Labshake search
Citations for Takara Bio :
3351 - 3400 of 5243 citations for SpectraDye Antibody Labeling Kit IR700 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... The two fragments were assembled in a single In-Fusion reaction (In-Fusion HD Cloning Kit, Takara, 639650), and the PCR-derived regions of the resulting plasmids were confirmed by sequencing.
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bioRxiv - Cell Biology 2024Quote: Total RNA was isolated with Trizol (Thermo) and cDNA was synthesized with Prime Script RT Reagent Kit (Takara). Quantitative PCR was performed with a SYBR Green PCR kit (Toyobo ...
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bioRxiv - Pathology 2023Quote: ... Reverse RNA transcription was performed using the PrimeScript RT Reagent Kit with gDNA Eraser (RR047A; Takara Bio, Japan). A 10 μL reaction mix was formulated as follows ...
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bioRxiv - Cell Biology 2022Quote: ... and in-chip reverse transcription PCR were performed using a 3’ DE Chip and Reagent kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... Plasmids coding EGFP-mutants were generated from EGFP-IRES-mCherry plasmid using TaKaRa MutanBEST Kit (TaKaRa, Kusatsu, Japan). pG5egfp reporter vector was modified from the pG5luc vector (GeneBank Accession Number AF264724 ...
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bioRxiv - Cell Biology 2023Quote: ... and used for library preparation (5-10 ng) using SMART-Seq v4 Ultra Low Input RNA Kit (Clontech). Libraries were sequenced on Novaseq platform (Illumina).
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bioRxiv - Cell Biology 2023Quote: ... RNA-seq libraries were prepared with the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Takara #634875). 75 bp single-end sequencing was performed using an Illumina NextSeq500 system at the CRI at UT Southwestern Sequencing Facility ...
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bioRxiv - Immunology 2023Quote: ... and 1 µg was used for cDNA synthesis with the PrimeScript High Fidelity RT-PCR Kit (Takara, R022B). Of the cDNA ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR product was gel purified using the Nucleospin Gel and PCR Clean-Up Kit (TaKaRa Bio Inc.) and cloned in between the XhoI and SalI sites of psiCHECK-2.2 using InFusion cloning (TaKaRa Bio Inc.) ...
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bioRxiv - Developmental Biology 2023Quote: ... Purified total RNA was reverse transcribed into cDNA by PrimeScript II 1st strand cDNA Synthesis Kit (Takara, 6210A) or SMARTer™ RACE cDNA Amplification Kit (Clontech) ...
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bioRxiv - Developmental Biology 2023Quote: ... Polyadenylated RNA-seq libraries were prepared by using SMART-Seq v4 PLUS Kit (Clontech, Mountain View, CA, USA). Indexed RNA-seq libraries were sequenced using Illumina HiSeqX sequencer (paired end ...
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bioRxiv - Synthetic Biology 2023Quote: ... Specific primers were designed for this purpose and PCR was done using the Advantage 2 Polymerase kit (Clontech). Complementary 60 bp-ends to each target sequence on all Mycoplasmas species genome used in this study ...
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bioRxiv - Plant Biology 2023Quote: ... The double-strand oligonucleotides containing the target sequences were synthesized and cloned into pMpGE_En01 or pMpGE_En03 using In-Fusion HD cloning kit (TaKaRa) and BsaI restriction sites in pMpGE_En03 ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA was synthesized using 1 µg of RNA following manufacturers’ instruction (1st strand cDNA synthesis kit, Takara; 6110A). RT-PCR was performed using SapphireAmp® Fast PCR Master Mix (Takara ...
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bioRxiv - Plant Biology 2023Quote: ... SRF mutants were prepared using PrimeSTAR Mutagenesis Basal Kit according to the manufacturer’s instructions (TaKaRa Bio, Shiga, Japan), and the mutations were confirmed by sequencing ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Complete mtDNA was amplified in several overlapping fragments using the Long and Accurate (LA) PCR kit from TAKARA.
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bioRxiv - Cell Biology 2023Quote: ... synthesized double strand DNA of BrUSLEE-SKL was cloned to pT1O using In-Fusion HD Cloning Kit (Takara). DNA fragments of mCherry-SKL ...
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bioRxiv - Molecular Biology 2023Quote: ... The amplified fragments and vector pBFv-UAS3 were fused using the In-Fusion® HD Cloning Kit (TaKaRa). The oligonucleotide sequences used are shown in Supplementary Table 2 ...
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bioRxiv - Plant Biology 2023Quote: ... and cloned into the XbaI digestion site of pMpGWB307 vectors using In-Fusion HD Cloning Kit (Takara Bio) to produce pMpGWB307-MpJINp ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR products were cut from agarose gels and purified using the Nucleospin gel purification kit (Takara Bio, 240609). Plasmids were assembled from linear fragments using In-Fusion HD (Takara Bio ...
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bioRxiv - Physiology 2023Quote: Total RNA was extracted from tissues using the RNAiso Plus kit and treated with DNase I (TaKaRa, China). The purified RNA was used for first-strand cDNA synthesis ...
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bioRxiv - Microbiology 2023Quote: ... Purified RNA was quantified and used for cDNA preparation using the PrimeScript 1st strand cDNA Synthesis Kit (Takara) with 1 μg of extracted RNA ...
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bioRxiv - Plant Biology 2023Quote: ... after which first-strand cDNA was synthesized from total RNA using a cDNA reverse transcription kit (TaKaRa, Japan). Full-length CDS sequences of the three candidate UGT genes were obtained from third-generation transcriptome sequencing data (Chen et al ...
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bioRxiv - Plant Biology 2023Quote: ... RNA (0.5 μg) was reverse transcribed by the PrimeScript RT reagent kit with genomic DNA Eraser (Takara, USA) using random and oligo-dT primer mix in a 10 μL reaction ...
