Labshake search
Citations for Takara Bio :
3251 - 3300 of 5201 citations for Human Luteinizing Hormone Beta Polypeptide LHb CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... RNA was then reverse transcribed into cDNA by PrimeScript RT reagent Kit with gDNA Eraser (Takara Bio Inc.). Gene mRNA levels were determined using SYBR green dye on Applied Biosystems Q6 Real-Time PCR cycler (Thermo ...
-
bioRxiv - Biochemistry 2021Quote: The mutant cDNAs of bovine rhodopsin (accession no. AB062417) were constructed using an In-Fusion cloning kit (Clontech). The wild-type and mutant cDNAs of bovine rhodopsin were inserted into the mammalian expression vector pUSRα 24 or pCAGGS 25 ...
-
bioRxiv - Immunology 2022Quote: ... cDNA generation and amplification were performed with SMART-Seq v4 ultra Low Input RNA kit (Takara Bio 634891). Amplified cDNA was purified using AMPure XP Beads (Beckman Coulter A63881) ...
-
bioRxiv - Plant Biology 2021Quote: ... one microgram of DNase-treated RNA was used for reverse transcription using a PrimerScript RT reagent kit (Takara) and a mix of oligo poli-dT and random hexamers ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... cDNAs were synthesized using the SMARTer RACE cDNA Amplification Kit (Cat. No. 634858; Takara, Mountain View, CA, USA). In silico predicted pou-iv gene sequence was retrieved from the Joint Genome Institute genome database (Nematostella vectensis v1.0 ...
-
bioRxiv - Cancer Biology 2021Quote: ... lncRNA NEAT1 and MMP2 mRNA were reversed transcribed into cDNA using PrimeScript RT Reagent Kit (Takara, Dalian, China), and miR-1299 was reversed transcribed into cDNA using TaqMan microRNA Reverse Transcription Kit (Invitrogen ...
-
bioRxiv - Genomics 2020Quote: First strand cDNA synthesis was performed on ~1μg RNA using the SMARTer PCR cDNA Synthesis Kit (Clontech, UK) followed by PCR amplification with PrimeSTAR GXL DNA Polymerase (Clontech ...
-
bioRxiv - Plant Biology 2021Quote: ... Extracted RNA was reverse transcribed using a PrimeScript RT-PCR kit with DNase I (Perfect Real Time) (Takara) in accordance with the accompanying protocol ...
-
bioRxiv - Immunology 2021Quote: ... Full-length cDNA was generated using the Clontech SMARTer v4 kit (Takara Bio USA, Inc., Mountain View, CA) prior to library generation with the Nextera XT DNA Library Prep kit (Illumina ...
-
bioRxiv - Immunology 2020Quote: ... and purification of cDNA was performed using either the SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio USA ...
-
bioRxiv - Immunology 2020Quote: cDNA from sorted cells was generated using the SMART-Seq v4 Ultra low input RNA kit (Clontech # 634890) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... cDNA was generated from 110 ng of total RNA using the PrimeScript™ RT reagent Kit (Takara, Japan) with a combination of both oligo-dT and random hexamers following manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: Samples were prepared with SMARTer® Stranded Total RNA-Seq – Pico Input Mammalian library preparation kit v2 (Takara). Protocol included cDNA synthesis from mRNA ...
-
bioRxiv - Immunology 2020Quote: ... cDNA was generated from 110 ng of total RNA using the PrimeScript™ RT reagent Kit (Takara, Japan) with a combination of both oligo-dT and random hexamers following manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2021Quote: ... and was reverse transcribed using a PrimeScript ® RT reagent kit with gDNA Eraser (RR047A; TaKaRa, Dalian, China). qRT-PCR was performed with SYBR Green master mix (DRR420A ...
-
bioRxiv - Cell Biology 2021Quote: ... cDNA products were amplified by qPCR using the SYBR®Premix Ex Taq TM kit (Takara, RR420A, Japan) in an Applied Biosystem 7500 Fast Real-Time PCR System under standard PCR conditions ...
-
bioRxiv - Genetics 2019Quote: RNA-seq libraries were prepared from 1 ng of total RNA using the SMART-Seq HT Kit (Takara) combined with Nextera XT kit (Illumina) ...
