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Citations for Takara Bio :
1101 - 1150 of 5257 citations for Human E3 Ubiquitin Protein Ligase SMURF2 SMURF2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... Yeast transformation as well as yeast protein extraction were performed following the recommendations presented in the Yeast Protocol Handbook PT3024-1 (Clontech).
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bioRxiv - Biophysics 2020Quote: ... 6xHis-tagged protein fraction was incubated for thirty minutes at 4°C with Talon resin (Clontech Laboratories/Takara Bio USA) that had been previously equilibrated in lysis/extraction buffer ...
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bioRxiv - Biophysics 2020Quote: ... 6xHis-tagged protein fraction was incubated for thirty minutes at 4°C with Talon resin (Clontech Laboratories/Takara Bio USA) that had been previously equilibrated in lysis/extraction buffer ...
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bioRxiv - Pathology 2021Quote: ... Expression of the GST-N protein of SARS-CoV-2 was induced by isopropyl-D-1-thiogalactopyranoside (0.3 mM IPTG, Takara Bio). The cell pellets were sonicated ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant protein were purified to >90% electrophoretic purity by immobilized metal ion affinity chromatography using a cobalt-based matrix (Talon, Clontech) and eluted with imidazole as described previously24 ...
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bioRxiv - Molecular Biology 2020Quote: PJ69-4a strain of Saccharomyces cerevisiae was used for the expression of proteins of interest using the Matchmaker Gal4-based yeast two hybrid system (Clontech). Tested bait/prey combinations were co-transformed into PJ69-4a and cultivated at 28°C ...
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bioRxiv - Biochemistry 2019Quote: Hexa-histidine-tagged scaffolding protein (6 mg) was loaded on a 1 ml immobilized metal affinity chromatography column charged with cobalt (Clontech). Coat protein monomers (0.2 mg/ml ...
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bioRxiv - Microbiology 2021Quote: ... The pcDNA3.4 expression vector containing the sequence that encodes the His-tagged extracellular domain of the spike protein was transfected into Expi293 cells and the His-tagged spike protein produced in the culture supernatants was then purified with a Talon resin (Clontech).
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bioRxiv - Cell Biology 2020Quote: ... generates a protein that undergoes spontaneous aggregation that reverses upon the addition of D/D solubilizer (catalogue# 635054; Takara Bio), a cell-permeant rapamycin analog that binds to the FM4 domains (see Fig 3e).
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bioRxiv - Immunology 2020Quote: ... For binding studies the 6xHis tag were removed from some Fabs by treatment with PreScission protease (MolBioTech; ThermoScientific) and the protein repurified on cobalt-nitrilotriacetic acid (Co-NTA) agarose (Clontech) followed by gel filtration chromatography on Superdex 200 (GE Healthcare ...
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bioRxiv - Molecular Biology 2019Quote: A DNA fragment containing CDS of each Hero protein and C-terminal FLAG and His tags was inserted into pCold I (Takara) by NEBuilder HiFi DNA Assembly Master Mix (NEB) ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmids used for expression of fluorescent proteins in hTert-RPE1 cells are derivatives of the plasmid pEGFP-C1 and pmCherry-C1 (Clontech). Plasmids were analyzed by sequencing ...
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bioRxiv - Cancer Biology 2022Quote: ... was modified to replace the puromycin-resistance gene with the enhanced green fluorescent protein (EGFP) gene using the In-Fusion Snap Assembly Master Mix (TaKaRa). Forty-eight hours after transfection ...
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bioRxiv - Microbiology 2022Quote: ... His-tagged AmiC was isolated from the protein sample using a TALON® metal affinity resin (Takara Bio USA, Inc) as previously described (51 ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 × 106 HEK293T cells were transfected with 7 μg of Env expressor and 1 μg of a green fluorescent protein (GFP) expressor (pIRES2-EGFP; Clontech) with the calcium phosphate method ...
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bioRxiv - Biophysics 2022Quote: ... These plasmids were designed by fusing the coding sequences of the respective proteins C-terminal (intracellularly) via a linker to mEGFP (called D0 / Donor only) or mCherry in the pIRESpuro2 vector (Clontech)32 (for more linker details see Supplementary Table 5) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The recombinant proteins were purified using a TALON Metal (Cobalt) Affinity Resin column (Clontech Laboratories, Inc., Palo Alto, CA, USA) and eluted with a linear gradient of imidazole (0–1,000 mM ...
