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Citations for Takara Bio :
51 - 100 of 847 citations for Recombinant Human Ribonucleotide Reductase M2 B TP53 inducible His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Doxycycline (Dox)-inducible gene-expressing cell lines were generated using Retro-X™ Tet-On 3G Inducible Expression System (Takara Bio). Plat-GP cells were transfected with the pRetroX-Tet3G plasmid together with the packaging plasmid pCMV-VSV-G using Lipofectamine 2000 ...
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bioRxiv - Neuroscience 2019Quote: SH-SY5Y cells with inducible expression of GDE2 constructs were generated using the Retro-X™ Tet-On® Advanced Inducible Expression System (ClonTech), as described (Matas-Rico et al. ...
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bioRxiv - Developmental Biology 2021Quote: Doxycycline-inducible overexpression of transcription factors was achieved using the Lenti-X Tet-On 3G Inducible Expression System (Clontech; Cat. No. 631363) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... were cloned into pRev-Tre tetracycline inducible vectors (Clontech). Kinase dead catalytically inactive versions of Drosophila and human RIOK2 (dRIOK2123A,246A ...
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bioRxiv - Microbiology 2019Quote: For the Tet-ON inducible expression plasmid pTRE3G (Clontech), WH1(WT)-TagGFP2 and TagGFP2 genes were amplified by PCR (5’-GAAGATCTATGGGCAGCCATCATCATCA and 5’-CCGGAATTCCATATGTTACCTGTACAGCCTC primers) ...
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bioRxiv - Bioengineering 2023Quote: ... GA-MatryoshCaMP6s was amplified from pRSET B His-GA-MatryoshCaMP6s and subcloned into pDONR /Zeo via In-Fusion cloning (Takara Bio ...
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bioRxiv - Molecular Biology 2023Quote: ... His (Clontech), supplemented with 1.5 mM 3-amino-1,2,4-triazol (3-AT ...
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bioRxiv - Cancer Biology 2019Quote: ... A full-length Myc tagged cDNA expressing human NEK9 (NM_001329237.1) was subcloned into the pVLX-Tight-Puro vector (Clontech). The RCC1 domain was deleted using the Quickchange® II XL Site-Directed Mutagenesis Kit according to manufacturer’s instructions (Stratagene ...
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bioRxiv - Developmental Biology 2021Quote: ... Tet-ON 3G doxycycline (Dox)-inducible lentiviral vector (Takara, 631337) was modified by inserting EF1a-TagRFP-2A-tet3G with tetON-NKX2-1 CDS ...
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bioRxiv - Cell Biology 2021Quote: ... for doxycycline-inducible expression using InFusion HD cloning kit (TaKaRa). Additional GGGS linker was introduced between the Cry2olig and caspase/kinase domains or Cry2olig and mCherry to reduce potential sterical interference between domains ...
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bioRxiv - Cancer Biology 2022Quote: ... ATF4 cDNA was cloned into a doxycycline inducible pLVX (Clontech) vector ...
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bioRxiv - Systems Biology 2019Quote: ... Lenti-X™ Tet-One™ Inducible Expression System (Clontech) was used ...
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bioRxiv - Cell Biology 2021Quote: Inducible gene expression system based on Tet-On 3G (Clontech) was employed ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA was utilized with the SMARTer Human TCR a/b Profiling Kit v2 (Takara, USA). The resulting libraries were sequenced for paired-end reads of 2×250 bp on an Illumina system at a sequencing depth of 7.5X.
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bioRxiv - Developmental Biology 2021Quote: ... –His (630428, Clontech) or –Trp ...
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bioRxiv - Developmental Biology 2021Quote: ... –His (630419, Clontech) selective media +3 mM ...
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bioRxiv - Plant Biology 2022Quote: ... and His (Clontech) with and without the addition of 3-Amino-1H-1,2,4-triazole (Acros Organics (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: PDGF-IRES-Cre was generated by cloning human PDGF-B and Cre into pQXIX vector (Clontech) as previously described5 ...
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bioRxiv - Cell Biology 2021Quote: ... pDAA-002 encodes a C-terminal FLAG-tagged version of human PKR hosted in the retroviral expression vector pLPCX (Clontech) and was generated by cloning a PCR product (PCRP ...
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bioRxiv - Biochemistry 2023Quote: ... FL-human KANK1 (generous donation from the Bershadsky lab) cDNA was tagged in the C-terminal site with pmCherry (Clontech) by restriction digestion ...
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bioRxiv - Cell Biology 2020Quote: Sequences of full length hsRIPK2 with an N-terminal 3x-Flag tag were synthesized by Genscript and cloned into doxycycline-inducible lentiviral expression vectors (pF_TRE3G_rtTAAd_puro (Takara Bio)) ...
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bioRxiv - Cell Biology 2023Quote: ... Tet-On Inducible Expression System® plasmid (Clontech, Cat.No. 1. 634301) was linearized by digestion with EcoRI and AgeI ...
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bioRxiv - Developmental Biology 2021Quote: ... AP20187 (B/B) compound (Takara Bioscience) was injected into the dorsal aorta through a small window made in the ventral egg ...
