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Citations for Takara Bio :
1501 - 1550 of 5211 citations for Ceruloplasmin Colorimetric Activity Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... The lysate was loaded onto a prepared column with 2 mL TALON Metal Affinity Resin (Clontech Laboratories, Inc., Mountain View, CA). After being washed twice with 5 column volumes (CV ...
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bioRxiv - Systems Biology 2020Quote: ... proteins in whole cell extract and immunoprecipitated fractions were digested with 2 mg/mL proteinase K (Takara Bio Inc., Kusatsu, Japan) at 42°C for 2 h ...
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bioRxiv - Cell Biology 2021Quote: γ2 mCherry and tethered GluA2 (flop isoform)::γ260 were subcloned into the doxycycline-inducible expression vector pBI-Tet (Clontech, #6152-1) using the restriction sites MluI/XbaI and MluI/NheI ...
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bioRxiv - Cancer Biology 2021Quote: We quantified mRNA expression levels of IL-10 and chemokines identified in the RNA-Seq analysis using 2-step real-time RT-PCR (Thermal Cycler Dice Real Time System, Takara Bio). The ribosomal protein L13a (RPL13A ...
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bioRxiv - Cancer Biology 2021Quote: ... whereas a TOPBP1 fragment corresponding to residues 2-1523 was amplified from pCDNA5-FRT/TO-LacR-FLAG-TopBP1 and cloned into pGBKT7 (Clontech/Takara) to create a fusion with the GAL4 DNA binding domain using the NdeI and XmaI sites ...
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bioRxiv - Neuroscience 2022Quote: ... protein lysate was immunoprecipitated for 2 h at 4°C with the following antibodies: mouse anti-GFP (1:1000; Takara Bio), mouse anti-FLAG (1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... Transferred RNA was UV-crosslinked to membrane and hybridized with [γ- 32P] 5’-end labeled RFP-spacer specific probe (RFP_crRNA_probe, Supplementary Table 2) in ExpressHyb solution (Clontech Laboratories, Inc) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... 1 μl sense and 1 μl anti-sense primers of 100 μM each were mixed with 2 μl 10X Taq polymerase PCR buffer (Takara, Japan) and 16 μl ultra-pure water to a final volume of 20 μl ...
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bioRxiv - Molecular Biology 2019Quote: ... with a universally tailed poly-dT primer and a template switching oligo followed by amplification for 12 cycles with the Advantage 2 DNA Polymerase (Takara Bio). After ultrasonic shearing (Covaris LE220) ...
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bioRxiv - Microbiology 2021Quote: ... real-time RT-PCR was performed with SARS-CoV-2 N primer sets and SYBR Premix Ex Taq II (TaKaRa-Bio) using a LightCycler Nano (Roche ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR reactions were performed in a 25μl-reaction mixture containing 12.5μl of 2 × Gflex PCR Buffer (Mg2+, dNTP plus) (TaKaRa Bio Inc., Shiga, Japan), 0.5μl of Tks
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bioRxiv - Physiology 2021Quote: ... Frozen placental biopsies (25mg) were homogenized using a 2 ml Kimble dounce tissue grinder and nuclei pellets resuspended in Nuclei Suspension Buffer (Clontech #2313A) before filtering through a 40μm cell strainer ...
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bioRxiv - Cell Biology 2020Quote: ... foetal bovine serum or 10% (v/v) TET System–approved FCS for U–2–OS reporter cell lines (631106, Takara Bio). U–2–OS-pEP15 cells (5 ...
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bioRxiv - Molecular Biology 2020Quote: Yeast-2-hybrid assays were performed with the plasmids and strains from the Matchmaker Gold Yeast Two-Hybrid System (Takara Bio) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... The amplified PCR product was verified by sequencing and then cloned into modified XW55 yeast dual expression vector65 in the highlighted SpeI and PmlI sites downstream of the ADH2 promoter by yeast recombinase technology [protocolYeastmaker™ YeastTransformation System 2 (Clontech)] and according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... the transfection medium was replaced with 2 ml of reduced serum culture medium (5% FCS) supplemented with 300 μM A/C heterodimerization agent (formerly AP21967, Takara BioInc), 20 mM HEPES and 10 μM cholesterol (balanced with methyl-β-cyclodextrin ...
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bioRxiv - Molecular Biology 2022Quote: ... serving as homology arms for directional cloning of the library into RD-seq plasmid (zfh1-DSCP-Gal4-DBD, Suppl. Table 2) vector using In-Fusion HD (Clontech 639650).
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bioRxiv - Genomics 2022Quote: The genomic DNA of Mir-0 accession (ID 8337) was used as a template (2 ng) for amplification using PrimeSTAR® GXL DNA Polymerase (TaKaRa), in a 40-µl PCR reaction with 0.3 µM primers OP009 and OP010 ...
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bioRxiv - Microbiology 2022Quote: ... These fragments were cloned into pUC19 with the HYG resistance cassette upstream of pCTR4-2 with InFusion cloning (Takara, Cat#638911). Plasmids were isolated from individual clones and confirmed by digestion with HindIII (New England Biolabs ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... in a 20 µL reaction consisting of 10 μL of TB Green Premix Ex Taq 2 (TliRNaseH Plus; TaKaRa, Dalian, China), 1 µL each of forward and reverse primer ...
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bioRxiv - Plant Biology 2022Quote: ... Each of the up-regulated genes (MpGSTF7, MpGSTF10, MpGSTF15, MpCYP813A5, MpCYP822A1) was PCR amplified from genomic Tak-2 DNA using CloneAmp HiFi Premix (Takara Bio). The primers used to amplify gene sequences are listed below ...
