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Citations for Takara Bio :
1251 - 1300 of 3888 citations for Recombinant Mouse Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2022Quote: ... The blocked membrane was incubated overnight at 4 ℃ in primary antibodies: mouse polyclonal anti-Cas9 (Takara Bio, cat. no. 632607), mouse monoclonal anti-β-actin (8H10D10 ...
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bioRxiv - Genetics 2019Quote: ... liver and kidney), mouse tissues (brain, liver, heart, testis and kidney) and rat tissues (brain, liver and kidney) were purchased from Takara Bio Inc.
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bioRxiv - Neuroscience 2021Quote: ... The cDNAs encoding full-length human HCN1 channel and mouse TRIP8b (splicing variant 1a-4) were cloned into the pcDNA 3.1 (Clontech Laboratories) mammalian expression vector ...
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bioRxiv - Neuroscience 2022Quote: Total RNA from mouse cochleae was extracted using an RNA Extraction Kit according to the manufacturer’s instructions (#9767, TaKaRa, Japan). The extracted RNA concentration was measured using a Nanodrop 2000 spectrophotometer (#ND-LITE ...
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bioRxiv - Cell Biology 2019Quote: ... of mouse E2F1 gene and the resultant fragment was ligated between the BamH1 and Xba1 sites of the pQXCIP vector (Clontech). pQCXIP/N-m (or h)E2F1p::pE2VF1-3’UTR ...
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bioRxiv - Neuroscience 2021Quote: Total RNAs from the mouse forebrain were reverse transcribed using the PrimeScript II first strand cDNA Synthesis Kit (Takara Bio) with the random hexamer primer ...
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bioRxiv - Immunology 2020Quote: ... 10ng of RNA was used to prepare bulk TCR-seq libraries using the SMARTer Mouse TCR a/b Profiling Kit (Takara) according to instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The samples were subjected to 4-20% SDS-PAGE and analyzed by immunoblotting with the following primary antibodies: anti-GFP mouse monoclonal antibody (JL-8, Takara), GFP rabbit polyclonal antibody (PABG1 ...
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bioRxiv - Neuroscience 2022Quote: ... These plasmids were obtained by cloning cDNA extracted from mouse cerebellum in pAAV-MCS Expression Vector with In-Fusion Cloning system (Takara). 96h after transfection ...
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bioRxiv - Cell Biology 2024Quote: We cloned a 1.6 kb genomic region upstream from start codon of mouse Lmna as the endogenous promoter (-1407 to +249 bp from transcription start site) using PrimeSTAR HS DNA Polymerase (Takara). Primers used in this study are listed in Table S2 ...
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bioRxiv - Immunology 2024Quote: The full-length cDNA of mouse CCL2 (NM_011333.3) was amplified by PCR with PrimeSTAR Max DNA Polymerase (Takara Bio, #R045A) from mouse MCP-1/CCL2 cDNA ORF Clone (Sino Biological ...
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bioRxiv - Biophysics 2023Quote: ... A plasmid expressing mouse GR tagged in the N-terminus with mCherry was developed by amplifying mouse GR coding sequence and in-frame cloning in pmCherry-C3 (Clontech). Point mutations and deletions were introduced using the Quickchange XL mutagenesis kit (Agilent ...
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bioRxiv - Biochemistry 2023Quote: ... was generated by amplifying full-length mouse ANGPTL4 cDNA (OpenBiosystems) and inserting it into a pCDNA6 vector using In-Fusion cloning (Clontech). A V5 tag was then appended to the C terminus of the open reading frame using Phusion site-directed mutagenesis (New England Biolabs).
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bioRxiv - Developmental Biology 2024Quote: ... cDNA encoding ZP3 was restored from the mouse ovarian tissue by RT-PCR using PrimeScript RT Reagent Kit (Takara, RR037). Two PCR amplicons including the 5′ region of the TECTA-ZP and the transmembrane domain (TMD ...
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bioRxiv - Genetics 2023Quote: Full-length mouse Rnf212b and Rnf212 cDNAs were amplified by PCR from mouse testis cDNA prepared as above and cloned into pGADT7 and pGBKT7 vectors (Clontech) using the Gibson Assembly Cloning Kit (NEB ...
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bioRxiv - Immunology 2023Quote: ... A cDNA library for TCR was prepared from RNA using a SMARTer Mouse TCR a/b Profiling Kit (Takara Bio) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Blots were probed for 1h at room temperature (RT) with a mouse anti-GFP antibody (Living Colors -JL-8, BD Biosciences Clontech) in TBS-T buffer (20 mM Tris ...
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bioRxiv - Microbiology 2023Quote: Sequence of the mouse variable heavy and kappa chains were obtained by using SMARTer 5’ RACE technology (Takara Bio, USA) adapted for antibodies to amplify the variable genes from heavy and kappa chains for each hybridoma based on isotype ...
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bioRxiv - Cell Biology 2024Quote: ... and the presence of ΔTM-VezA-GFP was confirmed by western blotting analysis with a mouse monoclonal anti-GFP antibody from Clontech. In addition ...
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bioRxiv - Cell Biology 2024Quote: For the phogrin-Dronpa-Green1 and phogrin-EGFP expression vectors, mouse phogrin cDNA was ligated with pDronpa-Green1 (MBL, Japan) or pEGFP-N1 (Clontech), and transferred to the ViraPower Adenoviral Expression System (Thermo Fisher).
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bioRxiv - Cell Biology 2020Quote: ... 7.5 × 107 Hek293-lentiX cells (Clontech) were seeded on 15-cm tissue culture plates ...
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bioRxiv - Genetics 2021Quote: ... Lenti-X™ 293T Cells (Takara) were seeded on 10 cm dishes (6 × 106 cells per dish) ...
