Labshake search
Citations for Takara Bio :
1901 - 1950 of 6736 citations for Onion Yellow Dwarf Virus OYDV PCR Kits since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Evolutionary Biology 2023Quote: ... Mycoplasma contamination in cell cultures was routinely tested using the PCR mycoplasma detection set (Takara Bio). At approximately 70% confluence ...
-
bioRxiv - Plant Biology 2024Quote: ... The SKI2 cDNA sequence was amplified from wild type cDNA using CloneAmp HiFi PCR Premix (Takara) and cloned into the pPZP212 binary vector ...
-
bioRxiv - Immunology 2024Quote: ... Real-Time PCR was performed by using specific primers and SYBR Green ROX Mix (RR420A, Takara). β-actin was used as the internal quantitative control.
-
bioRxiv - Microbiology 2024Quote: ... The HA1 subunit was divided into three fragments for PCR (PrimeSTAR Max DNA Polymerase, Takara Bio), seven Ns and partial sequencing adapters were added in the primer as the barcode ...
-
bioRxiv - Biochemistry 2024Quote: The SUGCT coding sequence was amplified by PCR by using PrimeStar GXL polymerase (Takara Bio Inc) using the following primers (start and stop codon in bold) ...
-
bioRxiv - Neuroscience 2023Quote: ... 5 μl of the PCR product was treated with 2 μl cloning enhancer (Takara-bio Inc.). The cloning reaction was prepared by adding 2 μl of 5X In-Fusion HD Cloning enzyme mix ...
-
bioRxiv - Cancer Biology 2023Quote: ... a region spanning the ENE domain was amplified by conventional PCR (PrimeStar HS mix, Takara Bio) with primer sequences listed in Table S9 ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: PCR reactions were performed using PrimeSTAR® Max DNA Polymerase (TAKARA Bio Inc., San Jose, CA) according to manufacturer protocols ...
-
bioRxiv - Genomics 2023Quote: ... while the out/out PCRs were carried out with PrimeSTAR GXL DNA Polymerase (Takara Bio, R051A). Seamless HDR was confirmed by sequencing the junctions between the insert and the mouse genome ...
-
bioRxiv - Immunology 2023Quote: ... qRT-PCR was performed using the SYBR® Premix Ex Taq™ II (Takara, Shiga, Japan) Kit and a StepOnePlus Real-Time PCR instrument ...
-
bioRxiv - Immunology 2023Quote: ... The viral copies were quantitated by reverse transcription quantitative PCR following the handbook (Takara, Cat.#RR086A). The copy numbers of reverse-transcripts from cell cultures and mice organ samples were normalized with the expression level housekeeping gene of beta-actin by using the 2(-Delta Delta C(T) ...
-
bioRxiv - Cell Biology 2023Quote: ... Quantitative real-time PCR was performed in Thermal Cycler Dice Real Time System Lite (Takara Bio) using KOD SYBR qPCR Mix (Toyobo ...
-
bioRxiv - Plant Biology 2023Quote: ... The full-length CAMTA3 cDNA was PCR amplified using the HS Primestar DNA polymerase (Takara, USA) with forward and reverse primers bearing BamHI and XhoI ...
-
bioRxiv - Genetics 2023Quote: ... Reverse transcription and quantitative real-time PCR were performed with PrimeScriptTM RT Master Mix (Takara, RR037A) and TB Green Premix Ex TaqTM II (Takara ...
-
bioRxiv - Molecular Biology 2023Quote: ... qRT-PCR was performed using the Thermal Cycler Dice Real Time System (Takara Bio, Kusatsu, Japan). Primers and templates were mixed with TB Green Premix Ex Taq II (Takara Bio) ...
-
bioRxiv - Cancer Biology 2023Quote: ... PCR for selection cassette production was performed with SeqAmp DNA Polymerase (Takara Bio, cat. no. 63850) using the ultramers as primers and AAT-PB-CD2APtk (Addgene #86004 ...
-
bioRxiv - Developmental Biology 2023Quote: ... PCR condition modifications to amplify RACE products are as followed using SeqAmp DNA Polymerase (Takara Bio): Fat1 (TA ...