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bioRxiv - Plant Biology 2023Quote: ... and the input and pull-down prey proteins were detected by immunoblot using anti-His (M201, Takara, 1:3000 dilution) and anti-GST (G018 ...
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bioRxiv - Plant Biology 2024Quote: The HIS3 and ADE2 reporter genes were employed to assess the protein interactions in the yeast strain Y2HGold (Takara Bio). Yeast transformation was carried out using the lithium acetate (LiAc)-mediated method ...
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bioRxiv - Neuroscience 2024Quote: ... HEK293 cells in 24-well plates were transfected with a total of 0.9 μg of DNA vectors carrying genes encoding GluN1 and GluN2 subunits and green fluorescent protein (GFP; pQBI 25 vector; for identification of transfected cells; Takara) in a 1:1:1 ratio using 0.9 μl of PolyMag reagent (OZBiosciences ...
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bioRxiv - Genomics 2024Quote: ... The cells were infected with prepackaged lentiviral particles (constitutive reporter vector expressing tdTomato fluorescent protein gene driven by EF1a promoter (Takara) at MOI of 20 (Stock ...
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bioRxiv - Biochemistry 2024Quote: ... Recombinant His-tagged protein was purified from cell free extracts in a single step using immobilized affinity chromatography (IMAC) with Talon resin (ClonTech) and dialyzed overnight into 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... pLysS or Rosetta pLysS cells and overexpressed proteins were purified using immobilized metal-ion affinity chromatography (IMAC) with TALON resin (Clontech) (Abad et al ...
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bioRxiv - Molecular Biology 2022Quote: cDNA was prepared from EV RNA using the Single Cell RNA kit SMART-Seq_V4 Ultra Low Input RNA Kit (634890, Takara, Mountain View, CA, USA) according to manufacturer instructions using 1ng input ...
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bioRxiv - Developmental Biology 2022Quote: ... 5’ and 3’ RACE ready cDNA was generated from a pooled female pupal RNA sample and also from a pooled adult female ovary sample (RNA extracted using RNeasy MinElute kit, RACE conducted using SMARTer 5⍰/3⍰ RACE kit - Takara Bio, Kyoto, Japan). Visible bands were cloned using the NEB PCR cloning kit (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... Both types of DNA fragment (0.1 µg) were used as templates to synthesize RNA using an in vitro transcription kit (in vitro Transcription T7 Kit; Takara Bio, Shiga, Japan) in 1.5 mL centrifuge tubes ...
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bioRxiv - Molecular Biology 2020Quote: ... First-strand cDNA was synthesized with a PrimeScript™ RT synthesis kit with one-step genomic gDNA eraser kit according to instructions (TaKaRa, Tokyo, Japan). Gene-specific primer sets were designed based on the ORF ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted with a Bacterial RNA Extraction Kit and was immediately reverse-transcribed into cDNA using a PrimeScriptT™ RT-PCR Kit (TAKARA, Daliang, China). The common primer pair GSPilACF/GSPilACR was used to test genomic DNA contamination ...
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bioRxiv - Biochemistry 2023Quote: ... Mutants were generated by site-directed mutagenesis using a KOD-Plus Mutagenesis Kit (Toyobo, Japan) or PrimeSTAR® Mutagenesis Basal Kit (Takara Bio, Japan). For the expression ...
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bioRxiv - Developmental Biology 2020Quote: ... subsequently placed in a line in the electrode gap filled with 5 μl the mixture of 120 ng/μl Cas9 protein (TaKaRa, Japan), 300 ng/μl tracerRNA ...
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bioRxiv - Molecular Biology 2020Quote: ... The relative luciferase activity was determined by normalizing the firefly luciferase activity by the respective total EGFP protein amount quantified by Western blot using a GFP antibody (Clontech, 632460) in Image Studio ver 5.2 (LI-COR).
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bioRxiv - Biophysics 2021Quote: ... The rTetR protein sequence was subcloned from the Tet-On transactivator used in the commercially available Tet-On 3G system (Takara Bio). FUSN was derived from Addgene #122148 and GBP was based on a previously described construct (Rothbauer et al. ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293T cells were transfected with a pMXs-puro vector carrying hXkr8-GFP or a pCX4-bsr vector carrying hBSG cDNA tagged with HA or FLAG together with pGP for the gag-pol fusion protein (Takara Bio), and pCMV-VSV-G-RSV-Rev (RIKEN) ...
