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Citations for Takara Bio :
251 - 281 of 281 citations for Brucella Blood Agar with Hemin and Vitamin K 15x100mm plate 26ml fill since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: Reverse Transcriptase PCR and Real-Time PCR Cells cultured in either 12- or 24-well plates were washed twice with cold PBS and harvested using a TaKaRa MiniBEST universal RNA extraction kit (Takara, Japan). RNA was purified using the same kit according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... plates with 40 μg/ml X-α-Gal (FUJIFILM Wako Pure Chemical, Osaka, Japan) and 200 ng/ml aureobasidin A (TaKaRa) (QDOGA ...
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bioRxiv - Bioengineering 2024Quote: ... concentrated lentivirus (MOI: 25) was added to non-tissue culture treated 24-well plates that were coated with retronectin (Takara T100B) according to the manufacturer’s instructions and spun at 1,200 × g for 90 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: Mitochondrial network morphology was assessed transfecting hPASMC grown on a 60 mm plate to 50% confluence with 1 μg pDsRed2-mito expression vector (Clontech Laboratories, 632421) and 2 μL Lipofectamine™ 2000 (ThermoFisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... cells were loaded onto a source plate and dispensed in to a SMARTer ICELL8 350 v chip (Takara Bio USA, Cat. # 640019) at 35 nanoliter per well ...
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bioRxiv - Cancer Biology 2020Quote: ... were loaded into each well of a 384-well plate (A1 through D2) and dispensed 50nL per well into a 5,184 well SMARTer™ ICELL8® 3’ DE Chip (Takara Bio) using the ICELL8® Multisample NanoDispenser (MSND ...
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bioRxiv - Biochemistry 2020Quote: A yeast displayed cDNA library that concurrently displays NanoLuc (diversity ∼6×106) was constructed using DNA amplified from the Clontech Mate & Plate Library-Universal Mouse (Normalized) cDNA library (Takara Bio Inc). The yeast library was generated using the previously described lithium acetate yeast transformation method (63) ...
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Plant pathogens convergently evolved to counteract redundant nodes of an NLR immune receptor networkbioRxiv - Plant Biology 2021Quote: ... Single colonies grown on selection plates were inoculated in 5 ml of SD-Leu-Trp overnight at 28 °C (ST0047, Takara Bio, USA). Saturated culture was then used to make serial dilutions of OD600 1 ...
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bioRxiv - Microbiology 2021Quote: ... at 30°C for 3-5 days and assayed for growth on the SD/-Trp/-Leu/-His/-Ade/X-α-gal plates (TaKaRa Bio). Each experiment was repeated at least three times.
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bioRxiv - Neuroscience 2021Quote: ... at P11 were transduced in a 6 well plate using 300 µl of Auto-hLAG3 LV pre-incubated with 30 µl of Lenti-X™ Accelerator (Clontech #631256) for 30 min ...
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bioRxiv - Cell Biology 2022Quote: ... Viral supernatant was harvested 48 h after transfection and added to 6 well plates that had been precoated with 15 μg/ml RetroNectin (Takara Bio Inc.) and blocked with 2% BSA ...
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bioRxiv - Immunology 2020Quote: ... Cells were counted and seeded at 3 million cells in 1 mL of media with 2x hIL-2 into each well of a 6 well plate that was coated with 15 µg/mL of RetroNectin (Takara, Cat# T100A) for 3 hours at room temperature and subsequently washed with 1x PBS ...
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bioRxiv - Genetics 2020Quote: ... Mouse Hsf2bp cDNA was subcloned into the vector pGBKT7 and was used as bait to screen a mouse testis Mate & Plate cDNA library (Clontech Laboratories Inc.). Positive clones were initially identified on double dropout SD (synthetic dropout)/–Leu/–Trp/X-α-Gal/Aureobasidin A plates before further selection on higher stringency quadruple dropout SD/–Ade/–His/–Leu/–Trp/X-α-Gal/Aureobasidin A plates ...
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bioRxiv - Plant Biology 2023Quote: A Y2H library screen was performed as outlined in the Matchmaker Gold “Mate and Plate” Yeast Two-Hybrid System (Takara Bio Inc.) using pGBKT7-CML13 as the bait in yeast strain AH109 ...
