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Citations for Takara Bio :
101 - 150 of 552 citations for Protein A G Magnetic Beads for IP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... PCR products were cleaned with NucleoMag beads (Takara Bio, Catalog #744503) and then visualized using gel electrophoresis ...
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bioRxiv - Cell Biology 2024Quote: ... Supernatants were loaded on 5 mL of TALON beads (Takara Bio) pre-equilibrated with the lysis buffer ...
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bioRxiv - Biochemistry 2019Quote: ... Protein purity and concentration were determined by SDS-PAGE and BCA protein assay kit (Takara) respectively ...
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bioRxiv - Biophysics 2019Quote: ... cDNAs encoding cyan fluorescent protein (CFP) and yellow fluorescent protein (YFP) were purchased from Clontech Com ...
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bioRxiv - Cell Biology 2022Quote: ... Total protein was quantified using the TAKARA BCA Protein Assay Kit (TAKARA Bio Inc., Japan). Equal amounts of protein were separated by SDS-PAGE on 10% gels ...
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bioRxiv - Immunology 2021Quote: ... and the protein concentration was measured by BCA protein assay kit (TaKaRa, Dalian, China, cat#T9300A) as previous described(Ma et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein concentration was found to be 0.9 µg/µl using BCA Protein Assay Kit (TaKaRa).
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bioRxiv - Microbiology 2022Quote: Protein-protein interactions were assayed with the Matchmaker yeast two-hybrid system (Clontech, Mountain View, CA). ORFs of AoHse was amplified from first-strand cDNA of A ...
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bioRxiv - Physiology 2022Quote: ... Obtained luminescence was normalized to total protein concentration measured by BCA protein assay kit (Takara Bio).
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bioRxiv - Microbiology 2024Quote: Protein concentrations were determined using the BCA method (TaKaRa BCA Protein Assay Kit; Takara, Shiga, Japan) after 1% SDS was added to solubilize the samples ...
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bioRxiv - Neuroscience 2020Quote: ... Flow cytometry calibration beads for AcGFP1/eGFP and mCherry (Takara, 632594, 632595) were used as fluorescence intensity controls ...
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bioRxiv - Neuroscience 2021Quote: ... Cell extract is filtered and incubated with washed TALON beads (Takara 635501) at 4C for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech). Fractions by imidazole-elution were subjected to Hi-trap SP cation-exchange chromatography ...
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bioRxiv - Cell Biology 2020Quote: ... and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech). Fractions by imidazole-elution were subjected to Hi-trap SP cation-exchange chromatography ...
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bioRxiv - Cell Biology 2019Quote: ... and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech). Fractions by imidazole-elution were subjected to Hi-trap SP cation-exchange chromatography ...
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bioRxiv - Cell Biology 2019Quote: ... and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech). Fractions by imidazole-elution were subjected to Hi-trap SP cation-exchange chromatography ...
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bioRxiv - Microbiology 2023Quote: ... Clarified lysates were incubated with TALON Metal Affinity Resin cobalt beads (Takara), and beads were then washed with Tris-HCL (pH 8.0 ...
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bioRxiv - Neuroscience 2020Quote: Enhanced green fluorescent protein (EGFP, Clontech), Tag-blue fluorescent protein (Tag-BFP ...
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bioRxiv - Molecular Biology 2022Quote: ... protein was estimated using BCA (TaKaRa), and 100 µg protein was incubated with 20 µl packed protein G-sepharose beads (Sigma P3296 ...
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bioRxiv - Zoology 2020Quote: ... The protein concentration was measured by TaKaRa BCA Protein Assay Kit (Takara Bio) in accordance with the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... 5) cDNA coding eGFP protein (Clontech). 6 ...
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bioRxiv - Biochemistry 2021Quote: ... an aliquot of the lysate was used to measure protein concentration by BCA protein assay (Takara Bio). The lysate was mixed with Optiphase Hisafe 3 (PerkinElmer) ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentrations of cell lysates were measured using a bicinchoninic acid protein assay kit (Takara Bio Inc.). Cell lysate containing the same amount of protein was mixed with 10 volumes of methanol and then centrifuged at 20,000 × g for 30 min to precipitate proteins ...
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bioRxiv - Molecular Biology 2021Quote: ... Lentiviral supernatants were produced using Lenti-X Packaging Single Shots (VSV-G) (Clontech #631275) mixed with pLKO.1 puro-based lentiviral constructs transfected to Lenti-X 293T cells ...
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bioRxiv - Cell Biology 2020Quote: ... 4 °C for 45 min and the supernatants incubated with TALON beads (Clontech) pre-equlibrated with purification buffer at RT over night with overhead rotation followed by washing with wash buffer (8M urea ...
