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Citations for Takara Bio :
1 - 50 of 552 citations for Protein A G Magnetic Beads for IP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: Each purified protein preparation of His-PHOT1 N2 and N4 was incubated with TALON Magnetic Beads (TaKaRa) at 4°C for 30 min and further incubated at 4°C for 30 min with in vitro transcription and translation reactant containing RPT2 N ...
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bioRxiv - Plant Biology 2020Quote: ... then incubated with TALON magnetic beads (Clontech, cat# 35636) for 20 min at 4 °C ...
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bioRxiv - Plant Biology 2020Quote: ... then incubated with TALON magnetic beads (Clontech, cat# 35636) for 20 min at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... Total protein was extracted from 2,000 midguts using the lysis buffer supplied in the Capturem IP & Co-IP kit (Takara, 635721). The extracted proteins were divided into two equal parts and used for immunoprecipitation (IP) ...
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bioRxiv - Microbiology 2023Quote: ... Amplified fragments were purified using 0.55x beads/sample ratio NucleoMag® NGS magnetic beads (Takara Bio). Amplicons were prepared for Oxford Nanopore sequencing using the ligation sequencing kit SQK-LSK110 (Oxford Nanopore Technologies ...
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bioRxiv - Genomics 2022Quote: ... followed by magnetic bead purification with NucleoMag NGS Clean-up and Size Select (Takara Bio). To prepare the CRISPEY-BAR middle insert for ligation ...
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bioRxiv - Biochemistry 2022Quote: ... pro-meprin α was activated by trypsin cleavage applying immobilized trypsin on magnetic beads (PT3957-1, Takara, buffer 30 mM Tris-HCl pH 7.4 ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amounts of protein were incubated with TALON beads (Clontech) pre-equilibrated with purification buffer at RT for 1.5 h with overhead rotation ...
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bioRxiv - Genomics 2019Quote: ... and proteins extracted with affinity purification using Cobalt-Talon beads (Clontech). Site-directed mutagenesis for Exd and Hth was performed via amplification of the original plasmid with primers harboring single amino acid replacements (arginine to alanine ...
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bioRxiv - Biochemistry 2023Quote: ... MA) and the protein was purified using Talon beads (Takara Bio Inc., Japan) following published protocols 37,38 ...
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bioRxiv - Cell Biology 2023Quote: ... for GST-tagged protein or on TALON-Cobalt beads column (635507, Takara Bio) for 6XHIS-tagged protein as previously described [19,26] ...
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bioRxiv - Bioengineering 2023Quote: ... The process was performed on a 200 μL scale using 160 μL of His60 Ni-magnetic beads suspension (Takara Bio, Shiga, Japan). The beads were washed twice with 500 μL of sterilized water and 500 μL of Equilibration buffer (20 mM Tris-HCl pH 7.4 ...
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Pyruvate kinase M2 regulates Japanese encephalitis virus replication by interacting with NS1 proteinbioRxiv - Microbiology 2024Quote: Capturem Co-IP kit was procured from TaKaRa (Japan). The Neuro-2a cells were transfected with pEGFP-PKM2 and cells were infected with JEV 24 hours post-transfection ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant proteins of interest were bound to TALON metal affinity beads (Clontech, Mountain View, CA) and eluted with imidazole (gradient 10–200 mM ...
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Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: ... His6-tagged protein in the supernatant was captured on Talon Sepharose beads (#635502, Clontech/Takara) and pre-equilibrated against Buffer1 (300 mM NaCl ...
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Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: ... His6-tagged protein in the supernatant was captured on Talon Sepharose beads (#635502, Clontech/Takara) and pre-equilibrated against Buffer1 (300 mM NaCl ...
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bioRxiv - Developmental Biology 2019Quote: ... for RNA-sequencing and IP or using RNAiso Plus (Takara Bio) for analysis by qRT-PCR as per the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... with iPS cell Generation Episomal vector Mix (Takara Bio, Shiga, Japan) and Amaxa Human CD34+ Cell Nucleofector kit (Lonza ...
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bioRxiv - Neuroscience 2023Quote: ... lysates were centrifuged at 31,000 x g for 45 min at 4°C and the supernatant was mixed with nickel-nitrilotriacetic acid (Ni-NTA) beads (Takara, 635653) at 4°C for 2 h ...
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bioRxiv - Microbiology 2023Quote: ... The crude lysates were centrifuged at 35’000 g for 30 min and the cleared lysates incubated with 200 μL Ni-NTA agarose beads (Takara Bio, Shiga, Japan) for 1h at 4 °C ...
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bioRxiv - Genomics 2019Quote: ... the beads were washed twice with 50 μl 80% freshly-prepared EtOH on the magnetic stand and the dried beads pellets were resuspended in 4 μL elution buffer (RNAse inhibitor 1/200 Takara, CDS primer 0.5 μL in RNAse free water) immediately followed by an incubation at 72°C for 3 min ...
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bioRxiv - Microbiology 2023Quote: ... The protein-bead mixtures were then loaded onto a gravity column (TALON 2 mL Disposable Gravity Column, Takara #635606). Lysate was loaded into the column 5 times with gravity flow and then washed in a 1M NaCl buffer 3 times ...
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bioRxiv - Biochemistry 2024Quote: ... The other 2A proteins were separated from the lysate using immobilized metal affinity chromatography with either TALON beads (Clontech) or chelating Sepharose beads charged with NiCl2 (Cytiva) ...
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bioRxiv - Cell Biology 2020Quote: The commercially available Cellartis Human iPS Cell Line 12 (Takara Bio Inc., Kusatsu, Japan) was used as the human iPS Cell line ...