-
bioRxiv - Cell Biology 2023Quote: ... RT-PCR was performed with a Takara Thermal Cycler Dice® Touch (Takara Bio, Kusatsu, Japan). The RT-PCR program was as follows ...
-
bioRxiv - Cell Biology 2023Quote: ... Second strand synthesis and PCR amplification was performed by adding the Advantage 2 Polymerase Mix (Clontech) and the SINGV6 primer (10 pmol ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... PCR was performed using TaKaRa PrimeSTAR GXL DNA Polymerase following the manufacturer’s protocol (Takara Biotechnology, Japan). Amplified DNA fragments were purified by illustra ExoProStar (GE Healthcare Life Sciences) ...
-
bioRxiv - Plant Biology 2023Quote: The truncated version of the RPF2nad6 was generated by PCR amplification using Takara PrimeStar polymerase (TaKaRa) and a primer pair designed to remove the last 180 nucleotides of the RPF2nad6 coding sequence representing the RfCTD sequence (Supplementary table 6) ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... and deposited into a PCR tube with 5 μL lysis buffer (Takara Bio, San Francisco, CA). cDNAs were then prepared by reverse transcriptase (Takara Bio ...
-
bioRxiv - Microbiology 2023Quote: ... Amplifying PCR of the first stand cDNA product was then performed using SeqAmp DNA Polymerase (Takara) with a nested 3’ primer to the constant genes and a 5’ universal primer based on universal primer sites added to the 5’ end during cDNA generation ...
-
bioRxiv - Cell Biology 2024Quote: The PCR mixtures for the reactions described here were composed using PrimeStar (Takara Bio, Shiga, Japan) with a 2X GC buffer ...
-
bioRxiv - Plant Biology 2024Quote: ... The obtained cDNA was subjected to 5’ RACE-PCR using SeqAmp DNA Polymerase (Takara Bio USA). Primers used for 5’ RACE-PCR and the number of PCR cycles are shown in Supplemental Table 3 ...
-
bioRxiv - Plant Biology 2024Quote: ... and amplified by PCR using Takara Premix Ex TaqTM Hot Start Version (Takara Research, RR030A, Japan). The amplified products were recovered from an agarose gel using a Universal DNA Purification Kit (TIANGEN ...
-
bioRxiv - Developmental Biology 2024Quote: ... Full-length cDNA fragment of human MAK was amplified by PCR using human retinal cDNA (Clontech) as a template and subcloned into the pCAGGSII-2xHA vector (62) ...
-
bioRxiv - Synthetic Biology 2024Quote: ... DNA fragments were amplified using PCR using PrimeSTAR® Max DNA Polymerase (Takara Biomedical Technology (Beijing) Co. ...
-
bioRxiv - Plant Biology 2024Quote: ... CYP71B130 and CYP71B131a coding sequences (CDSs) were PCR-amplified using the PrimeSTAR® Max polymerase (Takara), according to supplier’s recommendations ...
-
bioRxiv - Molecular Biology 2024Quote: ... Second strand synthesis and PCR amplification was done by adding the Advantage 2 Polymerase Mix (Clontech) and the SINGV6 primer (10 pM ...
-
bioRxiv - Microbiology 2024Quote: ... PCR products were inserted into the respective plasmids using In-Fusion Snap assembly Master mix (Takara). All restriction enzymes used in this study were purchased from New England Biolabs ...
-
Emerging cooperativity between Oct4 and Sox2 governs the pluripotency network in mouse early embryosbioRxiv - Developmental Biology 2024Quote: ... cDNA was amplified with V3d(T)24 and R2SP primers and Terra PCR Direct Polymerase (Clontech) for 16 cycles ...
-
bioRxiv - Plant Biology 2024Quote: ... was amplified from Tak-1 genomic DNA by PCR using PrimeSTAR Max (Takara Bio Inc., Japan) with the primers IF-MpRLFpro-F and IF-MpRLFpro-R ...
-
bioRxiv - Molecular Biology 2024Quote: ... The extracted genomic DNA was used to perform PCR with high-fidelity polymerase (Takara Bio Inc) to confirm the transgene expression by Sanger sequencing.
-
bioRxiv - Molecular Biology 2024Quote: ... The resulting PCR product was subsequently cloned and inserted into the pMD20-T vector (TaKaRa Bio) using the Mighty TA-cloning Kit (TaKaRa Bio ...
