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Citations for Takara Bio :
1 - 50 of 85 citations for Streptavidin magnetic beads since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 20 μL of TALON magnetic beads (Clontech), and 1 μg of the refolded RNA structure library were mixed in 1 mL of protein-binding buffer (10 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... and purified by MPG streptavidin beads (Takara, cat. 6124A) followed by RNAse (Promega ...
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bioRxiv - Plant Biology 2020Quote: ... then incubated with TALON magnetic beads (Clontech, cat# 35636) for 20 min at 4 °C ...
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bioRxiv - Plant Biology 2020Quote: ... then incubated with TALON magnetic beads (Clontech, cat# 35636) for 20 min at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... Amplified fragments were purified using 0.55x beads/sample ratio NucleoMag® NGS magnetic beads (Takara Bio). Amplicons were prepared for Oxford Nanopore sequencing using the ligation sequencing kit SQK-LSK110 (Oxford Nanopore Technologies ...
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bioRxiv - Genomics 2022Quote: ... followed by magnetic bead purification with NucleoMag NGS Clean-up and Size Select (Takara Bio). To prepare the CRISPEY-BAR middle insert for ligation ...
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bioRxiv - Biochemistry 2022Quote: ... pro-meprin α was activated by trypsin cleavage applying immobilized trypsin on magnetic beads (PT3957-1, Takara, buffer 30 mM Tris-HCl pH 7.4 ...
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bioRxiv - Bioengineering 2023Quote: ... The process was performed on a 200 μL scale using 160 μL of His60 Ni-magnetic beads suspension (Takara Bio, Shiga, Japan). The beads were washed twice with 500 μL of sterilized water and 500 μL of Equilibration buffer (20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Immunology 2021Quote: ... using the 1.5mL centrifuge tube magnetic stand (Takara, #621964). The final library concentrations and qualities were determined by both SYBR PCR (Kapa Biosystems ...
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bioRxiv - Bioengineering 2023Quote: ... GFP BrightComp eBeads™ Compensation Bead Kit (ThermoScientific) and mCherry Flow Cytometer Calibration Beads (Takara). Flow cytometry of stained T lymphocytes was then performed using a BD LSRFortessa Cell Analyzer (BD Biosciences ...
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bioRxiv - Biochemistry 2022Quote: Co2+-bound NTA-sepharose beads (Takara) were stripped off the metal ion with 100 mM EDTA and used as NTA beads or charged with 100 mM NiCl2 and used as NTA(Ni2+ ...
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bioRxiv - Plant Biology 2024Quote: ... A glutathione sepharose beads (635608, Takara)-based purification method was then used to purify the fusion protein ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was then mixed with 50 µL cobalt beads (TALON® Metal Affinity beads Clontech, 635502) prewashed three times in guanidium hydrochloride buffer and the samples incubated at RT for 3 h with rotation ...
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bioRxiv - Molecular Biology 2020Quote: ... The complex was purified using TALON beads (Clontech) after nucleic acid precipitation using 0.05% of PolyEthylImine (PEI ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatants were loaded on TALON beads (Takara Bio) pre-equilibrated with lysis buffer (1 ml of beads per 1 l of bacterial culture) ...
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bioRxiv - Biophysics 2023Quote: ... Supernatants were incubated with TALON Cobalt beads (Takara) for 2 hrs at RT and the proteins were eluted from beads using 50 mM sodium phosphate buffer with 150 mM imidazole at pH 7.4 ...
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bioRxiv - Cell Biology 2024Quote: ... Supernatant was incubated with TALON beads (Takara Clontech) for 1 h at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Supernatant was incubated with TALON beads (Takara Clontech) for 1 h at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... Bead-stimulated cells were transferred to Retronectin-coated (Takara) virus-containing plates and incubated overnight ...
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bioRxiv - Bioengineering 2022Quote: ... Bead-stimulated cells were transferred to Retronectin-coated (Takara) virus-containing plates and incubated overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... The eluate was then incubated with TALON beads (ClonTech). Beads were washed twice with P5 Buffer and once with P30 Buffer (P5 supplemented with 25 mM Imidazole) ...
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bioRxiv - Biochemistry 2021Quote: ... and applied to 3mL of Talon bead resin (Takara) that was equilibrated into lysis buffer ...
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bioRxiv - Bioengineering 2024Quote: ... Bead-stimulated cells were transferred to RetroNectin-coated (Takara) virus–containing plates and incubated overnight ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amounts of protein were incubated with TALON beads (Clontech) pre-equilibrated with purification buffer at RT for 1.5 h with overhead rotation ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was then incubated with 25μL NiNTA beads (Clontech) for 1 hour at 4°C for binding ...
