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Citations for Takara Bio :
1851 - 1900 of 5307 citations for Mouse Putative Phospholipase B Like 2 PLBD2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Ribosomal RNA was removed using RiboGone – Mammalian Kit protocol (Clontech, No. 634847). After rRNA depletion ...
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bioRxiv - Developmental Biology 2023Quote: ... from cDNAs generated with SMARTer Ultra low input RNA kit (Takara Bio) according to manufacturers’ instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Reverse transcription reactions were conducted using the SMARTer RACE kit (Clontech 634859) or High-capacity cDNA Reverse Transcription kit (Applied Biosciences 4368814) ...
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bioRxiv - Cell Biology 2023Quote: ... which was reverse transcribed using the PrimerScriptTM RT reagent Kit (TaKaRa, RR037A) to obtain cDNA ...
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bioRxiv - Microbiology 2024Quote: ... placed in 1mL LBP from the NucleoSpin RNA Plus kit (Clontech #740984.250) with silica disruption beads and snap frozen in liquid nitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... which was synthesized with a PrimeScript reverse transcriptase kit (Cat# RR037A, Takara). RT–PCR was performed using a TB Green TM Premix Ex Taq Kit (Cat# RR820A ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA was synthesized using PrimeScriptTM RT reagent kit (Takara, catalog no. RR037A), and qPCR analysis was done with power SYBR Green PCR master mix (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... We conducted an In-fusion cloning reaction (In Fusion HD kit, Takara) following the manufacturer instructions to introduce the RNAi cassette into the digested intronic sequence ...
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bioRxiv - Plant Biology 2024Quote: ... and indexes from the SMARTer DNA HT Dual Index kit (Takara Bio). DNA fragments were then selected based on size using AMPure beds (Beckman Coulter) ...
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bioRxiv - Plant Biology 2024Quote: ... The libraries were prepared using the ThruPLEX DNA-seq kit (Takara Bio) and indexes from the SMARTer DNA HT Dual Index kit (Takara Bio) ...
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bioRxiv - Molecular Biology 2024Quote: ... Viruses were extracted and purified by using an AAV extraction kit (Takara).
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was made using 500ng RNA with PrimeScript RT Reagent Kit (TaKaRa). Real time PCR was set up using TB Green Premix Ex Taq II (Tli RNase H Plus ...
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bioRxiv - Cancer Biology 2024Quote: ... and reverse-transcribed using a cDNA synthesis kit (TaKaRa Bio, Kusatsu, Japan). RT-qPCR was conducted using SYBR Green PCR Master Mix (TaKaRa ...
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bioRxiv - Bioengineering 2023Quote: ... The qPCR reaction was carried out using Probe qPCR Mix kit (Takara) ...
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bioRxiv - Bioengineering 2023Quote: ... The qPCR reaction was carried out using Probe qPCR Mix kit (Takara). For each sample ...
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bioRxiv - Genomics 2024Quote: ... Dual-indexed primers (5μM) 2.0μL in Unique dual index kit (Takara bio) was used instead of 0.5 μL Index Primer in the default Zymo kit ...
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bioRxiv - Genomics 2023Quote: ... and extracted using the Nucleospin Gel and PCR Cleanup Kit (Takara, 740609) or Zymoclean Gel DNA Recovery Kit (Zymo Research ...
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bioRxiv - Biochemistry 2024Quote: ... Primers were designed using a PrimeSTAR Mutagenesis Basal Kit (Takara Bio, Inc.) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... genomic DNA was purified with Terra™ PCR Direct Genotyping Kit (Takara), followed by PCR amplification and Sanger sequencing of specific genomic regions encompassing the target site.
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bioRxiv - Cell Biology 2024Quote: ... Reverse transcription was performed using the PrimeScript RT reagent kit (Takara, Japan). The qRT-PCR was conducted using a CFX384 Real-Time PCR System (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... Total RNA was extracted using the RNAiso Plus kit (Takara, Dalian, China) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... and cDNA was synthesized by using the Reverse Transcriptase kit (RR037A, Takara). Real-Time PCR was performed by using specific primers and SYBR Green ROX Mix (RR420A ...
