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Citations for Takara Bio :
1901 - 1950 of 5307 citations for Mouse Putative Phospholipase B Like 2 PLBD2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... Klotho genotypes were determined by semi-quantitative PCR using LA-Tag DNA polymerase and TaKaRa buffer with Mg+2 (TaKaRa Shuzo, Tokyo, Japan) as follows ...
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bioRxiv - Cell Biology 2020Quote: ... for 1 h at 4 °C with rotation and then transferred to gravity columns (Talon® 2 ml Disposable Gravity Column, Clontech, #635606-CLI). The lysate was drained and the beads were washed five times with cold wash buffer (50 mM Tris ...
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bioRxiv - Neuroscience 2020Quote: ... 341F (5’-CCTACGGGNGGCWGCAG-3’) and 805R (5’-GACTACHVGGGTATCTAATCC-3’) or (2) 341F (5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG-3’) and 806R (5’-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGGACTACHVGGGTWTCTAAT-3’) (TaKaRa Bio, Shiga, Japan). The second PCR was performed to add the index sequences for Illumina sequencing with barcode sequences ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The SARS-CoV-2 viral load was quantified in culture supernatant using RT-QPCR with primer probes targeting E gene of SARS-CoV-2 using PrimeDirect Probe RT-qPCR Mix (TaKaRa Bio USA, Inc) and Applied Biosystems QuantStudio3 real-time PCR system (Applied Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... containing a Kozak sequence added right before the ATG (RefSeq NC_045512.2) were synthesized by Eurofins (Ebersberg, EU) and subcloned into the pIRES2 vector with eGFP in the second cassette (Takara Bio Europe, EU). Truncated Δ4 and Δ8 E proteins ...
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bioRxiv - Neuroscience 2023Quote: ... were transiently cotransfected with a 2:1 mixture of Nav1.7-expressing plasmid8 and a pIRES2-ZsGreen bicistronic plasmid (Takara Bio, San Jose, CA) expressing ZsGreen and mouse FHF2B proteins.10 The same pIRES2-ZsGreen plasmid without FHF2 coding sequence served as the control ...
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bioRxiv - Genetics 2022Quote: ... reverse transcription was carried out using 2 μL of RNA mixed with 4 μL of 5×PrimeScript IV cDNA Synthesis Mix (Takara, Code No. 6215A) containing PrimeScript IV RTase ...
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bioRxiv - Microbiology 2023Quote: ... and was then incubated with 2 mL TBS pH 8.0 pre-equilibrated Talon® Metal Affinity resin during 2 h at 4°C with permanent mixing (Takara Bio, Kusatsu, Japan). The resin was washed with 10 volumes of TBS pH 8.0 buffer containing 0.1% Triton X-100 and 10 mM imidazol (both from Sigma) ...
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bioRxiv - Biochemistry 2024Quote: ... Sub-library specific primers were then used to amplify 1 ul of cDNA template for 24 cycles using Advantage 2 PCR Mix (Takara Bio, Cat. 639206). Finally ...
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bioRxiv - Genetics 2024Quote: ... PCR was conducted as described earlier (section: RNAi-mediated knockdown of Pmtra-2) but using the proofreading PrimeSTAR HS DNA Polymerase (Takara Bio, Shiga, Japan) and Ex Taq DNA Polymerase (Takara Bio ...
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bioRxiv - Evolutionary Biology 2024Quote: ... adhaerens yeast 2-hybrid cDNA library was constructed using the Make Your Own “Mate & PlateTM” Library System (Takara Bio USA, Mountain View, CA), using whole animal total RNA extracted from approximately 30 animals using a Nucleospin RNA Plus Mini Kit (Macherey-Nagel ...
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bioRxiv - Plant Biology 2020Quote: ... according to the manufacturer’s instructions (SMARTer® RACE 5’/3’ Kit, Clontech, USA), with gene specific primers (YFT1-GSP5′-R/GSP3′-F ...
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bioRxiv - Plant Biology 2020Quote: ... using a TB Green Premix EX Taq II (Tli RNaseH Plus) Kit (Takara). The calculation method for relative gene expression levels were performed as described previously (Li et al. ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized using a combination of SMARTer PCR cDNA Synthesis Kit (Takara) and SuperScript II Reverse Transcriptase (ThermoFisher) ...
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bioRxiv - Plant Biology 2021Quote: ... Cloning procedure was performed using In-Fusion HD cloning kit (Takara Bio USA). Constructs for GFP and mCherry upstream or downstream of the Gateway cassette were amplified using primers GW_RfA_mCherry-F ...
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: ... Eluted RNA was used for library preparation using SMARTer smRNA-Seq Kit (Takara), with following modifications ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA was converted to cDNA using the PrimeScript RT reagent Kit (Takara, RR037A).
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bioRxiv - Cancer Biology 2021Quote: ... and PrimeSTAR ® Max DNA Polymerase kits according to manufacturer instructions (Takara, R045A). The PCR product was cloned into the pENTR4 no ccDB plasmid (Addgene #17424 ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA-seq libraries were prepared with SMART-Seq® Stranded Kit (Takara-Clontech). Samples were sequenced in wholeRNAseq using the NextSeq illumina 500 Platform (75 bp single read) ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA-seq libraries were prepared with SMART-Seq® Stranded Kit (Takara-Clontech). Samples were sequenced in wholeRNAseq using the NextSeq illumina 500 Platform (75 bp single read) ...
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bioRxiv - Developmental Biology 2020Quote: ... and mRNA was captured and amplified with the Smart-seq2 v4 kit (Clontech). After AMPure XP beads purification ...