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bioRxiv - Neuroscience 2020Quote: ... or in batch mode on TALON® beads (Takara Bio) collected on an EconoColumn (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... Plasmid pTAP-AFAP was constructed by cloning the gene fragment encoding AFAP-streptavidin-binding peptide-3xFLAG tag fusion protein (AFAP-SBP-3xFLAG, shorted as AFAP-SF) into mammalian expression vector pIRESpuro3 (Takara). SBP is a peptide with the length of 38 amino acids (Wilson et al. ...
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bioRxiv - Immunology 2021Quote: ... were incubated with 15 μL of Ni-NTA agarose beads (Takara) overnight at 4°C ...
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bioRxiv - Systems Biology 2020Quote: ... Samples were then incubated with TALON metal affinity resin beads (Takara) beads conjugated with 50 μg of Src SH2 domain32 and 16 μg of Fab derived from 4G10 V312 variant33 ...
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bioRxiv - Cell Biology 2021Quote: ... Clarified lysate was incubated with 5 mL TALON beads (Takara Bio), washed with 150 mL lysis buffer and eluted in 22 mL of elution buffer (25 mM Hepes pH 7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... Supernatants were loaded on 5 mL of TALON beads (Takara Bio) pre-equilibrated with the lysis buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated with Talon beads (Takara Bio Inc., Japan) for an hour at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Flow cytometry calibration beads for AcGFP1/eGFP and mCherry (Takara, 632594, 632595) were used as fluorescence intensity controls ...
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bioRxiv - Neuroscience 2021Quote: ... Cell extract is filtered and incubated with washed TALON beads (Takara 635501) at 4C for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech). Fractions by imidazole-elution were subjected to Hi-trap SP cation-exchange chromatography ...
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bioRxiv - Cell Biology 2020Quote: ... and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech). Fractions by imidazole-elution were subjected to Hi-trap SP cation-exchange chromatography ...
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bioRxiv - Microbiology 2023Quote: ... Clarified lysates were incubated with TALON Metal Affinity Resin cobalt beads (Takara), and beads were then washed with Tris-HCL (pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... The soluble fraction obtained was applied to Talon beads ( Cat # 635503; Takara) and incubated for 6 h at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... the selection was performed as described above with TALON beads (Takara Bio), 2.5 nmoles RNA and 250 pmoles of SPM-1 in selection.
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bioRxiv - Cell Biology 2020Quote: ... 4 °C for 45 min and the supernatants incubated with TALON beads (Clontech) pre-equlibrated with purification buffer at RT over night with overhead rotation followed by washing with wash buffer (8M urea ...
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bioRxiv - Genomics 2022Quote: ... cleaned up with NucleoMag NGS Clean-up and Size Select beads (Takara Bio), and ligated with the aforementioned triple-digested plasmid pool using T4 DNA ligase (New England Biolabs ...
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bioRxiv - Plant Biology 2020Quote: ... The remaining RT primers were digested on the beads with Exonuclease I (TAKARA) (37°C for 30 min ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was incubated with α-cMyc agarose beads (Takara Bio, Kyoto, Japan) on a rotary wheel for 16 h at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... for GST-tagged protein or on TALON-Cobalt beads column (635507, Takara Bio) for 6XHIS-tagged protein as previously described [19,26] ...
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bioRxiv - Biochemistry 2024Quote: ... After addition of DNaseI 1mM MgCl2 and 200μl 50% TALONR beads slurry (Clontech), the cell lysate was incubated at 10min at 4°C on a rolling shaker ...
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bioRxiv - Biochemistry 2023Quote: ... MA) and the protein was purified using Talon beads (Takara Bio Inc., Japan) following published protocols 37,38 ...
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bioRxiv - Immunology 2021Quote: ... and the supernatant was subjected to affinity chromatography using Ni-NTA beads column (Takara). Eluted protein was dialyzed against 1×PBS with four changes for two days at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant (800 µL) was incubated with 30 µL of Talon beads (Takara Bio) at 4°C for 1 hour with continuous shaking ...
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bioRxiv - Developmental Biology 2021Quote: ... beads were resuspended with 27 µl ddH2O and 1 µl 10× Ex-Taq buffer (TaKaRa). 1 µl proteinase K (Roche ...
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bioRxiv - Biochemistry 2020Quote: ... and the supernatant was mixed with 1 mL of TALON® metal affinity beads (Clontech) previously equilibrated with equilibration buffer (PBS pH 8 ...