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Citations for Takara Bio :
51 - 85 of 85 citations for Streptavidin magnetic beads since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: ... His6-tagged protein in the supernatant was captured on Talon Sepharose beads (#635502, Clontech/Takara) and pre-equilibrated against Buffer1 (300 mM NaCl ...
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Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: ... His6-tagged protein in the supernatant was captured on Talon Sepharose beads (#635502, Clontech/Takara) and pre-equilibrated against Buffer1 (300 mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... Fractions containing H1 were then incubated with 2mL bead volume of TALON affinity resin (Takara) for 1 hour at 4°C nutating ...
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bioRxiv - Biochemistry 2024Quote: ... Lysate was then incubated with TALON affinity resin (Takara, 1mL bead volume/1L bacterial culture) for 1hour at 4°C while nutating ...
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bioRxiv - Molecular Biology 2021Quote: ... in Terrific Broth and purified by metal ion affinity chromatography (IMAC) on TALON/Cobalt beads (Clontech) using 1M NaCl containing buffers to prevent the potential co-purification of E ...
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bioRxiv - Molecular Biology 2024Quote: The captured cDNA with beads was amplified using LA Taq DNA Polymerase Hot-Start (Takara, # RR042B) in 4x50μl reactions ...
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bioRxiv - Molecular Biology 2021Quote: ... Japan) for 20 min with zirconia beads in lysis buffer of the Nucleospin RNA plus (Takara Bio). Cell homogenates were then subjected to total RNA extraction with Nucleospin RNA plus (Takara Bio ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was applied onto a pre-equilibrated column containing Protino Glutathione Sepharose™ 4B beads (Takara) and kept for binding for 3 hrs at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... We coated 6-8 mg of 1.6 µm gold beads with 10-15 µg of EGFP-N1 (Clontech) or 20 μg pSuper-cofilin1-shRNA + 20 μg pSuper-ADF-shRNA (Bosch et al. ...
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Panacea: a hyperpromiscuous antitoxin protein domain for the neutralisation of diverse toxin domainsbioRxiv - Microbiology 2021Quote: ... Filtered lysate was incubated with 1 mL of previously buffer equilibrated Ni-beads (His60 Ni Superflow Resin, TaKaRa, Japan) for 30 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... coli cells induced with 100 mM IPTG and purified from bacterial extracts using TALON beads (Takara Bio, Catalog # 635502) according to the vendor’s instructions.
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bioRxiv - Microbiology 2021Quote: ... H6-Gfp-FtsZ and FtsZ were mixed in a 1:1 ratio for a final concentration of 8 µM in assembly buffer with GMPCPP (0.2 mM) and resuspended Talon Cobalt beads (Takara) and added ...
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bioRxiv - Biochemistry 2024Quote: ... The other 2A proteins were separated from the lysate using immobilized metal affinity chromatography with either TALON beads (Clontech) or chelating Sepharose beads charged with NiCl2 (Cytiva) ...
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bioRxiv - Biophysics 2024Quote: ... the solution of carboxylated beads was diluted into 400 µL of 1× Phosphate-Buffered Saline (PBS) (Takara Bio, T900) containing 100 mg/mL PLL-g-PEG (SuSoS ...
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bioRxiv - Microbiology 2024Quote: ... The protein-bead mixtures were then loaded onto a gravity column (TALON 2 mL Disposable Gravity Column, Takara #635606). Lysate was loaded into the column 5 times with gravity flow and then washed in a 1M NaCl buffer 3 times ...
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bioRxiv - Immunology 2024Quote: ... The lysate was incubated at 37°C for 5 min and subjected to IMAC purification using TALON beads (Clontech). The eluate was concentrated by ultrafiltration using 10 kDa cut-off Vivaspin (Sartorius ...
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bioRxiv - Molecular Biology 2021Quote: ... Beads were washed twice with Pulldown Buffer and ran on SDS-PAGE followed by western blotting (anti-His Clontech 631212).
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bioRxiv - Plant Biology 2021Quote: Tagmented DNA was amplified directly from the beads via 6 cycles of PCR amplification using the PrimeSTAR GXL Polymerase kit (Takara) and dual indexed adapters (Illumina ...
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bioRxiv - Physiology 2022Quote: ... the beads were well-washed by column binding buffer 5 times and then incubated in 2× Protein SDS PAGE Loading (Takara) 100°C for 5 min to completely elute the proteins ...
