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Citations for Takara Bio :
2951 - 3000 of 4900 citations for mu p75 SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... cDNA was generated from 110 ng of total RNA using the PrimeScript™ RT reagent Kit (Takara, Japan) with a combination of both oligo-dT and random hexamers following manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Samples were prepared with SMARTer® Stranded Total RNA-Seq – Pico Input Mammalian library preparation kit v2 (Takara). Protocol included cDNA synthesis from mRNA ...
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bioRxiv - Immunology 2020Quote: ... cDNA was generated from 110 ng of total RNA using the PrimeScript™ RT reagent Kit (Takara, Japan) with a combination of both oligo-dT and random hexamers following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... and was reverse transcribed using a PrimeScript ® RT reagent kit with gDNA Eraser (RR047A; TaKaRa, Dalian, China). qRT-PCR was performed with SYBR Green master mix (DRR420A ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA products were amplified by qPCR using the SYBR®Premix Ex Taq TM kit (Takara, RR420A, Japan) in an Applied Biosystem 7500 Fast Real-Time PCR System under standard PCR conditions ...
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bioRxiv - Genetics 2019Quote: RNA-seq libraries were prepared from 1 ng of total RNA using the SMART-Seq HT Kit (Takara) combined with Nextera XT kit (Illumina) ...
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bioRxiv - Genomics 2021Quote: We then performed first-strand synthesis using the SMARTer Stranded Total RNA Kit V2-Pico Input Mammalian (Takara). Following purification with RNAclean XP (Beckman Coulter) ...
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bioRxiv - Genetics 2020Quote: ... and reverse transcription (RT) was performed using PrimeScript RT reagent Kit with gDNA Eraser (TAKARA Bio, Kusatsu, Japan). Primers were designed to specifically amplify cDNA from transfected vectors and endogenous RPGR from 293T and ARPE-19 cells respectively ...
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bioRxiv - Neuroscience 2020Quote: ... Target sites were amplified from tail genomic DNA by PCR using the Ex Taq Polymerase kit (Takara RR001A) with primers listed in Supplemental Table 2 ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNA libraries were prepared using SMARTer kit (Clontech Laboratories, Inc. A Takara Bio Company, Mountain View, CA, USA) followed by the Nextera protocols (Illumina ...
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bioRxiv - Developmental Biology 2022Quote: Rapid amplification of cDNA end (RACE) was performed using the SMARTer® RACE 5’/3’ Kit (Takara, #634858). 1ug of freshly isolated RNA from E8.5 embryo hearts was used to generate first strand cDNA according to the manufactural protocol ...
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bioRxiv - Biochemistry 2022Quote: ... Five hundred nanogram of total RNA was reverse transcribed by PrimeScript RT reagent kit with gDNA eraser (Takara), and qRT-PCR was performed using KAPA SYBR FAST Master Mix (2X ...
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bioRxiv - Biochemistry 2022Quote: shTnsB mutants (Fig. 7) were generated in the pHelper vector using the In-Fusion cloning kit from Takara and primers containing the desired point mutations ...
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bioRxiv - Cancer Biology 2022Quote: ... while 143b-HOS-GFP libraries were prepared using the SMART-Seq v4 Ultra Low Input RNA Kit (Takara). All libraries were sequenced paired-end 100/150bp.
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bioRxiv - Cell Biology 2022Quote: ... The sequencing library was prepared using the SMART-Seq v4 Ultra Low Input RNA kit (Takara, Kusatsu, Japan), and mRNA-seq on an Illumina NovaSeq 6000 (paired-end ...
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bioRxiv - Cell Biology 2022Quote: ... PCR products were then isolated through gel electrophoresis and purified using the Nucleospin gel purification kit (Takara Bio). Linear DNA fragments were then ligated using inFusion assembly and amplified in Stellar competent E ...
