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Citations for Takara Bio :
1851 - 1900 of 6736 citations for Onion Yellow Dwarf Virus OYDV PCR Kits since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The PCR-amplified elements were ligated in a single reaction step by In-Fusion cloning (Clontech) upstream of the HA3 tag in XmaI and AfeI sites of pLN-HA3-loxP ...
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bioRxiv - Cell Biology 2021Quote: ... PCR amplification were conducted using Ex Taq in accordance with the manufacturer’s instructions (TaKaRa Bio, Inc.).
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bioRxiv - Cell Biology 2021Quote: ... The real-time quantitative PCR analysis was performed using SYBR Premix Ex Taq II (TAKARA, China) on LightCycler 480 PCR System (Roche ...
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bioRxiv - Molecular Biology 2022Quote: ... EBIV M/L segment-specific sequences were amplified by PCR including Universal Primer A Mix (Takara), Gene-Specific Primers (GSPs ...
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bioRxiv - Physiology 2022Quote: ... PCR reaction was conducted using SYBR qPCR Premix Ex Taq II Tli RNase H+ (TAKRR820W, Takara). Primer pairs are listed in supplementary table S2.
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bioRxiv - Microbiology 2022Quote: ... The PCR product and pET28b plasmid were digested with restriction endonuclease EcoRI and HindIII (Japanese Takara), then ligated with T4 DNA Ligase (Japanese Takara) ...
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bioRxiv - Microbiology 2022Quote: ... Amplifying PCR of the first stand cDNA product was then performed using SeqAmp DNA Polymerase (Takara) with a nested 3’ primer to the constant genes and a 5’ universal primer based on universal primer sites added to the 5’ end during cDNA generation ...
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bioRxiv - Microbiology 2022Quote: ... GISAID ID: EPI_ISL_408667)29 were prepared by PCR using PrimeSTAR GXL DNA polymerase (Takara, Cat# R050A). A linker fragment encoding hepatitis delta virus ribozyme ...
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bioRxiv - Genetics 2022Quote: ... Each 30 µL PCR reaction volume contained 15 µL Premix Ex Taq enzyme (Takara Biomedical Technology), 0.2 µL BSA ...
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bioRxiv - Genetics 2022Quote: ... The 20 µL PCR reaction volume included 10 µL Premix Ex Taq enzyme (Takara Biomedical Technology), 0.2 µL BSA ...
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bioRxiv - Cell Biology 2022Quote: ... and each reaction contained 1 μl of cDNA and the SYBR Green PCR mix (Takara, RR420A), to which gene-specific forward and reverse PCR primers were added ...
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bioRxiv - Microbiology 2020Quote: ... were amplified by PCR using genome as template and cloned into T-Vector pMD19 (Simple, Takara). Primers containing the mutations were used to PCR the whole derived pMD19 plasmid constructed above ...
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bioRxiv - Immunology 2020Quote: ... real-time PCR analysis was carried out using Premix Ex Taq (probe qPCR) (Takara, Dalian, China). Real-time PCR was performed with WSSV-specific primers WSSV-RT1 (5’-TTGGTTTCATGCCCGAGA TT-3’ ...
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bioRxiv - Microbiology 2021Quote: ... GISAID ID: EPI_ISL_408667)37 were prepared by PCR using PrimeSTAR GXL DNA polymerase (Takara, cat# R050A). A linker fragment encoding hepatitis delta virus ribozyme ...
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bioRxiv - Genomics 2022Quote: ... and post-capture PCR amplification was performed for 16-18 cycles using PrimeSTAR GXL Polymerase (Takara) at an annealing temperature of 60°C.
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bioRxiv - Molecular Biology 2020Quote: ... The qRT-PCR analyses were performed using SYBR Premix ExTaq TM II Mix (TaKaRa, Shiga, Japan). Actin3 (GenBank accession no. ...
