Labshake search
Citations for Takara Bio :
3101 - 3150 of 5243 citations for SpectraDye Antibody Labeling Kit IR700 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... RNA was then amplified using SMART-Seq v.4 Ultra Low Input RNA kit (Clontech, cat. 63488). Subsequently ...
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bioRxiv - Molecular Biology 2023Quote: ... and cDNA was synthesized using PrimeScript RT reagent Kit (Perfect Real Time, TAKARA BIO Inc. Shiga, Japan). The mouse aorta was homogenized by TissueLyser II (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: ... and SHANK3-3D (T551D/S694D/S781D) were generated with a PrimeSTAR Mutagenesis Basal Kit (Takara, Shiga, Japan). pGEX-14-3-3ζ ...
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bioRxiv - Cancer Biology 2023Quote: Sequencing libraries were generated by SMARTer Stranded Total RNAseq Kit – Pico Input Mammalian V3 (Takara Bio, #634485) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... cDNAs were prepared with Clontech Smart-seq v4 ultra-low kit (Cat# 634888, Takara Bio, Shiga, Japan). PolyA tail enrichment was done using NEBNext® Poly(A ...
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bioRxiv - Microbiology 2023Quote: ... First-strand cDNA was prepared using the Prime Script RT Reagent Kit (Takara Bio, Shiga, Japan; RR047B) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... using the Takara One Step TB Green PrimeScript™ RT-PCR kit (Takara Bio Inc., Shiga, JP) and the Roche LightCycler® 96 real-time PCR instrument (Roche Applied Science ...
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bioRxiv - Microbiology 2023Quote: ... Resulting linear amplification products were pooled and purified using the NucleoSpin Gel and PCR Purification kit (Takara) with modified protocols for ssDNA (1:2 NTI buffer dilution) ...
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bioRxiv - Immunology 2023Quote: ... and cDNA was prepared using the SMART-Seq Ultra Low Input RNA Kit for Sequencing (Takara Bio). RNA-seq libraries were constructed using the NexteraXT DNA Library Preparation Kit (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... cDNA reactions were performed according to the manufacturer’s instructions of the PrimeScript™ RT Reagent Kit (Takara) using primers specifically targeting the U6 and scaffold region (forward primer 5’-CCGACTCGGTGCCACTTTTT-3’ ...
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bioRxiv - Genetics 2023Quote: ... The PCR produc t was purified with a PCR purification kit (Takara Biomedical Technology, Bei jing, China) and was cloned into a pMDTM18-T vector (Takara Biomedical Tec hnology ...
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bioRxiv - Cancer Biology 2023Quote: mRNA was isolated and cDNAs were synthesized by using a Prime Script RT Reagent Kit (TaKaRa, China) according to manufacturer’s instruction ...
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bioRxiv - Cell Biology 2023Quote: RNASequencing libraries were generated using the SMARTer Stranded Total RNA-Seq v2 – Pico Input Mammalian kit (Takara). 100ng of RNA was used as input following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... and RNA-seq libraries were generated using the SMARTer ThruPLEX DNA-Seq Kit (Takara Bio, Cat. # R400674). Each library was indexed using the DNA Unique Dual Index Kit (Takara Bio ...
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bioRxiv - Cell Biology 2023Quote: All viruses and lentiviruses were produced by transfecting Hek293T cells using CalPhos Mammalian Transfection Kit (Takara Bio) according to the manual in DMEM ...
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bioRxiv - Cancer Biology 2023Quote: These RNA samples were used for cDNA synthesis using PrimeScript 1st strand cDNA synthesis kit (Takara, Japan) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... by replacing EGFP-coding sequences with mCherry-coding sequences using In-Fusion® HD Cloning Kit (Clontech).