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bioRxiv - Developmental Biology 2021Quote: ... coli Stellar chemically competent cells (TaKaRa) were sequenced prior to transformation of the BL21(DE3 ...
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bioRxiv - Microbiology 2022Quote: ... Addgene_12259) into 293XT cells (Takara Holdings). Lentivirus containing supernatant was harvested at 48 ...
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bioRxiv - Molecular Biology 2020Quote: Lenti-X 293T cells (Clontech, 632180) were cultured for at least 24 hours in DMEM supplemented with 10% FBS and lacking antibiotics ...
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bioRxiv - Immunology 2021Quote: ... Lenti-X 293T cells from Takara Bio (632180 ...
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bioRxiv - Synthetic Biology 2019Quote: Supercompetent Escherichia coli cells (Stellar, TaKaRa) were used for construction of all relevant plasmids and were cultured in LB medium ...
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bioRxiv - Developmental Biology 2020Quote: ... Lenti-X 293T cells (Clontech, 632180) were transfected with appropriate amounts of lenti-viral constructs ...
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bioRxiv - Cell Biology 2019Quote: Tet-on HepG2 cells (Clontech-Takara) were seeded on poly-lysine coated glass cover slips in 12-well plates at 0.7×105 cells/well and grown in MEM/EBSS with 10% FBS and penicillin/streptomycin at 37°C with 5% CO2 ...
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bioRxiv - Cell Biology 2019Quote: Tet-on HepG2 cells (Clontech-Takara) were seeded on poly-lysine coated glass cover slips in 12-well plates at 0.7×105 cells/well and grown in MEM/EBSS with 10% FBS and penicillin/streptomycin at 37°C with 5% CO2 ...
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bioRxiv - Cancer Biology 2020Quote: ... retroviral Packaging Cells (GP2-293; Clontech) were transfected with pVSV-G (Clontech ...
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bioRxiv - Biochemistry 2021Quote: Lenti-X 293T cells (Takara bio) grown in a 100 mm plate format were co-transfected with 4.5 μg psPAX2 (Didier Trono lab ...
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bioRxiv - Neuroscience 2021Quote: ... in Lenti-X 293T cells (Takara). Viral supernatant was collected at 48 and 72 hours post transfection and concentrated using Lenti-X Concentrator (Takara) ...
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bioRxiv - Microbiology 2021Quote: ... Competent AH109 yeast cells (Clontech, USA) were prepared and transformed with the Y2H plasmid constructs by using the Frozen-EZ Yeast Transformation II kit (Zymo Research ...
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bioRxiv - Immunology 2021Quote: ... coli STELLAR chemically competent cells (TaKaRa) and E ...
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bioRxiv - Microbiology 2021Quote: ... Lenti-X 293T cells (Takara #632180) were used for lentivirus packaging ...
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bioRxiv - Biochemistry 2020Quote: ... Stellar Competent Cells were from Takara and One Shot® BL21 (DE3 ...
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bioRxiv - Immunology 2022Quote: Lenti-X 293T cells (Takara Bio) were transfected with pHR SIN including cloned transgene and packaging vectors pMD2.G and pCMVdR8.91 using TransIT-Lenti Transfection Reagent (Mirus #MIR6603) ...
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bioRxiv - Genomics 2019Quote: Lenti-X 293T cells (Takara Biosciences) were used for lentivirus production ...
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bioRxiv - Immunology 2022Quote: ... Lenti-XTM 293T cells (Takara Bio) in DMEM media containing 10% FBS and 100 U/ml Pen/Strep were transfected using the standard calcium phosphate method with 15 μg of the CER transfer vector ...
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bioRxiv - Cancer Biology 2019Quote: 293GP cells (Clontech, Mountain View, CA) were seeded to 70% confluence in DMEM medium containing 10% fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2019Quote: ... into Lenti-X 293T cells (Clontech). Lenti-X 293T cells were seeded at 70% confluence to one 10 cm-dish 8 hours before transfection to reach about 90% confluence before transfection ...
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bioRxiv - Neuroscience 2020Quote: ... Lenti-X HEK 293T cells (ClonTech) were used for producing lentiviral particles ...
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bioRxiv - Cell Biology 2019Quote: ... retinal-pigmented epithelial cells (RPE1; Clontech) and RPE1 expressing LifeAct-GFP (Vignaud et al. ...
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Increased ACTL6A Occupancy Within mSWI/SNF Chromatin Remodelers Drives Human Squamous Cell CarcinomabioRxiv - Molecular Biology 2021Quote: ... HEK293T cells were purchased from Takara Bio USA (632180 ...
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bioRxiv - Immunology 2021Quote: ... Lenti-X 293T cells (Takara, 632180) were seeded in 10-cm2 dishes at a density of 5e6 cells per dish c and the following day transfected with 10 μg of spike expression plasmid with TransIT-Lenti (Mirus ...
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bioRxiv - Developmental Biology 2021Quote: ... Lenti-X 293T cells (Clontech, 632180) were cultured in DMEM (containing 1% penicillin/streptomycin ...
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bioRxiv - Microbiology 2021Quote: ... Lenti-X 293T cells (Takara, 632180) were seeded in 10-cm dishes at a density of 1e5 cells/cm2 and the following day transfected with 5 μg of spike expression plasmid with TransIT-Lenti (Mirus ...
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bioRxiv - Microbiology 2021Quote: ... Lenti-X 293T cells (Takara, 632180) were seeded in 10-cm2 dishes at a density of 5e6 cells per dish and the following day transfected with 10 μg of WT or B.1.617.2 spike expression plasmid with TransIT-Lenti (Mirus ...
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bioRxiv - Cancer Biology 2021Quote: ... HEK293T cells and LentiX Concentrator (Clontech) were used to generate and concentrate lentiviral particles ...