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bioRxiv - Cancer Biology 2022Quote: ... Cloning was carried out using the ligation-independent In-Fusion® HD cloning kit (Takara Bio USA, CA). Lentivirus production and transduction were done as described 33 ...
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bioRxiv - Genetics 2022Quote: ... the target fragment of GmXTH1 was amplified according to the SYBR Premix Ex TaqTM Kit (Takara, Otsu, Japan) operation instructions ...
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bioRxiv - Microbiology 2022Quote: cDNA was synthesized from total RNA or immunoprecipitated RNA using PrimeScript RT reagent Kit with gDNA Eraser (Takara). RT-qPCR analysis was performed using TB Green Fast qPCR Mix (Takara ...
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bioRxiv - Microbiology 2022Quote: ... The genes were then cloned into linearized pCMVR backbone using In-Fusion HD Cloning Kit Master Mix (Clontech). Antibodies were produced in Expi293F cells (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... qRT-PCR was performed using the One Step TB Green PrimeScript PLUS RT-PCR Kit (RR096A, TaKaRa, Japan) and a Thermal Cycler Dice Real Time System TP800 (TaKaRa ...
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bioRxiv - Plant Biology 2022Quote: ... 3XHA or 3XFLAG fragments were inserted into the vector using HindIII sites and the In-Fusion kit (Clontech) to generate basic and control effector vectors p35S::HA or p35S::FLAG ...
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bioRxiv - Neuroscience 2022Quote: ... libraries were prepared using the SMARTer® Stranded Total RNA-seq Kit v2 – Pico Input Mammalian (Takara, #634413). Samples were fragmented for 4 min @ 94°C and final amplification was performed using 16 cycles ...
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bioRxiv - Molecular Biology 2023Quote: ... and RNA-sequencing libraries were made using the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Takara). Libraries were sequenced at the Bauer Core Facility (Harvard University ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were sorted directly into buffer for SMART-Seq v4 Ultra Low Input RNA Kit (Clontech Laboratories, Inc.). Subsequent processing was carried out by the UCL Genomics sequencing facility ...
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bioRxiv - Physiology 2022Quote: ... rRNA depleted RNA passing quality control was used in the SMARTer Stranded RNA-Seq Kit (Takara Bio USA). Following RNA incubation with stranded N6 primer ...
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bioRxiv - Neuroscience 2022Quote: ... RNA-seq libraries were generated using the SMARTer Stranded Total RNA-seq kit v2 (Takara Cat no. 634419) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... Media lactate dehydrogenase (LDH) was assayed by enzymatic colorimetric assay (LDH Cytotoxicity Detection Kit, Takara Bio, cat# MK401).
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bioRxiv - Molecular Biology 2022Quote: ... RNA was reverse-transcribed using a PrimeScript RT reagent Kit with gDNA Eraser (Cat: RR047A, TAKARA, Shiga, Japan), and qPCR was performed on a Bio-Rad CFX96 machine (Hercules ...
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bioRxiv - Cancer Biology 2022Quote: ... USA).and converted to cDNA using a Primescript RT reagent kit (Cat# RR037A, DSS Takara Bio India Private Ltd) as per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... The PCR product thus obtained was cloned using the In-Fusion HD Cloning Plus kit (Takara Bio – 638909) into the pMA_F3NeoLoxPPolyFRT vector provided by Dr ...
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bioRxiv - Cancer Biology 2022Quote: ... The PCR products were purified from agarose gels using NucleoSpin Gel and PCR Clean-Up Kit (740609, Clontech) and subcloned into the pENTR/D-TOPO vector (K240020 ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was isolated with Trizol (Thermo) and cDNA was synthesized with Prime Script RT Reagent Kit (Takara). Quantitative PCR was performed with a SYBR Green PCR kit (Toyobo ...
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bioRxiv - Genomics 2023Quote: ... We prepared the adult brain total RNA using the Clontech SmartSeq v4 Ultra-Low Input RNA Kit (Takara). We prepared libraries with the Nextera XT DNA Library Preparation Kit and 96-Sample Index Kit (Illumina ...