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bioRxiv - Neuroscience 2021Quote: Full-length human APP695 wild-type and mutant forms (APP:YFP, APPΔβ:YFP, APPGP:YFP) were inserted into pEYFP-N3 encoding the yellow fluorescent protein (YFP, Clontech, Mountain View, CA, U.S.). APPΔβ was generated by deletion of the juxtamembrane domain (aa 597-624 ...
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bioRxiv - Neuroscience 2020Quote: ... Nwd1 cDNAs corresponding to the N-terminal portion of the protein (accession number BC082552; 4bp–1026bp) were subcloned into pGBKT7 (Clontech Takara Bio) to express the N-terminal domain of Nwd1 fused with the GAL4 DNA-binding domain ...
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bioRxiv - Systems Biology 2020Quote: ... proteins in whole cell extract and immunoprecipitated fractions were digested with 2 mg/mL proteinase K (Takara Bio Inc., Kusatsu, Japan) at 42°C for 2 h ...
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bioRxiv - Neuroscience 2022Quote: ... protein lysate was immunoprecipitated for 2 h at 4°C with the following antibodies: mouse anti-GFP (1:1000; Takara Bio), mouse anti-FLAG (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... and the NES sequence of the HIV-1 rev protein into pT2ADW vector (Komatsu et al., 2018) by In-Fusion cloning (Takara Bio).
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bioRxiv - Developmental Biology 2022Quote: ... Co-IPs were carried out by incubating the samples with 30 μL of protein A agarose bead slurry for 4h at 4°C in a rotating wheel and with anti-mCherry (Takara 632496) of 1:1000 dilution ...
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bioRxiv - Plant Biology 2023Quote: ... His8-tagged soluble or insoluble CML recombinant proteins were purified at room temperature using Ni-NTA resin (His60, Takara Bio Inc.) using the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: The gene encoding the PP1c1-293 protein (herein simply referred to as PP1c) was cloned into the pOPIN-SC3 vector (OPPF) via In-Fusion Cloning (Takara Bio), which provides a cleavable N-terminal 6xHIS-SUMO tag for purification and solubility ...
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bioRxiv - Immunology 2023Quote: ... domain followed by an Avi-Tag(76) and a hexahistidine tag was cloned into a mammalian protein expression vector (pADD2) by In-Fusion (Takara Bio).
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bioRxiv - Evolutionary Biology 2024Quote: ... adhaerens Mint PDZ-1/PDZ-2/P1BM protein sequences (as described in the results section) were cloned into the mammalian bicistronic expression vector pIRES2-DsRed2 (Clontech, USA) to express recombinant proteins bearing C-terminal myc epitope tags (Figure 6K) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... HeLa cells were co-transfected to express each of EGFP-fused MK-D1 proteins with an endoplasmic reticulum (ER) localizing mCherry construct using the Xfect transfection reagent (Takara Bio). At 24 h post-transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... the U2OS 2-6-3 cells expressing degron-tagged LacI fusion proteins were incubated with 300 nM Shield1 ligand (Takara Bio) and 1 µg/mL doxycycline for 24 hours ...
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bioRxiv - Biochemistry 2024Quote: ... Recombinant His-tagged protein was purified from cell-free extracts using immobilized metal affinity chromatography (IMAC using Talon resin; Takara Bio). The buffer used during the purification process was 20 mM Tris-HCl ...
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bioRxiv - Systems Biology 2021Quote: ... We prepared ligation-free ribosome profiling and total RNA-seq libraries from the clarified polysome lysates treated for 6 hours following the instructions provided with their respective kits (smarter-seq smRNA-seq kit, Takara-Clontech; NEBnext Ultra-Directional II) augmented with our previously-published ligation-free ribosome profiling protocol42 ...
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bioRxiv - Cell Biology 2020Quote: ... Evo M-MLV RT Kit with gDNA Clean for qPCR (Accurate Biotech, Changsha, Hunan, China) and Mir-X™ miRNA First-Strand Synthesis Kit (Takara, Dalian, Liaoning, China) were applied for the transcription of RNA into complementary DNA (cDNA) ...
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bioRxiv - Microbiology 2020Quote: ... and reverse transcribed using the PrimeScript RT reagent kit (TaKaRa). The GPC segment was amplified and sequenced as previously described (9) ...
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bioRxiv - Cancer Biology 2021Quote: ... with SYBR Premix EX Taq kit (Takara Biotechnology Co., Ltd.). Primer sequences for RRM2 ...