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Citations for Takara Bio :
1201 - 1250 of 1581 citations for Phospho AMPK alpha 1 S496 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... KhES-1 was cultured at feeder-free conditions using StemFit® AK02N (Catalog #RCAK02N, TAKARA BIO, Kusatsu, Japan) with daily medium change ...
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bioRxiv - Genetics 2024Quote: ... 1 μg of total RNA was reversely transcribed into cDNA using the Prime Script RT kit (Takara, RR047A). The SYBR Green qPCR master mix (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Programed cell death was induced by incubating cells in complete DMEM with 1 mM B/B homodimerizer (Clontech) for 15 min at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... the 99-1820 bp fragment of the Rat Glt-1 plasmid (GenBankTM accession number X67857.1) was subcloned between the EcoRI and Xba restriction sites of pEGFP vector (Clontech). GFP-Rab4 ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 g RNA was used to synthesize the cDNA using reverse transcription M-MLV (RNase-free) kits (TaKaRa, Japan). qRT-PCR was performed with SYBR green PCR master mix (ToYoBo ...
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bioRxiv - Plant Biology 2019Quote: ... proteins were transferred to PVDF membrane and blotted proteins were incubated in a 1:10,000 dilution of mouse monoclonal anti-GFP antibody (Living Colors JL-8, Clontech) followed by 1:5000 horseradish peroxidase conjugated sheep anti-mouse IgG antibody (Amersham ...
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bioRxiv - Cancer Biology 2019Quote: ... The RNA (1 μg) was applied to generate cDNA by means of PrimeScript™RT Master Mix (Takara, RR036A). The real-time quantitative PCR was performed by using SYBR Green Master Mix (Takara ...
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bioRxiv - Cell Biology 2019Quote: ... For lentiviral transduction WT and Quad-K Jam-C-GFPout were amplified using appropriate primers (Supp. Table 1) and subcloned into pSFFV-WPRE using an infusion reaction according to the manufacturer’s instructions (Clontech).
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bioRxiv - Genomics 2019Quote: ... We spun down the aliquots and re-suspended the pellets in 1 ml DroNc-seq resuspension buffer (1X PBS, 0.01% BSA, 0.16 U/μl RNase Inhibitor (Clontech/TaKaRa, #2313A)) for Tube A and 1 ml 10X Nuclei resuspension buffer (1X PBS ...
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bioRxiv - Microbiology 2021Quote: ... Reverse transcription was carried out with 1 μg RNA using TB Green Premix Ex Taq™ II (Takara, Japan). Targets of around 110 bp were amplified with primers qtaxB-F/R ...
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bioRxiv - Cell Biology 2021Quote: ... PCR was performed using 1–2 μL of the genomic DNA solution and Tks Gflex DNA polymerase (Takara Bio). The primers are listed in Table S3 ...
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Panacea: a hyperpromiscuous antitoxin protein domain for the neutralisation of diverse toxin domainsbioRxiv - Microbiology 2021Quote: ... Filtered lysate was incubated with 1 mL of previously buffer equilibrated Ni-beads (His60 Ni Superflow Resin, TaKaRa, Japan) for 30 minutes ...
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bioRxiv - Microbiology 2021Quote: The LTR promoter of the HIV-1 laboratory strain pNL4-3 was cloned into the pTA-Luc backbone (Clontech) and is henceforth referred to as pTA-Luc-NL4-3 ...
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bioRxiv - Cell Biology 2021Quote: ... Total RNA (1 μg) was converted to cDNA using the PrimeScript™ RT reagent Kit with gDNA Eraser (TAKARA). Quantitative PCR (qPCR ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were washed twice with 20 mL of D-PBS (Nacalai Tesque) and resuspended in CELLBANKER 1 (Takara Bio) at 1 × 107 cells/mL ...
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bioRxiv - Microbiology 2022Quote: ... The cDNA was prepared from 1 μg of total RNA using PrimeScript 1st strand cDNA Synthesis Kit (TaKaRa, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... The cDNA was amplified with gene-specific primers (Supplemental Table 1) and SYBR Premix Ex Taq II kit (TaKaRa). Data were analyzed using a 2−ΔΔCt method.