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bioRxiv - Genomics 2022Quote: ... cleaned up with NucleoMag NGS Clean-up and Size Select beads (Takara Bio), and ligated with the aforementioned triple-digested plasmid pool using T4 DNA ligase (New England Biolabs ...
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bioRxiv - Plant Biology 2020Quote: ... The remaining RT primers were digested on the beads with Exonuclease I (TAKARA) (37°C for 30 min ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was incubated with α-cMyc agarose beads (Takara Bio, Kyoto, Japan) on a rotary wheel for 16 h at 4 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... The bead suspension was treated with 0.25 unit/µl Micrococcal Nuclease (Takara Bio) at 25°C for 15 min ...
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bioRxiv - Biochemistry 2024Quote: ... After addition of DNaseI 1mM MgCl2 and 200μl 50% TALONR beads slurry (Clontech), the cell lysate was incubated at 10min at 4°C on a rolling shaker ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentrations were determined via the Bradford method using the TaKaRa Bradford Protein Assay Kit (Takara Bio, T9310A).
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bioRxiv - Plant Biology 2021Quote: Protein-protein interactions via yeast-two-hybrid (Y2H) assays were performed as described in the Matchmaker protocol (Clontech). HvRACB (amino acids 1-193 ...
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bioRxiv - Biochemistry 2023Quote: ... Uncleaved protein and cleaved His6-Ztag domains were separated from cleaved protein by incubation with TALON resin (Clontech). The supernatant was buffer exchanged to MES A buffer (20 mM MES ...
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bioRxiv - Microbiology 2020Quote: Tissue protein lysates were purchased from Takara. Details for each lysate are shown in Table S1 ...
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bioRxiv - Neuroscience 2020Quote: ... cell-free protein expression system from Takara Bio Inc ...
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bioRxiv - Biochemistry 2021Quote: ... Protein was purified using Talon resin (Clontech), eluted in 200 mM imidazole ...
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bioRxiv - Zoology 2020Quote: ... The protein concentration was measured by TaKaRa BCA Protein Assay Kit (Takara Bio ...
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bioRxiv - Molecular Biology 2019Quote: ... and protein was incubated with Talon (Clontech), 0.5 ml resin per litre cell culture for 1 h at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... the Capturem Protein A technology from Takara was employed according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... subtilis Secretory Protein Expression System (TAKARA Bio). For signal peptide library transformation into B ...
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bioRxiv - Bioengineering 2021Quote: ... VSV-G-pseudotyped lentivirus was obtained by co-transfection of Lenti-X 293T cells (Takara) with pRRL-based transfer plasmids as well as pMD2.G (Adgene # 12259 ...
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bioRxiv - Neuroscience 2022Quote: ... and VSV-G (viral envelope) (Stewart et al., 2003) into Lenti-X HEK293T cells (Clontech) at a mass ratio of 5:4:3 using polyethyleneimine (PEI) ...
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bioRxiv - Immunology 2022Quote: ... cells were transfected with pCMV-VSV-G (1.5 μg) and pRETROX TET3G (2.5 μg) (Clontech) using Lipofectamine 2000 (10 μL) ...
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bioRxiv - Microbiology 2022Quote: ... qTOWER3 G Real-Time System (Analytik Jena) Thermal Cycler Dice Real Time System III (Takara) or 7500 Real-Time PCR System (Thermo Fisher Scientific).
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bioRxiv - Immunology 2024Quote: ... and pMD2-VSV-G to HEK293T cells using the CalPhos Mammalian Transfection Kit (Clontech Laboratories). Viral supernatants were filtered through 0.45 μm low-protein-binding filters ...
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bioRxiv - Microbiology 2023Quote: ... qTOWER3 G Real-Time System (Analytik Jena) Thermal Cycler Dice Real Time System III (Takara) or 7500 Real-Time PCR System (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2023Quote: Expression plasmids for fusion proteins of GFP and tardigrade proteins were constructed by Gibson assembly into pEGFP-N1 (Clontech) of the tardigrade cDNA (obtained by gene synthesis from Integrated DNA ...
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bioRxiv - Immunology 2021Quote: ... and the supernatant was subjected to affinity chromatography using Ni-NTA beads column (Takara). Eluted protein was dialyzed against 1×PBS with four changes for two days at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant (800 µL) was incubated with 30 µL of Talon beads (Takara Bio) at 4°C for 1 hour with continuous shaking ...
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bioRxiv - Cell Biology 2019Quote: ... and both proteins were separated from each other and from a protein encoded by blasticidin resistance gene (cloned from pQCXIB #631516, Clontech) by self-cleavage peptide P2A ...