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Citations for Takara Bio :
1 - 50 of 281 citations for Brucella Blood Agar with Hemin and Vitamin K 15x100mm plate 26ml fill since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... RT-PCR products of stage 2 ray florets using c25599_g2_i1 Fill-F and c25599_g2_i1 Fill-R primers (Table S2) were digested with SacI (Takara Bio Inc). Reaction mixture was consisted of 2.5 µL of PCR product ...
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bioRxiv - Cell Biology 2022Quote: ... on agar plates containing indicated concentration of AureobasidinA (Clontech), Calcofluor White (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2020Quote: ... The transformants were grown on SD -Trp plates (Clontech, 2% agar) for selection ...
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bioRxiv - Systems Biology 2021Quote: ... selecting on SD -HIS agar plates (Takara Bio, 630411; Diagonal GmbH&CoKG, Y1751). The correct genomic context insertion of GFP of single colonies was confirmed by PCR using a combination of primers which also allowed the confirmation of the intron deletion strain ...
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bioRxiv - Bioengineering 2020Quote: ... Transformed strains were grown at 30 °C on agar plates containing minimal SD base (Clontech, cat. 630411) and –His/–Trp/–Ura dropout supplement (Clontech ...
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bioRxiv - Microbiology 2024Quote: ... yeast (OD 0.8) were serial diluted at 10-fold on SC minimal dropout (-Trp, -Ura, -Leu) agar plates (Clontech) at 30 LJ.
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bioRxiv - Biochemistry 2021Quote: ... and Proteinase K (Takara). Polymerase chain reaction (PCR ...
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bioRxiv - Microbiology 2022Quote: ... The plasmids were extracted from all the colonies which grew on QDO/X/A agar plate using Easy Yeast Plasmid Isolation Kit (TaKaRa). PCR was carried out using the extracted plasmids as template ...
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bioRxiv - Genetics 2024Quote: ... then digested with Proteinase K (Takara) at 55lJ overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... and proteinase K (9034, Takara Bio). The reactions were terminated by addition of 15% trichloroacetic acid ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by incubation with proteinase K (Takara) for 2 h at 55 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and 250 μg/mL proteinase K (Takara, Dalian), incubated at 55 °C for 1h ...
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bioRxiv - Microbiology 2023Quote: ... PK-15 cells were transfected with PX459-Ifnar1-k/o or PX459-Stat2-k/o plasmids using the TransIT-X2 Dynamic Delivery System (TaKaRa, Cat# V6100) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 12.5 mM HEPES-KOH (pH7.4) and 12.5% Proteinase K (TaKaRa)] ...
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bioRxiv - Molecular Biology 2022Quote: ... Five microliters of DNase I (15 K units, TaKaRa Bio), 0.2 U/μL of ribonuclease inhibitor (porcine liver ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.5% SDS) and 50 μl of proteinase K (Takara, 9034) were added ...
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bioRxiv - Neuroscience 2023Quote: ... pH 8.5) containing proteinase K (Takara Bio Inc., Otsu, Japan). This mixture was incubated at 55 °C for at least 12 hours ...
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bioRxiv - Evolutionary Biology 2023Quote: ... After 1 μg/ml Proteinase K (Takara Bio, Shiga, Japan) treatment ...
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bioRxiv - Developmental Biology 2021Quote: ... the RNA-protein mix was catalyzed by proteinase K (Takara, 9034) at 55 °C for 1 h ...
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bioRxiv - Genetics 2022Quote: ... and Nucleospin Blood Midi kit (Takara Cat. #740954) (for CRISPR/Cas12a screening) ...
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bioRxiv - Microbiology 2022Quote: ... The purified fiber was treated by adding proteinase K (PK) (Takara ST 0341) to 20 µL of sample (200 ng as estimated by dsDNA Qubit fluorometric quantification ...
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bioRxiv - Genetics 2024Quote: Total RNA was extracted from 250 µl of EDTA-anticoagulated venous blood samples collected on-site using RNAiso Blood solution (Cat# 9112; Takara Bio, Japan) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... Interactions were evaluated on agar that was -His or -His -Ade (Clontech, now Takara Bio). Growth in the absence of either supplement indicates transcription from reconstituted GAL4 proteins ...
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bioRxiv - Developmental Biology 2023Quote: ... Interactions were evaluated on agar that was -His or -His -Ade (Clontech, now Takara Bio). Growth in the absence of either supplement indicates transcription from reconstituted GAL4 proteins ...
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bioRxiv - Bioengineering 2022Quote: ... Blood samples were first lysed by Trizol reagent (TAKARA). Then ...
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bioRxiv - Genetics 2022Quote: ... genomic DNA was extracted using NucleoSpin Blood XL (Clontech) and the shRNA sequence was amplified as previously described50 ...
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bioRxiv - Biochemistry 2020Quote: ... Transformed cells were plated on a selection agar (6.9g/L yeast nitrogen base without amino acids, 0.62g/L Clontech -Leu/-Trp/-Ura dropout supplement ...
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bioRxiv - Plant Biology 2024Quote: ... Transformed yeast cells were grown under selective conditions in a minimal medium (46.7 g L-1 Minimal SD Agar Base [TaKaRa], 0.78 g L-1 uracil dropout supplement [TaKaRa]). Cytokinin transport assays were performed according to the method described by Zhao et al ...