-
bioRxiv - Genomics 2021Quote: We then performed first-strand synthesis using the SMARTer Stranded Total RNA Kit V2-Pico Input Mammalian (Takara). Following purification with RNAclean XP (Beckman Coulter) ...
-
bioRxiv - Genetics 2020Quote: ... and reverse transcription (RT) was performed using PrimeScript RT reagent Kit with gDNA Eraser (TAKARA Bio, Kusatsu, Japan). Primers were designed to specifically amplify cDNA from transfected vectors and endogenous RPGR from 293T and ARPE-19 cells respectively ...
-
bioRxiv - Neuroscience 2020Quote: ... Target sites were amplified from tail genomic DNA by PCR using the Ex Taq Polymerase kit (Takara RR001A) with primers listed in Supplemental Table 2 ...
-
bioRxiv - Cancer Biology 2020Quote: ... cDNA libraries were prepared using SMARTer kit (Clontech Laboratories, Inc. A Takara Bio Company, Mountain View, CA, USA) followed by the Nextera protocols (Illumina ...
-
bioRxiv - Developmental Biology 2022Quote: Rapid amplification of cDNA end (RACE) was performed using the SMARTer® RACE 5’/3’ Kit (Takara, #634858). 1ug of freshly isolated RNA from E8.5 embryo hearts was used to generate first strand cDNA according to the manufactural protocol ...
-
bioRxiv - Biochemistry 2022Quote: ... Five hundred nanogram of total RNA was reverse transcribed by PrimeScript RT reagent kit with gDNA eraser (Takara), and qRT-PCR was performed using KAPA SYBR FAST Master Mix (2X ...
-
bioRxiv - Biochemistry 2022Quote: shTnsB mutants (Fig. 7) were generated in the pHelper vector using the In-Fusion cloning kit from Takara and primers containing the desired point mutations ...
-
bioRxiv - Cancer Biology 2022Quote: ... while 143b-HOS-GFP libraries were prepared using the SMART-Seq v4 Ultra Low Input RNA Kit (Takara). All libraries were sequenced paired-end 100/150bp.
-
bioRxiv - Cell Biology 2022Quote: ... The sequencing library was prepared using the SMART-Seq v4 Ultra Low Input RNA kit (Takara, Kusatsu, Japan), and mRNA-seq on an Illumina NovaSeq 6000 (paired-end ...
-
bioRxiv - Cell Biology 2022Quote: ... PCR products were then isolated through gel electrophoresis and purified using the Nucleospin gel purification kit (Takara Bio). Linear DNA fragments were then ligated using inFusion assembly and amplified in Stellar competent E ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cloning was carried out using the ligation-independent In-Fusion® HD cloning kit (Takara Bio USA, CA). Lentivirus production and transduction were done as described 33 ...
-
bioRxiv - Genetics 2022Quote: ... the target fragment of GmXTH1 was amplified according to the SYBR Premix Ex TaqTM Kit (Takara, Otsu, Japan) operation instructions ...
-
bioRxiv - Microbiology 2022Quote: cDNA was synthesized from total RNA or immunoprecipitated RNA using PrimeScript RT reagent Kit with gDNA Eraser (Takara). RT-qPCR analysis was performed using TB Green Fast qPCR Mix (Takara ...
-
bioRxiv - Microbiology 2022Quote: ... The genes were then cloned into linearized pCMVR backbone using In-Fusion HD Cloning Kit Master Mix (Clontech). Antibodies were produced in Expi293F cells (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... qRT-PCR was performed using the One Step TB Green PrimeScript PLUS RT-PCR Kit (RR096A, TaKaRa, Japan) and a Thermal Cycler Dice Real Time System TP800 (TaKaRa ...
-
bioRxiv - Plant Biology 2022Quote: ... 3XHA or 3XFLAG fragments were inserted into the vector using HindIII sites and the In-Fusion kit (Clontech) to generate basic and control effector vectors p35S::HA or p35S::FLAG ...
