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Citations for Takara Bio :
1151 - 1200 of 3662 citations for Recombinant Mouse Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... 2 µg of total RNA was subsequently reverse transcribed to cDNA using PrimeScript RT Master Mix (Takara Bio) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2022Quote: ... cDNA was synthesized using 2 μg RNA using the PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa). Gene specific primers for quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Plant Biology 2021Quote: ... 2 μg of RNA was reverse transcribed in a 20 μL volume with RT PCR master mix (TaKaRa) as per the manual instruction ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR was then performed using gene-specific primers (Table 2) and TB Green Premix Ex Taq (Takara). Levels of gene expression were normalized to that of 16S rRNA and expression was assessed for each biofilm and each timepoint relative to its planktonic counterpart using the 2−ΔΔCt method (43).
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bioRxiv - Evolutionary Biology 2022Quote: ... Yeast 2-hybrid library screening was conducted using the Matchmaker Gold Yeast Two-Hybrid System (Takara Bio USA) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: Purified total RNA was reverse transcribed into cDNA by PrimeScript™ 2 1st strand cDNA Synthesis Kit (TaKaRa). The target gene fragments (Brachyury ...
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bioRxiv - Systems Biology 2019Quote: ... the samples were added to 2 μL of second-strand synthesis mix (2.5× NEB buffer 2 [New England Biolabs], 0.625 mM each dNTP Mixture [TaKaRa] ...
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bioRxiv - Microbiology 2020Quote: ... The qPCR reaction system consisted of 12.5 μL of 2 × SYBR Premix Ex TaqTM II (Takara, Dalian, China), 0.5 μL of upstream and downstream primers (10 mM) ...
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bioRxiv - Plant Biology 2021Quote: ... URA3: GAL1UAS–Gal1TATA–LacZ MEL1) via the Yeastmaker™ Yeast Transformation System 2 kit (Clontech, Mountain View, USA). An agarose gel image of the cDNA library is shown in Fig ...
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bioRxiv - Genomics 2021Quote: ... Long range PCR was performed using Advantage 2 Polymerase following the manufacturer’s protocol (Clontech Laboratories, Mountain View CA).
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... The 2-μg RNA sample was reverse transcribed using the PrimeScript™ RT Master Mix (Takara, Shiga, Japan). Quantitative real-time PCR (qPCR ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was amplified by PCR in separate 25 μL reactions each containing 2 μL of cDNA for typically 7-9 cycles using 0.5 μL (2.5 u) LA Taq (Takara) with P5-3’ and miRCat-PCR-2 oligos (IDT ...
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bioRxiv - Neuroscience 2023Quote: ... the cell body was immediately sucked into the glass electrode with negative pressure and expelled onto a 1.1 µl drop of the ice-cold lysis buffer (0.1% Triton X-100, 2 U/µl TaKaRa RNAse inhibitor ...
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bioRxiv - Synthetic Biology 2023Quote: ... Specific primers were designed for this purpose and PCR was done using the Advantage 2 Polymerase kit (Clontech). Complementary 60 bp-ends to each target sequence on all Mycoplasmas species genome used in this study ...
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bioRxiv - Cell Biology 2023Quote: ... cultures were split into two 2-ml cultures of which one was supplemented with 250 nM rapalog (Clontech). Mislocalisation of the target protein was verified by live-cell microscopy.
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bioRxiv - Cell Biology 2023Quote: ... and N-SREBP2 (1440 bps) were amplified using high-fidelity PCR (Advantage-HF 2 PCR kit, #639123; Clontech) from human pcDNA3.1-2xFLAG-SREBP1a (#26801 ...
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bioRxiv - Genetics 2023Quote: ... electrophoresis was performed using 9 μL of the PCR products on a 2% agarose gel (Takara Bio, Japan) at 100 V ...
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bioRxiv - Cell Biology 2023Quote: ... The virus titer was quantified using the AAVpro Titration Kit (for Real Time PCR) Ver.2 (Takara Bio) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... 1 × GC buffer II and 1 U of LA Taq DNA polymerase (TaKaRa). The PCR cycles consisted of 95 °C for 5 min ...
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bioRxiv - Biochemistry 2022Quote: ... TEX12 (1-123) and SIX6OS1 (1-587) were cloned into pGADT7 vectors (Clontech). The Matchmaker™ Gold system (Clontech ...
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bioRxiv - Molecular Biology 2020Quote: ... The plug was equilibrated twice in 1 ml of 1× M buffer (TaKaRa) by rotating the tube for 30 min at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... then in 1:25,000 or 1:100,000 rabbit anti DSRed (Takara Bio USA) in 10% NHS-TPBS ...
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bioRxiv - Microbiology 2022Quote: ... and 50 ng mL−1 or 25 ng mL−1 anhydrotetracycline (aTc, Clontech) and Aeromonas sp ...
