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Citations for Takara Bio :
301 - 350 of 939 citations for Tetanus Toxoid Recombinant Heavy Chain Fragment C since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... Adaptor-ligated fragments were amplified using the Advantage® GC 2Polymerase mix (Clontech Takara) (primer described in Table MM ...
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bioRxiv - Genetics 2021Quote: ... Fragments after electrophoresis were recovered using NucleoSpin Gel and PCR Clean-up (Takara Bio). The nucleotide sequences were determined by the Yamaguchi University Center for Gene Research ...
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bioRxiv - Neuroscience 2019Quote: ... or IMPA1L 120nt fragment were labeled using Random Priming Labeling Kits (Takara or Roche) and [α−32P] dCTP ...
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bioRxiv - Molecular Biology 2019Quote: ... All constructions were obtained by assembling PCR fragments using InFusion® cloning kit (Takara). Using appropriate sets of oligonucleotides ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA fragments larger than 3 kb were amplified with PrimeSTAR Max (Clontech Laboratories, Inc.); all other PCR products were amplified with PrimeSTAR HS DNA polymerase (Clontech Laboratories ...
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bioRxiv - Plant Biology 2022Quote: ... and Nos terminator (NosT) fragments were combined with In-Fusion HD mix (Takara Bio) and cloned to Escherichia coli ...
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bioRxiv - Neuroscience 2023Quote: ... DNA fragments were ligated together using an In-Fusion Snap Assembly Master Mix (Takara). The sgRNA sequences for knock-in and knockout are listed in the supplemental materials table ...
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bioRxiv - Cell Biology 2023Quote: ... The DNA fragment of RFP-CaaX was inserted into the pEGFP-C1 vector (Clontech) to replace the EGFP for the mammal expression ...
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bioRxiv - Developmental Biology 2023Quote: ... The PCR amplified cDNA fragments were cloned into PEGF-N1 vector (Clontech,6085-1,) with XhoI/BamhI enzyme sites ...
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bioRxiv - Cell Biology 2023Quote: ... Fragments of interest were amplified using the EmeraldAmp GT PCR Master Mix (Takara, #RR310B) and intron- or exon - specific forward primers combined with a common exon junction-specific reverse primer ...
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bioRxiv - Microbiology 2024Quote: ... DNA adapters were ligated to MmeI-digested DNA fragments by T4 DNA ligase (Takara), and the ligation products were purified on magnetic beads ...
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bioRxiv - Developmental Biology 2024Quote: ... into which a DNA fragment was inserted by In-Fusion HD Cloning Kit (TAKARA) to produce RCAS-GCaMP6s-P2A-mRuby3 ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative real-time polymerase chain reaction (qRT-PCR) was carried out using the TB GreenTM Premix Ex TaqTM II (Takara Bio Inc., Kusatsu, Japan) according to the manufacturer’s protocol for a total reaction volume of 20 μl ...
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bioRxiv - Immunology 2020Quote: ... cDNA was generated using a template□switch anchored reverse□transcription polymerase chain reaction (RT□PCR) based on the SMARTer pico cDNA PCR Synthesis Kit (Clontech, Mountain View, CA, USA). The final cDNA product was then subjected to a clean□up step using PCR Clean DX Beads (Aline Biosciences ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.3 μl of lysis buffer (0.13% Triton-X-100, 4 units of recombinant RNase Inhibitor, Takara) was added to 3.7 μl of cell-free CSF and plasma ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The recombinant Irg1-like protein was enriched using cobalt TALON Metal Affinity Resin (Takara Bio #635501) with gentle shaking for 1 hour at 4 ℃ and washed twice by centrifugation for 3 minutes at 3500 rpm at 4 ℃ and resuspension in the washing buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Cancer Biology 2022Quote: ... Recombinant AAV2 was packaged in HEK293T cells using pHelper and pRC2-mi342 plasmids (Takara, Catalog #632608). Three days after transfection ...
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bioRxiv - Cell Biology 2021Quote: ... The recombinant protein was produced in Escherichia coli BL21 (DE3) and purified with TALON resin (Clontech). For immunization ...
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bioRxiv - Cell Biology 2023Quote: ... His-tagged recombinant proteins were isolated by 1 h incubation with Talon metal affinity resin (Clontech) at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... under standard conditions with oligo(dT) or random hexamer primers and Recombinant RNase Inhibitor (RRI, TAKARA). Then the cDNA was subjected to quantitative RT-PCR (qRT-PCR ...
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bioRxiv - Genomics 2023Quote: ... Recombinant AAV2 was packaged in HEK293T cells using pHelper and pRC2-mi342 plasmids (Takara, Catalog #632608). Three days after transfection ...
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bioRxiv - Neuroscience 2023Quote: Each lysis plate well contained 4uL of lysis buffer (4U Recombinant RNase Inhibitor (Takara Bio 2313B), 0.05% Triton X-100 ...
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bioRxiv - Cell Biology 2022Quote: ... 200 μM A/C heterodimerizer (Takara) or ethanol control ...
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bioRxiv - Neuroscience 2020Quote: ... Rapalog (A/C Heterodimerizer, TaKaRa, #635056) was added manually to a concentration of 100 nM to the cell medium ...