-
Decreased Astrocytic CCL5 by MiR-324-5p Ameliorates Ischemic Stroke Injury via CCR5/ERK/CREB PathwaybioRxiv - Neuroscience 2024Quote: ... and RT-PCR amplification of miRNA was performed using the TB Green Advantage qPCR Premix (TaKaRa). mRNA levels and miRNA levels were normalized to Gapdh and U6 ...
-
Decreased Astrocytic CCL5 by MiR-324-5p Ameliorates Ischemic Stroke Injury via CCR5/ERK/CREB PathwaybioRxiv - Neuroscience 2024Quote: ... RT-PCR amplification of mRNA was performed using the TB Green Premix Ex Taq II (Takara), and RT-PCR amplification of miRNA was performed using the TB Green Advantage qPCR Premix (TaKaRa) ...
-
bioRxiv - Cell Biology 2024Quote: ... The purified PCR product was used for an In-Fusion reaction (Takara Bio Inc. Shiga, Japan) with XhoI and BamHI linearized pTurbo vector (Evrogen ...
-
bioRxiv - Immunology 2024Quote: ... The cDNA was subjected to quantitative PCR performed by using TB Green Premix Ex Taq (Takara) in Thermal Cycler Dice Real Time System (Takara) ...
-
bioRxiv - Bioengineering 2024Quote: ... and WPRE-SV40 poly A signal-loxP21 were PCR-amplified with PrimeSTAR Max DNA polymerase (Takara). The N-terminus of dPspCas13b and the C-terminus of ADAR2DD were tethered with the bipartite nuclear localization signal sequence to facilitate nuclear translocation ...
-
bioRxiv - Cell Biology 2024Quote: ... The amplified PCR products were digested with restriction enzymes and inserted into EGFP-C1 vector (Clontech).
-
bioRxiv - Cell Biology 2024Quote: ... Quantitative real-time PCR (qPCR) was performed using the TB Green Premix Ex Taq (Takara, #RR420A) on the QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) ...
-
bioRxiv - Cell Biology 2024Quote: ... Gibson fragments were amplified by polymerase chain reactions (PCRs) using either the PrimeSTAR GLX (R050A, Takara), LA Taq Hot Start (RR042A ...
-
bioRxiv - Cell Biology 2020Quote: ... FAM111A cDNAs were PCR amplified and inserted into destination vectors using In-Fusion seamless cloning (Takara Bio). The pTRIPz-MycBioID vector allows for doxycycline-inducible expression of RNF4 or FAM111A with N-terminal fusion of a Myc tag and a promiscuous biotin ligase (MycBioID ...
-
bioRxiv - Developmental Biology 2021Quote: ... 2015) with MluI and inserting PCR-amplified EGFP from pEGFP-C1 (Clontech Laboratories, Mountain View, CA, USA). Tol2-mCherry was produced by digesting Tol2-EGFP-C1 with NheI/EcoRI to remove GFP and inserting mCherry amplified by PCR from 8xGliBS-IVS2-mCherry-NLS-polyA-Tol2 (a gift from James Chen (Addgene plasmid #84604) ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... or with standard (DreamTaq - Thermo Fisher Scientific, or EmeraldAmp Max HS PCR Master Mix - TaKaRa Bio Inc) DNA polymerases ...
-
bioRxiv - Plant Biology 2020Quote: ... The qRT-PCR reaction mixture included TB Green® Premix Ex Taq™ II (Takara, Shiga, Japan). The qRT-PCR conditions were as follows ...
-
bioRxiv - Molecular Biology 2020Quote: ... The first PCR products were subsequently amplified by the nested Per2AS specific primers with Universal Primer (Clontech). The nested PCR products were cloned into pGEM-T vector (Promega ...
-
bioRxiv - Neuroscience 2020Quote: ... PCR amplification of the vectors and the FPs was performed using CloneAmp™ DNA polymerase (Clontech, USA). The PCR products were gel purified (Macherey-Nagel ...
-
bioRxiv - Biochemistry 2020Quote: ... were replaced by the BG505-NxT or BG505-NxS PCR fragments using the In-Fusion enzyme (Clontech) as described by the manufacturer ...