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bioRxiv - Microbiology 2024Quote: ... Libraries were generated using the Clontech SMARTer low input RNA kit (TAKARA) and sequenced on the Illumina NovaSeq 6000 s4 system ...
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bioRxiv - Cancer Biology 2024Quote: ... and RNA was extracted using Takara’s Nucleospin RNA Plus kit (Takara, 740984.50). RNA quantity and purity were assessed by Nanodrop 1000 ...
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bioRxiv - Microbiology 2024Quote: ... and cDNA was prepared using the Prime Script RT reagent kit (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Verification of positive colonies was achieved by co-transforming wild-type or YR-mutant TMEM39A loop domain (in pGBKT7 Vector) with genes of interest (in pGADT7 Vector) following the instruction of YeastMaker™ Yeast Transformation System 2 (Clontech, 630439) as well as plasmids from re-cloned cDNA.
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bioRxiv - Microbiology 2021Quote: ... The SARS-CoV-2 Spike sequence was cloned into the linearized pVSV-eGFP-dG by the In-Fusion cloning system (Takara Bio Inc.). The resulting pVSV-eGFP-deltaG_SARS-CoV-2 Spike vector was verified by sanger sequencing ...
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bioRxiv - Systems Biology 2019Quote: ... The relative 2 standard curves real-time PCR was carried out on the cDNA samples using TaqMan master mix (Takara, Otsu, Japan) and a Q-6000 machine (Qiagen ...
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bioRxiv - Plant Biology 2021Quote: ... SlGA2ox6 and SlGID1b-1 were constructed into pAbAi plasmid and transformed into Y1HGold yeast strain under the guidance of Yeastmaker™ Yeast Transformation System 2 (TAKARA, Japan). The recombinant yeast strain was confirmed by PCR conducted with Matchmaker™ Insert Check PCR Mix (TAKARA ...
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bioRxiv - Biochemistry 2020Quote: ... was transformed with the appropriate plasmids (see figure legends) according to the manufacturer’s instructions using the lithium acetate procedure (Clontech Matchmaker 2 manual). Liquid cultures were grown overnight in standard dropout (SD ...
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bioRxiv - Immunology 2020Quote: Transferrin over-expression or knockdown vectors were constructed and HEK 293T cells (Conservation Genetics CAS Kunming Cell Bank, China) and EcoPack™ 2–293 cells (Clontech, USA) were used to package lentiviruses and retroviruses ...
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bioRxiv - Molecular Biology 2019Quote: The qRT-PCR analysis was conducted in a total volume of 20μL containing 10μl 2 × SYBR® Premix Ex TaqII (Tli RNaseH Plus) (Takara Bio Inc., China), combined with sense and antisense primers (0.8 μl ...
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bioRxiv - Genetics 2020Quote: ... Verification of positive colonies was achieved by co-transformation of extracted bait and prey plasmids following the instruction of YeastMaker™ Yeast Transformation System 2 (Clontech, 630439) and by bait/prey plasmids with re-cloned cDNA.
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bioRxiv - Immunology 2020Quote: ... Cells were counted and seeded at 3 million cells in 1 mL of media with 2x hIL-2 into each well of a 6 well plate that was coated with 15 µg/mL of RetroNectin (Takara, Cat# T100A) for 3 hours at room temperature and subsequently washed with 1x PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR assays were performed in final volumes of 25 μl containing 12.5 μl of 2 × PCR Taq Mastermix (MgCl, dNTP, Taq enzyme) (Takara Bio Inc., Japan); 0.5μl of each primer (10 mmol/L) ...
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bioRxiv - Microbiology 2023Quote: ... The amplicons were analyzed using 2% agarose gel electrophoresis with ethidium bromide staining and using a 2000-bp DNA ladder marker (Takara, Tokyo, Japan).
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bioRxiv - Microbiology 2023Quote: ... The N-terminal 3xFLAG-tagged ORF67.5 expression plasmid was constructed using the insert extracted from a previously constructed N-terminal 2×S-tagged ORF67.5 expression plasmid digested with EcoRI and SalI (Takara Bio, Shiga, Japan) (32) ...