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bioRxiv - Molecular Biology 2022Quote: ... 1.8 μl reaction stop solution containing 5.3% SDS and 6.6 mg/mL proteinase K (9034, Takara) was added and the sample was incubated for 60 min at 37°C ...
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bioRxiv - Genetics 2022Quote: ... gDNA was extracted using a Nucleospin Blood Maxi kit (Takara, Cat. #740950) (for CRISPR/Cas9 screening ...
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bioRxiv - Plant Biology 2024Quote: ... Transformed yeast cells were grown under selective conditions in a minimal medium (46.7 g L-1 Minimal SD Agar Base [TaKaRa] ...
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bioRxiv - Cell Biology 2022Quote: ... Genomic DNA was purified using the Nucleospin Blood XL kit (Takara Bio, #740950.10) and amplified by index PCR with barcoded primers ...
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bioRxiv - Cancer Biology 2023Quote: ... Genomic DNA was extracted using a NucleoSpin Blood XL kit (740950.50; Takara Bio) and further cleaned up by a OneStep PCR Inhibitor Removal Kit (D6030 ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA was extracted with Machery-Nagel NucleoSpin Blood XL (Takara, Cat#740950). sgRNAs were PCR amplified from gDNA (Clontech ...
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bioRxiv - Plant Biology 2023Quote: ... Total RNA was isolated from 14-day-old thalli of the wild type and Mpacl5 mutants grown on the half-strength B5 agar medium using NucleoSpin RNA Plant (Takara). The sequence libraries were generated by TruSeq RNA Sample Prep Kit (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: ... non-adherent plate (Takara Bio). Cells were grown for 14 days ...
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bioRxiv - Molecular Biology 2022Quote: ... bound RNAs were eluted via 30min incubation at 55°C in wash buffer supplemented with 0.5 μg/μL Proteinase K and 0.1% SDS and isolated using Qiazol/chloroform separation and NucleoSpin RNA columns (Takara). Luciferase control RNA is spiked in to each sample (5ng/sample ...
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bioRxiv - Molecular Biology 2021Quote: Slide embedded tissue sections were incubated in PBS containing 0.2 mg/ml saponin (Nacalai teques) and 10 μg/ml Proteinase K (Takara Bio) for 20 min at RT ...
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bioRxiv - Immunology 2021Quote: ... Following bulk BCR and TCR are prepared using SMARTer Mouse BCR IgG H/K/L Profiling Kit and SMARTer Mouse TCR a/b profiling kit separately (Takara). Based on the extracted mRNA amount of each sample ...
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bioRxiv - Cell Biology 2021Quote: ... The sequence encoding mCherry in frame with the polybasic sequence and CAAX motif of human K-Ras (GKKKKKKSKTKCVIM) for targeting to the plasma membrane was generated by amplification of mCherry using the pmCherry-N1 (Clontech) plasmid as template and the following primers ...
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bioRxiv - Biophysics 2021Quote: ... AY457063.1) and PIKfyve-KYA hyperactive mutant (E1620>K, N1630>Y, S2068>A) were cloned into PCMV-HA-N vector (Clontech) and gifted by Lois Weisman lab ...
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bioRxiv - Biochemistry 2023Quote: TssM193-490 coding region was obtained by gene synthesis (IDT) and cloned into pOPIN-K vector45 using the In-Fusion HD Cloning Kit (Takara Clontech). Point mutations were introduced using the QuikChange Lightning kit (Agilent Technologies).
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was extracted and purified using a Nucleospin Blood XL kit (Takara Bio, #740950.10). The guides were amplified and barcoded by PCR using NEB Next Ultra ii Q5 MM (M0544L) ...
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bioRxiv - Plant Biology 2024Quote: ... The dilutions were plated on selective -LT medium as a growth control and on -LTH medium (7.6 g/L Yeast Nitrogen Base [YNB], 20 g/L glucose, 0.62 g/L DO supplement -leu -trp -his [Clontech], 15 g/L agar) for the protein interaction assay ...
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bioRxiv - Systems Biology 2020Quote: ... proteins in whole cell extract and immunoprecipitated fractions were digested with 2 mg/mL proteinase K (Takara Bio Inc., Kusatsu, Japan) at 42°C for 2 h ...
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bioRxiv - Bioengineering 2022Quote: ... Purified insert DNA was cloned into the linearized modRNA plasmid (5MCS3)(K et al., 2018) using the In-Fusion Cloning Kit (Takara Bio). The DNA template for modRNA synthesis was PCR amplified from the successfully cloned 5MCS3 plasmid followed by PCR purification using DNA Clean & Concentrator-5 (Zymo Research) ...
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bioRxiv - Cell Biology 2020Quote: ... was added to the StrePD beads and incubated at 37 °C for 10 minutes followed by an incubation with Proteinase K (Takara, #9034) at 60 °C overnight ...
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bioRxiv - Biochemistry 2023Quote: TssM193-490 coding region was obtained by gene synthesis (IDT) and cloned into pOPIN-K vector45 using the In-Fusion HD Cloning Kit (Takara Clontech). Point mutations were introduced using the QuikChange Lightning kit (Agilent Technologies).
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bioRxiv - Cell Biology 2022Quote: Genomic DNA was extracted from HEK 293T cells using a NucleoSpin Blood kit (Takara Bio; 740951) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... banked cell pellets were rapidly thawed and processed using the Nucleospin® Blood XL kit (Takara) following the manufacturer’s instructions ...