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bioRxiv - Bioengineering 2024Quote: ... The cDNA was generated using the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio). The final sequencing library was made using the Nextera XT kit (Illumina) ...
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bioRxiv - Bioengineering 2024Quote: ... The two fragments were assembled in a single In-Fusion reaction (In-Fusion HD Cloning Kit, Takara, 639650), and the PCR-derived regions of the resulting plasmids were confirmed by sequencing.
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bioRxiv - Microbiology 2024Quote: ... The resulting RNA was used as input for the SMARTer smRNA-Seq Kit for Illumina (Takara Cat# 635030). cDNA libraries were prepared according to the manufacturer’s protocol ...
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MALAT1 mediates miR-206/CDC42/PAK1/Paxillin signaling axis to alleviate erectile dysfunction in ratsbioRxiv - Molecular Biology 2024Quote: ... And the obtained RNA was used for reverse transcription using the PrimeScript RT Enzyme Mix I kit (TaKaRa). After the reaction was completed ...
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bioRxiv - Plant Biology 2024Quote: Single or multiple DNA fragments were cloned into binary vector using In-Fusion HD Cloning Kit (Clontech, USA). Briefly ...
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bioRxiv - Plant Biology 2024Quote: ... and inserted into BamHI-digested pGEX-4T-3 by using In-Fusion Smart Assembly Cloning Kit (Takarabio-Clontech). Full-length OcKSL4 cDNA was amplified by RT-PCR using primers 5’-GGATCCATGGCGAATTATCCCATGGAG-3’ (forward ...
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bioRxiv - Plant Biology 2024Quote: ... Reverse transcription reactions were performed using the PrimeScript II 1st strand cDNA Synthesis Kit (TAKARA, Cat. No. 9750), solution was then removed ...
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bioRxiv - Molecular Biology 2024Quote: ... YCplac22 plasmids that carry the cac1-20 mutant allele were constructed using the PrimeSTAR Mutagenesis Basal Kit (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Libraries were prepared using the low-input Clontech SMART-Seq HT with Nxt HT kit (Takara Bio USA) and sequenced using an Illumina NovaSeq 6000 with an S1 100 Cycle Flow Cell (v1.5).
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bioRxiv - Cell Biology 2023Quote: ... The virus titer was quantified using the AAVpro Titration Kit (for Real Time PCR) Ver.2 (Takara Bio) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: cDNA was synthesized from the total RNA of HapT1 cells using the PrimeScript RT Reagent Kit (Takara Bio). The amounts of Pts and Actb were quantified on a LightCycler 480 (Roche ...
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bioRxiv - Cell Biology 2023Quote: The full-length cDNA expression library was constructed using the SMARTer RACE 5’/3’ Kit (Takara Bio, 634858) according to the manufacturer’s instructions for the In-Fusion SMARTer Directional cDNA Library Construction Kit (Takara Bio ...
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bioRxiv - Cell Biology 2023Quote: ... according to the manufacturer’s instructions for the In-Fusion SMARTer Directional cDNA Library Construction Kit (Takara Bio, 634933). 1µg of total RNA extracted from HapT1 with RNeasy Mini Kit (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... linearized GPC sequence was gel purified using NucleoSpin Gel and PCR Clean-up kit (Takara, Cat. No. 740609.5) and then purified using Ampure XP beads (Beckman Coulter ...
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bioRxiv - Molecular Biology 2024Quote: ... Size selected mRNA was then dephosphorylated and library was prepared using SMARTer smRNA-Seq Kit (TAKARA, cat # 635029) according to vendor’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted from drug/vehicle-treated cell lines using NucleoSpin RNA kit (Takara, Catalog no. 740955.50). 2 ug of total RNA was used to synthesize cDNA with qScript Ultra Supermix (Quantabio ...