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Citations for Takara Bio :
1551 - 1600 of 1852 citations for 1 Piperidin 4 yl azetidine 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... 10 µl of RNA extraction mixture was then added to 1 µl of 10 mM dNTPs (Takara Catalog # 4030) and 100 mM of oligo-dT(18 ...
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bioRxiv - Plant Biology 2023Quote: ... The full-length and truncate CDS of TaNAM-A1a (residues 1–220) were cloned into bait vector pGBKT7 (Clontech) respectively to generate bait plasmids BD-NAM-A1full and BD-NAM-A11-220 ...
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bioRxiv - Molecular Biology 2023Quote: ... Equal loading of the GFP tagged E2F1 proteins was determined by the GFP antibody (Clontech, lot. 1404005; 1:2000) the secondary antibody anti-rabbit HRP (Na934v ...
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bioRxiv - Plant Biology 2024Quote: ... First-strand cDNA was synthesized from 1 µg of purified total RNA using PrimeScript RT Master Mix (TaKara, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... 0.5-1.0 μg total RNA was used for cDNA synthesis with 5x PrimeScript RT Master Mix (Takara, RR036A-1). Quantitative real-time PCR was carried out using PowerUp SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Biophysics 2024Quote: ... the solution of carboxylated beads was diluted into 400 µL of 1× Phosphate-Buffered Saline (PBS) (Takara Bio, T900) containing 100 mg/mL PLL-g-PEG (SuSoS ...
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bioRxiv - Immunology 2024Quote: ... 1 µg of total RNA was reverse transcribed using the PrimeScript 1st strand cDNA Synthesis kit (Takara, catalog 6110A). The resulting cDNA was employed for qRT-PCR using PowerUp SYBR Green Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... Transformed yeast cells were grown under selective conditions in a minimal medium (46.7 g L-1 Minimal SD Agar Base [TaKaRa] ...
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bioRxiv - Biochemistry 2024Quote: ... Induction of the constructs was done by supplementing the medium with 0.5 µg ml-1 tetracycline (Tet) (Takara Bio).
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bioRxiv - Neuroscience 2024Quote: ... Cells were collected in a lysis buffer (0.3% NP-40, 0.1% FBS, 1% RNase Inhibitor) and converted into cDNA directly (Takara, #RR036A). qRT-PCR was performed with 200cells per well and three technical triplicates using the following gene-specific primers:
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bioRxiv - Developmental Biology 2024Quote: ... resuspended in storage buffer (1% BSA in PBS, with 10% DMSO and 1U/µl recombinant RNAse inhibitor, Takara Bio) and stored at −20° C for further processing.
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bioRxiv - Plant Biology 2024Quote: ... Immunoreactive signals were detected using an ECL detection system (GR Healthcare) with anti-GFP antibody (1:5,000; JL-8; Clontech), anti-actin antibody (1:2,000 ...
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bioRxiv - Synthetic Biology 2024Quote: ... followed by incubation at 37°C for 1 hour with 2 µl of Klenow Fragment (Takara Bio Inc, Japan) and inactivation at 65°C for 5 minutes ...
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bioRxiv - Neuroscience 2024Quote: Fluorescent immunohistochemistry was performed on free-floating coronal sections from each animal using antibodies against zsGreen (1:1000, Clontech), STEM121 (1:500 ...
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bioRxiv - Plant Biology 2020Quote: ... An additional BbsI site in the pDONR backbone was eliminated by site-directed mutagenesis using primers noBbsI_F and noBbsI_R (Supplemental Table 1) followed by an In-Fusion reaction (Takara Bio USA). In order to yield the final binary vectors ...
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bioRxiv - Microbiology 2020Quote: ... CTCF BS1 and CTCF BS2 were mutated by site-directed PCR mutagenesis using the primers detailed in Table 1 and Prime Star Max (Takara) mutagenesis kit following the manufacturer’s protocols and confirmed by sequencing ...
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bioRxiv - Developmental Biology 2021Quote: Cells were lysed and RNA was reverse transcribed and amplified according to the SMARTer Ultra Low RNA Kit for Illumina Sequencing (version 1, Clontech). Libraries were prepared from cDNA using the NEBNext Ultra DNA library preparation for Illumina sequencing with indexed adaptors (New England Biolabs) ...