-
bioRxiv - Neuroscience 2022Quote: ... libraries were prepared using the SMARTer® Stranded Total RNA-seq Kit v2 – Pico Input Mammalian (Takara, #634413). Samples were fragmented for 4 min @ 94°C and final amplification was performed using 16 cycles ...
-
bioRxiv - Molecular Biology 2023Quote: ... and RNA-sequencing libraries were made using the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Takara). Libraries were sequenced at the Bauer Core Facility (Harvard University ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were sorted directly into buffer for SMART-Seq v4 Ultra Low Input RNA Kit (Clontech Laboratories, Inc.). Subsequent processing was carried out by the UCL Genomics sequencing facility ...
-
bioRxiv - Physiology 2022Quote: ... rRNA depleted RNA passing quality control was used in the SMARTer Stranded RNA-Seq Kit (Takara Bio USA). Following RNA incubation with stranded N6 primer ...
-
bioRxiv - Neuroscience 2022Quote: ... RNA-seq libraries were generated using the SMARTer Stranded Total RNA-seq kit v2 (Takara Cat no. 634419) according to the manufacturer’s instructions ...
-
bioRxiv - Physiology 2022Quote: ... Media lactate dehydrogenase (LDH) was assayed by enzymatic colorimetric assay (LDH Cytotoxicity Detection Kit, Takara Bio, cat# MK401).
-
bioRxiv - Molecular Biology 2022Quote: ... RNA was reverse-transcribed using a PrimeScript RT reagent Kit with gDNA Eraser (Cat: RR047A, TAKARA, Shiga, Japan), and qPCR was performed on a Bio-Rad CFX96 machine (Hercules ...
-
bioRxiv - Cancer Biology 2022Quote: ... USA).and converted to cDNA using a Primescript RT reagent kit (Cat# RR037A, DSS Takara Bio India Private Ltd) as per the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2022Quote: ... The PCR product thus obtained was cloned using the In-Fusion HD Cloning Plus kit (Takara Bio – 638909) into the pMA_F3NeoLoxPPolyFRT vector provided by Dr ...
-
bioRxiv - Cancer Biology 2022Quote: ... The PCR products were purified from agarose gels using NucleoSpin Gel and PCR Clean-Up Kit (740609, Clontech) and subcloned into the pENTR/D-TOPO vector (K240020 ...
-
bioRxiv - Plant Biology 2024Quote: ... Reverse transcription reactions were performed using the PrimeScript II 1st strand cDNA Synthesis Kit (TAKARA, Cat. No. 9750), solution was then removed ...
-
bioRxiv - Neuroscience 2024Quote: ... Libraries were prepared using the low-input Clontech SMART-Seq HT with Nxt HT kit (Takara Bio USA) and sequenced using an Illumina NovaSeq 6000 with an S1 100 Cycle Flow Cell (v1.5).
-
MALAT1 mediates miR-206/CDC42/PAK1/Paxillin signaling axis to alleviate erectile dysfunction in ratsbioRxiv - Molecular Biology 2024Quote: ... And the obtained RNA was used for reverse transcription using the PrimeScript RT Enzyme Mix I kit (TaKaRa). After the reaction was completed ...
-
bioRxiv - Plant Biology 2024Quote: Single or multiple DNA fragments were cloned into binary vector using In-Fusion HD Cloning Kit (Clontech, USA). Briefly ...
-
bioRxiv - Plant Biology 2024Quote: ... and inserted into BamHI-digested pGEX-4T-3 by using In-Fusion Smart Assembly Cloning Kit (Takarabio-Clontech). Full-length OcKSL4 cDNA was amplified by RT-PCR using primers 5’-GGATCCATGGCGAATTATCCCATGGAG-3’ (forward ...
-
bioRxiv - Synthetic Biology 2024Quote: ... One µg of total RNA was processed using PrimeScript RT reagent Kit with gDNA Eraser (Takara, Beijing, China). Amplified products were sequenced and verified after cloning into a pJET1.2-T vector through CloneJET PCR Cloning Kit (Thermo Scientific ...
-
bioRxiv - Zoology 2024Quote: ... One µg of total RNA was processed using PrimeScript RT reagent Kit with gDNA Eraser (Takara, Beijing, China). The PCR procedure was set as follows ...