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Citations for Takara Bio :
2701 - 2750 of 4905 citations for Thyroxine ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... the total RNA was reverse transcribed to cDNA using the Prime ScriptTM RT reagent kit (TaKaRa Biotechnology). qPCR was performed using the SYBR Premix Ex Taq II Kit (TaKaRa Biotechnology ...
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bioRxiv - Molecular Biology 2022Quote: ... The DNA band was cut out and purified using the Nucleospin® Extract II Kit (Clontech Laboratories) protocol ...
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bioRxiv - Plant Biology 2022Quote: ... The downstream genomic region was then amplified using the primer set MpHEC1_CDS_IF_F and MpHEC1_3UTR_IF_R2 and integrated into the first binary plasmid with an In-Fusion HD Cloning Kit (Clontech) using the BamHI and AscI sites ...
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bioRxiv - Microbiology 2022Quote: ... DNA fragments were assembled into pEMC vector one-by-one using in-fusion HD cloning kit (Takara), generating pEMC-eGFP-MBaMV ...
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bioRxiv - Microbiology 2022Quote: ... and used for plasmid DNA preps using the Endotoxin-Free Nucleobond Plasmid Midiprep kit (Takara Bio; 740422.10). The HIVDEP library was sequenced and contains all 4,191 sgRNAs included in the synthesis (GEO Dataset ...
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bioRxiv - Molecular Biology 2022Quote: ... rAAVs were purified using AAVpro® Purification Kit for All Serotypes (Cat # 6666, Takara Bio USA, CA) and each of the purified rAAVs were tested in separate transwell BBB cultures with hCMEC/D3 cells and primary astrocytes at MOI of 1e4 ...
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bioRxiv - Molecular Biology 2022Quote: cDNA was synthesized with 1 μg of total RNA using the Prime ScriptTM RT Reagent Kit (Takara) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The SMARTSeq HT Ultra Low Input Kit was used for full-length cDNA synthesis and amplification (Clontech), and Illumina Nextera XT library was used for sequencing library preparation ...
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bioRxiv - Bioengineering 2024Quote: ... Viral genome number was quantified by real-time PCR with AAVpro Titration Kit Ver.2 (Takara, 6233) according to manufacturer’s instruction ...
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bioRxiv - Genomics 2024Quote: ... Both reagents were included in SMART-Seq Single Cell PLUS kit (Takara Bio, San Jose, CA, U.S.). Immediately after sampling in lysis buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Sequencing libraries were prepared using the SMARTer v4 Ultra Low Input RNA Kit (Clontech, Mountain View, CA). Sequencing was performed on NovaSeq S4 150PE platform (Illumina ...
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Regulation of Diseases-Associated Microglia in the Optic Nerve by Lipoxin B4 and Ocular HypertensionbioRxiv - Molecular Biology 2024Quote: ... mRNA was converted to cDNA using SMARTer v4 Ultra Low Input RNA Kit (Clontech, Mountain View, CA). A Diagenode Bioruptor Pico was used to fragment the cDNA ...
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bioRxiv - Molecular Biology 2024Quote: ... and ligated into the digested lentiCRISPRv2 backbone using the DNA ligation kit (Mighty Mix) (Takara, Cat# 6023). The ligated DNA was then transformed into One Shot Stabl3 competent cells (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... The viral titer was measured using a Lenti-X qRT‒PCR Titration Kit (#631235, TaKaRa, Shiga, Japan).
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bioRxiv - Neuroscience 2024Quote: ... The target genes were amplified by qPCR according to the instructions of kits (Cat.no. 638315 Takara, Japan), reversely ...
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bioRxiv - Neuroscience 2024Quote: ... followed by a T7 endonuclease I (T7EI) assay using the Guide-it Mutation Detection Kit (Takara Bio) following the standard protocol for mismatch detection.
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bioRxiv - Cell Biology 2023Quote: ... DUSP13 deficient in phosphatase activity (=Dusp13_Mut) was generated using the PrimeSTAR® Mutagenesis Basal Kit (Takara Bio). The Dusp family contains the amino acid cysteine ...
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bioRxiv - Genomics 2024Quote: ... Library construction was performed based on the manufacturer’s recommendation for SmarterStranded V2 kit (Takara Bio, California, USA). The final library quantity was measured by KAPA SYBR FAST qPCR and library quality was evaluated by TapeStation D1000 ScreenTape (Agilent Technologies ...
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bioRxiv - Immunology 2024Quote: ... cDNA libraries were prepared using SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (Takara, Japan) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... except that an alternative oligo-dT primer from the SMARTer PCR cDNA Synthesis kit (Clontech Laboratories, Inc.), which also included a random 10mer sequence as a unique molecule identifier (UMI ...
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bioRxiv - Cell Biology 2024Quote: ... and the YFP cDNA was assembled using an In-fusion HD cloning kit (Takara-Bio, Shiga, Japan). (2 ...
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bioRxiv - Microbiology 2024Quote: ... The reverse transcription was performed using the PrimeScript RT reagent kit (Takara, Saint-Germain-en-Laye, France) with a primer oligo-d(T)-AP (5’ GACCACGCGTATCGATGTCGACTTTTTTTTTTTTTTTTv 3’) ...
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bioRxiv - Immunology 2023Quote: ... Illumina libraries were constructed using SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (TaKaRa, Japan). RNA-seq libraries were sequenced at read lengths 2 × 151bp on the Illumina Novaseq 6000 (Illumina) ...
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bioRxiv - Molecular Biology 2024Quote: ... or 5 ng of total RNA (with a SMARTer Stranded Total RNA-Seq Kit v3; Takara Bio) was used.