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bioRxiv - Genetics 2020Quote: ... The beads were then suspended in TdT reaction buffer (1× NEBuffer #4, 0.25 mM CoCl2, 15 U TdT (Takara Bio), 20 Ci α-32P-dCTP [6000 Ci/mmol]) ...
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bioRxiv - Biochemistry 2023Quote: ... Clarified lysate was then filtered through a 0.45 µm syringe filter and mixed with 2 mL of washed TALON® Metal Affinity Resin beads (Takara). The mixture was allowed to bind under nutation at 4℃ for 1 hr ...
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bioRxiv - Bioengineering 2024Quote: ... Proteins were expressed in suspension Expi293F cells at 37 °C and purified under native conditions with TALON metal affinity resin beads (Takara) and SEC experiments ...
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bioRxiv - Cell Biology 2020Quote: ... was added to the StrePD beads and incubated at 37 °C for 10 minutes followed by an incubation with Proteinase K (Takara, #9034) at 60 °C overnight ...
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bioRxiv - Developmental Biology 2022Quote: ... Co-IPs were carried out by incubating the samples with 30 μL of protein A agarose bead slurry for 4h at 4°C in a rotating wheel and with anti-mCherry (Takara 632496) of 1:1000 dilution ...
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bioRxiv - Genomics 2022Quote: ... The ligation reactions were heat-inactivated at 65°C for 10 minutes and cleaned up with NucleoMag NGS Clean-up and Size Select beads (Takara Bio) before electroporation into Endura Electrocompetent E ...
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bioRxiv - Neuroscience 2023Quote: ... lysates were centrifuged at 31,000 x g for 45 min at 4°C and the supernatant was mixed with nickel-nitrilotriacetic acid (Ni-NTA) beads (Takara, 635653) at 4°C for 2 h ...
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bioRxiv - Developmental Biology 2023Quote: ... The plate containing dried beads bound to single-cell DNA was extracted from the robot and processed following PicoPLEX® (Sopachem/Takara) protocol with half volumes as described in Macaulay et ...
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bioRxiv - Cancer Biology 2023Quote: ... A bead ratio of 1x was used (50 µL of AMPure XP beads to 50 µL cDNA PCR product with 1 µL of 10x lysis buffer added, as per Clontech instructions), and purified cDNA was eluted in 17 µL elution buffer provided by Clontech ...
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bioRxiv - Microbiology 2023Quote: ... The crude lysates were centrifuged at 35’000 g for 30 min and the cleared lysates incubated with 200 μL Ni-NTA agarose beads (Takara Bio, Shiga, Japan) for 1h at 4 °C ...
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bioRxiv - Microbiology 2024Quote: The purified product in ICDS method and captured beads in upgraded ICDS method were ready for Illumina library preparation with SMART ChIp-seq kit (Takara, Catalog # 634865) by following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: The purified product and captured beads in upgraded ICDS method were subjected to Illumina library preparation with SMART ChIp-seq kit (Takara, Catalog # 634865) by following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were incubated with beads for 24 hours before being transferred to a 24 well plate precoated with retronectin (Takara, Cat#: T100B). Retronectin coating was done by following the manufacturer’s protocol with approximately 10ug/well (5ug/cm^2 ...
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bioRxiv - Neuroscience 2023Quote: ... A bead ratio of 1x was used (25 μl of Sera-Mag Select beads to 25 μl cDNA PCR product with 0.5 μl of 10x lysis buffer added, as per Takara instructions at 0.5x volume), and purified cDNA was eluted in 17 μl elution buffer provided by Takara ...
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bioRxiv - Neuroscience 2024Quote: ... 12 cycles of amplification at PCR2 and clean-up of final library using NucleoMag NGS Clean-up and Size Select beads (Takara Bio Inc., Japan). Libraries were then quantified at the Genome Quebec Innovation Centre (Montreal ...
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bioRxiv - Neuroscience 2023Quote: ... 12 cycles of amplification at PCR2 (final RNA-seq library amplification) and clean-up of final library using NucleoMag NGS Clean-up and Size Select beads (Takara Bio Inc., Shiga, Japan). Libraries were then quantified at the Genome Quebec Innovation Centre (Montreal ...