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bioRxiv - Immunology 2022Quote: ... The day before transduction, a non-tissue culture treated 24-well plate (Grener Bio one, #662102) was coated with retronectin (Takara Bio, #T100B) in PBS (7μg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... and individual cells were captured in separate wells of a 96-well plate containing 4 μl lysis buffer (1 U/μl RNase inhibitor [Clontech, Cat#: 2313B]), 0.1% Triton (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... and on a SD-Leu-Trp-Ade-His plate containing X-α-gal and supplemented with 0.2 µg/ml Aureobasidin A (Takara Bio, USA). Plates were imaged after incubation for 60–72 hr at 30 °C ...
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bioRxiv - Genomics 2024Quote: ... filtered retroviral supernatants were harvested and applied to HSCs (extracted as described above) in plates coated with Retronectin (20 μg/ml; Takara Bio; #T100B). The transduction process involved spinning at 2,000g for 2 hours at 32°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... concentrated lentivirus was added to the T cells on day 3 at a multiplicity of infection of 10 and subjected to spinoculation in a 24-well plate coated with 30 µg/mL RetroNectin® (Takara, T100B). T cells continued to expand ...
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bioRxiv - Neuroscience 2024Quote: Quantitative PCR was performed in 12 μl of final volume on 96-well plates using the Premix Ex Taq (Takara Biotechnology, #RR037A). Reactions included Segment 1 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were incubated with beads for 24 hours before being transferred to a 24 well plate precoated with retronectin (Takara, Cat#: T100B). Retronectin coating was done by following the manufacturer’s protocol with approximately 10ug/well (5ug/cm^2 ...
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bioRxiv - Immunology 2022Quote: ... 1 mL of retroviral supernatant was added to a non-tissue culture-treated 24-well plate pre-coated with Retronectin (FN CH-296; Takara Shuzo, Otsu, Japan) and centrifuged for 90 min at 2000×g ...
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bioRxiv - Neuroscience 2022Quote: ... single GFP+ cells were captured into 96-well plate with 11.5 μl lysis buffer and RNase Inhibitor without the CDS IIA Oligo (Takara Bio, Mountain View, CA), and stored at −80°C until used for construction of cDNA libraries ...
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bioRxiv - Neuroscience 2023Quote: ... The qPCR reaction was performed on 96-well plates in a final volume of 12 μL using the Premix Ex Taq Probe based qPCR assay (Takara Biotechnology, ref. RR390A). All reactions were run in duplicate on a StepOnePlus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Immunology 2023Quote: ... M-IgA was expressed by transfecting a pair of alpha heavy and kappa light chain genes into FreeStyle CHO-S cells that were grown in a 24-well plate according to the manufacturer’s protocol (CHOgro High Yield Expression System, Takara Bio, Shiga, Japan). For large-scale antibody production ...
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bioRxiv - Immunology 2024Quote: ... 0.5mL T cells at 2 × 106 cells mL-1 were mixed with 1.5mL lentiviral supernatant and centrifuged in 24-well plates pre-coated with 7 µg mL-1 Retronectin (Takara Bio, San Jose, USA) at 1000 x g for 20min ...
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bioRxiv - Immunology 2024Quote: ... 2.5 ml of retroviral supernatant was transferred to a non-treated 24-well plate pre-coated with RetroNectin reagent (Takara cat# T100A/B) and centrifuged at 2000 g at 4°C for 2 hours to transduce T cells ...
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bioRxiv - Evolutionary Biology 2022Quote: A yeast two-hybrid prey cDNA library of Trichoplax whole animal RNA was prepared in the pGADT7- AD plasmid vector using the Make Your Own “Mate & Plate™” Library System (Takara Bio, California USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... tabaci adults was used for the synthesis of first strand cDNA utilizing the make your own mate & plate library system with CDS III adaptor primer and smart III oligo (template switching) as per manufacturer’s instructions (Takara Bio, San Jose, CA, USA). The first strand cDNA was used as template for long distance PCR with 5’ and 3’ primers ...
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bioRxiv - Cancer Biology 2023Quote: Retroviral supernatant was collected two and three days after transfection of GP2-293T cells and concentrated onto wells of a 6 well plates coated with Retronectin (Takara Bio, 5 ug/ml) by spinning at 2500g for 90 minutes at 32° C ...
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bioRxiv - Neuroscience 2024Quote: ... Purified DNA was transfected into nearly confluent (80–90%)10 cm2 plates of low passage LentiX 293T cells (#632180, Takara Bio, San Jose, CA, USA), using a calcium phosphate transfection kit (Takara #631312) ...