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Citations for Takara Bio :
3001 - 3050 of 5201 citations for Human Luteinizing Hormone Beta Polypeptide LHb CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... Total RNA was used for cDNA synthesis (PrimeScript RT Reagent Kit; Takara Bio, Takara Bio, Kusatsu, Japan). Transcript expression was quantified by real-time qPCR using iQ SYBR Green super mix (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2023Quote: ... Total RNA was used for cDNA synthesis (PrimeScript RT Reagent Kit; Takara Bio, Takara Bio, Kusatsu, Japan). Transcript expression was quantified by real-time qPCR using iQ SYBR Green super mix (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2022Quote: ... the corresponding cDNA was transcribed using a Fast Quant RT Kit with gDNA (TaKaRa Bio, Shiga, Japan). The cDNA was used as a template for RT-PCR assays ...
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bioRxiv - Molecular Biology 2022Quote: ... the total RNA was reverse transcribed to cDNA using the Prime ScriptTM RT reagent kit (TaKaRa Biotechnology). qPCR was performed using the SYBR Premix Ex Taq II Kit (TaKaRa Biotechnology ...
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bioRxiv - Molecular Biology 2022Quote: ... The DNA band was cut out and purified using the Nucleospin® Extract II Kit (Clontech Laboratories) protocol ...
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bioRxiv - Plant Biology 2022Quote: ... The downstream genomic region was then amplified using the primer set MpHEC1_CDS_IF_F and MpHEC1_3UTR_IF_R2 and integrated into the first binary plasmid with an In-Fusion HD Cloning Kit (Clontech) using the BamHI and AscI sites ...
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bioRxiv - Microbiology 2022Quote: ... DNA fragments were assembled into pEMC vector one-by-one using in-fusion HD cloning kit (Takara), generating pEMC-eGFP-MBaMV ...
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bioRxiv - Microbiology 2022Quote: ... and used for plasmid DNA preps using the Endotoxin-Free Nucleobond Plasmid Midiprep kit (Takara Bio; 740422.10). The HIVDEP library was sequenced and contains all 4,191 sgRNAs included in the synthesis (GEO Dataset ...
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bioRxiv - Molecular Biology 2022Quote: ... rAAVs were purified using AAVpro® Purification Kit for All Serotypes (Cat # 6666, Takara Bio USA, CA) and each of the purified rAAVs were tested in separate transwell BBB cultures with hCMEC/D3 cells and primary astrocytes at MOI of 1e4 ...
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bioRxiv - Molecular Biology 2022Quote: cDNA was synthesized with 1 μg of total RNA using the Prime ScriptTM RT Reagent Kit (Takara) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and ligated into the digested lentiCRISPRv2 backbone using the DNA ligation kit (Mighty Mix) (Takara, Cat# 6023). The ligated DNA was then transformed into One Shot Stabl3 competent cells (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... The viral titer was measured using a Lenti-X qRT‒PCR Titration Kit (#631235, TaKaRa, Shiga, Japan).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and cDNA was synthesized from the extracted RNA using PrimeScriptTM RT Reagent Kit (Takara Bio Inc., Japan) according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA synthesis was performed using a one-step PrimeScript RT-PCR kit (Takara Bio, Cat. RR057B, Japan) as per the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2024Quote: ... sgRNAs efficiency was assessed in vitro using the sgRNA In vitro Transcription and Screening kit (Clontech, 631439) following the manufacturer’s protocol ...
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Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesisbioRxiv - Systems Biology 2024Quote: ... Linearized pMK1334 vector was purified using NucleoSpin Gel and PCR clean-up kit (Takara Bio, Cat#740609.50). Top and bottom oligos of non-targeting and targeting sgRNAs were annealed ...
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bioRxiv - Neuroscience 2024Quote: ... The target genes were amplified by qPCR according to the instructions of kits (Cat.no. 638315 Takara, Japan), reversely ...
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bioRxiv - Cancer Biology 2024Quote: ... The SMARTSeq HT Ultra Low Input Kit was used for full-length cDNA synthesis and amplification (Clontech), and Illumina Nextera XT library was used for sequencing library preparation ...
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Regulation of Diseases-Associated Microglia in the Optic Nerve by Lipoxin B4 and Ocular HypertensionbioRxiv - Molecular Biology 2024Quote: ... mRNA was converted to cDNA using SMARTer v4 Ultra Low Input RNA Kit (Clontech, Mountain View, CA). A Diagenode Bioruptor Pico was used to fragment the cDNA ...
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bioRxiv - Neuroscience 2024Quote: ... followed by a T7 endonuclease I (T7EI) assay using the Guide-it Mutation Detection Kit (Takara Bio) following the standard protocol for mismatch detection.
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bioRxiv - Cancer Biology 2024Quote: ... RNA libraries were prepared using Smarter Stranded Total RNA-Seq Kit - Pico Input Mammalian (Takara Bio, #635005). For committed cells ...
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bioRxiv - Cell Biology 2024Quote: ... and the YFP cDNA was assembled using an In-fusion HD cloning kit (Takara-Bio, Shiga, Japan). (2 ...
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bioRxiv - Cell Biology 2023Quote: ... the RNA was reverse transcribed using the PrimeScriptII 1st strand cDNA Synthesis Kit (Takara bio, Shiga, Japan). Quantitative RT-PCR was performed using SYBR Premix EX TaqII (Takara bio ...
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bioRxiv - Immunology 2023Quote: ... Illumina libraries were constructed using SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (TaKaRa, Japan). RNA-seq libraries were sequenced at read lengths 2 × 151bp on the Illumina Novaseq 6000 (Illumina) ...