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bioRxiv - Genetics 2021Quote: ... cDNA synthesis was done using 0.1-1 μg approximately of RNA following the manufacturer protocol (Takara Bio, Kusatsu, Japan). We used the 2xPrimeTime® Gene Expression Master Mix (Takara Bio) ...
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bioRxiv - Cancer Biology 2021Quote: ... with PCR using the primers listed in Table 1 and cloned into pmCherry-C1 (CMV promoter; Takara Bio Inc.). pEGFP-C3 K19 WT ...
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bioRxiv - Biophysics 2021Quote: ... The resulting supernatant was collected by ultracentrifugation (150,000 × g, 1 hour, 4 °C) and applied onto an immobilized metal-ion affinity chromatography column (Talon, Clontech) equilibrated with 40 mM Tris ...
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bioRxiv - Genetics 2022Quote: ... 1 µg of total RNA was used for reverse transcription using PrimeScript RT Reagent Kit (Cat#RR037A, TaKaRa, Japan). Quantitative polymerase chain reaction (qPCR ...
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bioRxiv - Cell Biology 2019Quote: ... Infected cells were cultured on Geltrex in NPC medium with 1 × RevitaCell supplement and 2 ug/ml Doxycycline (Clontech) to induce NGN2 gene expression ...
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bioRxiv - Cell Biology 2019Quote: ... 10 µg of the lysates were separated by SDS-PAGE and blots were probed with a 1:2,000 dilution of mouse anti-GFP antibody (Clontech), followed by 1:5000 anti-mouse Starbright Blue 700 (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were analyzed on bleach agarose gels (0.06% bleach, 1% (w/v) agarose) for visualization of the RNA and on WB (anti-His antibody, Clontech) for visualization of His6-tagged Nsp1.
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bioRxiv - Pathology 2020Quote: ... A total of 1 × 106 cells were used for total RNA extraction using RNAiso Plus reagent (Cat.#9109, TaKaRa), followed by treatment with 10 U of recombinant DNase I (Cat.#2270A ...
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bioRxiv - Molecular Biology 2021Quote: ... The insoluble material was removed by centrifugation at 150,000 ×g and the supernatant was added to 1 ml pure TALON resin (Clontech) and 20 mM imidazole and rock slowly overnight at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... 35 mm wells of MIN6 cells were co-transfected with 2.5 µg of pX330 containing gRNAs and 1 µg of pEGFP-C2 (Clontech). After 48 h ...
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bioRxiv - Microbiology 2020Quote: ... primary antibody was prepared in PBS containing 1% non-fat milk using anti-hexahistidine antibody (Takara Bio, catalog #631212) at a dilution of 1:3000 ...
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bioRxiv - Microbiology 2020Quote: ... 1) were annealed to a complementary 20-nt primer containing a 5’-fluorescein label (5’FAM-GUCAUUCUCCUAAGAAGCUA-3’, Takara). To perform the primer extension assay ...
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bioRxiv - Genomics 2021Quote: ... or SL medium (for E14-STNΔTsixP) and transduced the next day with 1ml of 5:1 concentrated (lenti-X, Clontech) and filtered viral supernatant with 8 ng/µl polybrene (Sigma Aldrich) ...
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Mck1 defines a key S-phase checkpoint effector in response to various degrees of replication threatsbioRxiv - Molecular Biology 2019Quote: ... DNA was transferred to HyBond N+ in transfer buffer (0.4 M NaOH, 1 M NaCl) and UV cross-linked before hybridization with a random primed probe (Takara) overnight at 42°C and washed twice for 20 min with 0.5× SSC 0.1% SDS at 65°C.
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bioRxiv - Cell Biology 2021Quote: ... 1-2ug of RNA was used to synthesise cDNA using Superscript III or PrimeScript kit (Thermo Fisher Scientific, Takara). Quantitative PCR (qPCR ...
