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Citations for Takara Bio :
201 - 250 of 847 citations for Recombinant Human Ribonucleotide Reductase M2 B TP53 inducible His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... 0.8 U/μl recombinant RNase inhibitor (Takara-Bio)] and incubated at room temperature for 10 min ...
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bioRxiv - Evolutionary Biology 2023Quote: ... recombinant protein (vector pColdTM TF-DNA, Takara Clontech) to use as the antigen ...
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bioRxiv - Evolutionary Biology 2023Quote: ... recombinant protein (vector pColdTM TF-DNA, Takara Clontech) to use as the antigen ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1.2 U recombinant inhibitor (Cat. 2313B, TaKaRa) or SEQURNA Thermostable RNase inhibitor (Cat ...
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bioRxiv - Molecular Biology 2024Quote: ... and 4 U recombinant inhibitor (Cat. 2313B, TaKaRa) or SEQURNA thermostable RNase inhibitor (Cat ...
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bioRxiv - Genetics 2022Quote: ... then washed in water and re-suspended in 125-250mL -leu - his galactose liquid culture (Takara Bio Minimal SD Bases, Takara Bio DO Supplement -His/-Leu). Cultures were grown to saturation ...
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bioRxiv - Immunology 2019Quote: ... conjugated to vinculin or the vinculin A50I mutant were cloned into the doxycycline-inducible Tet-On 3G plasmid system (Takara Bio). HoxB8-conditional progenitors (expressing Cas9 and control sgRNA or Vcl (2 ...
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bioRxiv - Cell Biology 2021Quote: γ2 mCherry and tethered GluA2 (flop isoform)::γ260 were subcloned into the doxycycline-inducible expression vector pBI-Tet (Clontech, #6152-1) using the restriction sites MluI/XbaI and MluI/NheI ...
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bioRxiv - Biochemistry 2022Quote: A HeLa-T-PFK1-mEGFP stable cell line (hereafter, HeLa-T-PFK1G) was generated using the HeLa Tet-On® 3G inducible expression system (Clontech) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... we first produced lentivirus with Pup (E) plasmid Bio-Pup (E)-IRES-BFP within the Tet-On 3G inducible expression system (Clontech 631168), and infected OVCAR-5 cells for 48 hours ...
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bioRxiv - Cancer Biology 2019Quote: ... Lentivirus was then generated and infected to establish Tet-inducible cells lines cultured in 10% Tet-system approved FBS (Clontech #631106) and 500 μg/mL G418 (Life Technologies #10131035) ...
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bioRxiv - Cell Biology 2021Quote: ... we first produced lentivirus with Pup(E) plasmid Biotin-Pup(E)-IRES-BFP within the Tet-On 3G inducible expression system (Clontech 631168), and infected Jurkat cells for 48 hours ...
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bioRxiv - Cell Biology 2022Quote: ... pLVX TRE 3G and pLVX EF1Į Tet3G were part of Tet-On® 3G Inducible Expression Systems (631363, Takara Bio Inc.). pCMV6-Entry Myc DDK CD147 (BSG ...
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bioRxiv - Cancer Biology 2023Quote: ... MYCN coding region was PCR amplified from HA-MYCN construct and cloned into the doxycycline inducible pLVX-pTetOne-puro vector (Takara Bio) using In-Fusion HD kit (Takara Bio ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293T expressing either iRhom2-1-374 or wild-type iRhom2 under TET-inducible cells were generated by lentiviral infection using gene cloned into pLVX-TetOne vector (Clontech, #631849). Methodology is similar to retroviral transduction ...
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bioRxiv - Cancer Biology 2023Quote: ... pBH001 is comprised of a tetracycline-regulated bidirectional promoter for inducible expression of both firefly luciferase (a fragment cloned from the pTRE3G-BI-Luc control plasmid (Takara Bio)) and a recoded human L1 sequence (encoding both ORF1 and ORF2 ...
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bioRxiv - Cell Biology 2024Quote: ... 1999) with a retroviral vector expressing integrin β1 under a tet-inducible promotor (Retro-X™ Tet-On®; Clontech, USA). Integrin β1-induced and FACS sorted cells were cultured in DMEM with 10% fetal bovine serum ...
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bioRxiv - Biochemistry 2019Quote: ... Western blotting was performed with anti-6×His mouse mAb (Clontech), with secondary IRDye 800CW goat anti-mouse (LI-COR ...
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bioRxiv - Molecular Biology 2020Quote: ... The supernatant was incubated with TALON His-tag purification resin (TaKaRa) or glutathione agarose beads (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... 6xHis tagged proteins were purified using Talon Co2+ affinity resin (Takara Bio, USA) and eluted with 150 mM Imidazole in the lysis buffer pH 7.8 ...
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bioRxiv - Cell Biology 2022Quote: ... GFP-tagged PDE5A isoforms plasmids [19] were co-transfected with pDsRed-mito (Clontech) into HEK293 cells using Lipofectamine 3000 (Life Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... Shield-1 ligand for stabilization of DD-tagged proteins was purchased from Takara Bio (Cat # 632189 ...