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bioRxiv - Neuroscience 2020Quote: ... PCR amplified and inserted into the DsRed-Express2 plasmid using in-fusion cloning (Takara Bio, CA). St3-Halo was generated by replacing the GFP in St3-eGFP with Halo tag (GenScript USA Inc ...
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bioRxiv - Genetics 2021Quote: Isolated RNA from OSF and control tissues was immediately subjected to realtime RT PCR (using Takara PrimescriptTM one step realtime RT PCR kit ...
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bioRxiv - Genetics 2020Quote: ... 356-600bp regions encompassing the SNP of interest were amplified using CloneAmp HiFi PCR Premix (Clontech), and then cloned into the pGL4.23-mini/P vector including a minimal SV40 promoter upstream of firefly luciferase ...
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bioRxiv - Cell Biology 2020Quote: ... gDNA of CFU colonies was extracted by suspending the cell in 1×PCR buffer (Takara, R050A) containing 0.2mg/ml proteinase K (Tiangen ...
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bioRxiv - Cell Biology 2022Quote: ... TurboGFP was exchanged for eGFP by amplifying the eGFP sequence by PCR from peGFP-N1 (Clontech) using the following primers to introduce AgeI and NotI restriction sites ...
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bioRxiv - Cell Biology 2021Quote: ... the full-length Borr open reading frame was amplified from LD36125 by PCR using PrimeStar (Takara) and cloned between AscI and NotI sites of pENTR (ThermoFisher ...
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bioRxiv - Physiology 2021Quote: ... Second strand synthesis and PCR amplification was done by adding the Advantage 2 Polymerase Mix (Clontech) and the SINGV6 primer (10 pmol ...
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bioRxiv - Plant Biology 2022Quote: DNA was amplified from cDNA by PCR using PrimeStar GXL DNA polymerase (Takara Bio, Shiga, Japan) and verified by DNA sequencing using a 3130 Genetic Analyzer (Applied Biosystems ...
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bioRxiv - Immunology 2020Quote: ... Quantitative PCR was performed using TB Green® Premix Ex Taq™ II (Takara, Tokyo, Japan). The siRNA was purchased from Dharmacon and transfected into Raw and PM cells using the DharmaFECT 1 siRNA Transfection Reagent.
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bioRxiv - Immunology 2021Quote: ... Quantitative real-time polymerase chain reaction (PCR) was performed using TB Green Premix Taq II (TaKaRa) and a StepOnePlus Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Evolutionary Biology 2021Quote: Amplified with V4 16S rRNA primers as above using EmeraldAmp GT PCR Master Mix (TAKARA BIO).
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bioRxiv - Immunology 2020Quote: ... EqPD-1 and EqPD-L1 cDNAs were amplified by PCR using TaKaRa Ex Taq (Takara Bio) and specific primers (Supplementary Table 1) ...
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bioRxiv - Immunology 2021Quote: ... which involves the following steps: i) cDNA amplification (Terra PCR direct Polymerase, Takara, cat. no. 639270), ii ...
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bioRxiv - Immunology 2021Quote: ... using the modified (Switching Mechanism At 5’ End of RNA Transcript) PCR cDNA synthesis protocol (Clontech) and oligonucleotides as described below (Table S3) ...
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bioRxiv - Genomics 2021Quote: ... according to the instructions of SYBR®Green Real-time PCR Master Mix (Takara, Dalian, China). Reaction conditions were 95°C for 5 min ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR was performed with Advantage-HD DNA polymerase (Takara Bio, Saint-Germain-en-Laye, France) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... and quantitative PCR (qPCR) was performed to detect EGFR levels by using SYBR-Green dye (Takara). Further ...
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bioRxiv - Neuroscience 2021Quote: ... rat Txn1 cDNAs were amplified by PCR using LA-Taq polymerase (Takara Bio Inc, Shiga, Japan) with FW-primer ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: PCR reactions were performed using PrimeSTAR® Max DNA Polymerase (TAKARA Bio Inc., San Jose, CA) according to manufacturer protocols ...
