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Citations for Takara Bio :
1451 - 1500 of 1941 citations for 2 Methyl 4 piperidin 1 ylsulfonyl phenylboronic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: Synthetic gRNAs (1-2ng) were sequenced by SMARTer smRNA-seq kit from Takara (Cat #635030). Original oligo-dT protocol is modified and first cDNA was synthesized directly with 1µl of tailed scaffold primer (10µM ...
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bioRxiv - Cancer Biology 2024Quote: ... The two amplicons (924bp and 1149bp) were subcloned into pCMV-HA vector (Clontech, K6003-1) and sequenced ...
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bioRxiv - Bioengineering 2021Quote: ... 1 μg RNA was reverse transcribed using the PrimeScript RT reagent Kit with gDNA Eraser (Takara). The cDNA was then amplified for further analyses using deep sequencing on Illumina NextSeq platform ...
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bioRxiv - Genetics 2021Quote: ... prepare non-tissue culture treated plates by adding RetroNectin (1 μg/μL; Takara Bio, Otsu, Japan) to enhance transduction efficiency ...
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bioRxiv - Bioengineering 2021Quote: ... RNA (1 μg) was reverse transcribed using the Perfect real-time cDNA reverse transcription kit (TaKaRa). Quantitative PCR was performed with 1 μg of cDNA per sample per target gene using AceQ qPCR SYBR Green master mix (Vazyme ...
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bioRxiv - Biochemistry 2021Quote: ... 1 μg of PB-Tet-On Advanced (containing PiggyBac Transposon sequences57 flanking rtTA-Advanced (Takara Bio) and puromycin selection cassette) ...
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bioRxiv - Plant Biology 2021Quote: ... and the GFP fusion proteins were detected using the JL-8 (Clontech, 632380; 1:2500 dilution) as the primary antibody and an HRP-conjugated Affinipure Goat Anti-Mouse antibody (Proteintech ...
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bioRxiv - Plant Biology 2021Quote: ... The gRNA - dCas9 complex was amplified (primers in Supplemental Table 1) and In-Fusion cloned (Clontech) into a SacI digested pMJS064 (described above ...
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bioRxiv - Microbiology 2021Quote: ... HSV-1 strain KOS pUL21 (UniProt F8RG07) or truncations thereof was cloned into pEGFP-N1 (Clontech) for expression with a C-terminal GFP tag or into pEGFP-C2 (Clontech ...
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bioRxiv - Immunology 2021Quote: ... cDNA was synthesized from 1 μg of total RNA using an RNA reverse transcriptase kit (Takara). Real-time qPCR was performed using the ABI PRISM 7000 Sequence Detection System (Applied Biosystems) ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA (1 μg) was reversed to cDNA with PrimeScript™ RT reagent Kit (Takara, RR047Q), which was performed according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and each reaction contained 1 μl of cDNA and the SYBR Green PCR mix (Takara, RR420A), to which gene-specific forward and reverse PCR primers were added ...
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bioRxiv - Neuroscience 2021Quote: ... hLAG3 expression was then induced by addition of 1 µg/ml of Doxycycline (DOX; Clontech #631311) 3 weeks (4 week DOX on conditions ...
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bioRxiv - Neuroscience 2022Quote: ... media was collected and concentrated 1:10 in 1xPBS using Lenti-X concentrator (Takara Bio, #631231), aliquoted ...
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bioRxiv - Developmental Biology 2020Quote: ... dissected calvaria from 9SL or 11SL fish were labeled with antibodies against mCherry (Takara, 1/100), GFP (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was prepared with 1 µg RNA and synthesized using the Primescript RT kit (Takara Inc.). qPCR assays were performed on the StepOnePlusTM Real-time PCR System (Applied Biosystems ...
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bioRxiv - Cell Biology 2020Quote: ... gDNA of CFU colonies was extracted by suspending the cell in 1×PCR buffer (Takara, R050A) containing 0.2mg/ml proteinase K (Tiangen ...
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bioRxiv - Immunology 2020Quote: ... EqPD-1 and EqPD-L1 cDNAs were amplified by PCR using TaKaRa Ex Taq (Takara Bio) and specific primers (Supplementary Table 1) ...
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bioRxiv - Neuroscience 2023Quote: ... Antibodies and dyes were used at following dilutions: rabbit-α-dsRed (1:500, Takara, #632496; RRID:AB_10013483), α-HRP conjugated with Alexa Fluor-488 (1:250 ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were added to the blocking solution (Rabbit anti-DsRed 1:1000, stock #632496, Takara Bio ...
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bioRxiv - Bioengineering 2023Quote: ... Cell and tissue were stained with the following primary antibody: rabbit anti-ZsGreen (1:500; Takara), rabbit anti-NeuN (1:500 ...
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bioRxiv - Molecular Biology 2023Quote: ... EGFP-SEC61B was generated by cloning the corresponding cDNA into the pEGFP.C1 vector (Clontech, 6084-1). The plasmid pRK5-HA-UB was described previously [56] ...
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bioRxiv - Physiology 2023Quote: ... Slides were incubated for 18 hours at room temperature in anti-dsRed antibody (TaKaRa, 1:2000) in 0.1M PB containing 0.3% Triton X-100 and 1% donkey serum ...