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bioRxiv - Microbiology 2024Quote: ... Each cDNA was generated using a Clontech SMART-Seq HT Kit (Takara Clontech, Mountain View, CA, USA), and each library was prepared using a Nextera XT DNA Library Prep Kit (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... Each cDNA was generated using a Clontech SMART-Seq HT Kit (Takara Clontech, Mountain View, CA, USA), and each library was prepared using a Nextera XT DNA Library Prep Kit (Illumina ...
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bioRxiv - Molecular Biology 2024Quote: ... or 5 ng of total RNA (with a SMARTer Stranded Total RNA-Seq Kit v3; Takara Bio) was used.
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bioRxiv - Genomics 2024Quote: ... Both reagents were included in SMART-Seq Single Cell PLUS kit (Takara Bio, San Jose, CA, U.S.). Immediately after sampling in lysis buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Sequencing libraries were prepared using the SMARTer v4 Ultra Low Input RNA Kit (Clontech, Mountain View, CA). Sequencing was performed on NovaSeq S4 150PE platform (Illumina ...
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bioRxiv - Cell Biology 2023Quote: ... DUSP13 deficient in phosphatase activity (=Dusp13_Mut) was generated using the PrimeSTAR® Mutagenesis Basal Kit (Takara Bio). The Dusp family contains the amino acid cysteine ...
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bioRxiv - Genomics 2024Quote: ... Library construction was performed based on the manufacturer’s recommendation for SmarterStranded V2 kit (Takara Bio, California, USA). The final library quantity was measured by KAPA SYBR FAST qPCR and library quality was evaluated by TapeStation D1000 ScreenTape (Agilent Technologies ...
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bioRxiv - Immunology 2024Quote: ... cDNA libraries were prepared using SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (Takara, Japan) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... The reverse transcription was performed using the PrimeScript RT reagent kit (Takara, Saint-Germain-en-Laye, France) with a primer oligo-d(T)-AP (5’ GACCACGCGTATCGATGTCGACTTTTTTTTTTTTTTTTv 3’) ...
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bioRxiv - Genomics 2024Quote: ... except that an alternative oligo-dT primer from the SMARTer PCR cDNA Synthesis kit (Clontech Laboratories, Inc.), which also included a random 10mer sequence as a unique molecule identifier (UMI ...
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bioRxiv - Biochemistry 2023Quote: The PrimeSTAR® MAX DNA Polymerase and In-Fusion® HD Cloning Kit were purchased from Takara Bio Inc ...
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bioRxiv - Bioengineering 2024Quote: ... Viral genome number was quantified by real-time PCR with AAVpro Titration Kit Ver.2 (Takara, 6233) according to manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: ... Reverse transcription of RNA was carried out using the PrimeScript RT reagent Kit with gDNAEraser from TAKARA. The resulting cDNA from reverse transcription was then amplified by PCR and digested using PstI ...
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bioRxiv - Molecular Biology 2023Quote: ... Then the cDNA was synthesized using PrimeScript RT Reagent Kit with gDNA Eraser (Perfect Real Time) (TaKaRa). Real-time quantitative PCR was performed as previously described [13 ...
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bioRxiv - Microbiology 2023Quote: ... All cloning experiments were performed using an In-Fusion HD Cloning Kit (Takara Bio Inc., Shiga, Japan), and plasmid sequences were confirmed using a DNA sequencing service (Core Instrumentation Facility ...
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bioRxiv - Microbiology 2023Quote: Total RNA of each strain was extracted with the RNA extraction kit (Takara biomedical technology, Beijing, China) following the manufacturer’s instruction ...
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bioRxiv - Genomics 2023Quote: ... The obtained PCR products were purified using NucleoSpin Gel and PCR Cleanup kit (Takara Bio, Shiga, Japan) and sequenced directly or were cloned into the pTAC-1 vector (Biodynamics ...
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bioRxiv - Immunology 2023Quote: ... 500 ng of RNA was used for cDNA synthesis using a PrimeScript RT Reagent Kit (Takara, RR037A). 2 μL of diluted cDNA (1:50 ...
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bioRxiv - Plant Biology 2023Quote: ... Two biological replicates were processed for next-generation library preparation using the ThruPLEX DNA-seq kit (Takara). Next ...
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bioRxiv - Genomics 2023Quote: ... RNA was extracted from the plume of six individuals using RNAiso Pure RNA Isolation Kit (Takara, Japan) and assessed for quality using NanoVue Plus spectrophotometer (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... The PCR mix (15 ul) contained One step TB Green RT-PCR kit (RR096A, Takara Bio Inc.), each primer ...
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bioRxiv - Immunology 2023Quote: ... directly into reaction buffer from the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara), and reverse transcription was performed followed by PCR amplification to generate full length amplified cDNA ...
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bioRxiv - Cell Biology 2023Quote: ... The real-time PCR reaction was performed using One Step SYBR Prime Script RT-PCR Kit (TakaRa). The primers for 14-3-3β ...
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bioRxiv - Cancer Biology 2023Quote: ... bacteria cells were pelleted and BAC DNA was extracted using the NucleoBond Xtra BAC kit (Takara, 740436) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and inserted into either the pcDNA3.1+ or pcDNA3.1_N-eGFP vectors using the In-Fusion HD Cloning Kit (Takara). Following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Quantitative PCR was performed using the Mir-X miRNA qRT-PCR TB Green Kit (Takara Bio, USA) using the protocol provided by the manufacturer ...