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bioRxiv - Immunology 2023Quote: ... cDNA libraries were constructed using the SMARTer Stranded Total RNA-Seq Kit (v2) – Pico Input Mammalian (Clontech) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... Full-length cDNA was generated using the TaKaRa SMART-Seq v4 Ultra Low kit (TaKaRa Cat # 634889). mRNA-Seq libraries were then prepared using the Illumina Nextera XT DNA Library Preparation kit (Illumina Cat # FC-131-1024) ...
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bioRxiv - Evolutionary Biology 2023Quote: The SMARTSeq HT Ultra Low Input Kit was used for full-length cDNA synthesis and amplification (Clontech), and Illumina Nextera XT library was used for sequencing library preparation ...
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bioRxiv - Microbiology 2023Quote: ... DNA in the supernatant was purified using the NucleoSpin Gel & PCR kit (Takara Bio, Kusatsu, Shiga, Japan). Sequencing libraries were prepared from 3 ng DNA with the Kapa HyperPrep Kit (Roche ...
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bioRxiv - Genetics 2024Quote: ... Complementary DNA (cDNA) was synthesized from total RNA using a PrimeScript RT reagent Kit (Cat#RR047B, TaKaRa) and was used as a template to perform qPCR in TB green-based qPCR instrument (CFX-96 ...
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bioRxiv - Bioengineering 2024Quote: ... RNA was isolated through a NucleoSpin® RNA/Protein kit (Takara Bio USA Inc. San Jose, CA) while heart issue was processed with a RNeasy Fibrous Mini kit (Qiagen ...
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bioRxiv - Molecular Biology 2024Quote: ... The digested backbone and the two amplicons were joined by In-Fusion® HD Cloning Kit (Takara). The SFFV U3 in the pAS.SFFV.d2GFP.PRE plasmid was then exchanged for other U3 sequences by digesting the plasmid with XcmI and AscI (NEB ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA sequencing libraries were prepared using SMARTer Stranded Total RNA-Seq Kit v3 (Takara Bio USA, Inc) following the recommended protocol with minor adjustments ...
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bioRxiv - Cell Biology 2024Quote: ... the first strand cDNA was prepared using PrimeScript II 1st Strand cDNA Synthesis Kit (Takara, Beijing, China).
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bioRxiv - Cell Biology 2024Quote: ... Quantitative real-time polymerase chain reaction (qPCR) was performed using TB Green Premix Ex Taq Kit (Takara) on QuantStudio 6 Flex Real-Time PCR System (Life Technologies) ...
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bioRxiv - Biophysics 2024Quote: ... and cDNA was synthesized using PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and cDNA was synthesized from 1 μg RNA using PrimeScriptTM RT Master Mix kit (Takara, Cat#RR036A) following the manufacturer’s protocols ...
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bioRxiv - Bioengineering 2024Quote: ... We confirmed the cells were mycoplasma-negative using a Cycleave PCR Mycoplasma Detection Kit (Takara Bio, CY232).
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bioRxiv - Neuroscience 2024Quote: ... immediately followed by reverse-transcriptions and cDNA amplification with the smart-seq HT kit (Cat# 634437, Takara). The post-amplified cDNAs (1 ng per sample ...
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bioRxiv - Microbiology 2024Quote: ... 5′ RACE was carried out using SMARTer RACE 5′/3′ Kit (Takara Bio USA, Mountain View, CA) to identify the transcription start site (TSS ...
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bioRxiv - Microbiology 2024Quote: The processed DNA was ready for Illumina library preparation using a SMART ChIP-seq kit (Takara, #634865) following the manufacturer’s protocol (Fig ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA (1 μg) was reverse transcribed into cDNA using PrimeScript RT reagent kit (Takara Bio, Japan), and expression was quantified using the TB Green® Premix Ex Taq™ II (Takara) ...
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bioRxiv - Cell Biology 2024Quote: ... we amplified EF1a promoter region from pEP4 E02S CK2M EN2L by PCR and cloned into pHR_SFFV_LaG17_synNotch_TetRVP64 using infusion cloning kit (Takara, 638947). After packaging the lentivirus ...
