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Citations for Takara Bio :
3551 - 3600 of 4901 citations for Uridine Triphosphate UTP CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... The amplicons from SlACS2-prom1k-pPLV-F/R were cloned into pPLV451 using an In-Fusion HD Cloning kit (Clontech), to construct pACS2-GFPx3 and pACS2mut-GFPx3 ...
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bioRxiv - Microbiology 2020Quote: ... gene was then PCR amplified and purified then cloned into plasmid pLC29144 using In-Fusion HD Cloning plus kit (Takara). All PCR products and linearized vector were purified (Macherey and Nagel ...
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bioRxiv - Microbiology 2020Quote: ... overhangs upstream and downstream of the gene of interest were amplified (primers sequences available in Table S1) and purified then cloned into linearized pCM433 using In-Fusion HD Cloning plus kit (Takara), primer design was done using primer design tool (Takara) ...
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bioRxiv - Developmental Biology 2021Quote: ... Libraries were prepared from 50 ng of total RNA/sample as input using Ribogone and a SMARTer Universal Low Input RNA Kit (Clontech). For inDrops ...
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bioRxiv - Molecular Biology 2020Quote: Primary RT-PCR and secondary PCR was performed using the Primescript III 1-step RT-PCR kit and EmeraldAmp GT PCR Mix (Takara; detailed protocol provided in Supplementary File 1) ...
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bioRxiv - Genomics 2019Quote: ... The IsoSeq libraries were created starting from 1µg of total RNA per sample and full-length cDNA was then generated using the SMARTer PCR cDNA synthesis kit (Clontech) following PacBio recommendations set out in the IsoSeq method (pacb.com/wp-content/uploads/Procedure-Checklist-Iso-Seq-Template-Preparation-for-Sequel-Systems.pdf) ...
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bioRxiv - Molecular Biology 2019Quote: ... cerevisiae CLN2 was appended to the C-terminal codon of the HIS3 gene using the In-Fusion Cloning Kit (Clontech) as previously described (2) ...
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bioRxiv - Genomics 2021Quote: ... These nanowells were selected for subsequent targeted deposition of 50□nl/nanowell RT-PCR reaction mix from the SMARTer ICELL8 3’ DE Reagent Kit (Takara) using the MSND ...
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bioRxiv - Neuroscience 2021Quote: ... Ten ng of total RNA were amplified and converted into cDNA using a SMART-Seq v4 Ultra Low Input RNA Kit (Clontech). Next ...
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bioRxiv - Neuroscience 2020Quote: The cDNA libraries for used for sequencing of 4 total RNA samples were synthesized using a SMART-Seq Ultra Low Input RNA kit (Takara) in the OHSU Massively Parallel Sequencing Shared Resource Core Facility ...
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bioRxiv - Microbiology 2021Quote: ... PCR amplifications were conducted in 50-μL total volumes containing 1 μL of template DNA using an ExTaq kit (Takara). The cycling protocol was as follows ...
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bioRxiv - Plant Biology 2021Quote: Tagmented DNA was amplified directly from the beads via 6 cycles of PCR amplification using the PrimeSTAR GXL Polymerase kit (Takara) and dual indexed adapters (Illumina ...
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bioRxiv - Neuroscience 2021Quote: RNA from 30,000 cultured adult NSCs or whole DGs were isolated with the Clontech Nucleospin RNA XS Plus isolation kit (Takara 740990.10) per manufacturer protocol ...
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bioRxiv - Plant Biology 2021Quote: ... which was used to synthesize first-strand cDNA using the SuperScript II first-strand cDNA synthesis Kit™ (Takara, Japan). The Arabidopsis Actin2 gene was used as the internal reference gene.
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bioRxiv - Microbiology 2020Quote: The DNA samples were prepared for Illumina sequencing using the SMARTer® ThruPLEX® DNA-Seq kit (Takara Bio, USA) according to manufacturer protocol ...
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bioRxiv - Immunology 2020Quote: ... Libraries were constructed from total RNA with the ‘SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian’ (Takara/Clontech) according to manufacturer’s recommendations ...
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bioRxiv - Immunology 2020Quote: ... Libraries were constructed from total RNA with the ‘SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian’ (Takara/Clontech) according to manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2020Quote: ... And then the DNA fragment encoding 4R1N tau were inserted into the linearized pHR-mCh-Cry2Olig backbone using In-Fusion Cloning Kit (Takara). The resulting constructs were fully sequenced to confirm the absence of unwanted substitutions.