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bioRxiv - Molecular Biology 2024Quote: ... The plug was equilibrated twice in 1 mL of 1× M buffer (TaKaRa) by rotating the tube for 30 min at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... The plug was equilibrated twice in 1 mL of 1× M buffer (TaKaRa) by rotating the tube for 30 min at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were harvested by centrifugation and cell pellets were lysed through xTractor Lysis Buffer (Takara, Japan) supplemented with 20 mM imidazole ...
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bioRxiv - Cancer Biology 2023Quote: GFP-expressing HPAF-II cells were engineered via second-generation lentiviral transfection with LX293T cells (Takara) using pLX302-EGFP plasmid (Kevin Janes ...
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bioRxiv - Cell Biology 2020Quote: ... 7.5 × 107 Hek293-lentiX cells (Clontech) were seeded on 15-cm tissue culture plates ...
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bioRxiv - Genetics 2021Quote: ... Lenti-X™ 293T Cells (Takara) were seeded on 10 cm dishes (6 × 106 cells per dish) ...
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bioRxiv - Developmental Biology 2021Quote: ... coli Stellar chemically competent cells (TaKaRa) were sequenced prior to transformation of the BL21(DE3 ...
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bioRxiv - Microbiology 2022Quote: ... Addgene_12259) into 293XT cells (Takara Holdings). Lentivirus containing supernatant was harvested at 48 ...
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bioRxiv - Molecular Biology 2020Quote: Lenti-X 293T cells (Clontech, 632180) were cultured for at least 24 hours in DMEM supplemented with 10% FBS and lacking antibiotics ...
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bioRxiv - Immunology 2021Quote: ... Lenti-X 293T cells from Takara Bio (632180 ...
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bioRxiv - Synthetic Biology 2019Quote: Supercompetent Escherichia coli cells (Stellar, TaKaRa) were used for construction of all relevant plasmids and were cultured in LB medium ...
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bioRxiv - Developmental Biology 2020Quote: ... Lenti-X 293T cells (Clontech, 632180) were transfected with appropriate amounts of lenti-viral constructs ...
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bioRxiv - Cell Biology 2019Quote: Tet-on HepG2 cells (Clontech-Takara) were seeded on poly-lysine coated glass cover slips in 12-well plates at 0.7×105 cells/well and grown in MEM/EBSS with 10% FBS and penicillin/streptomycin at 37°C with 5% CO2 ...
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bioRxiv - Cell Biology 2019Quote: Tet-on HepG2 cells (Clontech-Takara) were seeded on poly-lysine coated glass cover slips in 12-well plates at 0.7×105 cells/well and grown in MEM/EBSS with 10% FBS and penicillin/streptomycin at 37°C with 5% CO2 ...
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bioRxiv - Cancer Biology 2020Quote: ... retroviral Packaging Cells (GP2-293; Clontech) were transfected with pVSV-G (Clontech ...
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bioRxiv - Biochemistry 2021Quote: Lenti-X 293T cells (Takara bio) grown in a 100 mm plate format were co-transfected with 4.5 μg psPAX2 (Didier Trono lab ...
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bioRxiv - Neuroscience 2021Quote: ... in Lenti-X 293T cells (Takara). Viral supernatant was collected at 48 and 72 hours post transfection and concentrated using Lenti-X Concentrator (Takara) ...
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bioRxiv - Microbiology 2021Quote: ... Competent AH109 yeast cells (Clontech, USA) were prepared and transformed with the Y2H plasmid constructs by using the Frozen-EZ Yeast Transformation II kit (Zymo Research ...
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bioRxiv - Immunology 2021Quote: ... coli STELLAR chemically competent cells (TaKaRa) and E ...
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bioRxiv - Microbiology 2021Quote: ... Lenti-X 293T cells (Takara #632180) were used for lentivirus packaging ...
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bioRxiv - Biochemistry 2020Quote: ... Stellar Competent Cells were from Takara and One Shot® BL21 (DE3 ...
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bioRxiv - Immunology 2022Quote: Lenti-X 293T cells (Takara Bio) were transfected with pHR SIN including cloned transgene and packaging vectors pMD2.G and pCMVdR8.91 using TransIT-Lenti Transfection Reagent (Mirus #MIR6603) ...
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bioRxiv - Genomics 2019Quote: Lenti-X 293T cells (Takara Biosciences) were used for lentivirus production ...
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bioRxiv - Immunology 2022Quote: ... Lenti-XTM 293T cells (Takara Bio) in DMEM media containing 10% FBS and 100 U/ml Pen/Strep were transfected using the standard calcium phosphate method with 15 μg of the CER transfer vector ...
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bioRxiv - Cancer Biology 2019Quote: 293GP cells (Clontech, Mountain View, CA) were seeded to 70% confluence in DMEM medium containing 10% fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2019Quote: ... into Lenti-X 293T cells (Clontech). Lenti-X 293T cells were seeded at 70% confluence to one 10 cm-dish 8 hours before transfection to reach about 90% confluence before transfection ...
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bioRxiv - Neuroscience 2020Quote: ... Lenti-X HEK 293T cells (ClonTech) were used for producing lentiviral particles ...