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bioRxiv - Cell Biology 2023Quote: ... for GST-tagged protein or on TALON-Cobalt beads column (635507, Takara Bio) for 6XHIS-tagged protein as previously described [19,26] ...
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bioRxiv - Microbiology 2020Quote: ... and 12 U of recombinant RNase inhibitor (Takara Bio) in 1X T4 RNA Ligase Buffer (NEB) ...
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bioRxiv - Genetics 2019Quote: ... 0.25 μL Recombinant RNase-Inhibitor (40 U/μL, Clontech), 0.1μL Maxima H Minus Reverse Transcriptase (200U/μL ...
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bioRxiv - Molecular Biology 2019Quote: ... After DNase I treatment (Recombinant DNase I, TaKaRa #2270A), cDNAs were synthesized with ReverTra Ace qPCR RT Master Mix (TOYOBO ...
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bioRxiv - Microbiology 2022Quote: ... 0.8 U/μl recombinant RNase inhibitor (Takara, Cat# 2313B)] and incubated at room temperature for 10 min ...
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bioRxiv - Immunology 2020Quote: ... 1.67 U/μl Recombinant RNase Inhibitor (Takara Bio, 2313B), 1.67X First-Strand Buffer (Takara Bio ...
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bioRxiv - Neuroscience 2021Quote: ... and 0.4 U µl−1 recombinant RNase inhibitor (Clontech)) using a Wheaton Dounce tissue grinder (15 strokes with the loose pestle) ...
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bioRxiv - Microbiology 2021Quote: ... 0.8 U/μl recombinant RNase inhibitor (Takara, cat# 2313B)] and incubated at room temperature for 10 min ...
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bioRxiv - Developmental Biology 2022Quote: ... 1.67 U/μl Recombinant RNase Inhibitor (Takara Bio, 2313B), 1.67X First-Strand Buffer (Takara Bio ...
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bioRxiv - Microbiology 2021Quote: ... 0.8 U/μl recombinant RNase inhibitor (Takara, Cat# 2313B)] and incubated at room temperature for 10 min ...
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bioRxiv - Immunology 2021Quote: ... and 3 U Recombinant RNase Inhibitor (Takara, Cat#2313A), sealed and immediately frozen on dry ice ...
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bioRxiv - Immunology 2020Quote: ... After digestion with the recombinant RNase-free DNase (TaKaRa) to eliminate the genomic contamination ...
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bioRxiv - Cancer Biology 2019Quote: ... 1.67 U/µl Recombinant RNase Inhibitor (Takara Bio, 2313B), 1.67X First-Strand Buffer (Takara Bio ...
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bioRxiv - Microbiology 2023Quote: ... and 12 U of recombinant RNase inhibitor (Takara Bio) in 1X T4 RNA Ligase Buffer (NEB) ...
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bioRxiv - Plant Biology 2022Quote: ... 5 units of recombinant DNase I (TaKaRa, Kyoto, Japan), 200 units of RevertAid™ reverse transcriptase (Thermo Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... 1.67 U/µl Recombinant RNase Inhibitor (Takara Bio, 2313B), 1.67X First-Strand Buffer (Takara Bio ...
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bioRxiv - Microbiology 2022Quote: ... 0.8 U/μl recombinant RNase inhibitor (Takara, Cat# 2313B)] and incubated at room temperature for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... 0.8 U/μl recombinant RNase inhibitor (Takara, Cat# 2313B)] and incubated at room temperature for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... recombinant AAVs were purified using an AAVpro Concentrator (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 0.8 U/µl recombinant RNase inhibitor (Takara, Cat# 2313B)] and incubated at room temperature for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... 0.8 U/μl recombinant RNase inhibitor (Takara, Cat# 2313A)] and incubated at room temperature for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... 0.8 U/μl recombinant RNase inhibitor (Takara, Cat# 2313B)] and incubated at room temperature for 10 m ...
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bioRxiv - Immunology 2023Quote: The medium from transfected HEK293T cells was replaced with Opti-MEM containing 1 μM of B/B Homodimeriser (AP20187; Clontech) and incubated for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... cDNAs with the correct sequence were then subcloned into the doxycycline-inducible lentiviral vector pLVX-Tight-puro (Clontech, Mountain View CA, USA) using BamH1 and EcoR1 restriction sites ...
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bioRxiv - Cancer Biology 2023Quote: The MDM4 coding sequence (NM_002393.5) was cloned into Lenti-X™ Tet-One™ Inducible Expression System (Takara Bio, cat. no. 631847). The resulting plasmid was then subject to whole-plasmid sequencing for verification ...
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bioRxiv - Bioengineering 2024Quote: ... The plasmid pmCherry was generated by fusing the lacI-Ptrc inducible system and mCherry into the vector pBBR1MCS-5 using In-Fusion® Snap Assembly Master Mix (Takara Bio). All plasmids were extracted using QIAprep® Spin Miniprep Kit (Qiagen) ...
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bioRxiv - Microbiology 2019Quote: ... IN and CCD proteins were loaded onto a His-TALON column (TAKARA), and the columns were washed with IN wash buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2019Quote: ... The resulting fragment was cell-free cloned into pNI-His (Takara Bio) for expression in B ...