: Full-length cDNA was synthesized with 1.0 μg RNA from Diphasiastrum complanatum using the SMARTer PCR cDNA Synthesis kit (Clontech). The first-strand cDNA was amplified with PrimeSTAR GL DNA polymerase (Clontech ...
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bioRxiv - Biochemistry 2023Quote: ... cDNA was prepared from 1 µg of total RNA using the PrimeScript RT Reagent Kit with gDNA Eraser (Takara). Real-time polymerase chain reaction (real-time PCR ...
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bioRxiv - Genomics 2023Quote: ... Genomic DNA of the cell pellets was extracted and purified using a Nucleospin Blood XL kit (Takara Bio, #740950.10). Guides were amplified and barcoded by PCR using NEB Next Ultra ii Q5 MM (M0544L ...
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bioRxiv - Immunology 2023Quote: ... Samples were rRNA depleted and prepared for sequencing using SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Takara). In brief ...
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TIPRL1 and its ATM-dependent phosphorylation promote radiotherapy resistance in head and neck cancerbioRxiv - Cancer Biology 2023Quote: ... Wild-type (WT) TIPRL1 cDNA was cloned into 3xFLAG pLenti6.4 using In-Fusion® HD Cloning Kit (Takara, #639650). Phosphomimic and non-phospho mutants (S265D and S265A ...
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bioRxiv - Neuroscience 2023Quote: ... and the target bands at 14,438 bp were cut and purified with NucleoSpin® Gel and PCR Clean-Up kit (740609.250, Takara). The target amplicons were re-concentrated to > 300 ng/ µL with GlycoBlue™ Coprecipitant (AM9515 ...
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bioRxiv - Genomics 2023Quote: Five µg of each Quartet RNA sample was reverse transcribed using the PrimeScript™ RT reagent Kit (RR037A, TaKaRa) in a 50 µl reaction ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were prepared by the University of Minnesota Genomics Center using the SMARTer Pico Mammalian V2 kit (Takara). For RNA-Seq from EVTs and STBs ...
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bioRxiv - Genetics 2023Quote: ... Library was prepared following the protocols provided by the manufacturer in SMART-Seq mRNA LP kit (Takara Bio #634768) and Unique Dual Index Kit (U001-U024 ...
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bioRxiv - Cell Biology 2023Quote: ... double-stranded cDNA was synthesized and amplified using the SMARTer Universal Low Input RNA Kit protocol (Clontech, No. 634938), a product optimized for archived biosamples.29 The cDNA was sheared by Covaris ...
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bioRxiv - Biophysics 2023Quote: ... All remaining unsorted cells in addition to the sorted cell populations were then miniprepped (Takara NucleoSpin Plasmid miniprep kit), and DNA was stored at 4 °C short-term or at −20 °C for long-term storage.
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bioRxiv - Biophysics 2023Quote: ... Viral vector titers were determined by the number of DNase I resistant vg using AAV titration kit (TaKaRa/Clontech) by qPCR (StepOne ...
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bioRxiv - Biophysics 2023Quote: ... Viral vector titers were determined by the number of DNase I resistant vg using AAV titration kit (TaKaRa/Clontech) by qPCR (StepOne ...
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bioRxiv - Immunology 2023Quote: ... S-IgA was purified by two-step chromatography using the Capturem His-Taged Purification Kit (Takara Bio, Shiga, Japan) followed by size exclusion chromatography (Cytiva ...
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bioRxiv - Cancer Biology 2023Quote: Real-time PCR was carried out using a TB Green Premix Ex Taq (Tli RNaseH Plus) kit (Takara, Japan) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: The plasmids of TiCGSCy mutants (E1442Q, E1442A and E1356A) were constructed using a PrimeSTAR mutagenesis basal kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The mutant 2X FLAG TANGO2 Gly154Arg was generated with the In-Fusion HD Cloning Plus Kit (Takara Bio Europe) following the manufacturer’s instructions using the pMK-T plasmid as template ...
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bioRxiv - Microbiology 2023Quote: ... total RNA was collected using a CellAmp Direct RNA Prep Kit for RT-PCR (Real Time) (TaKaRa, Cat# 3732). GAPDH and vRNA levels were measured with a RT-qPCR assay using the One Step TB Green PrimeScript PLUS RT-PCR Kit (Perfect Real Time) ...
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bioRxiv - Immunology 2024Quote: ... Total RNA Sequencing libraries were prepared with SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (TaKaRa) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2024Quote: The pool of duplets was ligated to each of the barcoded promoter vectors using Takara ligation kit version 2.1 (#6022; Takara). Ligation products were purified using magnetic bead purification and 2 µl of the ligation were electroporated into 20 µl of electrocompetent e ...
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bioRxiv - Plant Biology 2024Quote: ... RT-qPCR analysis was then performed with 1.0 μl of 5-fold diluted cDNA using a SYBR premixed Ex Taq kit (Takara), and a Light Cycler 480 Real-Time PCR System (Roche ...
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bioRxiv - Neuroscience 2024Quote: ... 1µg of total RNA was mixed with a total volume of 10µl of cDNA synthesis kit (TaKaRa 6110A, Tokyo) consisting of dNTP mixture ...
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bioRxiv - Molecular Biology 2024Quote: ... LTR amplicons or gBlocks™ Gene Fragments were mixed with the pLd2G backbone (gLTR_OUT) and genomic (gLTR_IN) amplicons and joined in a single reaction using In-Fusion® HD Cloning Kit (Takara).
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bioRxiv - Biophysics 2024Quote: ... The resulting product was separated on a 0.75 % agarose gel and the 11.6-kb dsRNA was excised and purified using Agarose Gel DNA Extraction Kit (Takara).
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bioRxiv - Bioengineering 2024Quote: ... PCR products were then isolated through gel electrophoresis and purified using the Nucleospin gel purification kit (Takara Bio,740609.250). Linear DNA fragments were then ligated using inFusion assembly and amplified in Stellar competent Escherichia coli (Takara Bio ...
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bioRxiv - Genetics 2024Quote: ... total RNA libraries were prepared by using SMART-Seq v4 Ultra® Low Input RNA Kit for Sequencing (Clontech) according to the manufacture’s instruction and analyzed by Illumina HiSeq 2500 system (Illumina) ...
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bioRxiv - Developmental Biology 2024Quote: ... Sequencing was performed using SMARTer chemistry (SMARTer Ultra Low RNA Kit for the Fluidigm C1 System, Clontech 634835/634935) (three retinae ...
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bioRxiv - Microbiology 2024Quote: Total RNA extracted from homogenized lung tissues was reverse transcribed into cDNA using MiniBEST Universal RNA Extraction Kit (Takara) and PrimeScriptTM RT reagent kit (Takara) ...
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bioRxiv - Microbiology 2024Quote: ... 90 μl of RNase-free water was added and then 2.5 μl of diluted sample was used for real-time RT-PCR according to the manufacturer’s protocol with One step TB green PrimeScript PLUS RT-PCR Kit (Takara) and primers for the nucleocapsid (N ...