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Physiological activation of the nephron central command drives endogenous kidney tissue regenerationbioRxiv - Physiology 2021Quote: ... Libraries were simultaneously prepared using Takara’s SMARTer Stranded Total-RNA Pico v2 library preparation kit following manufacturer’s protocol (Takara cat#634412). Prepared libraries were sequenced on Illumina Nextseq500 at 2×75cycles.
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bioRxiv - Microbiology 2021Quote: ... 72 °C 20 seconds for 35 cycles followed by subcloning into the eukaryotic expression vector pCAGGs using the In-Fusion HD cloning kit (Takara). All plasmids were midiprepped using the Nucleobond Xtra midi kit (Macherey-Nagel) ...
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Q-SNARE Syntaxin 7 confers actin-dependent rapidly replenishing synaptic vesicles upon high activitybioRxiv - Neuroscience 2020Quote: ... they were cloned into a SmaI site located downstream of the TRE sequence in pLenti6PW–TRE using an In–Fusion Cloning Kit (Clontech)(Egashira et al ...
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bioRxiv - Biophysics 2021Quote: ... The resulting DNA fragments were gel-extracted and cloned individually into the pBADC3 plasmid using InFusion EcoDry cloning kit (TaKaRa). All genes were fused with a C-terminal StrepII-tag for affinity purification.
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bioRxiv - Neuroscience 2022Quote: ... Ribo-depleted sequencing libraries were prepared with the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (TaKaRa Biotech) following manufacturer instructions and sequenced with Genewiz/Azenta on an Illumina NovaSeq S4 machine using a 2 ξ 150 bp pair-end read configuration to a minimum depth of 40 million reads per sample ...
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bioRxiv - Molecular Biology 2022Quote: Rps29 5’UTR and coding region cDNAs were synthesised using SMART™ RACE cDNA Amplification Kit (Clontech Laboratories Inc. USA). PCR was performed using gene-specific primers (40S 5’ RACE R ...
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bioRxiv - Developmental Biology 2022Quote: ... The picked endogenous IHCs and iOHCs were immediately subjected to reverse-transcription and cDNA amplification using a Smart-Seq HT kit (Cat# 634437, Takara). The post-amplified cDNA (1 ng ...
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bioRxiv - Genetics 2022Quote: ... The sequence of amplified MYSM1 cDNA was verified by Sanger sequencing before subcloning into the pLVX overexpression vector using the In-Fusion cloning kit (Takara). Q5 site directed mutagenesis kit was used to make H656R mutant.
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bioRxiv - Developmental Biology 2022Quote: ... Immunoprecipitated DNA and input control (pooled DNA that did not go through the IP step) were finally processed into sequencing libraries using PrepX DNA Library Kit (400075, Takara) and sequenced using the Illumina platform (NextSeq 500 ...
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bioRxiv - Microbiology 2022Quote: ... These two amplified fragments were inserted into the pBh-EPS vector [46] at the PstI site using the In-FusionHD Cloning Kit (Takara). The resultant plasmid and Bsu36I-digested BmNPV-abb genome DNA [47] were cotransfected into BmN-4 cells using FuGeneHD (Promega) ...
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bioRxiv - Neuroscience 2022Quote: The genomic location of the insertion in the enhancer-trap line Et(gata2:EGFP)bi105 was mapped using a linker-mediated approach with the Universal Genome Walker™ 2.0 Kit (Clontech). A detailed user manual is available online ...
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bioRxiv - Neuroscience 2022Quote: Total RNA from mouse cochleae was extracted using an RNA Extraction Kit according to the manufacturer’s instructions (#9767, TaKaRa, Japan). The extracted RNA concentration was measured using a Nanodrop 2000 spectrophotometer (#ND-LITE ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 RNA quantification was performed by RT-qPCR targeting the S gene of SARS-CoV-2 using One Step PrimeScript RT-PCR Kit (Takara) with the following SARS-CoV-2 specific primers and probes ...
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bioRxiv - Developmental Biology 2022Quote: ... was used to purify RNA and 500 ng RNA was used for reverse transcription with a Prime Script RT Reagent Kit (RR037A; Takara). Quantitative PCR was performed using a TB Green Premix DimerEraser Kit (RR091A ...
