Labshake search
Citations for Takara Bio :
1851 - 1900 of 1941 citations for 2 Methyl 4 piperidin 1 ylsulfonyl phenylboronic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... Samples were run on SDS-PAGE and analyzed by Western blot using mouse anti-mCherry primary antibody (1:2000, Takara Bio #632543) and Licor secondary antibody (Licor #926032212 ...
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bioRxiv - Cell Biology 2021Quote: ... were gently dissociated with 0.05% Trypsin and plated onto 0.1% gelatin coated cell culture dish at a density of 1×104cells / cm2 with RHB-A medium (Clontech TaKaba Cellartis, Y40001). Medium was changed every 2 days and cultured for 4 days ...
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bioRxiv - Developmental Biology 2022Quote: c-Kit-EGFP fusion protein: chicken c-Kit cDNA was inserted into multiple cloning site of pEGFP-N1 Vector (Clontech, #6085-1) including EGFP sequences using a primer set ...
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bioRxiv - Microbiology 2021Quote: ... this results in a similar τ constant to standard settings on other instruments of 25 μF capacitance and 200 Ω resistance). Cells were immediately rescued with 1 ml of room temperature (ca. 23°C) SOC media (Takara Bio) and recovered by incubating with shaking for 1 hour at 37°C.
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bioRxiv - Immunology 2021Quote: ... viral stocks were concentrated by adding supernatant at a 3:1 ratio to Lenti-X Concentrator (631232, Takara Bio, Mountain View, CA). Mixtures were incubated overnight at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... at the concentration of 25 cells/μl in 0.5× PBS and 1× Second Diluent (640202, Takara Bio USA, Mountain View, CA, USA) into the ICELL8® 350v Chip (640019 ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 μg of total RNA was used to produce cDNA using the TaKaRa PrimeScript RT master mix (TaKaRa Bio, Otsu, Japan). Two microliters of cDNA was then used for quantitative PCR ...
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bioRxiv - Biochemistry 2020Quote: ... Polymerase chain reaction (PCR) was used to amplify the intron 1 of HIF3A using EpiTaqTM HS (for bisulfite-treated DNA; Takara, Shiga, Japan). Levels of DNA methylation were quantified using pyrosequencing with the PyroMark Q24 Advanced kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... we PCR amplified HXB2 env from the pIIIenv3-1 plasmid (NIH AIDS Reagent Program cat. #289) and cloned it into the pLVX-EF1α-IRES-Puro lentiviral vector (Clontech cat. #631988). J-Lat clone 11.1 cells were transduced with lentiviral particles produced from this plasmid and stable cell lines were generated using puromycin selection.
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bioRxiv - Biochemistry 2023Quote: ... 1 μg of total RNA was used to make cDNA libraries using prime script RT reagent kit gDNA eraser (Takara Bio Inc.) according to the kit’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: The guppy bouncer coding sequence without a stop codon was cloned from the ovary sample and inserted between the BglII and EcoRI sites of the pEGFP-N1 plasmid (Clontech, #6085-1). A nucleotide coding 2A self-cleaving peptides derived from Thosea asigna virus was artificially synthesized and added to the 3’ terminal of the bouncer coding sequence by insertion between the EcoRI and SalI sites ...
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bioRxiv - Cell Biology 2023Quote: ... KOD FX DNA polymerase (TOYOBO) and primers (supplementary table 1) and then cloned into the BamHI site of pLVSIN-EF1 α Hyg vector (Takara Bio) using an In-Fusion HD Cloning Kit (Takara Bio) ...
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bioRxiv - Neuroscience 2024Quote: ... A fragment of the mutagenized GluA2 sequence between the BstEII/BspEI restriction sites was then subcloned into the AP-SEP-GluA2 construct (prepared as described in (30)) on a pBI-Tet on vector backbone (Clontech; #6152-1). For AP-SEP-GluA2 ET/YR-BirAER ...
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bioRxiv - Pathology 2024Quote: First strand cDNA was generated from 1 μg of total RNA using the Prime Script RT Reagent Kit with gDNA Eraser (TaKaRa, Dalian, China). Real-time PCR was performed by using an SYBR Green PCR Master Mix (TaKaRa ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µg RNA was subsequently converted to cDNA using random hexamer primer using PrimeScript™ 1st strand cDNA Synthesis Kit (Takara Bio) in accordance with manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... We constructed the dlg1[4K] donor plasmid (Fig. 1) by joining five fragments into a minimal (AMP and ORI) plasmid backbone by In Fusion assembly (Takara, no. 639649). A list of primers used ...
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bioRxiv - Neuroscience 2023Quote: ... Native tdTomato fluorescence destroyed by combined ISH protocol was recovered by rabbit anti-DsRed (1:1000, Takara Bio Clontech # 632496, RRID: AB_10013483) antibody and switched to the green channel using an Alexa Fluor 488 secondary ...