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bioRxiv - Microbiology 2023Quote: ... nine DNA fragments encoding the partial genome of SARS-CoV-2 (hCoV-19/Japan/TY-WK-521/2020) were amplified by PCR using PrimeSTAR GXL DNA polymerase (Takara, Cat# R050A). A linker fragment encoding the hepatitis delta virus ribozyme ...
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bioRxiv - Genomics 2023Quote: ... the human mtDNA control region (m.1-573 and m.16024-16569) was enriched using four overlapping PCR amplicons using high fidelity TaKaRa PrimeSTAR GXL DNA polymerase (TaKaRa; Table 2). PCR products were visually inspected by agarose gel ...
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bioRxiv - Biophysics 2023Quote: ... Cell debris was pelleted down and the supernatant was run on a 2 mL column volume (CV) TALON cobalt affinity resin (Takara Bio #635504) equilibrated in CoWB/TCEP (100 mM NaCl (Sigma-Aldrich 746398) ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 2 μL of cDNA product was used for subsequent RT–qPCR analysis using SYBR1 Premix Ex Taq (Takara, Dalian, Japan). All primers used in this study are shown in Table S1.
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bioRxiv - Microbiology 2023Quote: ... of the resulting fragments were subjected to a CPER reaction in a 50 μl volume using 2 μl of PrimeStar GXL DNA polymerase (Takara Bio; #R050A). The following cycling conditions were used for CPER ...
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bioRxiv - Plant Biology 2023Quote: ... genomic sequences at the length of 700∼2 000 bp were amplified from genomic DNAs with PrimeStar Max DNA polymerase (Takara, cat. R045A). The resulting fragments were cloned into vector backbones derived from pPY22 (mNeonGreen-Hygromycin B ...
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bioRxiv - Plant Biology 2023Quote: ... A volume of 2 μg of total RNA was reverse transcribed into single-stranded cDNA using AMV reverse transcriptase (TaKaRa, Dalian, China). The primer sequences used for qRT-PCR are listed in Supplementary file 1b ...
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bioRxiv - Cell Biology 2024Quote: ... A volume of 2 µl of cDNA was used as template for qPCR using SYBR Premix Ex Taq (Takara, Shiga, Japan, #RR420A). qPCR reactions were performed using an ABI 7500 system (Applied Biosystems ...
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bioRxiv - Synthetic Biology 2020Quote: ... dry milk and 0.1 % (v/v) Tween 20 and then incubated with mouse anti-6xHis tag monoclonal antibody-HRP conjugate (Clontech or Thermo Fisher Scientific, USA) in the same buffer for 2 h at room temperature ...
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bioRxiv - Evolutionary Biology 2023Quote: ... were subcloned into pCS2-3×Flag (Wang et al., 2017) or pmCherry-N1 and CDSs encoding CASP (pufferfish, human and mouse) were subcloned into p-CMV-Myc (Clontech, Mountain View, CA, USA) or pCS2-Myc (Wang et al. ...
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bioRxiv - Cell Biology 2020Quote: ... and Evl (isoform 2) were amplified by PCR from a NIH 3T3 cDNA library and ligated into suitable sites of pEGFP-C1 (Clontech, Palo Alto, CA). VASP cDNA was additionally inserted into the BglII and SalI sites of pmCherry (Addgene ID ...
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bioRxiv - Plant Biology 2020Quote: ... About 2 μg of the isolated RNA was used to synthesize the first-strand cDNA using reverse transcriptase enzyme (Takara Bio, Clontech, USA). The cDNA was diluted seven times with sterile Milli-Q water (1:7 ratio) ...
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bioRxiv - Cell Biology 2022Quote: ... Transformants were selected on synthetic media containing yeast nitrogen base (YNB, Difco) supplemented with 2 % glucose and DropOut supplements lacking uracil (Clontech, Mountain View, CA). Single colonies were used to inoculate 5 mL liquid YNB media supplemented with 2 % glucose and DropOut supplements lacking uracil ...