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bioRxiv - Biochemistry 2022Quote: ... KM881710.2) were PCR amplified from viral cDNA templates (31–33) and cloned into pSUMO expression plasmid by IN-FUSION (Takara Bio). The final constructs were confirmed by sanger sequencing performed by Genewiz.
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bioRxiv - Immunology 2023Quote: ... Lentivirus-containing supernatant was harvested on days 2 and 3 after transfection and then concentrated using a Lenti-X concentrator (Clontech, 631232). HEK-293T cells were transfected with the expression vectors according to the manufacturer’s protocol with PEI 2500 (BioScientific) ...
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bioRxiv - Biochemistry 2022Quote: ... cDNAs were generated by PCR amplification in 50 µl-total reaction volume using the Advantage 2 PCR Enzyme System (Clontech, #639206). PCR reaction was performed using 20 µl cDNA from previous step ...
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bioRxiv - Immunology 2023Quote: ... The mixture was then chilled on ice for 2 min and then mixed with 4 μl 5x first-strand buffer (Takara Bio), 2 μl 100 mM DTT ...
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bioRxiv - Synthetic Biology 2023Quote: ... T cells were incubated at 3×104 cells/well in 96-well U bottom plates for 24 hours in T cell media containing 50 IU/mL rhIL-2 and varying concentrations of AP20187 (B/B homodimerizer, Takara, 635058). After 24 hours ...
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bioRxiv - Plant Biology 2024Quote: ... were enriched from the lysate by immunoprecipitation using GFP-Trap (Lablead, GNM-25-1000) and were partially digested by micrococcal nuclease (2 × 10−5 U/μL, Takara, 2910A). The digested RNA was ligated to the 3′-RNA adaptor labeled by biotin ...
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bioRxiv - Molecular Biology 2024Quote: ... the U2OS 2-6-3 cells expressing degron-tagged LacI fusion proteins were incubated with 300 nM Shield1 ligand (Takara Bio) and 1 µg/mL doxycycline for 24 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... Reverse transcription was done using the PrimeScript™ RT reagent Kit (Takara) according to the manufacturer’s recommendation ...
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bioRxiv - Microbiology 2020Quote: ... except ligation was performed using a BD In-FusionTM cloning kit (Clontech), according to the manufacturer’s instructions (primers listed in SI Appendix Table S12) ...
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bioRxiv - Molecular Biology 2020Quote: ... pCLT-mt1 and mt2 were generated by PrimeSTAR Mutagenesis Basal Kit (Takara). pET22b-TruB1 ...
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bioRxiv - Cell Biology 2020Quote: Purified RNA was transcribed into cDNA using the PrimeScript Reagent Kit (Takara). The volume equivalent of 0.2 µg of RNA was reverse transcribed in a 40 µL reaction volume ...
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bioRxiv - Genetics 2021Quote: ... and reverse transcription was performed using the PrimeScript RT Reagent Kit (Takara). Equal masses (concentration times volume ...
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bioRxiv - Genetics 2021Quote: ... Libraries were prepared using SMARTer ThruPLEX DNA-Seq Kit (Takara Bio # R400675)
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bioRxiv - Genetics 2021Quote: Using the SYBR Premix Ex TaqTM II kit (Takara Biotechnology, Tokyo, Japan) to perform the quantitative real-time PCR assay with CFX ConnectTM Real-Time PCR Detection System (Bio-Rad Laboratories ...
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bioRxiv - Genetics 2020Quote: ... was generated with the In-Fusion HD Cloning Kit (Clontech Laboratories, Inc.). The pCFD3-dU63gRNA plasmid ...
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bioRxiv - Microbiology 2021Quote: ... it was reverse transcribed using the PrimeScript™ RT reagent Kit (TaKaRa). The GPC segment was amplified and sequenced using primers ...
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bioRxiv - Neuroscience 2021Quote: ... The amplified fragment was ligated using the In-Fusion cloning kit (Takara) into the pGL4.13[luc2/SV40] (E6681 ...
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bioRxiv - Developmental Biology 2021Quote: ... The RNA was reverse-transcribed with PrimeScript™ RT reagent Kit (Takara) and RT-PCR was analyzed with gene specific primers listed in Table S1 ...
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bioRxiv - Plant Biology 2022Quote: All constructs were obtained with the In-Fusion HD cloning kit (Takara) and sequence verified ...
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bioRxiv - Molecular Biology 2022Quote: 5’ RACE was performed using SMARTer RACE 5’/3’ Kit (TAKARA #634858) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... RT-qPCR was performed using a First Strand cDNA Synthesis Kit (TaKaRa), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.4 μL of 25 mM Dntp (Advantage UltraPure dNTP Combination Kit) (TaKaRa), 4.0 μL of 5× SSIV Buffer (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... qRT-qPCR was performed with a SYBR PrimeScript RT-qPCR Kit (Takara) for 40 cycles at 95°C for 5 s and 60°C for 30 s on a Light Cycler 480 Real-Time PCR System (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... Viruses were titered using the Lenti-X p24 rapid titer kit (Takara). SH-SY5Y were transduced in 96-well plates at multiplicity of infection (MOI ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was generated with a PrimeScript RT Kit (Takara, Otsu, Shiga, Japan). Q-PCR was conducted to detect RNA amounts using FastStart Universal SYBR Green Master (ROX ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were purified using Capturem™ His-Tagged Purification kit (Takara Bio), then dialyzed by PBS buffer overnight ...
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bioRxiv - Immunology 2021Quote: ... LDH release was quantified using an LDH Cytotoxicity Detection Kit (Takara BioProduct) according to the manufacturer’s instructions and normalized to mock-infected cells.
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bioRxiv - Genomics 2020Quote: ... and RNA-seq libraries generated using the SMARTer cDNA synthesis kit (Takara), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Quantitative PCR was carried out using SYBR Green Q-PCR kit (Takara).