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bioRxiv - Genetics 2022Quote: ... Reverse transcription was performed using the oligo(dT) primer and avian myeloblastosis virus reverse transcriptase contained in the TaKaRa RNA PCR kit (TaKaRa). RT-qPCR was performed using the Kapa SYBR FAST qPCR kit (Kapa Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... reverse transcription and cDNA preamplification was performed using the SMARTer Ultra Low RNA Kit for the Fluidigm C1 System (Clontech) according to Fluidigm’s manual for mRNA sequencing on the C1 system ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was prepared from cortical samples from control and cKO animals (3 animals/group) by using RNAiso-plus kit (Takara) and according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... with primers containing 15 bp tails homologous to cloning sites within the pGL4.10[luc2] vector which is necessary for cloning using the In-Fusion® HD cloning kit (Takara). Primer sequences are indicated in Table S1 ...
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bioRxiv - Microbiology 2022Quote: ... A total of 500 ng RNA was reverse transcribed into complementary DNA (cDNA) using a PrimeScript II 1st Strand cDNA Synthesis kit (TaKaRa) or a QuantiNova Reverse Transcript Kit (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... the GST gene from the pEGKG plasmid (29) was amplified by PCR and inserted immediately upstream of GFPS65T using the In-Fusion HD cloning kit (Takara).
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bioRxiv - Physiology 2022Quote: ... and cDNA libraries were generated using SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Takara Bio, Inc., Kusatsu, Japan). Libraries were high-output single-end sequenced on a NextSeq500 instrument (Illumina ...
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bioRxiv - Genomics 2019Quote: ... which was further reverse transcribed into cDNA using a PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa, Tokyo, Japan), following the manufacturer’s instructions ...
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bioRxiv - Pathology 2020Quote: ... 3’-RACE-PCR was performed as per the instructions of SMARTer™ RACE-cDNA Amplification Kit User Manual (Clontech, 2012) using primers given in Table 1 ...
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bioRxiv - Immunology 2019Quote: ... Purified RNA was submitted to the Duke Sequencing and Genomic Technologies core facility for library preparation using the Clontech SMARTer v4 Ultra-Low kit (Takara). RNA-seq libraries were sequenced on either an Illumina HiSeq4000 flow cell as 50 bp single-end reads (for Foxp3YFP-cre ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA was extracted from E8.5 trunk tissue (two wild-type trunks and two Aldh1a2-/- trunks) and DNA sequencing libraries were prepared using the SMARTer Stranded Total RNA-Seq Kit v2 Pico Input Mammalian (Takara). Sequencing was performed on Illumina NextSeq 500 ...
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bioRxiv - Cell Biology 2019Quote: ... We then generated by PCR a Linker+ GFP/mCherry which we inserted into the dyn2 vector with the In-Fusion HD kit (Clontech). Dyn2-GFP-ΔCter and Dyn2-GFP-ΔPRD were generated introducing a stop codon at positions 807 (after motif A ...
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bioRxiv - Cancer Biology 2019Quote: ... The cDNA was then ligated into pCDH-CMV-MCS-EF1-GFP using Takara ligation Kit (Clontech, Mountain View, California, USA) and was confirmed in the expression vector by DNA sequencing at The Centre for Applied Genomics (SickKids ...
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bioRxiv - Plant Biology 2020Quote: ... Total amount of 1 μg RNA was used as template for first-strand cDNA synthesis with the oligo(dT) primer and the PrimeScriptTMRT reagent Kit with gDNA Eraser (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... 20 cells were sorted directly in cDNA synthesis buffer from the SMARTer Ultra Low RNA Kit for Illumina sequencing (Clontech) and used directly for cDNA synthesis ...
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bioRxiv - Molecular Biology 2019Quote: ... which were then cloned in the appropriate order into a pUC19 plasmid using an In Fusion HD Cloning Kit (Takara). The substrates were amplified using Phusion Polymerase PCR (NEB) ...
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bioRxiv - Cancer Biology 2019Quote: ... was excised and inserted in frame into a pcDNA3 vector containing fireflluciferase cDNA followed by a T2A sequence using the Infusion HD-eco dry kit (Clontech), as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... and then was used for generating the first strand complementary DNA (cDNA) as described in the protocol of the Takara PrimeScript RT reagent Kit with gDNA Eraser (Takara). Briefly ...