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bioRxiv - Developmental Biology 2021Quote: Two-hybrid screening and assays were performed as described in the Matchmaker™ Pretransformed Libraries User Manual (Takara PT3183-1) using a Drosophila Mate & Plate™ Library - Universal Drosophila (Normalized A+ RNA isolated from embryo (~20 hr) ...
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bioRxiv - Genetics 2021Quote: ... Table 1) against two unique sequences in the spCas9 that differs from dCas9GCN4 using Xfect RNA transfection reagent (Takara #631450) according to manufacturer instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... DNA libraries were prepared using 1-5 ng of starting material using the SMARTer ThruPLEX DNA-Seq kit (Takara; R400674) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were diluted 30 or 60 times in 100 µl mTeSR with 1:1000 Y-27632 (STEMCELL, 72302) and 2µg/ml doxycycline (Clontech, 631311) and the cell suspension was added to a well containing the transfection complexes ...
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bioRxiv - Developmental Biology 2021Quote: The cDNAs for HA-tagged ELF3 were amplified from the KhES-1 cDNA library by PCR using PrimeSTAR GXL (Takara) and were subcloned into the pENTR/D entry vector (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... and first-strand cDNA was synthesized with 1 μg of total RNA using a SuperScript III First-Strand Synthesis System for RT-PCR (TaKaRa) following the manufacturer’s protocol.
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bioRxiv - Plant Biology 2022Quote: ... these plasmids were co-transformed into the Y2H GOLD strain in an activation domain (AD)-BD coupled manner (Yeast Protocols Handbook, PT3024-1; Clontech). The primers used are listed in Supplemental Table S1.
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bioRxiv - Neuroscience 2021Quote: ... Groups of 6-10 cells were dispensed from the micropipette into 1 µl ice-cold 10x reaction buffer (SMART-Seq v4 kit, Takara) and flash-frozen before library preparation ...
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miR-125-chinmo pathway regulates dietary restriction dependent enhancement of lifespan in DrosophilabioRxiv - Molecular Biology 2020Quote: ... The synthesized cDNA was diluted (1:10) and used as template for quantitative real-time PCR (qRT-PCR) using SYBR premix EX-Taq-plus (TaKara) and analyzed on QuantStudio 6 Real-Time PCR machine (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... The viral media was filtered through 0.45 μm filter followed by concentration through Amicon column and addition of 1/3rd volume of LentiX Concentrator (Takara #631231). Viral pellet was obtained by spin down at 1500g at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... sperm from 15 flies were dissected and pooled in 1:10 dilution of RNAse inhibitor (Recombinant ribonuclease inhibitor 5 000 U, Cat. 2313A Takara), and samples were flash-frozen on dry-ice ...
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bioRxiv - Neuroscience 2021Quote: ... 1% normal donkey serum) for 1h at room temperature followed by incubation in primary antibodies (rabbit anti Ds-red, Takara, cat# 632496 ...
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bioRxiv - Physiology 2021Quote: ... Brain sections were then incubated overnight at room temperature in blocking solution containing primary antiserum (rat anti-mCherry, Life Technologies M11217, 1:1,000; rabbit anti-dsRed, Clontech 632496 ...
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bioRxiv - Cancer Biology 2020Quote: ... Total RNAs of 0.5 to 1 μg were used as templates for the reverse transcription using PrimeScriptTM RT Reagent Kit with gDNA Eraser (Takara). Quantitative PCR (qPCR ...
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bioRxiv - Cell Biology 2021Quote: ... pLL5.0-EGFP-HRasC20 was generated by insertion of EGFP-HRasC20 fragment which was amplified from pEGFP-F (#6074-1; Clontech) by PCR into pLL5.0 vector ...
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bioRxiv - Neuroscience 2021Quote: ... Virus was then concentrated from the media 1:10 in PBS using Lenti-X concentrator (Takara Bio, Cat. No. 631231), aliquoted and stored at −80°C for future use.
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bioRxiv - Microbiology 2020Quote: ... Double stranded cDNA was prepared from 1 μg of each mRNA sample using the PrimeScript Double Strand cDNA Synthesis Kit (Takara) and a cDNA library was established with Ligation Sequencing Kit (SQK-LSK109 ...