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bioRxiv - Biochemistry 2023Quote: ... the expression of GADD34Δ and K3L was induced by adding 1 µg/ml of doxycycline (Takara). After one additional day ...
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bioRxiv - Cell Biology 2023Quote: ... His-tagged recombinant proteins were isolated by 1 h incubation with Talon metal affinity resin (Clontech) at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... For immunostaining the slides were incubated with primary antibodies anti-DsRed (Takara Bio, #632496, 1/1000), anti-Pax7 (purified hybridoma ...
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bioRxiv - Cell Biology 2023Quote: ... Immunoblotting was performed using an anti-GFP mouse monoclonal antibody (JL8; 1:1000; TaKaRa Bio Inc.) and an anti-ubiquitin mouse monoclonal antibody (P4D1 ...
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bioRxiv - Neuroscience 2022Quote: ... then incubated overnight with the primary antibody rabbit anti-dsRed (1:1000 in PBST; Clontech, AB_10013483) at 4°C ...
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Constitutive and conditional epitope-tagging of endogenous G protein coupled receptors in DrosophilabioRxiv - Neuroscience 2023Quote: Myristoylated TdTomato (myr::TdTom) was labeled with 1:500 rabbit anti-DsRed (Takara Cat# 632496, RRID:AB_10013483)
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bioRxiv - Neuroscience 2024Quote: ... The following primary antibodies were used for staining: anti-FOXG1 (rabbit, Takara, M227, 1:400 dilution), anti-TCF7L2 (rabbit ...
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bioRxiv - Cell Biology 2023Quote: Procollagen Type 1 C-peptide (PIP) was measured according to the manufacturer’s instructions (Takara, Shiga, Japan). For evaluation of PIP ...
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bioRxiv - Neuroscience 2023Quote: ... a mix of an mCherry-DsRed rabbit polyclonal antibody (Living Colors-Clontech Antibody 632496, 1:1000) and a mouse monoclonal antibody against oxytocin (P38 ...
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bioRxiv - Plant Biology 2023Quote: ... 1 μg total RNA was reverse transcribed into cDNA with the SMARTer RACE 5’ Kit (Clontech). PCR was performed using primer GSP-HO1 and the Universal Primer Mix (UPM ...
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bioRxiv - Plant Biology 2024Quote: ... Both PHI-1 ORF and CESAs ORFs were amplified from first-strand DNA with PrimeScriptRTase (TaKaRa) using primers indicated in Supplemental information 4 ...
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bioRxiv - Plant Biology 2024Quote: ... 1 μg of total RNA was reverse transcribed using SMART® MMLV Reverse Transcriptase (Clontech, www.clontech.com) using oligo(dT)15 primers ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was directly loaded into a gravity column filled with 1 mL TALON resin (TaKaRa) pre-equilibrated with the binding buffer (20 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... Input and elution samples were analyzed by immunoblot using mouse anti-GFP (1:5,000, Clontech #632381) to detect A3-EGFP ...
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bioRxiv - Genetics 2022Quote: Full length wild-type or mutant BRC-1 sequences were cloned into plasmid pBridge (Takara Bio), transformed into yeast strain Y2HGold (Takara Bio ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 ng of total RNA was reverse transcribed with the cDNA synthesis kit (Takara Bio Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: Purified proteins or the artificial motor-cargo complex were diluted using 1× PBS (Takara Bio, T900) and loaded into custom-made microneedles prepared with the P1000IVF micropipette puller (Sutter Instrument) ...
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bioRxiv - Neuroscience 2024Quote: The primary antibodies used were: rabbit anti-dsRed (Living Colors® Clontech, used at 1/1000), rabbit anti-Vangl2 58 ...
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bioRxiv - Pathology 2024Quote: ... cDNA was synthesized from 1 µg of RNA with a PrimeScript RT reagent Kit (Takara, RR037A). Pluripotency genes were amplified with gene-specific primers by PCR using Taq DNA polymerase (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized from 1 µg of total RNA using the Primescript RT Master Mix (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The following primary antibodies were used for staining: anti-FOXG1 (rabbit, Takara, M227, 1:400 dilution), anti-TCF7L2 (rabbit ...
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bioRxiv - Cell Biology 2024Quote: ... 1 µg of total RNA was reverse transcribed to cDNA using SMART MMLV reverse transcriptase (Takara) and Oligo(dT)20 as a primer ...
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bioRxiv - Plant Biology 2024Quote: ... was amplified from Tak-1 genomic DNA by PCR using PrimeSTAR Max (Takara Bio Inc., Japan) with the primers IF-MpRLFpro-F and IF-MpRLFpro-R ...
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bioRxiv - Plant Biology 2024Quote: ... we use 1 μg of purified total RNA using the PrimescriptTM RT master mix (Takara, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 ng RNA was reverse-transcribed using the SMART-Seq v4 Low Input RNA kit (Takara). cDNA was quantified and qualified using Qubit (Invitrogen ...
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bioRxiv - Biophysics 2021Quote: ... which was digested with XhoI and BamHI restriction enzymes from the EYFP-actin vector (#6902-1, Clontech) into the mClover2-C1 vector (Addgene plasmid #54577 ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA synthesized from 1 μg total RNA using PrimeScript™ RT reagent Kit with gDNA Eraser (Takara). Real-time PCR was performed using TB Green® Premix Ex Taq™ II (Tli RNaseH Plus ...