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bioRxiv - Immunology 2021Quote: ... using the 1.5mL centrifuge tube magnetic stand (Takara, #621964). The final library concentrations and qualities were determined by both SYBR PCR (Kapa Biosystems ...
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bioRxiv - Physiology 2022Quote: ... the beads were well-washed by column binding buffer 5 times and then incubated in 2× Protein SDS PAGE Loading (Takara) 100°C for 5 min to completely elute the proteins ...
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bioRxiv - Molecular Biology 2019Quote: ... The cell debris was removed upon centrifugation and the proteins were purified from the supernatant by His-tag affinity chromatography using Ni-NTA agarose beads (Clontech). The bound proteins were washed with lysis buffer containing 10 mM imidazole and then eluted with lysis buffer containing 250 mM imidazole ...
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bioRxiv - Biochemistry 2019Quote: ... The cell debris was removed upon centrifugation in a SS34 rotor at 4 °C (27,000 × g for 15 min) and the proteins were purified from the supernatant by His-tag affinity chromatography using Ni-NTA agarose beads (Clontech). The bound proteins were washed with lysis buffer containing 25 mM imidazole and then eluted with lysis buffer containing 500 mM imidazole ...
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bioRxiv - Developmental Biology 2022Quote: ... Co-IPs were carried out by incubating the samples with 30 μL of protein A agarose bead slurry for 4h at 4°C in a rotating wheel and with anti-mCherry (Takara 632496) of 1:1000 dilution ...
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bioRxiv - Genetics 2019Quote: ... input and IP library were constructed using SMARTer® Stranded Total RNA-seq Kit (Takara, 635007), respectively ...
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bioRxiv - Cell Biology 2020Quote: ... and pVSV-G (Takara Bio), followed by viral amplification in cells for 72 hours and addition of viral particles to HeLa cells ...
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bioRxiv - Cell Biology 2022Quote: ... pVSV-G (PT3343-5, Clontech) and psPAX2 (Addgene plasmid #12260 ...
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bioRxiv - Cell Biology 2024Quote: ... pVSV-G (PT3343-5, Clontech) and psPAX2 (Addgene plasmid #12260 ...
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bioRxiv - Cell Biology 2023Quote: ... applying their current hepatocyte differentiation protocol (Cellartis iPS Cell to Hepatocyte Differentiation System, Cat. No. Y30055, Takara Bio Europe AB ...
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bioRxiv - Cancer Biology 2020Quote: ... were transfected with pVSV-G (Clontech) and a pBABE vector containing both a gene for YFP-tagged histone H2B protein and for a gene encoding G418 resistance ...
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bioRxiv - Cancer Biology 2020Quote: ... 2.2 μg pCMV5-VSV-G (Clontech) were diluted in 600 μl OptiMem (Gibco ...
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bioRxiv - Immunology 2020Quote: ... and 160 ng pDM2.G (Clontech), 1.3 μg CMV 8.91 (Clontech) ...
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bioRxiv - Bioengineering 2023Quote: ... 2 µg pantropic pVSV-G (Clontech), 3 µg pCL- (Imgenex) ...
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bioRxiv - Cell Biology 2023Quote: ... and pVSV-G (#PT3343-5, Clontech) vectors were transfected using Lipofectamine 3000 ...
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bioRxiv - Bioengineering 2023Quote: ... GFP BrightComp eBeads™ Compensation Bead Kit (ThermoScientific) and mCherry Flow Cytometer Calibration Beads (Takara). Flow cytometry of stained T lymphocytes was then performed using a BD LSRFortessa Cell Analyzer (BD Biosciences ...
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bioRxiv - Neuroscience 2021Quote: ... High quality samples from matched input and IP fractions were prepared using the (Low-input Library Prep kit (Clontech). Sequenced reads were mapped with STAR aligner (Dobin et al. ...
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bioRxiv - Neuroscience 2020Quote: ... The resulting input and neuronal IP samples were used for isolation of total RNA using Nucleospin RNA XS kit according to manufacturer’s protocol (Takara).
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bioRxiv - Cell Biology 2020Quote: Constructs used for HEK 293T IP studies were cloned into pcDNA3.1 vector using PCR-based In-Fusion HD cloning (Clontech). KIF5 (pBa-GFP-lnkr-mmKIF5A ...
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bioRxiv - Cell Biology 2022Quote: ... pVSV-G (631530, Clontech, Mountain View, CA) and psPAX2 ...
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bioRxiv - Biochemistry 2022Quote: Co2+-bound NTA-sepharose beads (Takara) were stripped off the metal ion with 100 mM EDTA and used as NTA beads or charged with 100 mM NiCl2 and used as NTA(Ni2+ ...
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bioRxiv - Plant Biology 2024Quote: ... A glutathione sepharose beads (635608, Takara)-based purification method was then used to purify the fusion protein ...
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bioRxiv - Cell Biology 2023Quote: ... Retroviruses with the vesicular stomatitis virus–G (VSV-G) envelope were produced by transfection of GP2-293 cells (Clontech) with the pRetroX construct and pVSV-G (Clontech ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was then mixed with 50 µL cobalt beads (TALON® Metal Affinity beads Clontech, 635502) prewashed three times in guanidium hydrochloride buffer and the samples incubated at RT for 3 h with rotation ...
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bioRxiv - Molecular Biology 2021Quote: ... both input and IP samples were used for library construction with the SMARTer Stranded Total RNA-seq Kit v2 (634413, Takara), and sequenced by Illumina HiSeq X Ten to produce 150 bp paired-end reads.
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bioRxiv - Molecular Biology 2019Quote: ... RNA isolation was performed using Trizol and 50 ng of input or IP RNA were used for rRNA removal using RiboGone - Mammalian (Clontech) and library preparation using the SMARTer stranded RNA-seq Kit (Clontech) ...