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Vitamin D signaling orchestrates skeletal muscle metabolic flexibility by regulating its fuel choicebioRxiv - Physiology 2023Quote: Quadriceps muscles were dissected and snap-frozen in liquid nitrogen and homogenized in RNAiso plus Reagent (TAKARA, 1 mL/100 mg tissue) to isolate total muscle RNA as per the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2022Quote: ... and then the same amount of RNA/H2O mix (containing 1 μg of total RNA) from each sample was reverse-transcribed to cDNA using the EcoDry cDNA synthesis kit (Takara Bio, #639548) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: BON1/HA-Bcl-xL/TR-shCtBP2 and BON1/HA-Bcl-xL/TR-shRLuc #713 cells were treated with 1 µg/ml (before sorting) or 0.5 µg/ml (after sorting) doxycycline (Clontech Laboratories, Inc., 631311) for 96 hours ...
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bioRxiv - Microbiology 2024Quote: ... The rbp3 gene (slr0193) was amplified and ligated into vector pGEX-6P-1 using the In-Fusion Cloning kit (TaKaRa, Shiga, Japan) and the PCR primers pGEX-6P-1_inv-f/-r and Rbp3_iVEC-f/-r (SI Appendix ...
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bioRxiv - Cancer Biology 2024Quote: ... either AML cells were co-cultured with lentiviral particles at a seeding density of 1×106 cells per mL in a Retronectin dish (Takara Bio; T110A). HCT116 cells were seeded at a density of 0.2×106 cells per mL in a six well plate ...
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bioRxiv - Cancer Biology 2024Quote: ... a sequencing library was made using 1 ng of sheared cDNA using Low Input Library Prep Kit v2 (Takara Bio Inc, USA). DNA unique Dual index kit was used to combine libraries for sequencing (Takara Bio Inc ...
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bioRxiv - Microbiology 2024Quote: ... 1 μg extracted RNA was digested with DNase I and reverse transcribed to cDNA using a reverse transcription kit (Takara RR047A, Japan), and cDNA was used as the template for qRT-PCR analysis ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized using 1 μg of RNA from each sample using the Prime Scrip RT Master Mix Kit (TaKaRa, Dalian, China). Quantitative PCR (qPCR ...
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bioRxiv - Molecular Biology 2024Quote: Strand-specific RNA-seq was performed on parental and ibrutinib-resistant Rec-1 cells using SMARTer Stranded Total RNA Sample Prep Kit (Takara, cat# 634873) per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... AML cells and lentiviral particles were co-cultured at a seeding density of 1×106 cells per mL in a Retronectin dish (Takara Bio; T110A). Following 72 hours of infection ...
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bioRxiv - Molecular Biology 2024Quote: ... purified plasmid DNA containing either WT or mutated forms of MOP cDNA in the pTRE2hyg vector was transfected into CHO-K1 Tet-On cells (Clontech, #C3021-1) using Torpedo-DNA transfection reagent (Ibidi ...
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bioRxiv - Developmental Biology 2024Quote: ... Lentiviral particles were produced following transient transfection of the shRNA-pLKO.1 vector and packaging plasmids into Lenti-X cells (632180, Takara Bio USA) using Attractene (301005 ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 μg of total RNA was taken to synthesize cDNA by using PrimeScript 1st strand cDNA synthesis kit (Takara, Cat. No. 6110A). RT-PCR was carried out for the multiple developmental stages for amplification of zebrafish DIS3L2 with beta-actin as a control ...
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bioRxiv - Genomics 2020Quote: ... Full-length cDNA libraries were constructed from 1 μg of total RNA with SMARTer® cDNA synthesis kit (Takara Bio, Kusatsu, Shiga Japan), utilizing switching mechanism at 5’ end of RNA template (SMART ...
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The Ets protein Pointed P1 represses Asense expression in type II neuroblasts by activating TaillessbioRxiv - Developmental Biology 2021Quote: ... 1-298) (EnR) or the Ets domain of PntP1 were amplified by CloneAmp HiFi PCR Premix (Catalog# 639298, Takara Bio., Mountain View, CA) from genomic DNAs of the UAS-PntP1 line ...
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bioRxiv - Neuroscience 2020Quote: ... 16 μm in thickness) were processed for immunodetection of Mouse anti-GFP antibody (1:500, 632381, Takara Bio USA, Inc. Mountain View, CA) and incubated with secondary Donkey anti-Mouse Alexa Fluor 488 (1:100 ...