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bioRxiv - Neuroscience 2023Quote: ... 5 μl of the PCR product was treated with 2 μl cloning enhancer (Takara-bio Inc.). The cloning reaction was prepared by adding 2 μl of 5X In-Fusion HD Cloning enzyme mix ...
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bioRxiv - Cancer Biology 2023Quote: ... a region spanning the ENE domain was amplified by conventional PCR (PrimeStar HS mix, Takara Bio) with primer sequences listed in Table S9 ...
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bioRxiv - Genetics 2023Quote: ... Reverse transcription and quantitative real-time PCR were performed with PrimeScriptTM RT Master Mix (Takara, RR037A) and TB Green Premix Ex TaqTM II (Takara ...
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bioRxiv - Molecular Biology 2023Quote: ... qRT-PCR was performed using the Thermal Cycler Dice Real Time System (Takara Bio, Kusatsu, Japan). Primers and templates were mixed with TB Green Premix Ex Taq II (Takara Bio) ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative real-time PCR was performed in Thermal Cycler Dice Real Time System Lite (Takara Bio) using KOD SYBR qPCR Mix (Toyobo ...
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bioRxiv - Plant Biology 2023Quote: ... The full-length CAMTA3 cDNA was PCR amplified using the HS Primestar DNA polymerase (Takara, USA) with forward and reverse primers bearing BamHI and XhoI ...
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bioRxiv - Genomics 2023Quote: ... while the out/out PCRs were carried out with PrimeSTAR GXL DNA Polymerase (Takara Bio, R051A). Seamless HDR was confirmed by sequencing the junctions between the insert and the mouse genome ...
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bioRxiv - Immunology 2023Quote: ... qRT-PCR was performed using the SYBR® Premix Ex Taq™ II (Takara, Shiga, Japan) Kit and a StepOnePlus Real-Time PCR instrument ...
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bioRxiv - Immunology 2023Quote: ... The viral copies were quantitated by reverse transcription quantitative PCR following the handbook (Takara, Cat.#RR086A). The copy numbers of reverse-transcripts from cell cultures and mice organ samples were normalized with the expression level housekeeping gene of beta-actin by using the 2(-Delta Delta C(T) ...
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bioRxiv - Neuroscience 2022Quote: ... Target sequences were amplified by 18–30 cycles of PCR using Ex-Taq™ (Takara Bio). For semiquantitative PCR ...
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bioRxiv - Molecular Biology 2022Quote: We amplified Complementary DNAs (cDNAs) of genes by PCR using PrimeSTAR Max DNA polymerase (TAKARA Bio), and cloned the PCR products into overexpression vectors EGFP-N1 (Addgene ...
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bioRxiv - Plant Biology 2024Quote: ... The SKI2 cDNA sequence was amplified from wild type cDNA using CloneAmp HiFi PCR Premix (Takara) and cloned into the pPZP212 binary vector ...
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bioRxiv - Molecular Biology 2024Quote: ... All constructs were cloned using PCR amplification of complimentary fragments and In-Fusion HD assembly (Takara). pRRL-hPGK-mCherry-P2A-PuroR-T2A-FLAG-APEX2 was created by PCR amplifying hPGK-mCherry-P2A-PuroR-T2A from a synthesised gene fragment purchased from GeneWiz and APEX2 from pcDNA3-FLAG-APEX2-NES (a gift from Alice Ting67 ...
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bioRxiv - Neuroscience 2023Quote: ... AAV was titrated using quantitative PCR (qPCR) with primers for the ITR sequence (TaKaRa Bio Inc).
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bioRxiv - Neuroscience 2024Quote: ... The titer of AAV was determined with a real-time PCR thermal cycler (Dice, Takara Bio). The resultant AAV1-Syn-mCherry (2.8 × 1012 GC/ml) ...