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bioRxiv - Cell Biology 2024Quote: ... These fragments were cloned into pHAGE-EF1a-Cre-w41 plasmid using an infusion cloning kit (Takara, 638947). Lentiviral vectors were transfected into the packaging HEK293 cell line ...
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bioRxiv - Genetics 2024Quote: ... cDNA was generated from 1μg of total RNA using the PrimeScriptTM RT reagent kit (TaKaRa, Shiga, Japan) for the quantitation of rRNA by quantitative real-time PCR ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cDNA was reverse transcribed by using a PrimeScript RT Reagent Kit Perfect Real Time (TaKaRa Bio Inc.). Real-time PCR was performed using a 7500 Fast Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Cell Biology 2024Quote: ... The lentiviral titer was then determined using the Lenti-X p24 rapid titer kit (Takara Cat #632200). The Tau-RD-P301S-K18 FRET Biosensor CRL-3275 were exposed to active recombinant P301S-MAPT fibrils (ab246003 ...
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bioRxiv - Cell Biology 2024Quote: Paraffin sections were used for H/E staining and ALP staining with a commercial staining kit (TaKaRa, TRACP & ALP double-stain kit ...
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bioRxiv - Cell Biology 2019Quote: ... for 30 min at 37°C in a moisture chamber followed by an incubation with anti-GFP (1:100 in PBSA) antibody at 4°C for 72 h (Takara 632381/JL-8)) ...
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bioRxiv - Neuroscience 2020Quote: ... 16 μm in thickness) were processed for immunodetection of Mouse anti-GFP antibody (1:500, 632381, Takara Bio USA, Inc. Mountain View, CA) and incubated with secondary Donkey anti-Mouse Alexa Fluor 488 (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were directed against IBA1 (rabbit-anti-iba1 WAKO chemicals 019-19741 1:1000) and tdTomato (mouse-anti-dsRed Takara 632392 1:1000). Secondary antibodies were goat anti-rabbit or anti-mouse conjugated with Alexa dyes 488 and 568 (1:1000) ...
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bioRxiv - Physiology 2019Quote: ... Membranes were incubated at 4°C in primary antibodies diluted 1:1000 in 5% bovine serum albumin: anti-GFP (ClonTech Living Colours #ab632375), anti-HSP60 (Department of Biology ...
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bioRxiv - Neuroscience 2024Quote: ... The primary antibody used was rabbit-anti-dsRed (1:500, Living Colors DsRed pAb, RRID: AB_10013483; Takara Bio USA, Mountain View, CA, USA) to enhance mCherry-labeled rVRG axon visualization ...
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bioRxiv - Neuroscience 2023Quote: ... the brain slices were first blocked in 10% goat serum made in 0.1% Triton X-100/1× PBS and then incubated in anti-GFP antibodies (1/1000, RT, overnight, Mouse anti-GFP, 632381, Takara Bio USA, WI) followed by Alexa-488 secondary antibodies (1/200 ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were incubated in primary antibody (chicken anti-GFP, Abcam #13970, 1:2000, rabbit anti-DsRed, Takara Bio Clontech #632496, 1:200) for 24 – 48 hours in the dark while shaking at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were incubated in primary antibody (chicken anti-GFP, Abcam #13970, 1:2000, rabbit anti-DsRed, Takara Bio Clontech #632496, 1:200) for 24 – 48 hours in the dark while shaking at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Tissues were rinsed four times for 15 min each with 0.1 M PBS and incubated with secondary antibodies anti-DsRed (1:500; RRID:AB_10013483; Living Colors DsRed polyclonal; Takara Bio USA, San Jose, CA) and anti-Troma1 (1:500 ...
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bioRxiv - Synthetic Biology 2021Quote: ... DmrA and DmrC were amplified and then fused to each split-Cas9 (N_Cas9 and C_Cas9) using the In-Fusion HD Cloning Kit (Clontech). Links to sequences of all the generated plasmids are listed in Supplementary Table 3.