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Citations for Takara Bio :
2501 - 2550 of 4905 citations for Thyroxine ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... RNA was isolated from cells using the NucleoSpin RNA Plus kit of Clontech Laboratories (Takara Bio Company). cDNA was constructed using the In-Fusion SMARTer Directional cDNA Library Construction kit of Takara Bio ...
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bioRxiv - Cancer Biology 2021Quote: ... QPCR reactions were performed according to the manufacturer’s instructions using SYBR® Premix Ex Taq kit (TaKaRa). All reactions were performed in triplicates ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was extracted from cells and quantified using RNA Isolation Reagent kit (9109, Takara, Tokyo, Japan). cDNA was obtained from 1 µg of total RNA from each sample (Super M-MLV reverse transcriptase ...
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bioRxiv - Cell Biology 2019Quote: ... RNA was extracted and cDNA was prepared using the SMARTer v3.0 or v4.0 cDNA synthesis kit (Clontech). RNA-Seq was performed on an Illumina Nextseq 500 machine ...
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bioRxiv - Developmental Biology 2021Quote: ... Library preparations were done using SMARTer® Stranded RNA-Seq Kit (Clontech Laboratories, Inc. #634836 or #634837) according to manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2020Quote: ... and processed for library generation using SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (TaKaRa) following the manufacturer’s recommendations ...
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bioRxiv - Immunology 2021Quote: ... and all resulting RNA was used as input for the SMART-seq v4 cDNA synthesis kit (Takara) with 12 cycles of PCR amplification ...
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bioRxiv - Cell Biology 2020Quote: ... and cDNA was synthesized using the SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio Europe). Sequencing libraries were prepared with the Nextera® XT DNA Library Preparation Kit for Illumina® ...
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bioRxiv - Genetics 2021Quote: ... Total RNA was reverse transcribed to cDNA using the PrimeScript cDNA synthesis kit (Takara Bio, Kusatsu, Japan). Quantitative PCR (qPCR ...
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bioRxiv - Immunology 2021Quote: ... polyA-selected transcriptome libraries were constructed using the SMART-seq v4 Ultra Low Input RNA Kit (Takara) combined to the Nextera XT DNA Library Preparation Kit (Illumina ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 ng of total RNA was used with the SMARTer Stranded Total RNA-Seq Kit v2 (Takara) in accordance with the low-throughput protocol ...
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bioRxiv - Immunology 2021Quote: ... Reverse transcription was performed with a PrimeScript RT Reagent Kit with gDNA Eraser (Takara, Cat no. RR047A) and qRT-PCR was performed on a StepOnePlus Real-time PCR system (Applied Biosystems ...
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bioRxiv - Evolutionary Biology 2021Quote: ... we ligated an RNA oligo of the SMART RACE cDNA Amplification Kit (Takara Bio Inc., Shiga, Japan) at 3-end of the total RNA during reverse transcription ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA-seq libraries were performed with SMARTer® Stranded Total RNA-Seq Kit v2 (Takara - rRNA depletion) and sequenced in 100bp PE reads run on NovaSeq 6000 (Illumina) ...
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bioRxiv - Microbiology 2022Quote: ... cDNA libraries were prepared using the SMARTer® Universal Low Input RNA Kit for Sequencing (Takara Bio), without rRNA depletion ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA synthesized from 1 μg total RNA using PrimeScript™ RT reagent Kit with gDNA Eraser (Takara). Real-time PCR was performed using TB Green® Premix Ex Taq™ II (Tli RNaseH Plus ...
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bioRxiv - Neuroscience 2021Quote: The sgRNA sequences were amplified using Guide-it™ CRISPR Genome-Wide Library PCR Kit (Takara, 632651) and subjected to the high-throughput amplicon sequencing on NextSeq500 ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA ligation was done using standard ligation techniques (Takara DNA Ligation kit ver.2.1, Takara, Otsu, Japan). For the transformation ...
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bioRxiv - Molecular Biology 2020Quote: ... The 3xFlag sequence was then ligated into the digested eGFP-Usp9x plasmid (Takara DNA ligation kit #6023).
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bioRxiv - Molecular Biology 2020Quote: The RNA-seq library in oocytes was prepared using the SMART-Seq® Stranded Kit (TaKaRa, 634442). Total RNAs obtained using NucleoSpin® RNA Plus XS (TaKaRa ...
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bioRxiv - Cell Biology 2022Quote: ... and complementary DNA of miR was synthesized using the Mir-X miRNA First-Strand Synthesis Kit (TaKaRa). RT-qPCR was performed with TB Green Premix Ex Taq II (Tli RNaseH Plus ...
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bioRxiv - Genomics 2020Quote: ... for subsequently cloned using the InFusion HD Cloning Kit protocol (Takara Bio, Saint-Germain-en-Laye, France). All plasmids were confirmed by Sanger sequencing ...
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bioRxiv - Genomics 2020Quote: ... The titer of each virus was determined using the Lenti-X Go-Stix Plus kit (Takara Bio), according to the manufacturer’s instruction.
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bioRxiv - Immunology 2021Quote: ... using One Step TB Green(tm) PrimeScript(tm) RT-PCR Kit II (SYBR Green) (RR086B, TaKaRa, JAPAN). Relative copy number was determined by calculating the fold-change difference in the gene of interest relative to GAPTH ...
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bioRxiv - Molecular Biology 2019Quote: ... apis mycelia was reverse transcribed to cDNA using the RevertAid First Strand cDNA Synthesis Kit (TaKaRa, China) and Oligo dT primers ...