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bioRxiv - Neuroscience 2020Quote: ... and first-strand cDNA was synthesized from 1 ⎧g of total RNA by oligo(dT) priming using SMARTScribe Reverse Transcriptase (Clontech) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2021Quote: ... The RNA (1 ug/sample) was reverse transcribed into cDNA and Q-PCR was performed using a SYBR Green PCR kit (Takara) in a Light Cycler (Eppendorf) ...
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bioRxiv - Systems Biology 2021Quote: ... cDNA was synthesized from 1 µg of total RNA using the PrimeScript RT reagent kit (Perfect Real Time) (cat. no. RR037A, TaKaRa) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... following patch-clamp cells were collected using a separate wide-bore collection pipette (0.2-0.5 MOhm) filled with lysis buffer (10% Triton, ribonuclease inhibitor 1:40; Clontech, Cat#2313A), ERCC RNA spike-in mix (1:600000 ...
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bioRxiv - Cell Biology 2020Quote: ... GFP CIZ1 C275 was made by ligating the 1 kb C-terminal XhoI fragment (Coverley et al., 2005) into the XhoI site of pEGFP-C2 (Clontech). GFP CIZ1 Δp8 (this study ...
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bioRxiv - Cancer Biology 2020Quote: ... a targeting vector was constructed (Supplementary Figure 1) to include a heptameric tetracycline-response element (TRE) derived from the pTRE2 vector (Clontech), inserted into the intron 2 of the endogenous Myc locus that serves as a binding site for tetracycline-regulatable transcriptional repressors ...
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bioRxiv - Molecular Biology 2022Quote: ... gDNA extracted and PCR genotyped with primers (Supplemental table 1) flanking each homologous arms using PrimeStar GXL DNA polymerase (Takara). Correctly targeted clones were further expanded and banked ...
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bioRxiv - Microbiology 2022Quote: ... and A549-MxB(1-715)-MxB(26-715) cells expressing untagged MxB(1-715) and MxB(26-715) using the pLKOD-Ires-Puro vector (Clontech Takara Bio ...
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bioRxiv - Neuroscience 2022Quote: ... Incubation with primary antibodies was performed in blocking buffer containing rabbit anti-DsRed (1:1000, 632496; Clonetech-Takara Bio, Japan) antibody at 4°C for 1–2 days ...
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bioRxiv - Cancer Biology 2022Quote: ... cDNAs were diluted 1/10 and PCR reactions were conducted using SYBR qPCR Premix Ex Taq II Tli RNase H+ (TAKRR820W, Takara). Primers can be found in Supplementary Table 4 ...
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KIF24 controls the clustering of supernumerary centrosomes in pancreatic ductal adenocarcinoma cellsbioRxiv - Cell Biology 2022Quote: ... human KIF24 fragments encoding residue 1-4383 was excised from pEGFP-C1-KIF24 (Kobayashi et al., 2011) and sub-cloned into pLVX-IRES-Puro (Clontech). KIF24/TS621-622AA (TS ...
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bioRxiv - Neuroscience 2021Quote: ... We washed nuclei once with EZ PREP buffer and once with Nuclei Suspension Buffer (NSB; consisting of 1× PBS, 0.01% BSA and 0.1% RNase inhibitor (Clontech, Cat# 2313A)) ...
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bioRxiv - Neuroscience 2020Quote: ... cultures were infected with CC1-mycDD-expressing lentivirus at DIV4 and treated on DIV7 with 1 μM Shld1 (Takara Bio) or vehicle (0.2% (v/v ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative real-time PCR reactions were performed using 1/60 of the immunoprecipitated DNA and the TB Green Premix Ex Taq II (Takara). Relative quantification using standard curve method was performed for each primer pair and 96-well Optical Reaction plates were read in a StepOnePlus PCR instrument (Applied Biosystem) ...
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bioRxiv - Molecular Biology 2020Quote: Primary RT-PCR and secondary PCR was performed using the Primescript III 1-step RT-PCR kit and EmeraldAmp GT PCR Mix (Takara; detailed protocol provided in Supplementary File 1) ...
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bioRxiv - Microbiology 2021Quote: ... PCR amplifications were conducted in 50-μL total volumes containing 1 μL of template DNA using an ExTaq kit (Takara). The cycling protocol was as follows ...