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bioRxiv - Neuroscience 2020Quote: ... rapalog (A/C Heterodimerizer, TaKaRa, #635056) was added at a final concentration of 100 nM.
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bioRxiv - Cancer Biology 2023Quote: ... were fused to GFP-C (Clontech). mCherry LEGO-C2 was provided by Dr ...
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bioRxiv - Molecular Biology 2020Quote: ... p30 or p30 R3L-BirA Ligase fragments were cloned into the pENTRY2B vector (Clontech #3064) using BamHI and XhoI restriction sites and subsequently introduced into the pInducer21 GFP lentiviral vector (Clontech #3044 ...
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bioRxiv - Molecular Biology 2019Quote: ... The fragment amplified from Isoform000014 was purified and cloned to pMD-19T vector (Takara, China) followed by Sanger sequencing.
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bioRxiv - Plant Biology 2020Quote: ... The amplified fragment was introduced into the PstI/XbaI sites of pRI201-AN (TAKARA Bio), and the construct was designated as pMYC241 ...
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bioRxiv - Microbiology 2020Quote: ... and each amplified fragment was cloned into pET-16b by In-Fusion Cloning (Takara Bio). The nucleotide sequences of the inserts were confirmed by sequencing ...
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bioRxiv - Molecular Biology 2020Quote: ... The tIE2.1 and tIE2.2 promoter fragments were then cloned into pEGFP-N1 vector (Clontech, USA) to produce pCMV/tIE2.1 and pCMV/tIE2.2 vectors ...
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bioRxiv - Cell Biology 2019Quote: ... Fragments and vector backbone were recombined using in-Fusion HD cloning plus CE (Clontech, 638916) to create a functional circular plasmid ...
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bioRxiv - Neuroscience 2022Quote: ... We inserted the PCR fragments at NheI/BamHI sites by infusion HD-cloning (Takara Biosciences), to excise HA-tag from the plasmid ...
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bioRxiv - Microbiology 2022Quote: ... Fragments were cloned into a PvuII linearized pUC19 using In-Fusion HD cloning kit (TaKaRa) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... RNA was transcribed from a DNA fragment using an in vitro Transcription T7 kit (TAKARA).
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bioRxiv - Plant Biology 2022Quote: ... we fused proAtSPCH and MpSETA CDS fragments by PCR using PrimeSTAR GXL polymerase (Takara Bio). The resultant PCR fragment was subcloned into pENTR1A entry clones (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... The three DNA fragments were assembled using In-fusion HD cloning kit (Takara, Kusatsu, Japan). The resultant DNA construct (pCM43b-mCherry:GFP:Stx17 ...
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bioRxiv - Microbiology 2019Quote: ... The obtained DNA fragments were ligated using an In-fusion HD cloning Kit (Takara Bio) to yield pET50b-Amp ...
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bioRxiv - Cell Biology 2021Quote: ... Fragments for the assembly reaction were generated with CloneAmp™ HiFi PCR Premix (Takara Clontech) using the following primers ...
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bioRxiv - Cell Biology 2021Quote: ... Fragments for the assembly reaction were generated with CloneAmp™ HiFi PCR Premix (Takara Clontech) using the following primers ...
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bioRxiv - Genetics 2022Quote: The 5’end of the cloned fragment was amplified with a 5’RACE kit (Takara). The 5’RACE adaptor in the kit was used to evaluate the mRNA ...
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bioRxiv - Genetics 2022Quote: The 3’end of the cloned fragment was amplified with a 3’RACE kit (Takara). RNA from fresh soybean roots was used as the template ...
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bioRxiv - Plant Biology 2022Quote: ... Amplified fragments were inserted into the pUC18 vector containing pro35S:sGFP:NOS terminator by in-fusion (TaKaRa). Before transfection ...
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bioRxiv - Plant Biology 2022Quote: ... The amplified fragments were cloned into the Xba I/Sac I site of pBI121 (Clontech) located downstream of the cauliflower mosaic virus (CaMV ...
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bioRxiv - Neuroscience 2022Quote: The jGCaMP7f fragment was generated using PCR with PrimeSTAR Max DNA Polymerase (TaKaRa, Shiga, Japan) on a PCR thermal cycler (TP350 ...
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bioRxiv - Neuroscience 2022Quote: ... DNA fragments of OR genes were amplified by PrimeStar MAX polymerase (Takara bio, Shiga, Japan). The fragments were mixed and amplified by PCR reaction to obtain full sequences ...
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bioRxiv - Molecular Biology 2022Quote: ... The DNA fragments for cloning were amplified using PrimeStar Max DNA Polymerase (R045A, Takara Bio) and the primer sets listed in Supplementary Table S2 ...
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bioRxiv - Microbiology 2023Quote: ... sequence fragments were amplified from the complete hnRNPM cDNA using PrimeSTAR Max DNA Polymerase (TaKaRa) with primer sets no ...
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bioRxiv - Bioengineering 2023Quote: ... DNA fragments were amplified with the high-fidelity polymerase PrimeSTAR Max DNA Polymerase from TaKaRa and colony PCR reactions were performed using DreamTaq polymerase (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: Linear DNA fragments were amplified via PCR using CloneAmp HiFi PCR premix (Takara Bio, 639298). PCR products were cut from agarose gels and purified using the Nucleospin gel purification kit (Takara Bio ...