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bioRxiv - Microbiology 2024Quote: ... Each cDNA was generated using a Clontech SMART-Seq HT Kit (Takara Clontech, Mountain View, CA, USA), and each library was prepared using a Nextera XT DNA Library Prep Kit (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... Each cDNA was generated using a Clontech SMART-Seq HT Kit (Takara Clontech, Mountain View, CA, USA), and each library was prepared using a Nextera XT DNA Library Prep Kit (Illumina ...
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bioRxiv - Genetics 2023Quote: ... The PCR produc t was purified with a PCR purification kit (Takara Biomedical Technology, Bei jing, China) and was cloned into a pMDTM18-T vector (Takara Biomedical Tec hnology ...
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bioRxiv - Developmental Biology 2023Quote: ... and RNA-seq libraries were generated using the SMARTer ThruPLEX DNA-Seq Kit (Takara Bio, Cat. # R400674). Each library was indexed using the DNA Unique Dual Index Kit (Takara Bio ...
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bioRxiv - Cell Biology 2023Quote: All viruses and lentiviruses were produced by transfecting Hek293T cells using CalPhos Mammalian Transfection Kit (Takara Bio) according to the manual in DMEM ...
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bioRxiv - Molecular Biology 2023Quote: ... Then the cDNA was synthesized using PrimeScript RT Reagent Kit with gDNA Eraser (Perfect Real Time) (TaKaRa). Real-time quantitative PCR was performed as previously described [13 ...
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bioRxiv - Microbiology 2023Quote: ... All cloning experiments were performed using an In-Fusion HD Cloning Kit (Takara Bio Inc., Shiga, Japan), and plasmid sequences were confirmed using a DNA sequencing service (Core Instrumentation Facility ...
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bioRxiv - Immunology 2023Quote: ... followed by RNA reverse transcription by SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech). After enzymatic fragmentation of cDNA samples by KAPA Frag kit (KAPA Biosystems) ...
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bioRxiv - Immunology 2023Quote: ... RNA was extracted and libraries prepared using SMARTer Stranded RNA-Seq Kits according the manufacturer’s protocols (Takara) by the Integrated Genomics Operation (IGO ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was prepared using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (634898, Takara Bio.). The cDNA quality was examined on Agilent TapeStation system (RRID:SCR_019547 ...
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bioRxiv - Cancer Biology 2023Quote: ... bacteria cells were pelleted and BAC DNA was extracted using the NucleoBond Xtra BAC kit (Takara, 740436) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and inserted into either the pcDNA3.1+ or pcDNA3.1_N-eGFP vectors using the In-Fusion HD Cloning Kit (Takara). Following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Quantitative PCR was performed using the Mir-X miRNA qRT-PCR TB Green Kit (Takara Bio, USA) using the protocol provided by the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... FD0564)-digested pCRIS-PITChv2-FBL plasmid (addgene #63672) using the In-Fusion HD cloning kit (Takara Bio, 639650). To create the sgRNA-plasmid backbone ...
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bioRxiv - Cell Biology 2023Quote: ... by replacing EGFP-coding sequences with mCherry-coding sequences using In-Fusion® HD Cloning Kit (Clontech).
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bioRxiv - Cell Biology 2023Quote: ... The real-time PCR reaction was performed using One Step SYBR Prime Script RT-PCR Kit (TakaRa). The primers for 14-3-3β ...
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bioRxiv - Microbiology 2023Quote: ... The RNA was then reverse-transcribed into complementary DNA (cDNA) using the PrimeScript cDNA Synthesis Kit (Takara). Synthesized cDNA was utilized as a template for polymerase chain reaction (PCR ...
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bioRxiv - Plant Biology 2023Quote: ... Two biological replicates were processed for next-generation library preparation using the ThruPLEX DNA-seq kit (Takara). Next ...
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bioRxiv - Microbiology 2023Quote: ... The RNA was then reverse-transcribed into complementary DNA (cDNA) using the PrimeScript cDNA Synthesis Kit (Takara). The DENV 3 cDNA was subsequently amplified by PCR ...
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bioRxiv - Immunology 2023Quote: ... 500 ng of RNA was used for cDNA synthesis using a PrimeScript RT Reagent Kit (Takara, RR037A). 2 μL of diluted cDNA (1:50 ...
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bioRxiv - Microbiology 2023Quote: Total RNA of each strain was extracted with the RNA extraction kit (Takara biomedical technology, Beijing, China) following the manufacturer’s instruction ...
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bioRxiv - Genomics 2023Quote: ... The obtained PCR products were purified using NucleoSpin Gel and PCR Cleanup kit (Takara Bio, Shiga, Japan) and sequenced directly or were cloned into the pTAC-1 vector (Biodynamics ...
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bioRxiv - Immunology 2023Quote: ... RNA extracted and amplified (10 ng RNA/sample) using SMART-Seq® HT kit (Takara Bio, #634455) according to the manufacture’s instruction ...
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bioRxiv - Immunology 2023Quote: ... RNA extracted and amplified (10 ng RNA/sample) using SMART-Seq® HT kit (Takara Bio, #634455) according to the manufacture’s instruction ...
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bioRxiv - Immunology 2023Quote: ... RNA was extracted and libraries prepared using SMARTer Stranded RNA-Seq Kits according the manufacturer’s protocols (Takara) by the Integrated Genomics Operation (IGO ...
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bioRxiv - Immunology 2023Quote: ... cDNA libraries were constructed using the SMARTer Stranded Total RNA-Seq Kit (v2) – Pico Input Mammalian (Clontech) following the manufacturer’s instructions ...