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bioRxiv - Genomics 2021Quote: ... Non-tissue culture treated 12-well plates were prepared by incubating 1 mL PBS + 25 μg/mL Retronectin (Takara) overnight at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... The cDNA was synthesized with 1 ug total RNA using the PrimeScriptTM RT Reagent Kit with gDNA Eraser (TaKaRa). qRT-PCR was performed using a qTOWER3G system (analytikjena ...
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bioRxiv - Immunology 2020Quote: ... non-tissue culture treated 96-well plates were coated overnight at 4 °C with 1 ml of retronectin (Takara) at 25μg/ml in PBS ...
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bioRxiv - Plant Biology 2021Quote: ... The samples were immobilized by molten 1.5% low-melting point agarose (Agarose LMT 1-20K, PrimeGel, Takara Bio Inc.) spread thinly on a glass-bottom dish and immediately covered in water ...
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bioRxiv - Immunology 2020Quote: ... 1 μg of RNA per sample was used for first-strand synthesis by SMARTer PCR cDNA Synthesis Kit (Clontech). For quantitative RT-PCR (qPCR) ...
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bioRxiv - Immunology 2021Quote: ... 100,000 cells were sorted into 200 uL PBS with 1 uM DTT and 5 uL RNase Inhibitor Cocktail (Takara); for ex vivo culture experiments ...
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bioRxiv - Evolutionary Biology 2020Quote: The water-in-oil droplets after the incubation step were diluted 10000-fold with 1 mM EDTA (pH 8.0) and subjected to RT-qPCR (PrimeScript One Step RT-PCR Kit (TaKaRa)) with primer 1 and 2 after heating at 95 °C for 5 min ...
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bioRxiv - Genetics 2020Quote: ... Total RNA (1 mg) was reverse transcribed using the PrimeScript RT Reagent Kit with the gDNA Eraser (TAKARA, Japan). Quantitative PCR reactions were performed using the gene-specific primers of FLC and FT (Table S1 ...
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bioRxiv - Microbiology 2022Quote: ... A 5 µl aliquot was removed from each sample for immunoblots using mouse anti-GFP (1:5,000, Clontech #632381) to detect A3-EGFP ...
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bioRxiv - Biochemistry 2022Quote: ... MBL) and HRP-labeled IgG detector (1/5,000 dilution from the product, Western BLoT Rapid Detect v2.0, Takara Bio) were used as primary and secondary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... Complementary DNA (cDNA) was prepared from 1 μg of RNA using PrimeScriptTM RT Master Mix (catalog no. RR036A, Takara). qPCR was performed using TB Green Premix Ex TaqTM (catalog no ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The measurement was performed after diluting the droplets 100-fold with 1 mM EDTA (pH 8.0) and using One Step TB Green PrimeScript PLUS RT-PCR Kit (Takara).
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bioRxiv - Genomics 2022Quote: Sequencing libraries were prepared using 1-10 ng of cfDNA and the ThruPLEX® Plasma-seq Kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... 10 µl of RNA extraction mixture was then added to 1 µl of 10 mM dNTPs (Takara Catalog # 4030) and 100 mM of oligo-dT(18 ...
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bioRxiv - Neuroscience 2024Quote: ... HEK293 cells were first incubated with ARIAD ligand at a concentration of 1 µM (AL; D/D Solubilizer; Takara) for the indicated times ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized from 1 µg of each RNA sample using PrimeScript RT Reagent Kit with gDNA Eraser (Takara), and the primers used for qRT-PCR were designed using Primer3web ...
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bioRxiv - Microbiology 2024Quote: ... the dish was split into two 1-ml Petri dishes and one was treated with rapalog (250 nM, Clontech) for 4 hours to induce the knock-sideway while the other served as control.
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bioRxiv - Cell Biology 2023Quote: ... 2xmCh-KIF5C in pcDNA3.1 was cloned by inserting in-frame KIF5C full length from EGFP-KIF5C (a gift from Anthony Brown) 2xmCh sequence from KIF5C(1-560)-2xmCh-EF(C) into pcDNA3.1 using InFusion Cloning kit (Takara). EGFP-Rab7A (Addgene #28047 ...