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bioRxiv - Immunology 2019Quote: ... and cDNA was prepared using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara). Library construction was performed using the NexteraXT DNA sample preparation kit (Illumina ...
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bioRxiv - Developmental Biology 2019Quote: ... Library preparation followed the SMARTERÒ stranded Total RNA-Seq Kit v2 – Pico Input Mammalian (Takara Bio, Japan). Due to the degraded nature of input material we omitted the fragmentation step ...
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bioRxiv - Genetics 2019Quote: BAC clone (BAC PAC RPCI- 98 library) DNA was extracted using a MIDI-prep kit (Clontech #740410). The probe for the bw locus was Clone BACR48M01 ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA-Seq libraries were prepared using the SMART-Seq v4 Ultra Low Input RNA Kit (Takara, 634889) from total RNA ...
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bioRxiv - Cancer Biology 2019Quote: ... And then total RNAs were reversely transcribed into cDNA using MLV RTase cDNA Synthesis Kit (Takara, Japan). Quantitative RT-PCR was conducted in Roche Applied Science LightCycler 480II Real-time PCR systems (Roche Applied Science ...
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Mutant ACTB mRNA 3′UTR Promotes Hepatocellular Carcinoma Development by Regulating miR-1 and miR-29abioRxiv - Cancer Biology 2019Quote: ... the isolated RNA was reverse transcribed to cDNA using a PrimeScript RT reagent Kit (Takara, Dalian China). Real-time PCR analyses were performed with SYBR Green (Takara ...
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bioRxiv - Developmental Biology 2019Quote: ... and then cDNA was synthesized using the PrimeScript reverse transcription (RT) reagent kit (6210B; Takara Bio. Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... 1 ng of total RNA was amplified using the SMARter Ultra Low amplification kit (Clontech, Mountain, CA) with PCR amplification to generate sufficient cDNA for library construction ...
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bioRxiv - Microbiology 2019Quote: ... the cDNA was purified and eluted in 20ul of elution buffer (NucleoSpin PCR Clean-up Kit, Clontech). The immunoglobulin PCRs were set up with Platinum Taq High-Fidelity DNA Polymerase (Life Technologies ...
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bioRxiv - Developmental Biology 2019Quote: ... The PCR products were extracted from agarose gel using MiniBEST Agarose Gel DNA Extraction Kit (Takara, Japan) based on manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... and cDNA synthesized from mRNA using SMART-Seq® v4 Ultra™ Low Input RNA Kit (Clontech). Library was then constructed and sequenced on Illumina HiSeq platform with the PE150 strategy ...
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bioRxiv - Molecular Biology 2020Quote: ... Then cDNA was synthesized according to the protocol of the RT-PCR kit (Takara; Kusatsu, Shiga, Japan) and used as templates for quantitative PCR ...
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bioRxiv - Plant Biology 2020Quote: ... and first-strand cDNA was synthesized using the PrimeScript II 1st strand cDNA Synthesis Kit (TAKARA Bio), according to the manufacturer’s protocol.
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bioRxiv - Genetics 2021Quote: ... and One Step TB Green™ PrimeScript™ RT-PCR Kit II (SYBR Green) (TaKaRa RR086B, Japan).
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bioRxiv - Neuroscience 2021Quote: ... total RNA was used for reverse transcription (RT) using the SMARTScribe Reverse Transcription Kit (TaKaRa, Cat #639537).
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bioRxiv - Evolutionary Biology 2021Quote: ... pre-amplified (DFRS empty plasmid primers) and pre-digested by the same enzymes (In Fusion Kit; Clontech). The miR-137 or miR-122 complementary sequences were thereby located on the 3’UTR of the RFP gene.
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bioRxiv - Neuroscience 2020Quote: ... The lentiviral genome copy number was calculated using the Clontech Lenti-X qRT-PCR Titration kit (Takara).
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bioRxiv - Developmental Biology 2020Quote: ... RNA-seq libraries were prepared with the SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio) with poly(A ...
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bioRxiv - Biochemistry 2020Quote: ... and cell toxicity was evaluated using the LDH Cytotoxicity Detection Kit (Cat #MK401; Takara Bio, Shiga, Japan).
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bioRxiv - Neuroscience 2020Quote: Cell line CUT&RUN libraries were prepared using the SMARTer ThruPLEX DNA-seq Kit (Takara Bio R400676), with the following PCR conditions ...
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bioRxiv - Cancer Biology 2019Quote: ... and subsequently subjected to reverse transcription using PrimeScript™ RT reagent Kit (TaKara Bio, Mountain View, CA). Quantitative real-time PCR was performed with Premix Ex Taq (TaKara Bio ...
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bioRxiv - Cell Biology 2021Quote: DNA insertion and site-directed mutagenesis were performed using an In-Fusion HD Cloning Kit (TaKaRa Bio) or by restriction enzyme fragmentation ...
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bioRxiv - Plant Biology 2021Quote: ... All cloning was done using In Fusion® HD Cloning kit (Takara, California, USA; Cat. No. 639649). First ...
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bioRxiv - Plant Biology 2020Quote: ... and reverse-transcribed with a random hexamer using the PrimeScript II 1st strand cDNA Synthesis kit (TaKaRa). Gene specific primers were used to amplify cDNA by PCR using Ex Taq Hot Start Version (Takara) ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentrations of cell lysates were measured using a bicinchoninic acid protein assay kit (Takara Bio Inc.). Cell lysate containing the same amount of protein was mixed with 10 volumes of methanol and then centrifuged at 20,000 × g for 30 min to precipitate proteins ...