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bioRxiv - Immunology 2023Quote: Reverse transcription and cDNA amplification were performed with the SMART-Seq v4 Ultra Low Input RNA Kit (Takara). Barcoded Illumina-compatible libraries were generated from 5 to 10Lng of DNA of each sample ...
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bioRxiv - Genomics 2023Quote: ... Libraries were prepared using the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Takara Bio USA, Inc.), which incorporates both RiboGone and SMART (Switching Mechanism At 5’ end of RNA Template ...
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bioRxiv - Genomics 2023Quote: ... Illumina sequencing libraries were prepared from ChIP-enriched DNA using ThruPLEX DNA-seq library preparation kit (Takara Bio) with up to 10ng of input DNA and 8-15 PCR cycles ...
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bioRxiv - Genetics 2023Quote: ... The RNA was reverse-transcribed into cDNA by using a Prime ScriptTM RT Reagent Kit (TaKaRa, Kyoto, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR products were cut from agarose gels and purified using the Nucleospin gel purification kit (Takara Bio, 240609). Final plasmids were constructed using In-Fusion HD (Takara Bio ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA fragmentation was performed using In situ Apoptosis Detection Kit according to the manufacturer’s indications (Takara Bio Inc.). For immunostaining ...
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bioRxiv - Cell Biology 2023Quote: ... and N-SREBP2 (1440 bps) were amplified using high-fidelity PCR (Advantage-HF 2 PCR kit, #639123; Clontech) from human pcDNA3.1-2xFLAG-SREBP1a (#26801 ...
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bioRxiv - Immunology 2023Quote: ... Total RNA was normalized prior to oligo-dT capture and cDNA synthesis with SMART-seq v4 kit (Takara). The resulting cDNA was quantitated using a Qubit 3.0 fluorometer ...
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bioRxiv - Immunology 2023Quote: ... linearized BF520 sequence was gel purified using NucleoSpin Gel and PCR Clean-up kit (Takara, Cat. No. 740609.5) and then purified using Ampure XP beads (Beckman Coulter ...
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bioRxiv - Cell Biology 2023Quote: ... and a PrimeScript RTreagent kit with gDNA Eraser and SYBR Premix Ex Taq II (TliRNase H Plus) (TaKaRa) and an ABI7500 qPCR instrument (Life Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... The PCR product was cloned into SalI-PstI-digested pKG120 by In-Fusion HD Cloning Kit (Takara bio). The resulting plasmid ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was prepared using the SMARTer procedure through the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech) for Illumina Sequencing ...
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bioRxiv - Molecular Biology 2023Quote: Library preparation: cDNA was synthesized with the SMARTer Universal Low Input RNA Kit protocol (Clontech, cat. no. 634938) using the standard RiboGone–Mammalian Kit protocol.
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bioRxiv - Developmental Biology 2023Quote: ... 18-20 μg of indicated plasmids were transfected in HEK293T cells using the CalPhos Mammalian Transfection Kit (Takara). After 48h ...
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bioRxiv - Developmental Biology 2023Quote: ... and subcloned into the EcoRI site of pT2A-BItight-TRE by In-Fusion® HD cloning kit (TaKaRa). pT2A-BItight-Lifeact-mCherry-TRE ...
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bioRxiv - Plant Biology 2023Quote: ... and terminator was assembled into the backbone PCB1532 via In-Fusion cloning (In-Fusion cloning kit, Clontech Laboratories).
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bioRxiv - Plant Biology 2023Quote: ... 4 μg of total RNA was used for cDNA synthesis with PrimeScript 1st strand cDNA Synthesis Kit (Takara), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... First strand cDNA synthesis was performed on 200ng RNA using the SMARTer PCR cDNA Synthesis Kit (Clontech, UK) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Libraries were prepared using the SMARTer Stranded Total RNA-Seq kit v2 - Pico Input Mammalian (Clontech, TaKaRa Bio). Unfragmented RNA was reverse transcribed with adapter-linked random hexamers and the first strand cDNA was then converted into double-stranded dual-indexed DNA libraries ...
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bioRxiv - Microbiology 2023Quote: ... 5’RACE was performed with SMARTer RACE 5’/3’ Kit (Takara Bio USA, Inc. San Jose, CA USA) according to the manufacturer’s directions.
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bioRxiv - Cell Biology 2023Quote: ... Sequencing libraries from treated RNA were created using the SMARTer Stranded Total RNA HI Mammalian kit (Takara 634873) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: All libraries were prepared using the SMARTer Stranded Total RNaseq Kit v2 – Pico Input Mammalian Components (634419, Takara) according to manufacturer’s protocols and barcoded (634452 ...
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bioRxiv - Genomics 2023Quote: ... Libraries were prepared using the SMARTer Stranded Total RNA-Seq kit v2 - Pico Input Mammalian (Clontech, TaKaRa Bio). Unfragmented RNA was reverse transcribed with adapter-linked random hexamers and the first strand cDNA was then converted into double-stranded dual-indexed DNA libraries ...
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bioRxiv - Immunology 2023Quote: ... Genomic DNA was isolated from tail biopsies using the NucleoSpin Tissue kit (TaKaRa Bio USA, San Jose, CA) following manufacture protocol ...
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bioRxiv - Microbiology 2023Quote: ... Reverse transcription was performed to make cDNA using PrimeScript RT reagent kit with gDNA Eraser (TaKaRa, Kyoto, Japan). Sequences encoding the C-terminal portion (amino acid 45 to 131 ...
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bioRxiv - Immunology 2023Quote: ... Total RNA was normalized prior to oligo-dT capture and cDNA synthesis with SMART-seq v4 kit (Takara). The resulting cDNA was quantitated using a Qubit 3.0 fluorometer ...