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bioRxiv - Cell Biology 2020Quote: ... After cDNA was synthesized using the PrimeScript RT Reagent Kit (Takara, Dalian, China) and measured using a CFX96™ real-time PCR detection system (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... DNA assemblies were conducted with the In-Fusion HD Cloning Kit (Takara BIO) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... and cloning was performed using the In-Fusion HD cloning kit (Takara Bio) or restriction/ligation dependent cloning ...
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bioRxiv - Genetics 2021Quote: ... Reverse transcription was performed using PrimeScript II 1st strand cDNA Synthesis Kit (TAKARA) following the manufacture’s protocol ...
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bioRxiv - Genetics 2021Quote: ... Site-directed mutagenesis was performed with the In-Fusion HD kit (Clontech Inc.), creating G34D mutation ...
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bioRxiv - Molecular Biology 2021Quote: ... Ligations were performed using T4 DNA ligase (Fermentas) or InFusion HD kits (Clontech). Small-scale isolation of plasmid DNA was performed with Mini-Prep kits (Qiagen) ...
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bioRxiv - Developmental Biology 2021Quote: ... The final BAC was purified with Nucleobond BAC 100 Kit (Takara Bio, 740579) and co-injected with 1 nl of 40 ng/ul tol2 mRNA into one-cell stage embryos ...
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bioRxiv - Developmental Biology 2021Quote: ... The final BAC was purified with Nucleobond BAC 100 Kit (Takara Bio, 740579) and co-injected with 1 nl of 40 ng/ul tol2 mRNA into one-cell stage embryos ...
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bioRxiv - Developmental Biology 2021Quote: ... titration of AAV6 particles was performed using the AAVpro titration kit (TAKARA BIO).
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bioRxiv - Evolutionary Biology 2020Quote: ... The cDNA was synthesized using SMARTer® PCR cDNA Synthesis Kit (Clontech, USA) which was then amplified using the KAPA HiFi PCR Kits (Roche ...
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bioRxiv - Plant Biology 2022Quote: ... and subjected to reverse-transcription using a PrimeScript™ RT Reagent Kit (Takara Biotechnology ...
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bioRxiv - Neuroscience 2022Quote: ... for QF2 lines and NucleoBond Xtra Midi Endotoxin-Free plasmid kit (Clontech 740420.50) for Split-QF2 lines and eluted in ultrapure water prior to injection ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized using a PrimeScript® RT Reagent Kit (TaKaRa, Shiga, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... to extract RNA and prepare cDNA libraries using SMARTseq v4 kit (Clontech, # 634888) and sequenced using HiSeq 2500 in 100 pairbase length fragments for a minimum of 1 million reads per sample ...
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bioRxiv - Molecular Biology 2021Quote: ... Libraries were generated using the DNA SMART ChIP-Seq Kit (Takara, cat# 634865). The libraries were selected from 250–350-bp fragments obtained by E-Gel 2% SizeSelect electrophoresis (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNAs were constructed using the SMARTer v4 Ultra low Input RNA kit (Takara). After cDNA shearing using a Covaris E210 instrument ...
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bioRxiv - Genomics 2020Quote: ... qPCR was performed using One Step SYBR PrimeScript RT-PCR kit (Takara Bio) on a 7900HT real-time system (Applied Biosystems).
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bioRxiv - Genomics 2020Quote: ... cDNA was synthesized using the SMART PCR cDNA Synthesis Kit (Clontech, CA, USA), and then fractionized with BluePippin® (Sage Science ...
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bioRxiv - Immunology 2021Quote: ... utilizing the PrimeScript™ One-Step RT-PCR kit (Takara Bio, Shiga, Japan). PCR products were then separated and visualized by agarose gel electrophoresis ...
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bioRxiv - Molecular Biology 2021Quote: ... In-fusion reactions were performed per the In-fusion Cloning Kit (Takara 638918) instructions and reactions were transformed into Stellar Competent Cells (Takara 636766).
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bioRxiv - Genomics 2019Quote: ... Total RNA was reverse transcribed using a PrimeScript RT-PCR kit (TaKaRa Bio), and qRT-PCR was performed using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2019Quote: ... The 5’RACE reaction was performed according to the kit manufacturer’s instructions (Clontech / Ozyme ...
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bioRxiv - Genetics 2019Quote: ... RT-PCR was accomplished using the SYBR premix Ex Taq kit (Takara, China) on an ABI StepOnePlus Real-Time PCR System (Thermofisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... cDNA was synthesized using the PrimeScriptR II 1st strand cDNA Synthesis Kit (Takara) by random primer and oligo dT primer mixture ...
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bioRxiv - Plant Biology 2019Quote: ... cDNA was obtained from 500 ng RNA using PrimeScript RT reagent Kit (Takara). Expression studies were carried out by real-time reverse transcription polymerase chain reaction (RT-qPCR ...
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bioRxiv - Cell Biology 2019Quote: ... were processed with the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech) by following manufacturer instructions ...
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bioRxiv - Immunology 2019Quote: ... RAC2 mutant 3’UTR constructs were generated using Infusion HD cloning kit (Clontech) with the following primers ...
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bioRxiv - Plant Biology 2019Quote: ... the SMARTer Ultra Low Input RNA Kit for Sequencing v4 (Clontech Laboratories, Inc.) was used to generate first strand cDNA from 50 % of the extracted RNA ...
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bioRxiv - Microbiology 2019Quote: ... Lentiviral titer was estimated using the GoStix rapid lentiviral titer detection kit (Takara Bio Europe SAS ...