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bioRxiv - Plant Biology 2020Quote: ... First-strand cDNA was synthesized from 1 μg total RNA (20 μL reaction volume) using a PrimeScript™ 1st Strand cDNA Synthesis Kit (Takara, Dalian, China) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2020Quote: ... Quantitative PCR (qPCR) analysis of intracellular HIV-1 DNA levels was conducted using Premix Ex Taq (Probe qPCR) Rox plus (Takara Bio, Kusatsu, Japan). The oligonucleotides HIV-1 LTR and β2-microglobulin were used for HIV-1 DNA quantification and cell number determination ...
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bioRxiv - Microbiology 2020Quote: ... The PCR reaction (20 µl) contained 1.25 U of TaKaTa ExTaq Polymerase and 1 x ExTaq Buffer (Clontech Laboratories, Palo Alto, CA, USA), 312.5 µM of each dNTP ...
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bioRxiv - Cell Biology 2020Quote: ... transfected with relevant siRNA were seeded on to 8–well chamber slides and treated with 1 μM Sheild1 (632189, Clontech Laboratories UK Ltd) and 1 μM 4–hydroxytamoxifen (4–OHT ...
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bioRxiv - Cancer Biology 2022Quote: ... transduced to express CD19 CAR construct (FMC63 or CAT) with 1 ml of LV supernatant in RetroNectin®(Takara Bio, Kusatsu, Shiga, Japan)-coated 24-well plates and spinoculated at 1,000 g for 40 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Antennal cDNA was synthesized from 1 μg of antennal total RNA using SMARTer™ RACE cDNA amplification kit according to manufacturer’s instructions (Clontech, Mountain View, CA). To obtain full-length coding sequences ...
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bioRxiv - Microbiology 2022Quote: ... the sample was size fractionated using CHROMA SPIN 1000 columns to select for DNA sizes greater than 1 kb (Clontech, Mountain View, CA). After fractionation ...
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bioRxiv - Physiology 2020Quote: ... containing 1 μL of 10× reaction buffer (SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing, Cat. No. 634888, Takara Bio, Kusatsu, Japan). Nuclease-free water was added to 11.5 μL ...
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bioRxiv - Genetics 2020Quote: ... RNA was extracted from samples using TRIzol and cDNA synthesized from 1 μg RNA template using PrimeScript™ RT reagent Kit with gDNA Eraser (TAKARA BIO INC). The sequences of primers used for amplification are shown in Table S6 ...
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bioRxiv - Cancer Biology 2020Quote: ... the pEF1α-tdT/FLuc2 vector was modified to replace tdT with the fluorescence reporter zsGreen 1 (zsG) obtained from the pLVX-ZsGreen1-N1 Vector (Cat. 632565, Takara Bio USA, Inc.) and FLuc2 was replaced with the Antares2 sequence obtained from pcDNA3-Antares2 c-myc ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA (1 μg) was reverse transcribed to cDNA using the PrimeScriptTM RT reagent kit with gDNA Eraser (Cat#: RR047A, TaKaRa Bio, Shiga, Japan). qRT–PCR was performed using a LightCycler 480 II instrument (Roche Molecular Systems ...
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bioRxiv - Molecular Biology 2023Quote: ... the first-strand cDNA was synthesized from 1 μg total RNA using PrimeScript™ II 1st Strand cDNA Synthesis Kit (Takara, Beijing, China) following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... The primary antibody used was rabbit-anti-dsRed (1:500, Living Colors DsRed pAb, RRID: AB_10013483; Takara Bio USA, Mountain View, CA, USA) to enhance mCherry-labeled rVRG axon visualization ...
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bioRxiv - Cell Biology 2023Quote: ... containing a single-vector Tet-on component and were cultured in the presence of 1 µg/ml doxycycline (Clontech, Mountain View, CA, USA) during induction.
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bioRxiv - Molecular Biology 2023Quote: ... and 5 μg of total RNAs were reverse-transcribed into cDNA at 42°C for 1 h in 20 μl reaction mixture containing mouse Moloney leukemia virus reverse transcriptase (PrimeScript, TAKARA BIO, Shiga, Japan) with random 6 primers (TAKARA BIO) ...
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bioRxiv - Plant Biology 2023Quote: Genomic DNA was removed from 1 μg purified RNA using gDNA Eraser (PrimeScript RT Reagent Kit, Perfect Real Time) following manufacturer’s instructions (Takara Bio Inc., Kusatsu, Japan). Reverse transcription and cDNA was synthesized using PrimeScript Reverse Transcriptase (TaKaRa Bio) ...
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bioRxiv - Molecular Biology 2022Quote: ... Finally RNA was isolated from the eluates by phenol-chloroform-Isoamyl alcohol (25:24:1) pH 4.5 extraction and cDNA was prepared using 1st strand cDNA synthesis kit (Takara Bio Inc., Shiga, Japan) as described in above RNA analysis section ...