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bioRxiv - Biophysics 2022Quote: ... B/B homodimeriser (AP20187, Takara Bio), dibenzocyclooctyne-PEG4-maleimide (760676 ...
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bioRxiv - Cell Biology 2021Quote: ... the coding sequences of human Pcnt B and Pcnt S were subcloned into peGFP-N1 (Takara Clontech) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Cell Biology 2021Quote: ... the coding sequences of human Pcnt B and Pcnt S were subcloned into peGFP-N1 (Takara Clontech) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: ... anti-His (Clontech 631212), anti-H3K36me2 (Upstate 07-369) ...
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bioRxiv - Cell Biology 2023Quote: ... Cre recombinase proteins were delivered into TP53-missense mutation knock-in cells using Cre Recombinase Gesicles (Takara Bio).
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bioRxiv - Synthetic Biology 2022Quote: ... B/B dimerizer (AP20187 ligand; TaKaRa 635058) was added to a final concentration of 500 nM in the well ...
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bioRxiv - Immunology 2022Quote: ... B/B homodimerizer (Takara Bio, Cat#635058), b-estradiol (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... 500 nM B/B Homodimerizer (Takara, #635058) was also added ...
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bioRxiv - Neuroscience 2023Quote: B/B homodimerizer was purchased from Takara USA lnc ...
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bioRxiv - Molecular Biology 2024Quote: ... The N-terminal His tag construct of human MPST was co-transformed with GroES-EL chaperon plasmid from Takara (#3340), overexpressed in E ...
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bioRxiv - Cancer Biology 2020Quote: ... we used the Lenti-X TET-ON Advanced Inducible Expression System (Clontech), which would allow controlled expression of FOXL2WT or FOXL2C134W protein by addition of Dox (Sigma ...
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bioRxiv - Immunology 2020Quote: ... Doxycycline inducible expression was achieved by pLVX-TetON-Advanced (Takara Bio, 632162) expression.
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bioRxiv - Cancer Biology 2022Quote: ... then cloned into the doxycycline inducible pLVX-pTetOne-puro vector (Takara Bio) using In-Fusion HD (Takara Bio ...
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bioRxiv - Cell Biology 2023Quote: ... To introduce Tet-ON system (Tets-On inducible system, Takara, Madison, WI), a set of two plasmids were introduced to bax-/-bak-/- MEFs to establish each cell line ...
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bioRxiv - Pathology 2020Quote: AP21087 ((B/B homodimerizer, Takara Bio, CA, USA) was administered by intra-peritoneal (ip ...
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bioRxiv - Cell Biology 2019Quote: ... AP20187 (B/B Homodimerizer) was purchased from Takara. The recombinant Anti-M1 Spastin rabbit monoclonal antibody ...
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bioRxiv - Molecular Biology 2019Quote: ... with Not1/Mlu1 for doxycycline inducible expression with the Tet-On Advanced (Clontech) system ...
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bioRxiv - Systems Biology 2021Quote: ... the inducible fluorescent protein was induced by adding 1 μg/ml doxycycline (Clontech) alone or together with 100 nM rapamycin (Harveybio ...
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bioRxiv - Cell Biology 2021Quote: ... was purchased from Addgene and the iDimerize™ Inducible Homodimer System (Cat # 635068) was purchased from Clontech Laboratories ...
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bioRxiv - Cell Biology 2019Quote: ... we employed the Retro-X Tet-On advanced inducible expression system (Takara Bio) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... the repeat insert was further subcloned to a doxycycline-inducible (Tet-On, Clontech) expression vector containing 12x MS2 hairpins.
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bioRxiv - Synthetic Biology 2022Quote: ... 500 nM of the B/B dimerizer (TaKaRa 635058) was added 2 hours before the start of the assay (or an Ethanol vehicle control was added) ...
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bioRxiv - Immunology 2019Quote: ... B/B Homodimerizer (Takara, 635059, equivalent to AP-20187), disuccinimidyl suberate (DSS ...
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bioRxiv - Neuroscience 2022Quote: ... AP20187 (B/B) (Clontech, Saint-Germain-en-Laye France) was added 4 h post-transfection overnight.
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bioRxiv - Immunology 2024Quote: ... genes encoding the variable regions of C7 and C74 heavy chains were cloned into a pMN vector with a human CH1 domain and a C-terminal His-tag using In-Fusion cloning system (Takara Bio #639649). The full light chains of C7 and C74 were cloned into the pMN vector without any purification tag using the same method ...
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bioRxiv - Developmental Biology 2023Quote: ... Interactions were evaluated on agar that was -His or -His -Ade (Clontech, now Takara Bio). Growth in the absence of either supplement indicates transcription from reconstituted GAL4 proteins ...
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bioRxiv - Developmental Biology 2023Quote: ... Interactions were evaluated on agar that was -His or -His -Ade (Clontech, now Takara Bio). Growth in the absence of either supplement indicates transcription from reconstituted GAL4 proteins ...